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Citations for Promega :
1 - 50 of 8272 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... His-tagged and GST-tagged recombinant proteins were purified using Promega MagneHis™ Ni-Particles (Promega Cat#V854A) and MagneGST™ Glutathione Particles (Promega Cat# V861A ...
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bioRxiv - Cell Biology 2021Quote: ... Janelia Fluor 646 tagged Halo ligand (Promega) was used at 200nM final concentration to label SINAPS-mRNA in cells prior to fixation/imaging.
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bioRxiv - Biophysics 2023Quote: Transfected cells were stained with JF-549 tagged HaloTag ligand (Promega) and Vybrant DiO staining solution (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant GST-tagged MARK2 (Promega) was incubated with tau at 30 °C at 1:100 ratio of MARK2:tau in phosphorylation buffer (25 mM PIPES ...
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bioRxiv - Cancer Biology 2019Quote: CHO K-1 cells overexpressing hPD-L1 and the recombinant TCR ligand (hPD-L1 Antigen Presenting Cells, hPD-L1 aAPCs, Promega) and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE ...
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bioRxiv - Cell Biology 2021Quote: ... Halo-tagged proteins were labeled by incubating cells with 200nM HaloTag Ligand conjugated to Janelia Fluor 646 (Promega) for 30 min before imaging ...
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bioRxiv - Molecular Biology 2022Quote: ... His-tagged proteins were purified using Ni-NTA resin (Promega); bound proteins were washed with binding buffer and eluted into elution buffer (4 M urea ...
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bioRxiv - Cell Biology 2022Quote: Halo-3F-SOCS1 expression was induced with doxycycline overnight (1 μg/mL) and Halo-tagged protein detected by incubation with fluorescent Halo TMR ligand (10 nM; Promega) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in a medium containing 2 nM Janelia Fluor 646 (JF646) HaloTag ligand (Promega) and 0.5 μg/mL Hoechst33342 (Nacalai Tesque ...
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bioRxiv - Molecular Biology 2019Quote: ... 10.0 ng/ml recombinant human FGF (Promega) and 1.0 μM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were incubated with 1 nM Halo Tag TMR Ligand (G825A, Promega) at 37 °C for 2 h ...
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bioRxiv - Microbiology 2023Quote: ... cells were treated with JF646 Halo Ligand (Promega) for 24 hours before analysis on a BD Accuri C6 plus ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with HaloTag TMR Ligand (Promega) at a final concentration of 100 nM for 30 min incubation ...
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bioRxiv - Immunology 2020Quote: ... The transfected cells were labelled with HaloTag TMR ligand following manufacturer’s protocol (HaloTag® TMR Ligand Promega). Nuclear extract from transfected HEK293 cells (Halo-SP140 ...
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bioRxiv - Cell Biology 2021Quote: The Halo Ligands (Halo-TMR Ligand, Promega, Ref G8251) were added in the culture medium for 20 min at 100 nM ...
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bioRxiv - Cell Biology 2022Quote: ... The following commercially available cell-impermeable fluorescent ligands were utilized: HaloTag Fluorescent Ligand Alexa Fluor 488 (Promega, #G1002) in the following abbreviated as CA-Alexa488 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated with cell-permeant TMR red Halo-tag ligand (1:2,000 for 15 min, Promega) 24 hours post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated in 2 nM JF549-Halo-ligand (Cat. No. GA1110; Promega) for 15 minutes at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... Cell death was monitored using LDH-Glo Cyotoxicity Assay (Promega), CellToxGreen (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... cell death staining was done with the CellTox green (Promega) at a dilution of 0.25ul CellTox/500ul media for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were labelled with HT fluorescent ligand TMR (Promega) and sorted for stable HT expressors.
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bioRxiv - Cell Biology 2022Quote: ... cells were labeled with 200 nM JF646 ligand (Promega) for 15 min ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained with JF549 conjugated Halotag ligands (Promega) at 200nM concentration in 2% FBS/PBS for 20 min at RT ...
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bioRxiv - Cell Biology 2020Quote: ... TMRDirect ligand (Promega) was diluted 1:1000 in complete media and added to cells ...
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bioRxiv - Biophysics 2020Quote: HaloTag ligands (Promega): HaloTag TMR ...
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bioRxiv - Bioengineering 2023Quote: ... TMR Ligand (Promega) was diluted 1:200 in PBS and used as a 5x stock ...
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bioRxiv - Bioengineering 2023Quote: ... TMR Ligand (Promega) was diluted 1:200 in PBS and used as a 10x stock ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL of HaloTag TMR ligand (Promega, 5 mM) was added to 100 µL of JetABC (2.5 µM d-o-p ...
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bioRxiv - Cell Biology 2020Quote: ... cell death was assayed by release of LDH into cell culture media (Promega CytoTox 96® Non-Radioactive Cytotoxicity Assay) ...
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bioRxiv - Cancer Biology 2020Quote: ... the death of cells was detected using the Cell Toxicity Assay (CTxG, Promega). Fluorescence was measured using PerkinElmer Victor X3 Reader.
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bioRxiv - Bioengineering 2023Quote: HaloTagged AP-2 was visualized in the SUM159 cells through the addition of membrane-permeable JaneliaFluor646-HaloTag ligand (Promega) at a concentration of 125 nM ...
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bioRxiv - Microbiology 2021Quote: ... live cells were treated with cell-permeant Halotag TMR ligand (Promega, G8251) before paraformaldehyde fixation ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5ng ml-1 recombinant human fibroblast growth factor (rhFGF) (Promega, Cat#G5071, Madison, WI, USA), and 1% chicken embryonic extract (Seralab ...
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bioRxiv - Cancer Biology 2023Quote: HEK293T cells were transfected with a FLAG-tagged human DHHC20-expressing plasmid and a corresponding HA-tagged substrate-expressing plasmid using FuGENE transfection reagent (Promega) and incubated overnight ...
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bioRxiv - Cell Biology 2019Quote: ... Cell death was assessed by CellTox™ Green Cytotoxicity Assay (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell death was measured after 4 days using CytoTox Glo (Promega).
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bioRxiv - Immunology 2024Quote: ... Cell death was assessed using the CytoTox 96 Cytotoxicity assay (Promega) and IL1β secretion measured by ELISA (Invitrogen).
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: COS-7 cells grown in 10 cm plates to 70–80% confluence expressing Halo-tagged K560 or KIF5B (KHC) were labeled with TMR-HaloTag ligand (Promega, Madison, WI) 18–24 h post-transfection then lysed in P12 buffer (as described above ...
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bioRxiv - Biophysics 2023Quote: HeLa cells expressing each Halo-tagged chromatin remodeler were labeled with 5 nM Janelia Fluor® 549 (JF549) Halo-tag® ligand (GA1110, Promega) for 15 min at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated in 50 nM HaloTag TMR ligand (Promega) for 30 min and the medium was replaced with FluoroBrite (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated in 50 nM HaloTag TMR Ligand (Promega) for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated with Halo ligand Janelia Fluor 549 (Promega) at 25 nM for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Cells expressing HaloTag fusions were labelled with TMR ligand (Promega) following the procedure previously described in [54] ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were labeled with 1µM HaloTag Oregon Green Ligand (Promega) according to the manufacturer’s instructions and both the HALO- and eGFP-tagged cell lines were sorted again to enrich for cells expressing the HALO- or eGFP-tagged SHARP rescue constructs (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... Cells expressing HaloTag were treated with JF646 HaloTag ligand (Promega; 1 μM final concentration in growth medium ...
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bioRxiv - Molecular Biology 2019Quote: ... Halo618 ligand (Promega, N1663) was added and incubated at 37°C for 5 hours ...
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bioRxiv - Cell Biology 2023Quote: ... HaloTag TMR Ligand (Promega) was added at a final concentration of 100 nM ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μL 3.5 mM Alexa Fluor 660 HaloTag ligand (Promega) was added to 200-300 μL cells and incubated at 30°C for 5 minutes ...
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bioRxiv - Biophysics 2022Quote: ... and labelled with 1 µM TMR-HaloTag ligand (Promega, G8251) and 0.12 µM SiR-SNAP-Tag ligand (NEB ...
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bioRxiv - Biophysics 2023Quote: ... we stained the cells with 1 ml of a 1:2,000 TMR Halo-ligand solution (Promega, Cat. No.: G8251) to fluorescently label the Halo-tagged proteins ...