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Citations for Promega :
1 - 50 of 1197 citations for Recombinant Human Fms related Tyrosine Kinase 3 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... His-tagged and GST-tagged recombinant proteins were purified using Promega MagneHis™ Ni-Particles (Promega Cat#V854A) and MagneGST™ Glutathione Particles (Promega Cat# V861A ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant GST-tagged MARK2 (Promega) was incubated with tau at 30 °C at 1:100 ratio of MARK2:tau in phosphorylation buffer (25 mM PIPES ...
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bioRxiv - Cell Biology 2023Quote: ... 10mM Ultrapure ATP and 0.7 μg of recombinant full-length human CDC7/DBF4 kinase (Promega, V5088). Reactions were incubated at 37 °C for 120 min and then terminated by freezing on dry ice before mass spectrometry.
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bioRxiv - Microbiology 2024Quote: Recombinant FIKK kinase domains activity was measured using the ADP-Glo kinase assay (Promega), which quantifies the amount of ADP produced during the kinase reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... His-tagged proteins were purified using Ni-NTA resin (Promega); bound proteins were washed with binding buffer and eluted into elution buffer (4 M urea ...
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bioRxiv - Molecular Biology 2019Quote: ... 10.0 ng/ml recombinant human FGF (Promega) and 1.0 μM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected with FLAG-tagged Nlgn1 or GFP-tagged NGL-3 using Fugene6 (Promega, Cat #E2691). Transfected HEK293T cells were mechanically dissociated 24 hr after transfection and co-cultured with DIV10 cortical neurons prepared from C57BL/6J Sorcs1flox/flox mice for 16 hr.
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bioRxiv - Biochemistry 2021Quote: To investigate enzymatic activity of recombinant PfGSK3 a commercial luminescence-based kinase assay (KinaseGlo Plus, Promega) was used as previously described (93) ...
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bioRxiv - Microbiology 2021Quote: ... Remaining ATP was readout after 3 h incubation using Kinase-Glo® Luminescent Kinase kit following manufacturer’s instructions (Promega, WI, USA).
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bioRxiv - Cancer Biology 2023Quote: HEK293T cells were transfected with a FLAG-tagged human DHHC20-expressing plasmid and a corresponding HA-tagged substrate-expressing plasmid using FuGENE transfection reagent (Promega) and incubated overnight ...
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bioRxiv - Genomics 2022Quote: ... 10 ng/mL recombinant human basic fibroblast growth factor (Promega), and 1 μM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2021Quote: ... and 5 ng/ml recombinant human fibroblast growth factor (G5071, Promega).
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bioRxiv - Cell Biology 2019Quote: ... and 5 ng/mL recombinant human basic fibroblast growth factor (bFGF, Promega) then centrifuged ...
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bioRxiv - Microbiology 2022Quote: ... Kinase activity was measured using ADP-Glo Kinase assay (V6930, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... In vitro kinase reactions using purified CDK1/CyclinA2 kinase (Promega V2961) were carried out in a 25 μl reaction containing 100 ng of 6His-MSH-5 ...
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bioRxiv - Neuroscience 2021Quote: ... HA-tagged NPHP1 expression vectors were transfected to mIMCD-3 cells using FuGENE HD (Promega) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... 3) goat anti-human H+L (Promega, W403B) at 1:3,000 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... using the Kinase-Glo® Luminescent Kinase Assay (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The non-radioactive Kinase-Glo® Luminescent Kinase Assays (Promega, Cat # V6711) was used to measure ATP consumption in the presence of its natural substrate glucose ...
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bioRxiv - Biochemistry 2022Quote: ... ~2.5 μg recombinant bacmid DNA and 3 μl FuGENE HD Transfection reagent (Promega) in 100 μl Sf900 II media (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... A Kinase-Glo (Promega) assay kit for kinase activity was used to determine the activity of the purified PanK under different conditions 16.
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bioRxiv - Immunology 2019Quote: The TBK1 kinase assay was performed using the TBK1 Kinase Enzyme System (Promega) and ADP-Glo Kinase Assay kit (Promega ...
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bioRxiv - Cancer Biology 2020Quote: ATP release was measured using the Kinase-Glo® Luminescent Kinase Assay (Promega) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Other substrates for kinases were included in kinases kits from Promega (Madison, WI). Total protein kit ...
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bioRxiv - Neuroscience 2023Quote: ... followed by kinase assays run using the ADP-Glo Kinase Assay kit (Promega). After the termination of kinase assay ...
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bioRxiv - Biochemistry 2023Quote: Kinase assays were conducted using an ADP-Glo™ Kinase Assay Kit (Promega). Reagents were prepared as described in the kit ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Initial P0 virus was obtained by addition of ∼3 µg recombinant bacmid DNA mixed with 3 µl FuGENE HD Transfection reagent (Promega) in 100 µl Sf900 II media (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5ng ml-1 recombinant human fibroblast growth factor (rhFGF) (Promega, Cat#G5071, Madison, WI, USA), and 1% chicken embryonic extract (Seralab ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The kinase inhibition assay was done using the Kinase Glo(R) kit from Promega similar to published procedures ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 mg of His-Tev-HaloTag-(3C)-human γ-TuNA was coupled to Halo Magne beads (Promega). Xenopus laevis egg extract was prepared by standard methods28 ...
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bioRxiv - Microbiology 2021Quote: ... GSK3β kinase enzyme system (Promega) was used as per manufactures protocol with following modifications ...
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bioRxiv - Biochemistry 2022Quote: Kinases were purchased from Promega (SRPK1 ...
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bioRxiv - Cell Biology 2022Quote: ... The ATP in samples was quantified using the Kinase-Glo Max Luminescence Kinase Assay (Promega) and a Beckman Coulter’s Paradigm Plate Reader ...
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bioRxiv - Biochemistry 2021Quote: The kinase activity of PKAc was measured using the ADP-Glo kinase kit (V9101; Promega) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Kinase reactions were then stopped and processed using ADP-Glo kinase assay kit (Promega #V6930) as indicated in the manual ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cell surface levels of HiBiT-tagged human S1PR1 were monitored using a Nano Glo HiBiT Extracellular Detection System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: Transient transfection of the dual NanoLuc and Halo-Tagged tagged BRD4Tandem plasmid (Promega) was performed as described previously52 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and Bone Morphogenetic Protein 4 (BMP4, 10 ng/ml, Bio-Techne) plus phosphoinositide 3-kinase inhibitor Ly294002 (10 µM, Promega). After 42 h incubation at 37°C ...
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bioRxiv - Microbiology 2022Quote: The ADP-GloTM Kinase assay (Promega) was utilized to measure the turn-over of ATP to ADP in ClpC1-mediated casein degradation assays according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and T4 polynucleotide kinase (Promega, M4103) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2019Quote: ... Kinase detection solution (10 μL) (Promega) was added and incubated for 15 minutes at room temperature and luminescence was measured on a plate reader.
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bioRxiv - Cancer Biology 2019Quote: FAK1 kinase domain (AA393-698) (Promega) (25 μg ...
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bioRxiv - Molecular Biology 2022Quote: ... and ADP-Glo kinase assay (Promega), in accordance with the manufacturers’ instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and ADP-GloTM Kinase Assay (Promega). By measuring luciferase activity using the Ensight Multimode Plate Reader (PerkinElmer) ...
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bioRxiv - Biochemistry 2024Quote: ... using ADP-Glo Kinase Assay (Promega). Briefly ...
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bioRxiv - Genetics 2019Quote: ... A cDNA encoding Myc epitope-tagged human TRAPα was synthesized by Integrated DNA Technologies (IDT) and was subcloned into the pTarget mammalian expressing vector (Promega).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were co-transfected with pcDNA5/FRT construct encoding haemagglutinin (HA)-tagged human CB1 receptor cDNA and pOG44 (Flp recombinase plasmid) using transfection reagent Fugene HD (Promega) as previously described for AtT-20 pituitary tumour cells [26] ...
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bioRxiv - Biochemistry 2022Quote: ... 1200 ng of ORs tagged with the first 20 amino acids of human rhodopsin (rho-tag) at the N-terminal ends53 in pCI (Promega) and 30 ng eGFP were transfected using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: In vitro kinase assays were performed as described in ADP-Glo™ Kinase assay kit (Promega, Germany) with minor modifications ...
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bioRxiv - Cell Biology 2022Quote: ... The synthetized ATP was quantified using a luciferin/luciferase assay (Kinase-Glo Max Luminescence Kinase Assay, Promega) in a Beckman Coulter Paradigm plate reader ...