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Citations for Promega :
1 - 50 of 1351 citations for Recombinant E. coli MUG Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Competent Escherichia coli cells (E. coli JM109; Promega, Fitchburg, USA) were incubated with the ligation mixture on ice for 30 min and then transformed by a 45 sec heat shock at 42 °C ...
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bioRxiv - Plant Biology 2020Quote: ... Protein for MUG and LUC assays was extracted on ice in 1x passive lysis buffer (PLB: Promega). MUG assays were performed by adding 40μl of protein extract to 100μl of MUG assay buffer (2mM MUG ...
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bioRxiv - Genetics 2019Quote: ... Purified recombinant luciferase protein (Promega) was used to generate a standard curve.
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bioRxiv - Microbiology 2022Quote: ... Recombinant EbfC was expressed in Escherichia coli Rosetta II cells and purified using MagneHis Particles (Promega) as previously described (24 ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified using MagneHis nickel particles (Promega, WI), then dialyzed against EMSA buffer (50mM Tris-HCl ...
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bioRxiv - Bioengineering 2023Quote: ... several concentrations of purified recombinant HaloTag protein standard (Promega, G4491) were labeled with ligand and run in duplicate to generate a calibration curve ...
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bioRxiv - Immunology 2024Quote: Recombinant antibodies were purified using protein G magnetic beads (Promega #G7472), HiTrap protein G HP columns (Cytiva #17040401) ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids were eluted with RNase free water and used as templates for in vitro gene expression (E. coli S30 Extract System for Circular DNA, Promega).
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmids were resuspended in RNase-free water and used as templates for in vitro gene expression (E. coli S30 Extract System for Circular DNA, Promega). Reactions were set up by adding 1 μL of 0.25 mM amino acid mix ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified with MagneGST™ Protein Purification System (www.promega.com/protocols/; Promega, Madison, WI, USA).
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bioRxiv - Plant Biology 2024Quote: ... The recombinant GST-OsNAC5 fusion protein was subsequently purified with the MagneGST™ Protein Purification System (Promega), following the provided protocol.
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bioRxiv - Biochemistry 2021Quote: ... Proteins were reduced and alkylated prior to digestion with recombinant LysC (Promega) and trypsin (Promega) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were reduced and alkylated prior to digestion with recombinant LysC (Promega) and trypsin (Promega) ...
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bioRxiv - Microbiology 2023Quote: The effect of pyocin SX2 on protein synthesis was assessed by an in vitro transcription-translation assay (E. coli T7 S30 Extract System for Circular DNA. Promega, Germany). pRL-SV40 vector (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... Protein for the 4-methylumbelliferyl-b-D-glucuronide (MUG) and luciferase (LUC) assays was extracted in 1x passive lysis buffer (PLB: Promega). MUG assays were performed by adding 40 mL of protein extract to 100 mL of MUG assay buffer [2 mM MUG ...
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bioRxiv - Developmental Biology 2019Quote: ... we produced recombinant H3 mutant proteins from mRNAs using rabbit reticulocyte lysate (Promega L4600). After 3h of incubation at 4°C in interphase extracts followed by another 3h incubation with anti-HA beads ...
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bioRxiv - Developmental Biology 2020Quote: ... and 200 ng purified recombinant mouse DLX2 protein in Gel Shift Binding Buffer (Promega). Unlabeled probe was added for ‘cold’ competition ...
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bioRxiv - Biochemistry 2024Quote: ... The HaloTag-JF503-SWSAP1 was quantified by using recombinant HALO-GFP protein standard (Promega) pre-incubated with JF503 at a 1:1 molar ratio and titrated into single molecule buffer (20 mM Tris pH7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng/μl of mRNA and 6 ng/μl QuantiLum Recombinant Luciferase protein (Promega) were coinjected ...
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bioRxiv - Developmental Biology 2021Quote: ... coli (Promega).
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bioRxiv - Developmental Biology 2021Quote: ... coli (Promega), and plasmid minipreps were prepared using the alkaline miniprep protocol by Wang et al ...
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bioRxiv - Molecular Biology 2019Quote: ... coli (Promega) were transformed with the expression vector ...
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bioRxiv - Biophysics 2019Quote: ... coli (Promega) or clinical isolate E ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli (Promega) were prepared using a Z-Competent E ...
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bioRxiv - Molecular Biology 2024Quote: ... coli (Promega) and grown on LB + 100 μg/ml carbenicillin (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... Buffer E (Promega) was added in cDNA and heated at 95 °C for 2 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... coli and HeLa cells as well as of a commercially available yeast protein digest (Promega), The ratios of the peptide amount of each species within the three mixtures are given in Table S1.
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bioRxiv - Systems Biology 2019Quote: Cells were lysed and recombinant proteins were isolated using Magne® HaloTag® Beads (Promega) as previously described (15) ...
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bioRxiv - Biochemistry 2020Quote: ... coli KRX (Promega) were used for plasmid propagation and for protein production ...
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bioRxiv - Biochemistry 2020Quote: ... coli KRX (Promega) at 16°C using 0.1% l-rhamnose as inducer ...
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bioRxiv - Molecular Biology 2021Quote: ... coli KRX (Promega) which is a K12 derivative was used to express His6-Tus-GFP ...
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bioRxiv - Cell Biology 2021Quote: ... coli (Promega, L1195) and selected on LB plates containing 0.1 mg/ml ampicillin at 37°C overnight ...
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bioRxiv - Bioengineering 2022Quote: ... coli (Promega, JM109), and plated on LB-Ampicillin plates ...
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bioRxiv - Biophysics 2019Quote: ... coli cells (Promega) as described by Shen et al ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... coli DH5α (Promega) (cf ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cells (Promega) in 2xYT media with 25 µg/mL kanamycin and 2mM MgSO4 at 37°C to OD600 of 0.7–0.9 ...
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bioRxiv - Physiology 2019Quote: ... coli JM109 (Promega) cells ...
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bioRxiv - Microbiology 2020Quote: ... coli JM109 (Promega) was used for glutamate transporter complementation assays ...
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bioRxiv - Microbiology 2022Quote: ... coli JM109 (Promega), and E ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli KRX (Promega) cells were transformed by heat shock with the pET-25b+-RSL-MOA plasmid and pre-cultured in LB media substituted with 50 μg/mL ampicillin at 37°C under agitation at 180 rpm overnight ...
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bioRxiv - Bioengineering 2023Quote: ... coli (Promega, JM109), and plated on LB-Ampicillin plates ...
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bioRxiv - Bioengineering 2023Quote: ... coli (Promega, JM109), and plated on LB-Ampicillin plates ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... coli (Promega, Madison, WI) and isolated in large quantities using a ZymoPURE-EndoZero Maxiprep kit (Zymo Research ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli JM109 strain (Promega) was transformed with the violacein pathway plasmid (pTU2S(VioAE)-b-VioBCD ...
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bioRxiv - Plant Biology 2020Quote: ... coli KRX cells (Promega) was performed via heat shock method and subsequent selection on LB plates containing 50 mg/L kanamycin ...
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bioRxiv - Immunology 2022Quote: ... coli (Promega, Walldorf, Germany). Bacteria were grown over night on LB/amp/IPTG/X-gal plates and non-colored colonies were selected for plasmid preparation ...
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bioRxiv - Molecular Biology 2020Quote: ... coli JM109 (Promega L2005). Plasmids were isolated and purified from bacteria ...
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bioRxiv - Cell Biology 2021Quote: ... coli strain KRX (Promega) and cultured in LB medium containing 100 µg/ml ampicillin ...
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bioRxiv - Microbiology 2021Quote: ... coli DH5α cells (Promega). Plasmids were extracted and sequenced with primers 3-12 (see Table S1 ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli KRX (Promega, WI) following manufacturer’s protocol ...