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Citations for Promega :
1 - 50 of 348 citations for Recombinant E. coli LT IIa His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... His-tagged and GST-tagged recombinant proteins were purified using Promega MagneHis™ Ni-Particles (Promega Cat#V854A) and MagneGST™ Glutathione Particles (Promega Cat# V861A ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant GST-tagged MARK2 (Promega) was incubated with tau at 30 °C at 1:100 ratio of MARK2:tau in phosphorylation buffer (25 mM PIPES ...
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bioRxiv - Genetics 2020Quote: ... Competent Escherichia coli cells (E. coli JM109; Promega, Fitchburg, USA) were incubated with the ligation mixture on ice for 30 min and then transformed by a 45 sec heat shock at 42 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... His-tagged proteins were purified using Ni-NTA resin (Promega); bound proteins were washed with binding buffer and eluted into elution buffer (4 M urea ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant EbfC was expressed in Escherichia coli Rosetta II cells and purified using MagneHis Particles (Promega) as previously described (24 ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids were eluted with RNase free water and used as templates for in vitro gene expression (E. coli S30 Extract System for Circular DNA, Promega).
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmids were resuspended in RNase-free water and used as templates for in vitro gene expression (E. coli S30 Extract System for Circular DNA, Promega). Reactions were set up by adding 1 μL of 0.25 mM amino acid mix ...
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bioRxiv - Microbiology 2023Quote: The effect of pyocin SX2 on protein synthesis was assessed by an in vitro transcription-translation assay (E. coli T7 S30 Extract System for Circular DNA. Promega, Germany). pRL-SV40 vector (Promega ...
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bioRxiv - Biochemistry 2023Quote: Transient transfection of the dual NanoLuc and Halo-Tagged tagged BRD4Tandem plasmid (Promega) was performed as described previously52 ...
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bioRxiv - Developmental Biology 2021Quote: ... coli (Promega).
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bioRxiv - Developmental Biology 2021Quote: ... coli (Promega), and plasmid minipreps were prepared using the alkaline miniprep protocol by Wang et al ...
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bioRxiv - Molecular Biology 2019Quote: ... coli (Promega) were transformed with the expression vector ...
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bioRxiv - Biophysics 2019Quote: ... coli (Promega) or clinical isolate E ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli (Promega) were prepared using a Z-Competent E ...
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bioRxiv - Molecular Biology 2024Quote: ... coli (Promega) and grown on LB + 100 μg/ml carbenicillin (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... Buffer E (Promega) was added in cDNA and heated at 95 °C for 2 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant Halo-His-gene was expressed using a 30 μL TNT®SP6 High-Yield Wheat Germ Protein Expression System (Promega, USA, Catalog No L3261). Expression levels were determined by SDS-PAGE and western blot ...
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bioRxiv - Microbiology 2021Quote: ... HA-tagged DDX1and HA-tagged TRIF were constructed by inserting indicated sequences into pCMV-HA (Promega), respectively ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected with FLAG-tagged Nlgn1 or GFP-tagged NGL-3 using Fugene6 (Promega, Cat #E2691). Transfected HEK293T cells were mechanically dissociated 24 hr after transfection and co-cultured with DIV10 cortical neurons prepared from C57BL/6J Sorcs1flox/flox mice for 16 hr.
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged or HA-tagged protein was detected using a commercially available mouse monoclonal antibody (Promega).
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bioRxiv - Biochemistry 2020Quote: ... coli KRX (Promega) were used for plasmid propagation and for protein production ...
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bioRxiv - Biochemistry 2020Quote: ... coli KRX (Promega) at 16°C using 0.1% l-rhamnose as inducer ...
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bioRxiv - Molecular Biology 2021Quote: ... coli KRX (Promega) which is a K12 derivative was used to express His6-Tus-GFP ...
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bioRxiv - Cell Biology 2021Quote: ... coli (Promega, L1195) and selected on LB plates containing 0.1 mg/ml ampicillin at 37°C overnight ...
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bioRxiv - Bioengineering 2022Quote: ... coli (Promega, JM109), and plated on LB-Ampicillin plates ...
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bioRxiv - Biophysics 2019Quote: ... coli cells (Promega) as described by Shen et al ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... coli DH5α (Promega) (cf ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cells (Promega) in 2xYT media with 25 µg/mL kanamycin and 2mM MgSO4 at 37°C to OD600 of 0.7–0.9 ...
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bioRxiv - Physiology 2019Quote: ... coli JM109 (Promega) cells ...
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bioRxiv - Microbiology 2020Quote: ... coli JM109 (Promega) was used for glutamate transporter complementation assays ...
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bioRxiv - Microbiology 2022Quote: ... coli JM109 (Promega), and E ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli KRX (Promega) cells were transformed by heat shock with the pET-25b+-RSL-MOA plasmid and pre-cultured in LB media substituted with 50 μg/mL ampicillin at 37°C under agitation at 180 rpm overnight ...
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bioRxiv - Bioengineering 2023Quote: ... coli (Promega, JM109), and plated on LB-Ampicillin plates ...
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bioRxiv - Bioengineering 2023Quote: ... coli (Promega, JM109), and plated on LB-Ampicillin plates ...
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bioRxiv - Cell Biology 2021Quote: ... Janelia Fluor 646 tagged Halo ligand (Promega) was used at 200nM final concentration to label SINAPS-mRNA in cells prior to fixation/imaging.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... coli (Promega, Madison, WI) and isolated in large quantities using a ZymoPURE-EndoZero Maxiprep kit (Zymo Research ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli JM109 strain (Promega) was transformed with the violacein pathway plasmid (pTU2S(VioAE)-b-VioBCD ...
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bioRxiv - Plant Biology 2020Quote: ... coli KRX cells (Promega) was performed via heat shock method and subsequent selection on LB plates containing 50 mg/L kanamycin ...
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bioRxiv - Immunology 2022Quote: ... coli (Promega, Walldorf, Germany). Bacteria were grown over night on LB/amp/IPTG/X-gal plates and non-colored colonies were selected for plasmid preparation ...
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bioRxiv - Molecular Biology 2020Quote: ... coli JM109 (Promega L2005). Plasmids were isolated and purified from bacteria ...
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bioRxiv - Cell Biology 2021Quote: ... coli strain KRX (Promega) and cultured in LB medium containing 100 µg/ml ampicillin ...
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bioRxiv - Microbiology 2021Quote: ... coli DH5α cells (Promega). Plasmids were extracted and sequenced with primers 3-12 (see Table S1 ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli KRX (Promega, WI) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... coli strains JM109 (Promega) and S17-1λpir/pLFX (E ...
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bioRxiv - Genetics 2020Quote: Escherichia coli JM109 (Promega) was used to propagate plasmids for sequencing analysis ...
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bioRxiv - Microbiology 2022Quote: ... coli DH5α cells (Promega). Plasmids were extracted and sequenced using Sanger sequencing.
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bioRxiv - Biochemistry 2024Quote: ... coli KRX cells (Promega) were transformed with the plasmid and selected on an LB agar plate supplemented with 50 μg/ml kanamycin ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant (rTdT) enzyme (Promega). Cells were then incubated at 37 °C for 60 min ...
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bioRxiv - Immunology 2022Quote: ... Ectopic Flag-tagged Galectin 8 or HA-tagged LILRB4 plasmid were transfected into the Mc38/B16/HEK294 cells with Fugene (Promega). A blank vector control was applied ...
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bioRxiv - Cancer Biology 2023Quote: HEK293T cells were transfected with a FLAG-tagged human DHHC20-expressing plasmid and a corresponding HA-tagged substrate-expressing plasmid using FuGENE transfection reagent (Promega) and incubated overnight ...