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Citations for Promega :
1 - 50 of 86 citations for Rcombinant Cynomolgus IL2RA Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Total RNA of the renal cortex in cynomolgus monkeys was extracted using Maxwell RSC simplyRNA tissue kit (Promega, AS1340) by an automated Maxwell RSC Instrument (Promega) ...
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bioRxiv - Cancer Biology 2019Quote: ... rat or cynomolgus ICOS as target cells were co-incubated with ADCC reporter cells expressing human FcγRIIIa (V158; Promega) at a 5:1 ratio ...
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bioRxiv - Biochemistry 2023Quote: Transient transfection of the dual NanoLuc and Halo-Tagged tagged BRD4Tandem plasmid (Promega) was performed as described previously52 ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant GST-tagged MARK2 (Promega) was incubated with tau at 30 °C at 1:100 ratio of MARK2:tau in phosphorylation buffer (25 mM PIPES ...
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bioRxiv - Microbiology 2021Quote: ... HA-tagged DDX1and HA-tagged TRIF were constructed by inserting indicated sequences into pCMV-HA (Promega), respectively ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected with FLAG-tagged Nlgn1 or GFP-tagged NGL-3 using Fugene6 (Promega, Cat #E2691). Transfected HEK293T cells were mechanically dissociated 24 hr after transfection and co-cultured with DIV10 cortical neurons prepared from C57BL/6J Sorcs1flox/flox mice for 16 hr.
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged or HA-tagged protein was detected using a commercially available mouse monoclonal antibody (Promega).
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bioRxiv - Plant Biology 2022Quote: ... His-tagged and GST-tagged recombinant proteins were purified using Promega MagneHis™ Ni-Particles (Promega Cat#V854A) and MagneGST™ Glutathione Particles (Promega Cat# V861A ...
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bioRxiv - Cell Biology 2021Quote: ... Janelia Fluor 646 tagged Halo ligand (Promega) was used at 200nM final concentration to label SINAPS-mRNA in cells prior to fixation/imaging.
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bioRxiv - Immunology 2022Quote: ... Ectopic Flag-tagged Galectin 8 or HA-tagged LILRB4 plasmid were transfected into the Mc38/B16/HEK294 cells with Fugene (Promega). A blank vector control was applied ...
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bioRxiv - Cancer Biology 2023Quote: HEK293T cells were transfected with a FLAG-tagged human DHHC20-expressing plasmid and a corresponding HA-tagged substrate-expressing plasmid using FuGENE transfection reagent (Promega) and incubated overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... C-terminal large bit (LgBiT) tagged construct (Promega) which is transcribed under control of the HSV-TK promoter.
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bioRxiv - Molecular Biology 2023Quote: ... N-terminal small bit (SmBiT) tagged construct (Promega), with the native HSV-TK promoter swapped out for a CMV promoter ...
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bioRxiv - Cell Biology 2023Quote: ... the HaloTag-tagged SUV420H2 expression vector (FHC26822; Promega) was used as a template for PCR amplification of the target regions ...
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bioRxiv - Cell Biology 2023Quote: The HaloTag-tagged KDM4D expression vector (FHC06842; Promega), the PB533-based H2B-Halo expression vector (Uchino et al. ...
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bioRxiv - Developmental Biology 2019Quote: ... Tagged-β-Catenin and Tagged-β-Catenin mutants were synthesized using the TNT coupled reticulocyte lysate system according to the manual (Promega, L5020, USA). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... His-tagged proteins were purified using Ni-NTA resin (Promega); bound proteins were washed with binding buffer and eluted into elution buffer (4 M urea ...
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bioRxiv - Biochemistry 2023Quote: ... Luminescence from HiBiT-tagged proteins was measured with GloMax Navigator (Promega). Luminescence from CMs and lysates of nontransfected cells were used for background correction.
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bioRxiv - Biophysics 2023Quote: Transfected cells were stained with JF-549 tagged HaloTag ligand (Promega) and Vybrant DiO staining solution (Invitrogen ...
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bioRxiv - Biophysics 2024Quote: ... HALO-tagged miniGs was labeled using HALO-JaneliaFluor595 (HALO-JF595) (Promega) by incubating cells in ∼2 μM of HALO-JF595 in DMEM supplemented with 10% FBS for 30 min at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HiBit-tagged proteins were detected using the NanoGlo HiBit Blotting kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... HiBiT-tagged proteins were detected using the Nano-Glo HiBiT blotting system (Promega) according to the manual ...
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bioRxiv - Biochemistry 2023Quote: Stable COMT cell lines were transfected with Myc-tagged ubiquitin using FugeneHD (Promega) following the manufacturer’s instructions using 4 μg plasmid ...
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bioRxiv - Neuroscience 2021Quote: ... Myc-tagged MiD49 and MiD51 were produced via in vitro transcription/translation (IVT) (Promega). For in vitro ubiquitination assays ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were transiently transfected with tagged-target proteins using Fugene® HD (Promega) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... by PCR and cloned into the Halo-tagged pFN21A vector (Promega, Madison, WI, USA). The AMPKγ1 mutant vector wherein Ser260 and Thr262 were replaced with alanine was created using parent-template backbone mouse AMPKγ1- full-length vector (# 15996 ...
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bioRxiv - Biochemistry 2023Quote: ... 10 nmol of HALO-tagged UBDs were immobilized on 100 μl HALOLink resin (Promega) in 500 μl HALO-coupling buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... and 75 μg of Rho-tagged OR in the pCI mammalian expression vector (Promega) were transfected 18 to 24 h before odorant stimulation using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... and 75 μg of rho-tagged OR in the pCI mammalian expression vector (Promega) were transfected 18–24 h before odorant stimulation using Lipofectamine 2000 (11668019 ...
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bioRxiv - Molecular Biology 2024Quote: ... GST-tagged AtCFI59 was purified using agarose beads (MagneGSTTM Glutathione Particles; Promega, Madison, USA) and retained on the beads to pull-down purified HIS-tagged AtCFI25a protein in TBS buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cancer Biology 2021Quote: ... 19.5µl of nuclease-free water) (FC-121-1030, Illumina; G9441, Promega). Transposition reactions were incubated at 37°C for 30 minutes in an Eppendorf ThermoMixer with agitation at 1000 RPM ...
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bioRxiv - Molecular Biology 2020Quote: A Myc-tagged construct was synthesized by Genewiz and cloned into the psiCHECK2 vector (Promega), replacing the synthetic Renilla Luciferase gene ...
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bioRxiv - Biochemistry 2022Quote: ... with or without RFP-tagged full-length Caskin1 using ViaFect Transfection Reagent (Promega, Madison, WI) as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... HA-tagged NPHP1 expression vectors were transfected to mIMCD-3 cells using FuGENE HD (Promega) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Myc-tagged DHX36 was constructed by inserting the ORF of DHX36 into pCMV-Myc (Promega). pHAGE-WT and -D126A DDX21 were constructed by inserting the flag-tagged WT and D126A DDX21 into pHAGE-bsd ...
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bioRxiv - Biochemistry 2022Quote: ... The preparation of Halo-tagged NEMO and its covalent attachment to Halo-Link Resin (Promega) have been described (Emmerich et al. ...
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bioRxiv - Systems Biology 2023Quote: ... Halo-tagged TFs were expressed with TnT® Coupled Wheat Germ Extract System (Promega, USA) using 2 µg plasmid DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... purified Halo-tagged MultiDsk (15 μg per condition) was pre-incubated with HaloLink resin (Promega) for 1 h with rotation at room temperature in binding buffer (100 mM Tris ...
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bioRxiv - Immunology 2022Quote: ... into 293T cells in DMEM medium + 10% FCS using Fugene 6 (Promega) for pseudoviruses production ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with plasmids encoding fluorescently tagged proteins using FuGENE® HD Transfection Reagent (Promega), following manufacturer’s protocol and assayed 1-3 days after transfection ...
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bioRxiv - Molecular Biology 2023Quote: FLAG-tagged SPUD in vitro expression was done with Transcend Non-Radioactive Translation Detection System (Promega) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: lrl 1μg of N-terminally FLAG-tagged receptor and 1μg of F22 (Promega, Cat. no: E2301) (for GloSensor assay)
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 50 ng of plasmids encoding the HiBiT-tagged proteins using FuGENE HD (Promega). Cells were trypsinized (Gibco ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of GST-tagged effector proteins and 2 μl of FluoroTect™ GreenLys tRNA (Promega, USA) were gently mixed with High-Yield Wheat Germ Master Mix ...
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bioRxiv - Biochemistry 2021Quote: ... HEK-293 cells were transfected with FLAG-tagged receptor (3.5 μg) and luciferase-based 22F cAMP biosensor construct (3.5 μg) (Promega). 14–16 h post transfection ...
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bioRxiv - Cell Biology 2021Quote: ... Halo-tagged proteins were labeled by incubating cells with 200nM HaloTag Ligand conjugated to Janelia Fluor 646 (Promega) for 30 min before imaging ...
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bioRxiv - Biochemistry 2021Quote: ... selections were performed using biotinylated c-CDCP1-Fc captured on SA-coated magnetic beads (Promega). Prior to each selection ...
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bioRxiv - Bioengineering 2023Quote: ... selections were performed using biotinylated Fc fusion antigen captured on SA-coated magnetic beads (Promega). Prior to each selection ...
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bioRxiv - Plant Biology 2019Quote: ... HA- and GST-tagged proteins were synthesized in vitro using the TNT® Coupled Wheat Germ Extract Systems (Promega). For the pull-down assays ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with 2 μg of FLAG-tagged β2 or β1 adrenergic receptor DNA using Fugene 6 (Promega) and seeded at a density of ∼70,000 cells.ml−1 on 18 mm glass coverslips 4-8 hours after transfection.