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Citations for Promega :
1 - 50 of 8345 citations for Rat T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: The T Cell Activation Bioassay kit was purchased from Promega. The Jurkat-NFAT cells were thawed and cultured in RPMI (supplemented with 10% FBS and 5% pen/strep ...
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bioRxiv - Plant Biology 2019Quote: ... pGEM®-T kit from Promega were used ...
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bioRxiv - Cancer Biology 2021Quote: T Cell Activation Bioassay (Promega) was performed according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Polymerase chain reaction (PCR) was performed using GoTaq kit (Promega, Madison, WI) unless otherwise indicated.
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... Polymerase chain reaction (PCR) was performed using GoTaq kit (Promega, Madison, WI).
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bioRxiv - Biochemistry 2023Quote: ... Bovine alpha-2-hs-glycoprotein was isolated from plasma (Fetuin, Promega, V4961), and monocyte differentiation antigen CD14 was recombinantly expressed in CHO cells (R&D systems ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... or a pGEM®-T Easy Vector (pGEM®-T Easy Vector Systems Kit, Promega, USA) depending on the Taq-polymerase used for PCR (blunt vs ...
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bioRxiv - Biochemistry 2020Quote: ... The pGEM-T Vector kit was purchased from Promega. Acrylamide/bisacrylamide (29:1 ...
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bioRxiv - Plant Biology 2020Quote: ... and cloned into the pGEM T-Easy vector kit (Promega). Clones were fully sequenced and confirmed as the targeted sequence ...
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bioRxiv - Microbiology 2021Quote: ... and pGEM®-T vector kit (Promega, Madison, Wisconsin, USA), respectively ...
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bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
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bioRxiv - Genomics 2023Quote: ... The total cellular DNAs of the activated CD4+ T cells were isolated using a DNA extraction kit (Promega Wizard) for analysis.
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bioRxiv - Developmental Biology 2022Quote: ... or pGEM vector using pGEM-T easy vector system kit (Promega). Inserted sequences were excised by digestion with ClaI and inserted into the ClaI site of the replication-competent retroviral vector RCASBP(A) ...
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bioRxiv - Molecular Biology 2022Quote: ... Bands <2kb were cloned using the pGEM-T Easy kit (Promega) and bands >2kb were cloned using the Topo-XL2 PCR kit (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... We used the pGEM® T-Easy Vector System kit (Promega) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... and cloned into pGEM-T in JM109 competent cells (Promega). C ...
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bioRxiv - Immunology 2020Quote: The ability of T cells to lyse organoids was also measured using LDH-Glo™ Cytotoxicity Assay Kit (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: RNA from purified splenic CD8 T cells was reverse transcribed into cDNA using oligo-d(T) primers and M-MuLV reverse transcription (Promega). Real-time quantitative PCR was performed using SYBR DNA polymerase (Thermo Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... the full-length fragment of human PFKFB3-5 was amplified with primers PFKFB3-5 reverse and PFKFB3-5 forward and subcloned into the plasmid pGEM-T using the T/A Cloning Kit (Promega, Mannheim, Germany). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... Ca2+-dependent nuclear factor of activated T-cells (NFAT) (pGL4.30; Promega), or the MAPK signaling pathway (pGL4.33 ...
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bioRxiv - Immunology 2021Quote: CD8+ T cell cytotoxicity was assessed using a Cyto Tox 96® Non-radioactive Cytotoxicity Assay kit (Promega, Cat no. G1780) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... T cell cytotoxicity was assessed by quantification of LDH levels in the supernatants using the LDH-Glo cytotoxicity kit (Promega, J2380) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... and 1 μg of a plasmid expressing the VSV G glycoprotein using the FuGene HD reagent (Promega). Forty-eight hours later ...
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bioRxiv - Microbiology 2023Quote: ... and 0.25 μg vesicular stomatitis virus G glycoprotein expression plasmids using the Fugene 6 transfection reagent (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK cells were transfected with both heavy and light chain BCR constructs using FuGENE HD transfection reagent (Promega) in a 1:3 ratio ...
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bioRxiv - Molecular Biology 2022Quote: ... 7 and 10 were either cloned with the pGEM®-T kit (Promega) to assay individual sequences and/or sequenced by Illumina (Centre d’expertises et de services Genome Québec) ...
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bioRxiv - Microbiology 2021Quote: Peptides from cell-surface/biofilm-ECM-associated proteins were released via a Trypsin/Lys-C mix (Promega) at a final concentration of 50 ng µL-1 ...
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bioRxiv - Immunology 2021Quote: ... TCR- and CD3-encoding pMIG II vectors were used to transiently transduce HEK293T cells using FUGENE (Promega). Cells were harvested after 48h and assessed for CD3/TCR expression and CD1d-tetramer binding using flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... Confirmed heavy and light chain plasmid DNA were co-transfected in 293T cells (ATCC) using Fugene transfection reagent (Promega) in 24 well plates for preparing antibody supernatant for initial screening for their expression and antigen specificity as detailed in the following section ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Polymerase chain reactions were performed using 2 U Gotaq (Promega), 1X Gotaq buffer (Promega) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... or into the pGEM-T or pGEM-T Easy vector (Promega) and subsequently sequenced (Macrogen).
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bioRxiv - Plant Biology 2022Quote: ... The amplicons were subcloned using pGEM-T Easy Vector Systems Kit (Promega, Tokyo, Japan) or Zero Blunt PCR Cloning Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Each product was cloned using the pGEM®-T Easy vector kit (Promega Corp.) to derive 12 clones using the GeneJet plasmid purification kit (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 25,000 HEK293-T cells were seeded and transfected with FuGENE 6 (#E231A, Promega) and plasmid DNA at the carrier (µL):DNA (µg ...
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bioRxiv - Cell Biology 2021Quote: ... T cells were incubated for 72 hours prior to flow cytometry analysis or Cell Titer Glo (Promega) analysis.
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bioRxiv - Developmental Biology 2023Quote: ... or pGEM-T (Promega) vectors and eight positive colonies/gene transferred to 750 ul of ddH2O and heated to 90 C to liberate the plasmids ...
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bioRxiv - Genetics 2020Quote: ... then cloned using the pGEM T-Easy kit and manufacturer’s protocol (Promega, Madison, WI, USA). pGEM vector containing the desired PCR product was transformed into competent JM109 E.coli cells (Promega ...
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bioRxiv - Microbiology 2021Quote: ... The SK-N-SH cell surface proteins with attached TBEV virions were digested off with 1.25 μg trypsin (Promega) and the supernatant collected ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cell surface levels of HiBiT-tagged human S1PR1 were monitored using a Nano Glo HiBiT Extracellular Detection System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells that showed indels were then cloned into the pGEM-T Easy vector (Promega), and colonies were picked using blue/white screening then sequenced ...
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bioRxiv - Genetics 2021Quote: ... All other polymerase chain reactions (PCR) were performed using the GoTaq (Promega) enzyme system as per manufacturer instructions (95°C for 2 min ...
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bioRxiv - Pathology 2022Quote: ... The pGEM T-easy (Promega) based positive control plasmid of NNV was constructed from a 605 bp RNA2 fragment amplified from NNV-infected orange-spotted grouper (laboratory sample) ...
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bioRxiv - Microbiology 2019Quote: ... and pGEM-T Easy (Promega) were used ...
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bioRxiv - Microbiology 2022Quote: ... pGEM-T Easy vector (Promega) (supplementary data table 6) ...
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bioRxiv - Developmental Biology 2023Quote: ... cloned into pGEM-T (Promega) and used to synthesize digoxigenin-labeled probes by in vitro transcription using SP6 or T7 polymerases (Thermo-Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were cloned into pGEM-T Easy and transformed into DH5α competent cells (Promega). Plasmid DNA was purified using the Qiagen midiprep kit (Qiagne ...
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bioRxiv - Immunology 2024Quote: The cytotoxicity of CAR T cells was evaluated using a luciferase assay system (Promega, USA) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... which were subsequently ligated to pGEM-T Vector (pGEM-T Vector System I, Promega, USA), and transformed to DH5α competent cells (Z-Competent E ...
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bioRxiv - Cancer Biology 2019Quote: ... rat or cynomolgus ICOS as target cells were co-incubated with ADCC reporter cells expressing human FcγRIIIa (V158; Promega) at a 5:1 ratio ...