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Citations for Promega :
1 - 50 of 4410 citations for Rat High Mobility Group Protein B1 HMGB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Electrophoretic Mobility Shift Assays were performed using proteins expressed in the TNT T7 Coupled Reticulocyte Lysate System (Promega, WI), according to the manufactureŕs recommendation ...
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bioRxiv - Neuroscience 2022Quote: RNA was extracted from sections of frozen mouse brain cerebrum representing each treatment group (n = 6 per group) using the Maxwell 16 LEV simplyRNA Tissue Kit (Promega, Ipswich, MA, USA; AS1270). RNA was extracted from cultured cells using the Maxwell 16 Cell LEV Total RNA Purification Kit (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... and mouse cyclin B1 were generated using the Riboprobe in vitro Transcription Systems kit according to the manufacturer’s protocol (Promega). Anti-BrdU antibodies were conjugated with protein A-sepharose beads overnight at 4C (GE Healthcare) ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were expressed with TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega) and reactions were incubated for 12h at 25°C with 10µg pIX-HALO expression clone ...
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bioRxiv - Biochemistry 2021Quote: ... and the S30 T7 High-Yield Protein Expression System (Promega) were used to express proton-coupled folate transporter (PCFT ...
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bioRxiv - Biophysics 2020Quote: ... The construct expresses rat GR fused with HaloTag protein (Promega, Madison, WI, USA) in the C-terminal domain under the CMVd1 promoter ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Group two-choice assay: agarose (Promega) substrates were prepared as follows ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were co-expressed using TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) as per manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... to crosslink the silane groups and the amine group of halo-ligand (HaloTag amine O4 ligand, Promega) (Figure 3B) ...
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bioRxiv - Developmental Biology 2020Quote: TNT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) was used to perform in vitro translation assay ...
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bioRxiv - Molecular Biology 2023Quote: ... the TnT SP6 High-Yield Wheat Germ Protein Expression System (Promega) was used ...
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bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
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bioRxiv - Biochemistry 2019Quote: The linear dynamic range for measurement of complex peptide mixtures on the Lunatic was determined on a group of eight solutions prepared from a 1.0 μg/μl stock of a commercial yeast protein digest (Promega, V7461), dissolved in loading solvent (0.1% TFA in acetonitrile/water ...
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bioRxiv - Plant Biology 2021Quote: ... TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega L3260), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega, L3260) was used to generate Halo-tagged proteins starting with pIX-HALO plasmids as described previously (Mukherjee et al. ...
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bioRxiv - Plant Biology 2023Quote: ... the PhDEF and PhGLO1 proteins were produced with the TnT SP6 High-Yield Wheat Germ Protein Expression System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were expressed using TNT® SP6 High-Yield Wheat Germ Protein Expression System (Promega, Madison, Wisconsin, United States) with 2 µg Plasmid DNA as input ...
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bioRxiv - Microbiology 2021Quote: ... proteins were expressed by in vitro transcription/translation using the TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega) prior to incubation with the affinity resins ...
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bioRxiv - Plant Biology 2023Quote: ... These vectors were used for in vitro protein production using SP6 High-Yield Wheat Germ Protein Expression System (Promega L3260) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... EIL1-GST and GST proteins were synthesized using the TnT SP6 High-Yield Wheat Germ Protein Expression System (Promega, L3260), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting proteins with carbamidomethyl groups on their cysteines were digested with 300 ng of sequencing-grade porcine trypsin (Promega, Fitchburg, MA, USA) and injected on an Easy-nanoLC-1000 system coupled to a Q-Exactive+ mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... The reactions were performed using the S30 T7 High-Yield Protein Expression System (Promega). 3 μl of peptide solution were added to reaction mixtures to get a final concentration respectively of 32 μg/ml or 512 μg/ml in a final volume of 15 μL in nuclease-free PCR tubes ...
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bioRxiv - Plant Biology 2021Quote: ... The pSPUTK promoter allowed in vitro protein synthesis using the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... we extracted the genomic DNA from two samples of each group using the Wizard Genomic DNA purification kit (Promega, A1120). The DNA concentration was measured by the Qubit dsDNA Broad-Range Assay (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... n=4 vaginal) and rUTI (n=3 urine) cohorts belonging to B2 phylogenetic group was extracted (Wizard Genomic DNA Purification Kit, PROMEGA) and sequenced by Illumina NovaSeq 2×150 nt ...
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bioRxiv - Plant Biology 2024Quote: ... We further used the TNT SP6 High-Yield Wheat Germ Protein Expression System (L3260, Promega) to express the four TFs-HaloTag fusion protein ...
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bioRxiv - Genetics 2021Quote: ... and Pierce 660nm Protein Assay kit (Promega) was used for quantification ...
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bioRxiv - Plant Biology 2021Quote: ... United States The HY5 protein was expressed with TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega, Madison, Wisconsin, United States) using 2 μg Plasmid DNA per 50 μL expression reaction ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... AccuMAP™ Low pH Protein Digestion Kit (Promega) according to the manufactures protocol ...
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bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
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bioRxiv - Microbiology 2019Quote: Cell-free translated His-VqmA was produced using the S30 T7 High-Yield Protein Expression System (Promega) according to the manufacturer’s protocol with the following amendments ...
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bioRxiv - Cell Biology 2022Quote: ... IVT reactions were done with the TNT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: LDH activity and percentage cell cytotoxicity in rabbit lungs (n=3 per group per time point) were determined using the LDH-Glow cytotoxicity kit as per the manufacturer’s instructions (Promega Corporation, Madison, WI, USA). Luminescence was recorded using a GloMax plate reader ...
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bioRxiv - Microbiology 2021Quote: ... The amounts of luciferase within each group were measured utilizing luminometer (Promega) 48 hours post infection ...
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bioRxiv - Biochemistry 2021Quote: ... and S30 Premix Plus are components of the S30 T7 High-Yield Protein Expression System purchased from Promega. The pEXP5-PCFT plasmid is the PCFT construct shown in Fig ...
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bioRxiv - Biochemistry 2022Quote: ... laevis TRAIP that was produced in the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... flag-tagged MED25 protein and CFP-tagged RAP2.2 and RAP2.12 proteins (Schmidt et al., 2018) were expressed using TNT SP6 High Yield Wheat Germ (Promega) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... cells were grown on glass coverslips for 24 h and then transfected with both mCherry-lamin B1-10 and GFP-connexin-43 using ViaFect (Promega). The mCherry-lamin B1-10 was a gift from Michael Davidson (Addgene plasmid #55069) ...
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bioRxiv - Cell Biology 2020Quote: ... securin-CFP was in vitro translated using the TNT SP6 high-yield wheat germ protein expression system (Promega, #L3261). Three or 4 µL of in vitro translated securin-CFP was added to 50 or 80 µL extract ...
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bioRxiv - Molecular Biology 2020Quote: ... ZmCLA4 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260) (Promega), and then purified using Magne HaloTag Beads (G7281 ...
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bioRxiv - Plant Biology 2022Quote: ... ZmEREB57 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260) (Promega), and then purified using Magne HaloTag Beads (G7281 ...
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bioRxiv - Cell Biology 2020Quote: ... The protein concentration was estimated using a BCA kit (Promega). The blots were blocked using 5% NFDM (Santa Cruz ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using Bradford Assay Kit (Promega, USA) and equal amounts of proteins were denatured ...
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bioRxiv - Immunology 2020Quote: ... and cDNA synthesized from 1 µg total RNA (High Capacity cDNA Reverse Transcription Kit, Promega). Quantitative RT-PCR was performed using FluoCycle II SYBR Master Mix (EuroClone ...
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bioRxiv - Cell Biology 2022Quote: ... Baseline fluorescence was measured in an ELISA reader (Promega) following an incubation of 30 min at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: In vitro translation (IVT) with 500 ng of mRNA was performed using the TnT SP6 High-Yield Protein Expression System (Promega) to synthesize tagged proteins ...
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bioRxiv - Plant Biology 2021Quote: ... Halo fusion proteins were produced from 1.5 µg plasmid DNA in a 15 µL reaction using TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega). Protein expression was confirmed by Western blot with Anti-HaloTag monoclonal Ab (1:2000 ...
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bioRxiv - Plant Biology 2022Quote: ... The Halo fusion proteins were produced using 1.2 μg plasmid DNA of the respective pIX-HALO construct in a volume of 15 μl using the TNT SP6 High-Yield Wheat Germ Protein Expression System (L3260, Promega). Protein expression was confirmed by Western blot with Anti-Halo Tag monoclonal antibody (1:2000 ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were produced using 5 µg of plasmids in 25 µL in vitro translation reaction of TnT SP6 High-Yield Wheat Germ Protein Expression System (Promega). For the OSH15-RI complex ...
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bioRxiv - Genomics 2023Quote: ... Protein was precipitated by the addition of 200 μl Protein Precipitation Solution (Promega Wizard HMW DNA Extraction Kit A2920). Using a wide bore tip the samples were mixed by drawing up contents from the bottom of the tube and then expelled on the side of the tube 5 times ...