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Citations for Promega :
1 - 50 of 4133 citations for Rat Antigen peptide transporter 2 TAP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Rat Gβ1 was cloned with a C-terminal SmBiT34 (peptide 86 or HiBiT, Promega) connected with a 15AA polypeptide linker ...
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bioRxiv - Biochemistry 2022Quote: ... Rat Gβ1 with an N-terminal MHHHHHHSSGLVPRGSHMASHHHHHHHHHH-tag (His16) was fused with the SmBiT subunit (peptide 86, Promega)20 via a 15 amino acid (GSSGGGGSGGGGSSG ...
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bioRxiv - Biochemistry 2023Quote: ... peptides were collected as described in ProteaseMax application note (Promega, TB373, Rev. 2/15), and purified using Pierce C18 Spin Tips (Thermo Fisher PI84850) ...
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bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 25% confluent wells were transfected with 2 µg plasmid vector expressing SV40 antigen using Fugene Transfection Reagent (Promega) as described by manufacturer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells expressing the transporters were additionally transfected with a plasmid coding for NanoLuc (N1411, Promega, Madison, WI) and selected with 40 μg/ml hygromycin B (InvivoGen ...
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bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
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bioRxiv - Biochemistry 2020Quote: ... CellTiter-Glo 2 kit (Promega) using manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... SmBiT34 (peptide 86, Promega) subunit was added to the C terminus of Rat Gβ1 with a 15AA polypeptide linker between them ...
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bioRxiv - Cancer Biology 2022Quote: ... Biotinylated antigens were immobilized using streptavidin-coated magnetic beads (Promega). In each round ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... HaloTag-GST was used as a negative control antigen (Promega).
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in a 6-well plate and transfected with 10 µg of linearised plasmid expressing the SV40 Large T antigen using the FuGene Transfection Kit (Promega, #E2691) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Purified EDIII antigens were biotinylated using HaloTag PEG biotin ligand (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
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bioRxiv - Cell Biology 2022Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer with Azure Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer with Azure Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... of miR-3594-5p was amplified from rat brain cDNA and cloned downstream of the Renilla luciferase reporter gene into the psiCHECK-2 vector (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... viable cells were quantified in a FLUOstar OPTIMA ELISA reader (550/590 nm) about 2 h after CellTiter-Blue staining (Promega, Cat No. G8081). Mean values +/-standard deviations (SD ...
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bioRxiv - Biophysics 2021Quote: ... C1 peptide was from Promega (Madison, WI). Lyophilized ATP was from Expedeon (Cambridge ...
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bioRxiv - Systems Biology 2020Quote: ... and an external reference peptide mix (Promega) was spiked into the sample at the concentration of 0.5 pmol/µl ...
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bioRxiv - Biochemistry 2024Quote: ... a K562 human peptide digest standard (Promega) and E.coli peptide standard (Waters ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were digested using trypsin/LysC (Promega) overnight with head-over-head mixing ...
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bioRxiv - Cell Biology 2019Quote: ... The following primary antibodies against the antigens were used: Tuj-1 (1:300, Promega), Synaptophysin (1:400 ...
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bioRxiv - Plant Biology 2020Quote: ... Antigen on the membrane was visualized with horseradish peroxidase-coupled anti-rabbit IgG (Promega) for VHA-a1 and VHA-a3 ...
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bioRxiv - Immunology 2021Quote: ... RBD and NTD antigens were site-specifically biotinylated using Halotag PEG biotin ligand (Promega), following the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... Antigen retrieval was performed by incubation with 20 μg/mL proteinase K (Promega, USA) for 20 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Baseline fluorescence was measured in an ELISA reader (Promega) following an incubation of 30 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... alkylated and digested into peptides with trypsin (Promega). The resulting peptides were desalted and concentrated before mass spectrometry by the STAGE-TIP method using a C18 resin disk (3M Empore) ...
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bioRxiv - Zoology 2020Quote: After digestion into short peptides by trypsin (Promega), the samples were analyzed using a DiNa nanoLC system (KYA Technologies ...
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bioRxiv - Biochemistry 2022Quote: LC-MS/MS Peptide Reference Mix (V7491, Promega) in HeLa digest (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were eluted by on-bead trypsin (Promega) digest and acidified for MS analysis ...
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bioRxiv - Immunology 2022Quote: ... alkylated and digested into peptides using trypsin (Promega). Peptides were desalted and concentrated using Empore-C18 StageTips and eluted with 0.5% (v/v ...
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bioRxiv - Molecular Biology 2019Quote: A commercial kit (HDAC-Glo™ 2 Assays, Promega, G9590) was used to measure HDAC2 activity ...
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bioRxiv - Neuroscience 2020Quote: ... using the GoTaq® 2-step RT-qPCR kit (Promega). Quantitative PCR runs were performed on a 7900HT Fast Real-Time PCR System (Applied Biosystems Inc. ...
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bioRxiv - Bioengineering 2023Quote: ... selections were performed using biotinylated Fc fusion antigen captured on SA-coated magnetic beads (Promega). Prior to each selection ...
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bioRxiv - Cell Biology 2020Quote: ... st-Ht31 inhibitor peptide was from Promega (Madison, WI).
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bioRxiv - Biophysics 2021Quote: ... 49 (peptide 86 and also named as HiBiT, Promega) with a 15-amino acid (15AA ...
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bioRxiv - Plant Biology 2023Quote: ... Peptides were digested using sequencing grade trypsin (V5113; Promega) diluted in 50 mM Triethylammonium bicarbonate buffer pH 8.5 (T7408 ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were digested using sequencing grade trypsin (V5113; Promega) diluted in 50 mM Triethylammonium bicarbonate buffer pH 8.5 (T7408 ...
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bioRxiv - Cell Biology 2023Quote: ... Presence of rat cells in myoblast culture/muscle lysate was assessed by PCR for rat dystrophin using primers Rat Dmd i22-i23.F/ Rat Dmd i22-i23.R and GoTaq G2 Hot Start Green Master Mix (M7423, Promega) via the following program ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins/peptides were further digested with trypsin (Trypsin Gold, Promega) at 37 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Dual Luciferase assay kit and PsiCHECKTM-2 vector were purchased from Promega. Oligonucleotides were synthesized and obtained from Eurofins Scientific ...
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bioRxiv - Physiology 2020Quote: ... The transferase dUTP Nick End Labeling (TUNEL) assay was performed on cryosections of rat eyes using the TUNEL apoptosis detection kit (DeadEnd Fluorometric TUNEL System; Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... ELISA plates were read on a GloMax plate reader (Promega, Wisconsin, US). Data were converted from concentration to total mass based on the volume of media/pellet ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were released by digestion with PNGase F (Promega, Madison, WI) overnight at 37 °C with vortexing ...
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bioRxiv - Systems Biology 2022Quote: ... peptides were separated were spotted on streptavidin-conjugated membranes (Promega #V2861) where they tightly associated via their C-terminal biotinylations ...
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bioRxiv - Biochemistry 2021Quote: ... A 15-amino acid linker and SmBiT subunit (peptide 86, Promega) were attached to the C-terminus of rat Gβ1 ...
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bioRxiv - Developmental Biology 2022Quote: ... Purified protein was then digested into peptides using Trypsin (Promega, V5111) and desalted using Pierce C18 Spin Columns (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... and then the proteins were digested to peptides using trypsin (Promega) at a trypsin/protein ratio of 1:50 (w/w ...