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Citations for Promega :
1 - 50 of 9001 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Protein-protein interaction was analyzed using the NanoBit Protein:Protein Interaction (PPI) System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: A NanoBiT protein:protein interaction assay (Promega) was used to assess the interaction between ZMPSTE24 and IFITM3 ...
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bioRxiv - Plant Biology 2021Quote: ... Protein preparation and luciferase– substrate interaction were conducted with a Luciferase Assay Report Kit (Promega). Luciferase activity was measured by a microplate reader (Thermo Scientific Varioskan Flash) ...
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bioRxiv - Molecular Biology 2019Quote: PERP protein-protein interactions were isolated using the HaloTag Mammalian Pull-Down System (Promega) and standard protocol ...
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bioRxiv - Cancer Biology 2022Quote: NanoBRET Protein:Protein Interaction assay was performed following the manufacturer’s conditions (Promega, N1662). 0.5×106 HEK293T cells were plated the day before transfection in a 12 well plate ...
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bioRxiv - Plant Biology 2023Quote: ... The selected interactions were verified by quantitative β-galactosidase assays using Beta-Glo Assay System (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Microbiology 2020Quote: The interactions between host protein and RHDV nonstructural proteins were evaluated using a CheckMate Mammalian Two-Hybrid System (Promega). The proteins expressed from the pACT vector recombinant plasmid acted as prey proteins ...
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A Phytochrome B-PIF4-MYC2 Module Tunes Secondary Cell Wall Thickening in Response to Shaded LightingbioRxiv - Plant Biology 2021Quote: The interaction of MYC2 and PIF4 activity was assayed using a dual-LUC reporter assay system (Promega) through Arabidopsis protoplast transfection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Stimulation of P-gP ATPase activity was utilized to indicate the interaction between peptides and P-gP using the P-gP-Glo Assay System (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... A BCA protein assay (Promega) was used to determine protein concentration ...
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bioRxiv - Genetics 2021Quote: ... and Pierce 660nm Protein Assay kit (Promega) was used for quantification ...
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bioRxiv - Biophysics 2021Quote: Some protein kinase assays were performed using the ADP-Glo™ assay kit (Promega), which measures the generation of ADP in a kinase reaction ...
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bioRxiv - Cell Biology 2021Quote: For quantitative analysis of Atg32-Atg11 interactions using NanoLuc Binary Technology (NanoBiT, Promega), Atg32 fused to 3 copies of GFP and Large BiT (LgBiT ...
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bioRxiv - Plant Biology 2023Quote: ... and the total protein contents were quantified using BCA Protein Assay Reagent (Promega Corporation). The supernatants were stored at –80 ℃ until subsequent analysis.
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using Bradford Assay Kit (Promega, USA) and equal amounts of proteins were denatured ...
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bioRxiv - Molecular Biology 2023Quote: ... Both DNA and RNA luminescence assays were performed using the Dual-Glo Luciferase assay system (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The estimation of protein concentration was done using BCA protein assay (Promega #PI-23222, PI-23224). Samples were diluted using 1X sample buffer (4X stock ...
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bioRxiv - Cancer Biology 2022Quote: ... The estimation of protein concentration was done using BCA protein assay (Promega #PI-23222, PI-23224). Samples were diluted using 1 X sample buffer (4 X stock ...
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bioRxiv - Cell Biology 2022Quote: ... The estimation of protein concentration was done using BCA protein assay (Promega #PI-23222, PI-23224). Samples were diluted using 1X sample buffer (4X stock ...
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bioRxiv - Immunology 2019Quote: ... DNA encoding SmBit and LgBit for the NanoBiT assay (Promega) were inserted after the attR2 recombination site in a modified pLEX_307 vector (immediately after the EcoRV site) ...
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bioRxiv - Genomics 2023Quote: ... We added Nano-Glo Luciferase Assay Substrate and LgBiT protein (Promega) and imaged using the ImageQuant LAS4000 Imager.
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bioRxiv - Plant Biology 2023Quote: ... 1 μg protein was used for dual luciferase transactivation assay using Dual-Luciferase® Reporter Assay System (Promega) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: PKA activity (PepTag® Non-Radioactive Protein Kinase Assay kit V5340, Promega) was carried out according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Clarified lysates were quantified using the Pierce TM BCA protein assay (Promega) and boiled in 1x sample buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2019Quote: ... Luciferase assays (Firefly and Renilla) were done on the protein extracts according to manufacturer instructions (Dual-Luciferase Reporter Assay System, Promega). The signal was normalized to the amount of protein in the sample as determined by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... before analysis of luciferase protein levels using Dual-Glo luciferase assay system (Promega) and an EnSpire multimode plate reader (Perkin-Elmer).
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bioRxiv - Microbiology 2020Quote: ... Fragmented nuclear DNA was detected by DeadEnd Fluorometric TUNEL assay (Promega, Madison, WI) that coupled fluorescein-12-dUTP to the 3’-OH ends of the DNA ...
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bioRxiv - Genomics 2023Quote: ... DNA and cDNA concentrations were determined using a Quantifluor® Assay (Promega®). Samples were diluted to 200 pg/μL or 100 pg/μL for Nextera XT (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... The assay was validated using Taq DNA polymerase from two different manufacturers (Promega Gotaq Flexi DNA polymerase or Invitrogen Taq DNA polymerase ...
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bioRxiv - Genetics 2021Quote: The in vitro protein translations were performed using the TNT-assay (L4610) from Promega according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Half the lysate was used to quantify luciferase protein (Promega Luciferase Assay System #E1501) using a Spectramax L luminometer with reagent injectors in technical triplicate ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-dependent interactions were assessed by treating nuclear extracts with 1 U/μl RNasin® Ribonuclease Inhibitors (Promega, # N2615) as control or 1 mg/ml of RNase A (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... the DNA-coated beads were added to the interaction mixture (BB 1x : 20mM HEPES pH7.9, 100mM KCl, 5mM, 10% Glycerol; supplemented with 160µg acetylated-BSA (Promega R396A), 1.44nmol ds oligoB ...
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bioRxiv - Genomics 2023Quote: ... Protein was precipitated by the addition of 200 μl Protein Precipitation Solution (Promega Wizard HMW DNA Extraction Kit A2920). Using a wide bore tip the samples were mixed by drawing up contents from the bottom of the tube and then expelled on the side of the tube 5 times ...
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bioRxiv - Bioengineering 2021Quote: ... The amount of plasmid was determined using Picogreen DNA assay (#E2670, Promega, Madison, WI) following the manufacturers’ instructions.
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bioRxiv - Biochemistry 2024Quote: The assays were conducted using the DNA-PK Kinase Enzyme System (Cat # V4106, Promega), following the provided instructions ...
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bioRxiv - Biochemistry 2022Quote: ... in which the interaction between the Gα subunit and the Gγ subunit was monitored by the NanoBiT-based enzyme complementation system (Promega). Specifically ...
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bioRxiv - Cell Biology 2023Quote: Protein-protein interactions between Mkl1/2 and Foxo1/3/4 or glucocorticoid receptors were also measured using NanoBiT PPI Starter Systems (Promega). 293FT cells were seeded in 96-well white wall microplates and co-transfected with the LgBiT and SmBiT plasmids ...
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bioRxiv - Plant Biology 2022Quote: ... Eluc activity and protein concentration in supernatant were measured using the Luciferase Assay System (Promega) and the Pierce 660 nm Protein Assay Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was measured using Pierce™ BCA Protein Assay Kit and GloMax® Explorer Multimode Microplate Reader (Promega, Madison, WI, USA). Expression of OAT1 ...
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bioRxiv - Molecular Biology 2019Quote: ... Activity was normalized to total protein following quantification using the Dual Luciferase Reporter Assay System (Promega).
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bioRxiv - Molecular Biology 2020Quote: In vitro transcription/translation assays were carried out in a S30 T7 HighYield Protein Expression System (Promega). The full lenght Hrnpk cDNA template was cloned from total RNA obtained from GH4C1 cells and transferred to a pET28a Escherichia coli T7 expression vector (Novagen ...
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bioRxiv - Plant Biology 2020Quote: ... Protein for MUG and LUC assays was extracted on ice in 1x passive lysis buffer (PLB: Promega). MUG assays were performed by adding 40μl of protein extract to 100μl of MUG assay buffer (2mM MUG ...
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bioRxiv - Cell Biology 2023Quote: ... CTPS activity of purified CTPS1 and CTPS2 proteins was then analyzed using ADP Glo Kinase assay (Promega). CTPS1 and CTPS2 were diluted in assay buffer (50 mM Trizma ...
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bioRxiv - Bioengineering 2023Quote: ... 2.25 ng/uL Single-Stranded DNA Binding Protein (Sigma, cat# S3917 OR Promega, cat# M301A). 12.5 uL of master mix was mixed with 12.5 uL of 2x input oligonucleotide ...
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bioRxiv - Microbiology 2021Quote: ATPase activity of the purified proteins was determined using two kits – Enliten ATPase assay (FF2000, Promega, Moscow, Russia) and malachite green phosphate assay (MAK307 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were extracted using 100 μL of Passive Lysis Buffer from Dual-Luciferase reporter assay systems (Promega, E1980) according to manufacturer’s instruction and assayed using FLUOstar Omega (BMG Labtech) ...
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bioRxiv - Plant Biology 2020Quote: ... LUC activity was measured with 20 μl of protein sample and 100 μl of LUC assay reagent (Promega). Activation from the DHS was calculated as (LUC luminescence/rate of MUG accumulation ...
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bioRxiv - Plant Biology 2023Quote: ... LUC activity was measured with 20 mL of protein sample and 100 mL of LUC assay reagent (Promega). Promoter activation was calculated as (rate of MUG accumulation / LUC luminescence ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: ... Luciferase assay (Promega Dual Luciferase assay) was performed 24 hours post transfection as per the instructions in the kit ...