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Citations for Promega :
1 - 50 of 273 citations for PD L1 Human HEK293 mFc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: The PD-1/PD-L1 immune checkpoint bioassay (PD-1/PD-L1 Bioassay, Promega) was performed according to the manufacturer’s manual ...
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bioRxiv - Cancer Biology 2022Quote: ... PD-L1 neutralization was done in a PD-1/PD-L1 Blockade Bioassay (J1250, Promega).
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bioRxiv - Molecular Biology 2023Quote: ... to the 1x PBS. PD-1/PD-L1 blockade bioassay (cat. J1250) was purchased from Promega.
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bioRxiv - Biophysics 2023Quote: The activity of the masked antagonists was analyzed using a PD-1/PD-L1 Blockade Bioassay (Promega, J1250) according to a modified manufacturer’s protocol as described previously(32) ...
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bioRxiv - Cancer Biology 2022Quote: ... Human 26S proteasome purified from HEK293 cells was purchased from Promega. The proteasome chaperoning reaction tests were carried out as follows ...
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bioRxiv - Cancer Biology 2019Quote: CHO K-1 cells overexpressing hPD-L1 and the recombinant TCR ligand (hPD-L1 Antigen Presenting Cells, hPD-L1 aAPCs, Promega) and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE ...
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bioRxiv - Genomics 2022Quote: ... cells were transfected with 1μg L1-mCherry or L1-mCherry_RT-plasmid per well using 3μL FuGENE HD transfection reagent (Promega, Cat#: E2311) and 97μL Opti-MEM (Life Technologies ...
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bioRxiv - Cancer Biology 2019Quote: The activity of the inhibitors of hPD-1/hPD-L1 immune checkpoint was examined using the hPD-1/hPD-L1 Blockade Bioassay (Promega), according to the manufacturer’s instructions ...
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A rare variant on a common risk haplotype of HFE causes increased risk of hereditary hemochromatosisbioRxiv - Genetics 2019Quote: ... or HEK293 cells using Fugene 6 (Promega) following manufacturer’s instructions and studied 48-72 hours post-transfection.
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bioRxiv - Developmental Biology 2022Quote: ... and Klf4 vectors were co-transfected with pCL-Eco (Addgene ID 12371)149 in HEK293 cells with FuGENE6 (Promega) using low volume transfection protocol (Steffen et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were transfected with Fugene 6 (Promega). All other cell lines were transfected using Lipofectamine 2000 (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... into HEK293 using FuGENE 6 transfection reagent (Promega). Culture supernatant containing lentiviral particles was harvested after 24-48h incubation and passed through a 0.45 μm filter ...
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bioRxiv - Genomics 2022Quote: ... Each L1 was then cloned into pGEMT Easy Vector (Promega, Cat#: A1360). Ligations were incubated overnight at 4°C ...
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bioRxiv - Genetics 2022Quote: ... Full-length L1 elements were then cloned into pGEMT Easy Vector (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: HEK293 autophagy reporter cells (HiBiT-HaloTag-LC3, Promega #GA1040) were grown in in DMEM (ThermoFisher #31053-028 ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 were transiently transfected using Fugene HD (Promega, U.K.) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... HEK293 cells were transiently transfected using Fugene 6 (Promega) with GFP-Tnf 3’UTR reporter constructs and a pGL3-mCherry control construct ...
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bioRxiv - Plant Biology 2020Quote: ... The pEN-L1-AG-L2 vector was digested by EcoRV and ApaI (Promega) and purified from gel ...
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bioRxiv - Molecular Biology 2022Quote: HEK293 cells cotransfected with pFN21A HaloTag CMV Flexi vector (Promega) expressing PALLD and pNLF1-N [CMV Hygro] or pNLF1-C [CMV Hygro] vector (Promaga ...
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bioRxiv - Cell Biology 2021Quote: HEK293 stably expressing pcDNA3.1(+)_SSF-GCGR cells transfected with GloSensor20F (Promega) were lifted and resuspended in imaging media (DMEM without phenol red supplemented with 30 mM HEPES ...
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bioRxiv - Systems Biology 2021Quote: ... HEK293 cells were co-transfected using Fugene 6 transfection reagent (Promega) with MAC-tag (600ng ...
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bioRxiv - Genetics 2022Quote: ... cells were transfected with L1 reporter constructs using 4 μl FuGENE HD transfection reagent (Promega), 96 μl Opti-MEM (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: HEK293 cells were rinsed and lysed with Passive Lysis Buffer (PBL, Promega), protein concentrations determined using the DC protein assay (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... retroviral plasmid was transfected to HEK293 cells using FuGENE 6 (Promega, E2692). Two days after transfection ...
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bioRxiv - Molecular Biology 2022Quote: HCT116 cells were transfected using JetPrime(Polyplus) and HEK293 using Fugene (Promega) using a standard protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plasmid DNAs were transfected into HEK293 cells with ViaFect Transfection Reagent (Promega) according to manufacturer’s instruction.
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bioRxiv - Physiology 2023Quote: ... whereas HEK293 cells stably expressing a luminescent cAMP GloSensor (GS-293) (Promega) were previously created in our lab 25 ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with L1 reporter construct using FuGENE HD Transfection Reagent (Promega, prod number E2311) following the standard protocol ...
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bioRxiv - Genomics 2022Quote: ... cells were transfected with L1 reporter constructs using 4μL FuGENE HD transfection reagent (Promega, Cat#: E2311) 96μL Opti-MEM (Life Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... HEK293 cells were transfected in the 24 well plates using Fugene HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Retrovirus was produced through transfection of HEK293 cells using FuGENE 6 (Promega, E2692). Two days after transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... 25,000 HEK293-T cells were seeded and transfected with FuGENE 6 (#E231A, Promega) and plasmid DNA at the carrier (µL):DNA (µg ...
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bioRxiv - Neuroscience 2019Quote: ... genomic DNA from puromycin selected cells and wild-type HEK293 cells was extracted (Promega Wizard Genomic DNA Purification Kit #A1120 ...
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bioRxiv - Neuroscience 2022Quote: HEK293 cells (ATCC, Manassas, VA) stably expressing both D3R and GloSensor 22F-cAMP (Promega) were created for these experiments ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... HEK293 cells were transfected with the VHL-NanoLuc fusion constructs using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: HEK293-µOR cells were transfected with a plasmid encoding pGloSensor-20F cAMP reporter (Promega). Cells were harvested 24 h post transfection and resuspended at a 1.5x10^6 live cells/ml in assay media (DMEM without phenol red or FluoroBrite DMEM ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were transfected with 15 μg of an L1-expressing plasmid (pJM101/L1.3, pJM101/L1.3FLAG or pALAF008) using 45 μL of FuGENE HD (Promega) in 1,500 μL of Opti-MEM ...
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bioRxiv - Genomics 2023Quote: ... cells were transfected with 1µg L1 plasmid per well using 3µL FuGENE HD transfection reagent (Promega, Cat# E2311) and 97µL Opti-MEM (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Stable 3T3-L1 cell lines were generated in a similar manner using the transfection reagent Viafect (E4981; Promega), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: The intracellular NAD+ in 3T3-L1 cells or ASCs was measured using the NAD+/NADH-Glo assay kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells were treated for 8 hours with either 0.1 µmoles/L Coumermycin A1 (Promega) or an equivalent volume of DMSO ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were transfected using FuGeneHD transfection reagent according to manufacturer’s protocol (Promega, Cat # E2311). 48 hours post-transfection ...
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bioRxiv - Microbiology 2020Quote: ... HEK293 cells were transfected using FuGeneHD transfection reagent according to manufacturer’s protocol (Promega, Cat # E2311). 48 hours post-transfection ...
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bioRxiv - Biochemistry 2019Quote: HEK293 was used for transfection of plasmids with FuGENE HD Transfection reagent (Promega, Tokyo, Japan). RIPA buffer was used for obtaining proteins from whole cells ...
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bioRxiv - Immunology 2020Quote: ... expression constructs were transfected into the HEK293 EBNA cells using FuGENE HD transfection reagent (Promega). After selection with puromycin ...
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bioRxiv - Neuroscience 2023Quote: Plasmid constructs were acutely transfected into HEK293 cells using Viafect reagent (E4981; Promega, Madison, WI), following the manufacture’s protocol ...
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bioRxiv - Genetics 2021Quote: Pooled DNA extractions (minimum 90 adult mosquitoes or 3500 L1 larvae) were performed using the Wizard Genomic DNA Purification kit (Promega). A 349 bp locus containing the predicted on-target cleavage site was amplified with primers containing Illumina Nextera Transposase adapters (underlined) ...
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bioRxiv - Cancer Biology 2019Quote: ... HEK293 or MIA PaCa-2 cells were transfected with different AGO2 constructs using Fugene HD (Promega) or Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: HEK293 T cells were co-transfected with the target receptor construct and GloSensor cAMP reporter (Promega) in DMEM supplemented with 10% FBS for overnight incubation ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified BAC and plasmid DNA were transfected into HEK293 cells with FuGene HD transfection reagent (Promega) in a 1:3 DNA:FuGene HD ratio ...