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Citations for Promega :
1 - 50 of 5141 citations for Mouse Ras Related Protein M Ras MRAS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Total RNA from MB cells treated with RA for 48 h was extracted using the SV Total RNA Isolation System kit (Promega, Madison, USA), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: We tested the functionality of our purified NF1 by evaluating its RAS-GAP activity through the GTPase-Glo™ assay (Promega), a bioluminescent assay that quantitates the amount of GTP remaining after a GTPase reaction ...
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bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
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bioRxiv - Genetics 2024Quote: ... Proteins were immediately solubilized in 8 M urea and tryptic (Promega) digestion was performed overnight at 37°C with shaking followed by desalting ...
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bioRxiv - Immunology 2023Quote: ... cDNA was synthesized with M-MLV reverse transcriptase Kit (Promega) and diluted 1/5 before further application ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were digested in 1 M urea with modified trypsin (Promega, Madison, WI) at a 1:50 enzyme-to-substrate ratio ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein pellets were resuspended in 8 M urea buffer combined with protease inhibitor (Promega) and purified using the chloroform-methanol precipitation method (Wessel & Flügge ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized with the M-MLV reverse-transcriptase kit (Promega) and diluted 1 in 5 before further application ...
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bioRxiv - Genetics 2019Quote: ... and cDNA synthesis (M-MLV RT kit, Promega Corporation, cat. #M170A) were performed according to manufacturer’s specifications [21] ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized with the M-MLV reverse-transcriptase kit (Promega) and diluted 1 in 5 before further application ...
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bioRxiv - Physiology 2022Quote: ... Reverse transcription (RT) was performed with M-MLV enzyme kit (Promega) and qPCR was conducted with the Syber GreenER qPCR Super Mix Universal (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... Complementary DNA (cDNA) was synthesized using M-MLV retrotranscriptase kit (M1701, Promega) and random primers (B070-40 ...
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bioRxiv - Systems Biology 2022Quote: ... The proteins were diluted to 0.1 M ammonium bicarbonate and digested overnight with Trypsin (Promega, V5113) at 37°C with a protein-to-enzyme ratio of 50:1 ...
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bioRxiv - Biophysics 2020Quote: ... Trypsin was added to 1:50 (m/m) (Promega) and incubated overnight at 37°C with 600 rpm shaking on a thermomixer ...
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bioRxiv - Synthetic Biology 2019Quote: Iron-related cytotoxicity was monitored with a Luciferase-based viability assay (RealTime-Glo™ MT Cell Viability Assay, Promega) according to the protocol of the manufacturer in 96-well plate format as an endpoint measurement ...
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bioRxiv - Plant Biology 2023Quote: ... Protein marker was purchased from Fermentas (Beijing) and M-MLV reverse transcriptase was purchased from Promega (Beijing). Homologous recombinant kit was purchased from Clone Smarter (Beijing) ...
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bioRxiv - Evolutionary Biology 2020Quote: The RNA was transcribed to cDNA using M-MLV reverse transcription kit (Promega) following the manufacturer’s recommendations using a defined amount of RNA (1μg for bees and 50 ng for mites ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were further diluted to a final urea concentration of 2 M and proteins digested with trypsin (Promega) (1/100 ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was performed in triplicate using M-MLV Reverse Transcriptase Kit (Promega, USA) according to the manufacturer’s instructions with modifications ...
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bioRxiv - Immunology 2023Quote: ... 2 μg RNA was used to synthesize first-strand cDNA using Moloney mouse leukemia virus (M-MLV) (Promega). The amplification of specific PCR products was detected using SYBR Premix Ex TaqTM II (Vazyme) ...
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bioRxiv - Genetics 2021Quote: ... and Pierce 660nm Protein Assay kit (Promega) was used for quantification ...
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bioRxiv - Biophysics 2022Quote: ... Then the samples were diluted to 1 M urea with 50 mM (NH4)HCO3 (AB) and trypsinized (1:50 m/m, Promega) at 37 °C (overnight ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were precipitated with 0.1 M ammonium acetate in 100% methanol and the protein pellets were further digested with sequencing-grade trypsin (Promega). For the analysis involving the rpsAΔ6 mutant missing the last C-terminal OB fold domain of protein S1 ...
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bioRxiv - Biochemistry 2019Quote: ... All samples were further diluted with 20 mM HEPES pH 8.0 to a final urea concentration of 2 M and proteins were digested with 5 μg trypsin (V5111, Promega) (1/100 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we dissolved 10 μg of protein in 10 μl 10 M urea containing 0.1% ProteasMax (Promega. Madison, WI, USA). Cysteine residues were reduced with 5 mM DTT (30 min at 50 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were further diluted with 50 mM Tris pH 7.9 pH 8.0 to a final urea concentration of 2 M and proteins were digested with 5 µg trypsin (Promega) (1/100 ...
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bioRxiv - Cell Biology 2023Quote: ... urea was diluted to 1 M and proteins digested overnight with modified sequencing grade trypsin (Promega, Madison, WI, USA) at a 1:50 enzyme:protein ratio ...
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bioRxiv - Immunology 2021Quote: ... M-MLV (Promega, M1701) in the presence of RNase inhibitor (Promega ...
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bioRxiv - Microbiology 2023Quote: ... M-MLV (Promega, #M1701); Random primers (Invitrogen™ ...
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bioRxiv - Biochemistry 2023Quote: M-MLV buffer (Promega), 1 μL 0.1M DTT ...
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bioRxiv - Developmental Biology 2021Quote: ... Then RNA samples were reverse transcribed to cDNA by M-MLV reverse transcription kit (Promega). The levels of relevant mRNAs were quantitated by real-time PCR using One Step SYBR GREEN RT-PCR Kit (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... and then cDNA was synthesized using M-MLV reverse transcription kit (Promega, Madison, WI, USA). Relative expression was assessed by qPCR with SyberGreen II mix (Agilent Technologies Thermocycler ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by reverse transcription (RT) using M-MLV reverse transcription kit (Promega, Madison, WI, USA). Blank qPCR Master Mix kit (EURx ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... AccuMAP™ Low pH Protein Digestion Kit (Promega) according to the manufactures protocol ...
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bioRxiv - Pathology 2020Quote: ... Sample pH was adjusted to 7.5 with 0.05 M TEAB and proteins were digested overnight at 37 °C with sequencing-grade trypsin (Promega, Madison, WI) in 1:30 (w/w ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were re-solubilized in 6 M urea buffer followed by reduction and alkylation before digestion with trypsin (Promega) at 37°C for 18 hr ...
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bioRxiv - Biochemistry 2019Quote: ... in vitro translated hnRNP M protein fragments (5 μl) produced by rabbit reticulocyte lysate using TNT Quick Coupled Transcription/Translation Systems (Promega). Proteins were incubated at 4°C for 2 hours in the binding buffer above ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was diluted 1:7 with 0.1 M NH4HCO3 before overnight digestion of proteins with trypsin (Promega, cat#V5113) at 30°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were diluted 8x in 50 mM ammonium bicarbonate to reduce the urea concentration to 1 M and protein digestion was performed overnight at 37 C by addition of 2 µg of trypsin (Promega) per sample.
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bioRxiv - Molecular Biology 2023Quote: Proteins bound to beads were reduced with 2 mM DTT in 2 M urea buffer and sequencing grade trypsin (Promega) was added to a final concentration of 5 ng/μl ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were diluted with 50 mM NH4HCO3 to a final concentration of 2 M urea and digested with trypsin (Promega), at an enzyme-to-substrate ratio of 1:20 ...
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bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
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bioRxiv - Neuroscience 2020Quote: 1μg of total RNA from the human and mouse brain specimen was reverse-transcribed using the M-MLV reverse transcriptase (Promega) for efficient synthesis of the first-strand cDNA ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA (1.2 µg) was utilized to reverse transcript with M-MLV Reverse Transcriptase kit (M1701, Promega), Random Hexamer Primers (48190011 ...
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bioRxiv - Neuroscience 2020Quote: ... 10.67 % m/v PEG8000 (Promega), titrated amount of home-made Tn5 enzyme) ...
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bioRxiv - Plant Biology 2021Quote: ... M-MLV Reverse Transcriptase (Promega) was used for reverse transcription on two μg of DNA-free RNA.
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bioRxiv - Physiology 2020Quote: ... M-MLV Reverse Transcriptase (Promega) and a set of random hexamers were used with 1 μg total RNA following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... The urea concentration was then diluted to 1 M with 50 mM ABC and the proteins digested with trypsin (Promega, V511c) at 1:100 mass-ratio at 37 °C overnight.
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bioRxiv - Systems Biology 2019Quote: ... The samples were then diluted with 0.1 M NH4HCO3 to a final concentration of 1 M urea and the proteins were digested with sequencing-grade porcine trypsin (Promega, Switzerland) at a final enzyme:substrate ratio of 1:100 (w/w) ...
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bioRxiv - Biophysics 2023Quote: ... Cross-linked samples for XL-MS were digested by 1:50 (m/m) trypsin (Promega) overnight at 37 °C while shaking at 600 rpm ...