Labshake search
Citations for Promega :
1 - 50 of 4673 citations for Mouse Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2021Quote: ... We also included N-terminal and C-terminal HiBiT tags (Promega, Madison, WI) for quantification using luminescence ...
-
bioRxiv - Biochemistry 2023Quote: ... with the C-terminal HiBiT-Tag (Promega), using the In-Fusion cloning kit ...
-
bioRxiv - Biochemistry 2023Quote: N-terminal large (LgBit) and C-terminal small (SmBit) fragments derived from NanoLuc (Promega) were fused to the C-terminus of B56δ and inserted downstream of its N-arm at residue 103 ...
-
bioRxiv - Molecular Biology 2023Quote: ... C-terminal large bit (LgBiT) tagged construct (Promega) which is transcribed under control of the HSV-TK promoter.
-
bioRxiv - Molecular Biology 2023Quote: ... and MSN(T558A) genes with either N-terminal or C-terminal SmBit and LgBiT fusions were produced using Flexi®-based cloning (Promega). Best luminescence signals were obtained with CD44 containing a C-terminal smBiT fusion (C-terminal smBiT ...
-
bioRxiv - Cell Biology 2023Quote: ... we isolated individual clones and screened for expression using the C-terminal nanoluc fusion (Nanoluc Lytic Detection Kit, Promega), followed by additional characterization of SMO agonist-induced ciliary accumulation via microscopy ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... + 0.1 μg of NSD2 PWWP1-NanoLuc® Fusion Vector DNA (C-terminal) (Promega) using X-tremeGENE HP DNA Transfection Reagent (Sigma) ...
-
bioRxiv - Synthetic Biology 2019Quote: ... Poly-C tails were added to the fragmented DNA using terminal deoxynucleotidyl transferase (Promega). Then ...
-
bioRxiv - Biochemistry 2022Quote: ... N-or C-terminal NanoLuc/Kinase fusions were encoded in pFN31K expression vectors (Promega), including flexible Gly-Ser-Ser-Gly linkers between Nluc and each full-length kinase ...
-
bioRxiv - Molecular Biology 2021Quote: ... Rat Gβ1 was cloned with a C-terminal SmBiT34 (peptide 86 or HiBiT, Promega) connected with a 15AA polypeptide linker ...
-
bioRxiv - Physiology 2022Quote: ... Peptides contained C-terminal biotinylations and were spotted on streptavidin-conjugated membranes (Promega #V2861) and imaged with Typhoon FLA 7000 phosphorimager (GE ...
-
bioRxiv - Cell Biology 2021Quote: ... Eluted proteins from control HeLa cells with C-terminal mEGFP tag were digested with mass-spectrometry grade Lys-C and trypsin (Promega). Eluted proteins from a cell line lacking full-length Cdc20 (CDC20_M1-fs-M43 ...
-
bioRxiv - Molecular Biology 2023Quote: ... was removed by evaporation using speed-vac and the residue was used to produce L-[2,3,4,5-3H]-proline labeled procollagen chains and the collagen-like proteins in the TnT® Coupled Reticulocyte Lysate Systems (Promega) using either SP6 or T7 promoters ...
-
bioRxiv - Biophysics 2021Quote: ... Human Gβ with a C-terminal 15-amino-acid polypeptide linker followed by a HiBiT (peptide 86, Promega) and human Gγ were cloned into pFastBac vector ...
-
bioRxiv - Microbiology 2020Quote: ... Cytosine tails (C-tails) were added to 1 µg sheared DNA using terminal deoxynucleotidyl transferase (TdT, Promega, Madison, WI). Transposon containing fragments were amplified in a PCR containing DNA from the TdT reaction as template and primers specific to the ColE1 site on the 5’ end of the transposon ...
-
bioRxiv - Molecular Biology 2022Quote: ... The human Gβ1 with a C-terminal 15-amino acid polypeptide linker was followed by a HiBiT (peptide 86, Promega), and the scFv16 was modified with an N-terminal GP67 signaling peptide and a C-terminal 8× histidine tag ...
-
bioRxiv - Molecular Biology 2023Quote: ... Full-length GID4 N or C-terminal NanoLuc-fusion was transfected into HEK293T cells using FuGENE HD (Promega, E2312) according to manufacturer’s instructions and proteins were allowed to express for 20 hours ...
-
bioRxiv - Microbiology 2024Quote: ... Poly-C-tails were added to 1 µg of each end-repaired sample in duplicate using Terminal Transferase (Promega) with a ratio of 9.5 mM dCTP to 0.5 mM ddCTP to limit chain length and DNA Duplicate reactions were combined and purified with SPRIselect beads ...
-
bioRxiv - Biochemistry 2020Quote: ... cDNAs were obtained from Origene in pCMV6 vector that also incorporates C-terminal Myc and FLAG tags (Myc-FLAG) or subcloned into a pHTC (Promega)-derived vector that expresses C-terminal HaloTag (41) ...
-
bioRxiv - Genomics 2019Quote: To generate recombinant p53 we in vitro transcribed/translated human p53 with a c-terminal HA tag using a rabbit reticulocyte system (Promega). To generate fragmented genomic DNA we tagmented 50ng of human genomic DNA from MCF7 cells using the MuSeq kit (Thermo ...
-
Multimodal tubulin binding by the yeast kinesin-8, Kip3, underlies its motility and depolymerizationbioRxiv - Biophysics 2021Quote: ... An N-terminal HALO tag (Promega) for fluorescent labelling was fused to the coding sequence through a GGSGGSLQ linker ...
-
bioRxiv - Molecular Biology 2023Quote: ... The ImProm-II reverse transcription kit (Promega) was used to synthesize cDNA from 1 µg of total RNA using the included oligo dT primers in a volume of 20 µL per the manufacturer’s direction ...
-
bioRxiv - Neuroscience 2023Quote: ... by a terminal Deoxynucleotidyl transferase (Promega, M1875) with a specificity of 2.5 x 106 cpm/ml (C-Fos ...
-
bioRxiv - Cell Biology 2020Quote: To compare Promega’s NanoBiT system β-arrestin2 was inserted at the C-terminal end of LgBiT using Promegas pBiT1.1-N vector (Promega Cat. #N2014) and the doubly palmitolyated fragment of GAP43 was inserted at the N-terminal of SmBiT using the pBiT2.1-C vector.
-
bioRxiv - Microbiology 2019Quote: ... we replaced the C-terminal V5 tag with either HaloTag or NanoLuc (NLuc) from the NanoBRET Nano-Glo Detection System (Promega, Madison, WI). As a negative control ...
-
bioRxiv - Cell Biology 2021Quote: ... and 30 units of terminal deoxynucleotidyl transferase (Promega) in 20 μl of transferase buffer at 37°C for one hour The reaction was halted by incubation at 70°C for 10 minutes ...
-
bioRxiv - Molecular Biology 2023Quote: ... N-terminal small bit (SmBiT) tagged construct (Promega), with the native HSV-TK promoter swapped out for a CMV promoter ...
-
bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized with the ImProm II Kit (Promega). qPCR was performed using the Brilliant III Ultra-Fast SYBR® Green QPCR Master mix reagents (Agilent Technologies ...
-
bioRxiv - Immunology 2020Quote: ... TUNEL (terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling) staining was performed using the DeadEnd kit (Promega, PRG7130) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was carried out with commercial kit (Promega, Madison, WI, USA) following the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Human PAR2 with N-terminal nano-luciferase (nLuc, Promega) and C-terminal enhanced Yellow Fluorescent Protein (eYFP ...
-
bioRxiv - Molecular Biology 2021Quote: ... An ImProm-II™ Reverse Transcriptase System kit (Promega, USA) was used to synthesize the first strand cDNA using 1.5 μg of the DNA-free RNA sample as a template and each reaction was carried out according to the supplier’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... cDNA was obtained using an ImPROM II kit (Promega, A3800) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... pancreatic sections were submitted to terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay using a DeadEnd Fluorometric TUNEL system kit (Promega) according to the manufacturer’s instructions ...
-
Glycogen Synthase Kinase 3 regulates the genesis of the rare displaced ganglion cell retinal subtypebioRxiv - Neuroscience 2021Quote: ... Apoptosis was detected by terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) assays using in situ cell death detection kit (Promega). All images were acquired using a Zeiss LSM710 confocal microscope and Zen software (Zeiss).
-
bioRxiv - Genetics 2021Quote: Terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) staining (Promega) was used to visualize fragmented gDNA within paraffin-embedded RV tissue sections ...
-
bioRxiv - Genetics 2019Quote: RNA was isolated from single pupal terminal samples dissected at 24 hours APF or 28 hours APF using the Maxwell® 16 Tissue DNA Purification Kit (Promega). Poly-A RNA-seq libraries were generated using a Clontech library preparation kit (040215) ...
-
bioRxiv - Microbiology 2021Quote: N-terminal HaloTag KIF4 expressing plasmid (pFN21ASDB3041) was purchased from Promega. The N-terminal Myc-tagged KIF4 (both wild type and ATPase-null motor inactive mutant ...
-
bioRxiv - Neuroscience 2022Quote: ... Luminescence was read after adding 50μL Luciferase Assay Reagent II (Promega kit), then read a second time after addition of 50μL/well of stop and glow reagent ...
-
bioRxiv - Genomics 2023Quote: ... Reverse transcription was done using cDNA ImProm-II reverse transcription kit (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: SMC5/6 hexamer purified from E.coli with a C-terminal HALO tag on Nse2 was incubated with a 2-fold molar excess of Janelia-Fluor646 HaloTag Ligand (Promega; 12 µM Smc5/6 hexamer + 24 µM label). After incubation for 1 h at 25°C ...
-
bioRxiv - Cell Biology 2022Quote: ... terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL, Promega, Madison, WI, USA), immunohistochemistry (IHC) ...
-
bioRxiv - Cancer Biology 2019Quote: ... cDNA was yielded from purified mRNA using ImProm II Reverse Transcription kit (Promega). Quantitative-RT PCR was then performed using the SensiFAST SYBR Green Hi-ROX master mix (Bioline) ...
-
bioRxiv - Cell Biology 2019Quote: ... RT reaction was carried out in 15 ul reaction mixture at 40 °C for 60 min after addition of ImProm II (Promega). DNA fragments for L chain and the variable region of H chain (VH ...
-
bioRxiv - Cancer Biology 2019Quote: ... Complementary DNA (cDNA) was prepared using ImProm-II™ reverse transcription kit (Promega, A3800) and 10 ng of cDNA were used as template for each real-time PCR reaction ...
-
bioRxiv - Pathology 2023Quote: ... We reverse-transcribed cDNA from total RNA using the ImProm II Kit (Promega, US) with Random primers ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected with NOCT N-terminal mutant constructs using Fugene HD (Promega) in a ratio of 3 µL transfection reagent per 1 µg DNA ...
-
bioRxiv - Genetics 2021Quote: ... and cDNA was synthesized by ImProm-II™ Reverse Transcriptase kit (Promega, Madison, WI, USA). qRT-PCR was performed by using StepOne Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Plant Biology 2021Quote: ... and converted to cDNA using the ImProm-II Reverse Transcription System kit (Promega, Madison, USA).
-
bioRxiv - Genomics 2021Quote: HDAC activity assays were performed using the HDAC GLO I/II assay kit (Promega, G6430) in 96 well plates following the manufacturer’s instructions ...