Labshake search
Citations for Promega :
1 - 50 of 5936 citations for Mouse Lymphoid Enhancer Binding Factor 1 LEF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2023Quote: The enhancer region containing the FXR binding element was amplified by PCR and cloned into the pGL4.23 vector (Promega). HepG2 cells were transiently transfected with FXR (100 ng/well ...
-
bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
-
bioRxiv - Molecular Biology 2020Quote: pCDH-WSB1 and pGL4.14-c-Myc promoter-luciferase plasmids as well as pCDH-WSB1 and pGL4.14-TCF/LEF1-luciferase plasmids were transfected on H460 and tested with the Luciferase reporter kit (Promega) in the same way ...
-
bioRxiv - Zoology 2020Quote: ... or pGL3-enhancer plasmid (negative control; Promega). The firefly luciferase readings were normalized by the Renilla luciferase reading to calculate the relative fold-change.
-
bioRxiv - Biochemistry 2022Quote: ... 0.01 % enhancer (ProteaseMAX™, V2071, Promega, USA) is predispensed into the wells ...
-
bioRxiv - Biochemistry 2024Quote: ... and 0.01% enhancer (ProteaseMAX, V2071, Promega, USA) and 1 % DMSO) ...
-
bioRxiv - Developmental Biology 2022Quote: ... All enhancer or mutant enhancer reporter constructs (200ng) were transfected with 20ng of pRL (Renilla luciferase control vector, Promega) and either empty vector (pcDNA3.1 ...
-
bioRxiv - Genetics 2020Quote: ... cloned into a pGL4.23 enhancer assay vector (Promega), and sequence-verified ...
-
bioRxiv - Genomics 2020Quote: ... DNA was subcloned into the enhancer barrier vector28 (gift from Dr. Laura Elnitski, NHGRI) or pGL4.23 for enhancer assays (Promega, Madison, WI). Constructs were prepared using the Plasmid MidiPrep Kit (Qiagen ...
-
bioRxiv - Cell Biology 2020Quote: OCA2 enhancer region was cloned from mouse genomic DNA into KpnI and XhoI sites of the pGL3 promoter vector (E1751, Promega). MITF proximal promoter was cloned into SacI and HindIII sites of the PGL4 promoter vector (E6651 ...
-
bioRxiv - Developmental Biology 2020Quote: ... and 200 ng purified recombinant mouse DLX2 protein in Gel Shift Binding Buffer (Promega). Unlabeled probe was added for ‘cold’ competition ...
-
bioRxiv - Molecular Biology 2021Quote: ... one containing the SV40 promoter and enhancer (pGL3 Control, Promega, described as pSV40-luc in this manuscript ...
-
bioRxiv - Molecular Biology 2022Quote: ... one containing the SV40 promoter and enhancer (pGL3 Control, Promega, described as pSV40-luc in this manuscript ...
-
bioRxiv - Bioengineering 2021Quote: ... a volume of binding buffer from PCR clean-up kit (Promega, A9282) was added followed by 2 µL (300 ng ...
-
bioRxiv - Microbiology 2020Quote: ... 0.2% v/v ProteaseMax ™ surfactant trypsin enhancer (Promega, Madison, WI), 5mM dithiothreitol ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The enhancer constructs were transfected 24 h after seeding using FuGENE6 (Promega, US) as the transfection reagent ...
-
bioRxiv - Neuroscience 2022Quote: ... Purified and digested DNA fragment was cloned into a pGL3-Enhancer vector (Promega) and the correct sequence was checked by Sanger sequencing ...
-
bioRxiv - Genomics 2022Quote: ... containing a minimal CMV-promoter for enhancer-assays or pGL4.15[luc2P/Hygro] (Promega, E6701) w/o any promoter for promoter-assays were first digested using EcoRV (New England Biolabs ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2.5ng ml-1 recombinant human fibroblast growth factor (rhFGF) (Promega, Cat#G5071, Madison, WI, USA), and 1% chicken embryonic extract (Seralab ...
-
bioRxiv - Genomics 2023Quote: Reporter assays were conducted in HCT116 cells using the secreted NanoLuc enhancer activity reporter pNL3.3 (Promega) and normalized against a constitutively active firefly luciferase reporter vector ...
-
bioRxiv - Molecular Biology 2021Quote: The pGL-AP-1 plasmid containing 6 consensus AP-1 binding sites was co-transfected with the pRL-CMV plasmid (Promega) into cells using TransIT 2020 transfection reagent (Mirus Bio ...
-
bioRxiv - Cancer Biology 2019Quote: ... and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE) (hPD-1 Effector Cells, hPD-1 ECs, Promega) were cultured in RPMI-1640 medium (Biowest ...
-
bioRxiv - Biochemistry 2021Quote: ... Halo-tagged ubiquitin-binding domains (UBDs) of TUBE (tandem-repeated ubiquitin-binding entities) was incubated with HaloLink resin (200 μL, Promega) in binding buffer (50 mm Tris⍰HCl ...
-
bioRxiv - Molecular Biology 2020Quote: ... The pellet was resuspended in 100-μL of 0.2% Protease Max Surfactant Trypsin Enhancer (Promega Cat# V2071), 50-mM ABC ...
-
bioRxiv - Cancer Biology 2021Quote: Each enhancer or promoter region was amplified from human genomic DNA and cloned into pGL4.10 [luc2] (Promega) containing a SNP (rs718960 ...
-
bioRxiv - Cell Biology 2023Quote: ... mouse anti-1-gal (1:1000, Promega Z378B), mouse anti-1-gal (1:1000 ...
-
bioRxiv - Physiology 2023Quote: ... 25 ng/mL fibroblast growth factor (bFGF; Promega), 1% penicillin streptomycin ...
-
bioRxiv - Developmental Biology 2022Quote: CEBPa promoter (−2kb) or enhancer region (Cooper et al., 2015) was cloned into the pGL3 basic backbone (Promega). The construct containing part of the Id1 promoter region (336 bp ...
-
bioRxiv - Molecular Biology 2019Quote: Candidate enhancer sequences were synthesized as dsDNA (IDT) and cloned into the pGL4.24 destabilized Luciferase reporter vector (Promega) using the HiFi DNA Assembly method (NEB) ...
-
bioRxiv - Genomics 2022Quote: ... HepG2 cells were seeded in 24-well plates at a density of 100,000 cells/well and transfected with 400 ng pGL4.23-enhancer vector + 40 ng pRL-TK renilla vector (Promega) with Lipofectamine 3000 reagent ...
-
bioRxiv - Physiology 2023Quote: ... the oligo with NF-κB consensus binding element (Promega) was end-labeled by T4 polynucleotide kinase (Promega ...
-
bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
-
bioRxiv - Developmental Biology 2019Quote: ... mouse anti-LacZ (1/1000; Promega), mouse anti-βPS (1/10 ...
-
bioRxiv - Neuroscience 2022Quote: ... mouse anti-Lacz (1:200)(Promega), rabbit anti-CD4 (1:250)(Invitrogen) ...
-
bioRxiv - Cell Biology 2022Quote: ... anti-Halo (1:1000, mouse, Promega), anti-Myc (1:100 ...
-
bioRxiv - Biochemistry 2024Quote: ... 7C (mouse, Promega cat# N700A; 1:500-1:1000); Nluc in Fig ...
-
bioRxiv - Genomics 2021Quote: ... Variant or variant haplotype fragments were amplified in African American heterozygous individuals carrying the variants of interest located in the middle of the fragments (Supplementary Table 2 and 3) and cloned into the enhancer reporter vector pGL4.24 (E8421, Promega). Subsequently ...
-
bioRxiv - Molecular Biology 2020Quote: Prg4 enhancer/promoter-firefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector (Promega). Bovine genomic DNA was extracted from bovine chondrocytes and was used as the template to amplify Prg4 regulatory regions ...
-
bioRxiv - Molecular Biology 2020Quote: Prg4 enhancer/promoter-firefly luciferase reporters were co-transfected together with the pGL4.75 [hRlu/CMV] renilla luciferase reporter (Promega) into either superficial zone chondrocytes ...
-
bioRxiv - Neuroscience 2021Quote: Luciferase reporter constructs were constructed by cloning a candidate enhancer region into the pGL3 promoter vector (Promega; Madison, WI). Each region was inserted using standard restriction enzyme-based cloning techniques ...
-
bioRxiv - Genomics 2020Quote: ... Each well was transfected with 25ng of the corresponding putative enhancer-cloned pGL3-promoter luciferase reporter along with 1.5ng Renilla (pRL-TK Vector, Promega) control reporter vector using FuGENE HD transfection reagent as previously described [20] ...
-
bioRxiv - Genomics 2022Quote: MM001 and MM047 were seeded in 24-well plates and transfected with 400 ng pGL4.23-enhancer vector + 40 ng pRL-TK renilla vector (Promega) with Lipofectamine 2000 (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2022Quote: Luciferase reporter constructs were generated by cloning a candidate enhancer region into the pGL3 promoter vector (Promega; Madison, WI). Each region was inserted using standard restriction enzyme-based cloning techniques ...
-
bioRxiv - Genomics 2023Quote: ... Reference allele enhancer sequences for hZRS and hs737 were obtained via PCR cloning from human genomic DNA (Promega, G304A). Primers used for each enhancer sequence are outlined in Table S2 ...
-
bioRxiv - Biophysics 2022Quote: ... Factor Xa protease (SKU PR-V5581, Promega, Madison, WI) was used to cleave MBP-Q44-HttEx1 at 22 °C (Boatz et al. ...
-
bioRxiv - Physiology 2022Quote: ... 1:1000 mouse anti-ATP1A1 followed by 1:2500 goat anti-mouse HRP-conjugated secondary (catalog #: W4021; Promega). p-ATP1A1Y260 and ATP1A1 bands were visualized with SuperSignal Pico utilizing the ChemiDoc.
-
bioRxiv - Cell Biology 2019Quote: ... mouse anti-β-galactosidase (Promega, 1:2000); mouse anti-γ-tubulin (Sigma ...
-
bioRxiv - Microbiology 2020Quote: ... 1:1000 TuJ1 (monoclonal mouse, G712A, Promega); 1:250 NeuN (polyclonal chicken ...
-
bioRxiv - Neuroscience 2019Quote: ... and anti-mouse (1:3000; Promega #W4021) were used for detection with chemiluminescence (HyGLO Chemiluminescent HRP Antibody Detection Reagent ...
-
bioRxiv - Cell Biology 2019Quote: ... mouse anti-β-gal (Promega 1:500), chicken-anti-GFP (Invitrogen 1:1000) ...