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Citations for Promega :
1 - 50 of 3646 citations for Mouse Alpha ketoglutarate dependent dioxygenase FTO FTO ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
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bioRxiv - Neuroscience 2023Quote: ... 22F cAMP dependent reporter plasmid (Promega), and Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Synthetic Biology 2021Quote: ... SRE-dependent firefly luciferase (95ng; Promega; E1340) 60 ...
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bioRxiv - Neuroscience 2023Quote: ... cAMP-dependent GloSensor reporter plasmid (−22F cAMP plasmid, Promega, E2301), and Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Ca2+-dependent nuclear factor of activated T-cells (NFAT) (pGL4.30; Promega), or the MAPK signaling pathway (pGL4.33 ...
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bioRxiv - Microbiology 2019Quote: ... antibody-dependent cell-mediated cytotoxicity (ADCC) bioassays (29) were performed as prescribed by the manufacturer (ADCC reporter bioassay, complete kit, Promega, cat # G7010). In brief ...
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bioRxiv - Bioengineering 2022Quote: ... and the ATP dependent CellTiter-Glo® 2.0 Cell Viability Assay (#G9241, Promega, Dübendorf, Switzerland) according to the manufacturer procedure respectively ...
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bioRxiv - Biochemistry 2022Quote: ... we examined the downstream cAMP-dependent CREB-luciferase reporter system (pGL4.29 [luc2P/CRE/Hygro]; Promega). HEK293T or A cells were maintained in DMEM (InVitrogen ...
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bioRxiv - Microbiology 2021Quote: ... knowlesi elongation factor-1 alpha (pkef1-alpha) 5’ UTR were isolated from agarose gels and cloned into the pGEM-T vector (Promega, Madison, WI, USA). To replace the pfcam 5’ UTR sequence that drives luciferase transcription in the pD-pfcam-Luc ...
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bioRxiv - Synthetic Biology 2021Quote: ... we used a different cAMP-dependent firefly luciferase suitable for Gαi signaling (GloSensor-22F; Promega; E2301) 77 ...
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bioRxiv - Molecular Biology 2021Quote: ... Lectins were independently tested for cellular toxicity in an ATP-dependent assay (Cell-Titer Glo, Promega) and cells found to exhibit >80% viability up to a concentration of 200 μg/ml (data not shown).
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bioRxiv - Cancer Biology 2021Quote: Antibody-dependent cell-mediated cytotoxicity (ADCC) was assessed by an ADCC Reporter Bioassay (Promega, Madison, WI) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by addition of 25 uL/well of Antibody Dependent Cellular Cytotoxicity (ADCC) Bioreporter Cells (Promega cat# G7010). The ADCC Bioreporter cells are engineered Jurkat cells stably expressing FcγRIIIa V158 (high affinity ...
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bioRxiv - Biochemistry 2023Quote: ... Bovine alpha-2-hs-glycoprotein was isolated from plasma (Fetuin, Promega, V4961), and monocyte differentiation antigen CD14 was recombinantly expressed in CHO cells (R&D systems ...
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bioRxiv - Bioengineering 2022Quote: ... The cell viability was investigated through the LDH dependent CytoTox 96 Non-radioactive Cytotoxicity assay (#G1780, Promega, Dübendorf, Switzerland.) and the ATP dependent CellTiter-Glo® 2.0 Cell Viability Assay (#G9241 ...
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bioRxiv - Microbiology 2021Quote: ... and Tat-dependent increase of luciferase enzyme activity in cell lysates was determined with the Luciferase Assay system (Promega). Luminometric activity was analyzed with a Mithras luminometer.
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bioRxiv - Molecular Biology 2020Quote: ... RNA-dependent interactions were assessed by treating nuclear extracts with 1 U/μl RNasin® Ribonuclease Inhibitors (Promega, # N2615) as control or 1 mg/ml of RNase A (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... Time-dependent bioluminescence was measured after glutamine addition using Nano-Glo® Dual-Luciferase® reporter assay system (Promega). Cell culture plates were equilibrated at 22 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Triplicate biological replicates were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described68 ...
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bioRxiv - Cancer Biology 2022Quote: ... Quadruplicate biological replicates were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described 60 ...
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bioRxiv - Plant Biology 2019Quote: ... ATP content was deter-mined for each sample through ATP-dependent luciferase reaction (BacTiter-Glo Microbial Cell Viability Assay, Promega) as indicated by the manufacturer.
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bioRxiv - Microbiology 2022Quote: ... Replicon activity was calculated as the ratio of Renilla (subgenomic reporter) to Firefly (co-transfected loading control RNA, cap-dependent translation) using Dual-Luciferase Stop & Glo Reporter Assay System (Promega) and normalized by the same ratio for the control WT replicon ...
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bioRxiv - Microbiology 2022Quote: ... Replicon activity was calculated as the ratio of Renilla (subgenomic reporter) to Firefly (co-transfected loading control RNA, cap-dependent translation) using Dual Luciferase Stop & Glo Reporter Assay System (Promega) and normalized by the same ratio for the control wt replicon ...
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bioRxiv - Cell Biology 2024Quote: ... biological replicates (n=7) were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described (Hubner et al. ...
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bioRxiv - Synthetic Biology 2021Quote: HEK cells were transfected as previously described for the SRE reporter assay with the exception of substituting the SRE reporter with CRE-dependent firefly luciferase 60 (Promega; E8471).
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bioRxiv - Cell Biology 2022Quote: ... Baseline fluorescence was measured in an ELISA reader (Promega) following an incubation of 30 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... we confirmed that each primer set amplified single bands in a ligation- dependent manner by GoTaq Hot Start Green Master Mix (Promega, Madison, WI) (Figure S6) ...
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bioRxiv - Immunology 2023Quote: ... Three biological replicates for each condition were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) “on beads” as previously described [44] ...
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bioRxiv - Immunology 2021Quote: ... DNA was extracted from mouse tail using genomic DNA extraction kit (Promega Wizard SV Genomic DNA Purification System ...
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bioRxiv - Cancer Biology 2021Quote: ... Biological triplicates were digested with Lys-C (Alpha Laboratories, Eastleigh, UK) and trypsin (Promega, Madison, WI, USA) on beads as previously described (56) ...
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bioRxiv - Biophysics 2023Quote: ... ELISA plates were read on a GloMax plate reader (Promega, Wisconsin, US). Data were converted from concentration to total mass based on the volume of media/pellet ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from FACS purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively.
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bioRxiv - Genetics 2023Quote: RNA was isolated from mouse liver using a Maxwell 16 LEV simplyRNA kit (Promega). For the American vs standard diet comparison strand-specific mRNA libraries were generated using the TruSeq Stranded mRNA protocol (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... pH 8.0 using Vivaspin columns (3 kDa) and digested separately overnight using trypsin chymotrypsin or alpha lytic protease (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...
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bioRxiv - Immunology 2020Quote: ... Total IgG and ANA ELISA plates were read using a GlowMax Discover Microplate Reader (Promega) on the absorbance setting at 450nm.
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bioRxiv - Immunology 2022Quote: ... ELISA plates were immediately read using a GloMax®-Multi Detection System microplate reader (Promega) at 450nm absorbance.
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bioRxiv - Developmental Biology 2021Quote: ... total RNA was extracted from mouse uterus and reverse transcribed into cDNA by reverse transcriptase kit (Promega). cDNA was amplified by gene specific primers ...
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bioRxiv - Cancer Biology 2020Quote: ... after isolating DNA from mouse tissue samples using a tissue DNA extraction kit (Maxwell® 16, Promega), the purified DNA was incubated and hydrolyzed enzymatically (EpiQuik ...
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bioRxiv - Genomics 2022Quote: ... DNA extraction from mouse liver tissue was performed using the Maxwell 16 Tissue DNA Purification kit (Promega) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Pellets were reconstituted and digested with endoproteinase Lys-C (Alpha Laboratories, UK) for 1 hour at room temperature and trypsin (Promega, Madison, WI, USA) overnight at 35°C.
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bioRxiv - Cancer Biology 2020Quote: ... The lysate obtained from each tumour sample was mixed at 1:1 ratio with the super-SILAC standard prior to FASP digestion41 with Endoproteinase Lys-C (Alpha Laboratories, UK) and trypsin (Promega, Madison, WI, USA).
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bioRxiv - Cancer Biology 2024Quote: RNA from mouse tissue and from cell lines was extracted using the Maxwell RSC simplyRNA Cells Kit (Promega) on the Maxwell RSC instrument according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... ELISA was performed on the homogenate using the BDNF Emax Immuno Assay Systems (Promega KK, Tokyo, Japan). The hippocampus (HPC ...
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bioRxiv - Immunology 2023Quote: ... ELISA plates were immediately read using a GloMax®-Multi Detection System microplate reader (Promega, Madison WI) at 450 nm absorbance.
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bioRxiv - Developmental Biology 2021Quote: ... and mouse cyclin B1 were generated using the Riboprobe in vitro Transcription Systems kit according to the manufacturer’s protocol (Promega). Anti-BrdU antibodies were conjugated with protein A-sepharose beads overnight at 4C (GE Healthcare) ...
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bioRxiv - Neuroscience 2019Quote: ... Genomic DNA was isolated from tail snip samples with Maxwell® Mouse Tail DNA Purification Kit (Promega, Madison, WI). Polymerase chain reaction (PCR ...
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bioRxiv - Microbiology 2023Quote: The potential for M2e-specific antibodies to induce ADCC was evaluated using a mouse FcγRIV ADCC Reporter kit (Promega). In brief ...
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bioRxiv - Cell Biology 2019Quote: ... ELISA data was normalized to viable cell number determined by MTT assay (Boehringer Ingelheim) or CellTiter-Blue (Promega). IFN levels were determined using a HEK293T IFN reporter cell line (clone 3C11 ...
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bioRxiv - Physiology 2023Quote: ... GLP-1 was detected by indirect sandwich amide chemiluminescence ELISA using a luminescence plate reader (GloMax Promega, USA). This total GLP-1 assay detects GLP-1 (7-36 ...