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Citations for Promega :
1 - 50 of 321 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Competent Escherichia coli cells (E. coli JM109; Promega, Fitchburg, USA) were incubated with the ligation mixture on ice for 30 min and then transformed by a 45 sec heat shock at 42 °C ...
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bioRxiv - Synthetic Biology 2023Quote: ... His-tag proteins have been purified by using MagneHis system (Promega) and DynaMag spin magnet (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant was collected and purification was done via His-tag using HisLink Protein Purification Resin (V8823, Promega) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... S1 nuclease (Promega) or an equivalent amount of 1x S1 nuclease reaction buffer was added ...
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bioRxiv - Biochemistry 2023Quote: ... Rat Gβ1 was fused with a His-tag at the N terminus and with a SmBiT subunit (peptide 86, Promega)34 after a 15-amino acid linker at its C terminus ...
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bioRxiv - Molecular Biology 2020Quote: ... S1 nuclease (M5761, Promega), Mung Bean nuclease (M4311 ...
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bioRxiv - Microbiology 2023Quote: ... and random 9-mers in the presence of RNasin (Promega). qPCR was then performed on a Quantstudio 3 using the indicated targets and with iTaq Universal SYBR Green (BioRad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids were eluted with RNase free water and used as templates for in vitro gene expression (E. coli S30 Extract System for Circular DNA, Promega).
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmids were resuspended in RNase-free water and used as templates for in vitro gene expression (E. coli S30 Extract System for Circular DNA, Promega). Reactions were set up by adding 1 μL of 0.25 mM amino acid mix ...
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bioRxiv - Bioengineering 2019Quote: For S1 Nuclease (Promega, Cat. # M5761) digestion ...
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bioRxiv - Microbiology 2023Quote: The effect of pyocin SX2 on protein synthesis was assessed by an in vitro transcription-translation assay (E. coli T7 S30 Extract System for Circular DNA. Promega, Germany). pRL-SV40 vector (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... Bovine alpha-2-hs-glycoprotein was isolated from plasma (Fetuin, Promega, V4961), and monocyte differentiation antigen CD14 was recombinantly expressed in CHO cells (R&D systems ...
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bioRxiv - Molecular Biology 2023Quote: ... 1ng Luciferase Spike-In RNA (Promega), and 1X protease inhibitor cocktail ...
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bioRxiv - Developmental Biology 2021Quote: ... coli (Promega).
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bioRxiv - Developmental Biology 2021Quote: ... coli (Promega), and plasmid minipreps were prepared using the alkaline miniprep protocol by Wang et al ...
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bioRxiv - Molecular Biology 2019Quote: ... coli (Promega) were transformed with the expression vector ...
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bioRxiv - Biophysics 2019Quote: ... coli (Promega) or clinical isolate E ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli (Promega) were prepared using a Z-Competent E ...
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bioRxiv - Molecular Biology 2024Quote: ... coli (Promega) and grown on LB + 100 μg/ml carbenicillin (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... Buffer E (Promega) was added in cDNA and heated at 95 °C for 2 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... spike-in λ-DNA (Promega, Cat D150A) was added at a mass ratio of 1/200 ...
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bioRxiv - Microbiology 2021Quote: ... anti-Halo-tag (Promega), anti-FLAG (M2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Halo-Tag from Promega, USA (G9211) ...
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bioRxiv - Microbiology 2022Quote: 293T cells were co-transfected with pEGFP-N1 and plasmids expressing SARS-CoV-2 Spike (FL) or SARS-CoV-2 Spike-Δ19 for 24 h using FugeneHD (Promega). The cells were treated with DMSO or 10 µM 6-TG at 4 h post- transfection ...
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bioRxiv - Microbiology 2022Quote: ... and a spike-expressing plasmid (pCAGGS-SARS-CoV-2-spike) were co-transfected into HEK293T cells using Fugene HD transfection reagentia (Promega) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2023Quote: Vero E6 cells were transfected with an GFP expression vector together with either a control expression vector (no spike) or a SARS-CoV-2 spike expression vector using Fugene (Promega). Two hours after transfection ...
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bioRxiv - Biochemistry 2020Quote: ... coli KRX (Promega) were used for plasmid propagation and for protein production ...
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bioRxiv - Biochemistry 2020Quote: ... coli KRX (Promega) at 16°C using 0.1% l-rhamnose as inducer ...
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bioRxiv - Molecular Biology 2021Quote: ... coli KRX (Promega) which is a K12 derivative was used to express His6-Tus-GFP ...
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bioRxiv - Cell Biology 2021Quote: ... coli (Promega, L1195) and selected on LB plates containing 0.1 mg/ml ampicillin at 37°C overnight ...
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bioRxiv - Bioengineering 2022Quote: ... coli (Promega, JM109), and plated on LB-Ampicillin plates ...
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bioRxiv - Biophysics 2019Quote: ... coli cells (Promega) as described by Shen et al ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... coli DH5α (Promega) (cf ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cells (Promega) in 2xYT media with 25 µg/mL kanamycin and 2mM MgSO4 at 37°C to OD600 of 0.7–0.9 ...
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bioRxiv - Physiology 2019Quote: ... coli JM109 (Promega) cells ...
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bioRxiv - Microbiology 2020Quote: ... coli JM109 (Promega) was used for glutamate transporter complementation assays ...
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bioRxiv - Microbiology 2022Quote: ... coli JM109 (Promega), and E ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli KRX (Promega) cells were transformed by heat shock with the pET-25b+-RSL-MOA plasmid and pre-cultured in LB media substituted with 50 μg/mL ampicillin at 37°C under agitation at 180 rpm overnight ...
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bioRxiv - Bioengineering 2023Quote: ... coli (Promega, JM109), and plated on LB-Ampicillin plates ...
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bioRxiv - Bioengineering 2023Quote: ... coli (Promega, JM109), and plated on LB-Ampicillin plates ...
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bioRxiv - Cell Biology 2023Quote: ... Halo-Tag (Promega, G9211, mouse), and HSP90 (Santa Cruz Biotechnologies ...
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bioRxiv - Plant Biology 2024Quote: ... Magne Halo Tag Beads (Promega) were used to purify and capture the produced protein ...
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bioRxiv - Developmental Biology 2021Quote: ... Spike-in RNA (polyadenylated kanamycin mRNA #C1381, Promega, USA) was added to the Cell Lysis Buffer with a final concentration 107 molecules/ml ...
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bioRxiv - Microbiology 2020Quote: ... and 1 μg of a plasmid expressing the VSV G glycoprotein using the FuGene HD reagent (Promega). Forty-eight hours later ...
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bioRxiv - Microbiology 2023Quote: ... and 0.25 μg vesicular stomatitis virus G glycoprotein expression plasmids using the Fugene 6 transfection reagent (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... coli (Promega, Madison, WI) and isolated in large quantities using a ZymoPURE-EndoZero Maxiprep kit (Zymo Research ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli JM109 strain (Promega) was transformed with the violacein pathway plasmid (pTU2S(VioAE)-b-VioBCD ...
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bioRxiv - Plant Biology 2020Quote: ... coli KRX cells (Promega) was performed via heat shock method and subsequent selection on LB plates containing 50 mg/L kanamycin ...
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bioRxiv - Immunology 2022Quote: ... coli (Promega, Walldorf, Germany). Bacteria were grown over night on LB/amp/IPTG/X-gal plates and non-colored colonies were selected for plasmid preparation ...
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bioRxiv - Molecular Biology 2020Quote: ... coli JM109 (Promega L2005). Plasmids were isolated and purified from bacteria ...