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Citations for Promega :
1 - 50 of 227 citations for LACTB2 Human GST since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... GST or GST-OsDMI3 immobilized on the Magnet GST Particles (Promega) were incubated with His-OsMKK1/OsMKK6 in GST binding buffer (2 mM KH2PO4 ...
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bioRxiv - Developmental Biology 2021Quote: ... the GST tag was cleaved from GST-TES-1 using ProTEV Plus (Promega), according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... recombinant GST-tagged MARK2 (Promega) was incubated with tau at 30 °C at 1:100 ratio of MARK2:tau in phosphorylation buffer (25 mM PIPES ...
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bioRxiv - Molecular Biology 2020Quote: ... Human NOCT (1-431) or Schistosoma japonicum GST coding sequences were inserted into pFC3F using the Flexi Cloning System (Promega). To generate NOCT Δ(2-15)-3F ...
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bioRxiv - Cell Biology 2023Quote: HaloTag-GST (61 kDa) (Promega, G4491) was labelled with JF646 for 4 hours at 4 ° C on ice with 3x excess HaloTag ligand (buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... the GST region of psfRps29-GST was replaced with luciferase cDNA (pNL1.1[Nluc]Vector; Promega Madison, WI, USA). Luciferase cDNA was amplified via PCR using primers Nluc_FW2 and Nluc_RV2 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells grown on 10cm culture dishes were transiently transfected with the different HA-GST-RagC mutants and wildtype HA-GST-RagA using Fugene HD (#E2311, Promega). The following day ...
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bioRxiv - Microbiology 2023Quote: 1 ml of recombinant GST or GST-YgfB protein at a concentration of 10 μM was incubated with 100 μl 50 % MagneGST (Promega) bead-slurry equilibrated with pulldown-buffer for 45 min at 4°C and washed two times with 500 μl pulldown-buffer ...
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bioRxiv - Plant Biology 2023Quote: ... EIL1-GST and GST proteins were synthesized using the TnT SP6 High-Yield Wheat Germ Protein Expression System (Promega, L3260), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... we used Magne-GST Pull-Down Systems (Promega, USA). The bait ...
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bioRxiv - Biochemistry 2021Quote: ... GST-4xSUMO2 was purified using MagneGST Glutathione particles (Promega) as previously described150 ...
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bioRxiv - Plant Biology 2020Quote: ... GST-Halo was used as a protein standard (Promega). Incubate 1 μL of reticulocyte extract before binding with beads and 2 μL of extract after the binding was used for each western on a precast gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... HaloTag-GST was used as a negative control antigen (Promega).
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bioRxiv - Developmental Biology 2021Quote: ... The GST tag was removed from hTNPO1 using proTEV Plus Protease (Promega), and the protease enzyme was further removed from hTNPO1 protein by Ni-NTA Magnetic Beads (NEB ...
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bioRxiv - Cell Biology 2022Quote: GST-AIMP1 was in-gel digested with Trypsin GOLD (Promega, Madison, WI). Tryptic peptides were separated by reversed-phase chromatography using Eazynano LC II (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... GST-dimerized dynein constructs were labeled with 5 mM Halo-TMR (Promega) in the column for 10 min at room temperature and the unbound dye was washed away with TEV buffer at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... and GST-C-WRN were affinity-purified using glutathione (GSH)-magnetic beads (Promega).
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bioRxiv - Cell Biology 2023Quote: ... and GST-N-77 WRN were affinity-purified using glutathione (GSH)-magnetic beads (Promega). Fragment purification levels were assessed by SDS-PAGE followed by Coomassie staining.
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bioRxiv - Molecular Biology 2024Quote: ... GST-tagged AtCFI59 was purified using agarose beads (MagneGSTTM Glutathione Particles; Promega, Madison, USA) and retained on the beads to pull-down purified HIS-tagged AtCFI25a protein in TBS buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... GST fusion proteins were isolated by binding to glutathione magnetic beads (MagneGST™ Glutathione Particles, Promega) for 30 min at room temperature on a rotating wheel ...
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bioRxiv - Plant Biology 2022Quote: ... and GST- GBF3 were purified from 4 ml culture and immobilized on MagneGST Glutathione particles (Promega). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of GST-tagged effector proteins and 2 μl of FluoroTect™ GreenLys tRNA (Promega, USA) were gently mixed with High-Yield Wheat Germ Master Mix ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble GST and His fusion proteins were purified using the MagneGST Protein Purification System (Promega, USA) or the MagneHis Protein Purification System (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... The recombinant GST-OsNAC5 fusion protein was subsequently purified with the MagneGST™ Protein Purification System (Promega), following the provided protocol.
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bioRxiv - Plant Biology 2022Quote: ... His-tagged and GST-tagged recombinant proteins were purified using Promega MagneHis™ Ni-Particles (Promega Cat#V854A) and MagneGST™ Glutathione Particles (Promega Cat# V861A ...
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bioRxiv - Biophysics 2019Quote: ... Proteasomes were affinity purified with the bait protein GST-Ubl and magnetic beads (MagneGST™ Glutathione Particles, Promega) and eluted with purification buffer containing 25 mM reduced L-glutathione ...
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bioRxiv - Plant Biology 2019Quote: ... HA- and GST-tagged proteins were synthesized in vitro using the TNT® Coupled Wheat Germ Extract Systems (Promega). For the pull-down assays ...
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bioRxiv - Plant Biology 2020Quote: HALO-FDL1 and HALO-GST (negative control) DAP-seq samples were performed using protein expressed in the TNT rabbit reticulocyte expression system (Promega) as previously described (Bartlett et al. ...
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bioRxiv - Biochemistry 2019Quote: ... Dimeric constructs are based on VY208 and were created by artificially dimerization through an N-terminal GST-tag (Reck-Peterson et al, 2006) and tagged with a HaloTag (Promega) at the C-terminus as well as a GFP at the very N-terminus ...
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bioRxiv - Biochemistry 2021Quote: Pulldowns with cellular extracts were carried out as described for GFP-immunoprecipitations using 1-2 μg of GST-fused proteins bound to glutathione magnetic beads (Promega). For immunoprecipitations of recombinant proteins ...
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bioRxiv - Plant Biology 2021Quote: ... The GST-PIF3 and GST-PIF3mTAD constructs for GST pulldown assays were generated by subcloning PIF3 or PIF3mTAD into the EcoRI/XhoI sites of the pET42b vector (Promega). The PIF3p::HA-YFP-PIF3 and PIF3p::HA-YFP-PIF3mTAD constructs were generated by subcloning 2-kb PIF3 promoter ...
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bioRxiv - Cancer Biology 2022Quote: GSH/GSSG measurement was performed by a glutathione S-transferase (GST) enzyme coupled reaction using (GSH/GSSG Glo kit, Promega). Cells (2×104 ...
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bioRxiv - Microbiology 2022Quote: Flag-Trim69 was cloned downstream of a gluthathione-S-transferase (GST) in the pTKPL vector (an in-house derivative of the vector pGEXTK, Promega) and purified from bacterial lysates on glutathione agarose beads (Sigma ...
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
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bioRxiv - Neuroscience 2020Quote: ... All designed probes were tested by ddPCR using human genomic DNA (Human male, Promega) as a negative control ...
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bioRxiv - Molecular Biology 2022Quote: ... Human Genomic DNA (G1521, Promega) was used as positive control and synthetic DNA was used to validate the LAMP assay performance ...
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bioRxiv - Microbiology 2020Quote: ... 500 ng human genomic DNA (Promega) or 5 μl of untreated or RNase-treated nasal swab nucleic acid isolates ...
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bioRxiv - Cancer Biology 2020Quote: ... Pooled human male genomic DNA (Promega) was randomly sheared (500-800bp ...
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bioRxiv - Systems Biology 2021Quote: ... a human cDNA pool (Promega Corporation), or obtained as synthesized double-stranded DNA fragments (gBlocks ...
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bioRxiv - Genomics 2021Quote: ... 50ng human genomic DNA (Promega, G304A), 2µM assembled T7-Tn5 transposase or Tn5 transposase was incubated at 55 °C for 7minutes ...
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bioRxiv - Immunology 2023Quote: ... Lumit Human IL-1β Immunoassay (Promega) was performed according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... human Female genomic DNA (Promega #G1521), as a reference ...
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bioRxiv - Plant Biology 2021Quote: The cDNAs corresponding to the two alleles of SoIAA2 were cloned into a pFN2A (GST) flexi vector (Promega – primers in Supplementary Table 1), expressed in E ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacterial lysates expressing fused GST-CuLCrV / SiGMV / TYLCV CP proteins and GST control were incubated with MagneGST□ particles (cat#V8600, MagneGST protein purification system, Promega, Madison, WI, USA) to immobilize the GST tagged proteins as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Promega, Madison, WI, USA) was diluted to 10,000 genome copies per μl then serially diluted to 10 genome copies per μl and used to create a human DNA copy number standard curve ...
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bioRxiv - Molecular Biology 2019Quote: ... The natural templates include purified human (Promega), rhesus monkey (BioChain) ...
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bioRxiv - Molecular Biology 2019Quote: ... 10.0 ng/ml recombinant human FGF (Promega) and 1.0 μM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... Human Genomic DNA Male (Promega, cat # G1471) and IDH1 R132H Reference Standard (Horizon ...
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bioRxiv - Cancer Biology 2020Quote: ... 50ng of normal human genomic DNA (Promega) was used as a negative control ...
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bioRxiv - Cancer Biology 2021Quote: ... Human LAMA1 cDNA was purchased from Promega and sub-cloned into pLVX-Tet-On Advanced vector (Clontech) ...