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Citations for Promega :
1 - 50 of 1816 citations for L Phenylalanine N T Boc 2 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... t-ERK1/2 (Promega V114A, 1/1000 dilution), p-AKT (Cell Signaling Technology ...
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bioRxiv - Microbiology 2023Quote: ... Biotin-oligo d(T) (Promega, #Z5261, 0.2 μmol/L for mRNA FISH); Diethyl Pyrocarbonate (DEPC)-treated water (Invitrogen™ ...
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bioRxiv - Microbiology 2022Quote: ... The purified PCR products were subjected to A-tailing with Taq DNA polymerase and to ligation into a T-vector (pGEM®-T Easy Vector, Promega, cat. n° A1360). The ligation product was used to transform E ...
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bioRxiv - Cell Biology 2022Quote: ... β-arrestin 2 fused at the N-terminus to LgBiT (LgBiT-β-arrestin 2; Promega, plasmid no. CS1603B118) was chosen as it has previously been used successfully with other class B GPCRs ...
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bioRxiv - Microbiology 2022Quote: ... mutations were generated in a fragment of the L ORF subcloned into a pGEM T easy vector (Promega). The mutated ORF fragment was sequenced and then substituted in the L ORF of pTM1 ...
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bioRxiv - Molecular Biology 2023Quote: Plasmids used as DNA template in PCR reactions to generate donor DNAs for gene deletion/disruption strategies were obtained by amplifying blasticidin S deaminase (BSD) and puromycin-N-acetyltransferase (PAC) genes and cloning them individually into pGEM®-T Easy Vector (Promega). The reverse primers to amplify both genes included GTGA as four last nucleotides ...
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bioRxiv - Genomics 2021Quote: ... Membranes were washed three times in TBS-T before incubating with secondary antibodies (HRP-anti Mouse-IgG (H+L) (Promega)) for 45 min in TBS-T and 2% milk ...
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bioRxiv - Microbiology 2023Quote: ... 20 μL of MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) inner salt (Promega) was added to each well and the plate was further incubated for 1 h at 37 °C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... or into the pGEM-T or pGEM-T Easy vector (Promega) and subsequently sequenced (Macrogen).
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bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to a concentration of urea of 2 mol L-1 and digested overnight with the addition of 2 µl sequencing-grade trypsin (Promega). After digestion ...
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bioRxiv - Biochemistry 2019Quote: ... N-glycans were released from the membrane-bound protein using 2 U PNGase F (Promega, WI, USA) with overnight incubation at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plasmid for β-arrestin-2 fused at the N-terminus to LgBiT was obtained from Promega custom assay services (plasmid CS1603B118) ...
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bioRxiv - Cell Biology 2021Quote: HEK293 T-REx SNAPf-GLP-1R-SmBiT cells were seeded in 12-well plates and co-transfected with 0.1 μg β-arrestin-2 fused at the N-terminus to LgBiT (LgBiT-β-arrestin-2; Promega, plasmid no. CS1603B118) and either 0.1 μg mini-Gs-Venus or mVenus supplemented with 0.8 μg pcDNA 3.1.
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bioRxiv - Neuroscience 2023Quote: ... 10 µL of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) reagent (#G3582; Promega, Madison, WI) was added and incubated at 37°C for 45 mins ...
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bioRxiv - Microbiology 2020Quote: ... PCR#2 product was ligated to pGEM-T easy vector or TOPO vector following the manufacturer’s instructions (Promega). After transformation in bacteria and plasmid amplification ...
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bioRxiv - Developmental Biology 2023Quote: ... or pGEM-T (Promega) vectors and eight positive colonies/gene transferred to 750 ul of ddH2O and heated to 90 C to liberate the plasmids ...
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bioRxiv - Cancer Biology 2021Quote: T Cell Activation Bioassay (Promega) was performed according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... The pGEM T-easy (Promega) based positive control plasmid of NNV was constructed from a 605 bp RNA2 fragment amplified from NNV-infected orange-spotted grouper (laboratory sample) ...
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bioRxiv - Microbiology 2019Quote: ... and pGEM-T Easy (Promega) were used ...
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bioRxiv - Microbiology 2022Quote: ... pGEM-T Easy vector (Promega) (supplementary data table 6) ...
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bioRxiv - Developmental Biology 2023Quote: ... cloned into pGEM-T (Promega) and used to synthesize digoxigenin-labeled probes by in vitro transcription using SP6 or T7 polymerases (Thermo-Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... spxB gene region containing the ORF (amplified from R6 genome using primer set 1-2) was cloned into pGEM-T Easy vector (Promega). spxB ORF was disrupted by cloning a kanamycin resistance cassette (amplified from pIBD38 ...
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bioRxiv - Immunology 2023Quote: ... and the corresponding SARS-CoV-2 spike construct were co-transfected in HEK293-T cells using FuGENE 6 Transfection Reagent (Promega) in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Genomics 2019Quote: ... which were subsequently ligated to pGEM-T Vector (pGEM-T Vector System I, Promega, USA), and transformed to DH5α competent cells (Z-Competent E ...
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bioRxiv - Biochemistry 2020Quote: N-glycans were released from nMPO using Elizabethkingia miricola peptide-N-glycosidase F (Promega) (Jensen et al. ...
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were enzymatic released using 3.5 U Flavobacterium meningosepticum N-glycosidase F (Promega) in 20µl water/well ...
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bioRxiv - Microbiology 2020Quote: ... In vitro transcription was performed for EagI-cleaved YACs and PCR-amplified SARS-CoV-2 N gene using the T7 RiboMAX Large Scale RNA production system (Promega) as described previously26 ...
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bioRxiv - Immunology 2022Quote: ... In vitro transcription was performed for EagI-cleaved YACs and PCR-amplified SARS-CoV-2 N gene using the T7 RiboMAX Large Scale RNA production system (Promega) as described previously ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The plasmid for β-arrestin-2 fused at the N-terminus to LgBiT was obtained from Promega (plasmid no. CS1603B118). Construction of the GLP-1R-SmBiT plasmid was described previously (55 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plasmid for β-arrestin-2 fused at the N-terminus to LgBiT was obtained from Promega (plasmid no. CS1603B118); this configuration was used due to previous success with another class B GPCR (Shintani et al. ...
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bioRxiv - Microbiology 2022Quote: In vitro transcription was performed for EagI-cleaved YACs and PCR-amplified SARS-CoV-2 N gene using the T7 RiboMAX Large Scale RNA production system (Promega) as described previously [25] ...
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bioRxiv - Microbiology 2022Quote: In vitro transcription was performed for EagI-cleaved YACs and PCR-amplified SARS-CoV-2 N gene using the T7 RiboMAX Large Scale RNA production system (Promega) as described previously20 ...
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bioRxiv - Molecular Biology 2022Quote: ... pNLF1-N [CMV Hygro] (Promega), and pNLF1-C [CMV Hygro] (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then washed 3x with TBS-T and incubated for 2 hours at room temperature with either anti-rabbit (1:5000, Promega W4011) or anti-mouse HRP (1:5000 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... or a pGEM®-T Easy Vector (pGEM®-T Easy Vector Systems Kit, Promega, USA) depending on the Taq-polymerase used for PCR (blunt vs ...
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bioRxiv - Molecular Biology 2023Quote: ... and resulting PCR products were subcloned into a pGEM-T vector (Promega, pGEM-T Vector Systems) for subsequent Sanger sequencing of both alleles ...
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bioRxiv - Plant Biology 2019Quote: ... pGEM®-T kit from Promega were used ...
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bioRxiv - Immunology 2021Quote: ... or pGEM-T plasmid (Promega, UK), manufacturer’s instructions were followed ...
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bioRxiv - Developmental Biology 2021Quote: ... serial dilutions of pGEM-T (Promega) plasmids containing coding sequences of the target genes or internal control genes (rp49 for Drosophila melanogaster and AeRpL32 (AAEL003396 ...
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bioRxiv - Plant Biology 2020Quote: ... cloned in pGEM-t Easy (Promega), re-amplified by PCR with primers with attB tails and BP-cloned into pDONR207 (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: The pGEM-T donor vector (Promega) was engineered by introducing a donor construct ...
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bioRxiv - Molecular Biology 2020Quote: ... subcloned via pGEM-T easy (Promega) and inserted upstream of a chloramphenicol resistance cassette in the plasmid pSF36 (pUC19+cm-FRT-HindIII ...
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bioRxiv - Microbiology 2019Quote: ... cloned into pGEM-T Easy (Promega), and then inserted using the NotI site ...
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bioRxiv - Microbiology 2020Quote: ... ligated to pGEM-T Easy (Promega), and transformed into E ...
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bioRxiv - Molecular Biology 2021Quote: ... or pGEM-T-Easy vector (Promega). For cloning into pGEM-T-Easy vector ...
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bioRxiv - Plant Biology 2022Quote: ... ligated into pGEM-T (Promega, USA), and checked by sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... cloned in pGEM-T vector (Promega) and sequenced (Macrogen) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid pGEM®-T Easy (Promega) was used as a primary cloning vector ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was extracted individually from whole bees (test; n=300, control; n=165) by Promega Wizard® purification.