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Citations for Promega :
1 - 50 of 1679 citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... Proteins were co-precipitated with 1 µg trypsin (Promega) overnight at −20 °C in 1 ml methanol ...
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bioRxiv - Biochemistry 2021Quote: Mitochondrial 35S-labeled precursor proteins were synthesized using the TNT SP6-coupled transcription/translation kit (Promega) and plasmid-vectors pSP64 containing the genes of interest ...
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bioRxiv - Developmental Biology 2022Quote: ... 100 μg of mitochondrial protein in triplicate was incubated in GTPase Buffer (Promega #V7681; Madison, WI, USA) with 10 μM GTP and 1 mM DTT ...
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bioRxiv - Immunology 2022Quote: ... and the mitochondrial ToxGlo™ (Promega) assay were used accordingly to the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were co-expressed using TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Mitochondrial toxicity assay was performed using the “Mitochondrial ToxGloTM Assay” kit according to the manufacturer’s instructions (Promega). Briefly ...
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bioRxiv - Immunology 2023Quote: ... ATP was measured with Mitochondrial ToxGlo Assay kit (Promega) and followed the manufacturer’s protocol.
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bioRxiv - Biochemistry 2022Quote: ... The proteins were digested by 0.2 μg modified grade porcine trypsin (Promega, Co., Madison, WI) during overnight incubation at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... the pcDNA3.1+ expression plasmids for spike proteins were co-transfected using Fugene (Promega, Madison, WI, USA) into HEK293T/17 cells ...
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bioRxiv - Microbiology 2020Quote: The protein samples were digested with 500 ng of sequencing grade trypsin (V511, Promega Co., Madison, WI, USA) and incubated at 37 °C following the protocol described by Shevchenko et al.22 ...
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bioRxiv - Cell Biology 2022Quote: ... ATP levels were assayed using the Mitochondrial ToxGlo™ Assay kit (Promega) as per manufacturer’s instructions on a FLUOstar OPTIMA (BMG LABTECH ...
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bioRxiv - Physiology 2019Quote: ... Cytotoxicity and ATP generation were measured using mitochondrial ToxGlo assay (G8000) from Promega as per the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2019Quote: Iron-related cytotoxicity was monitored with a Luciferase-based viability assay (RealTime-Glo™ MT Cell Viability Assay, Promega) according to the protocol of the manufacturer in 96-well plate format as an endpoint measurement ...
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bioRxiv - Neuroscience 2019Quote: ... and co-transfected (TransFast protocol, Promega) with the test constructs (PureYield plasmid ...
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bioRxiv - Cell Biology 2024Quote: ... Protein detection was done using primary antibody anti-HaloTag monoclonal antibody (1:1,000 dilution, Promega), primary antibody anti-MamE polyclonal antibody (1:3,000 dilution ...
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bioRxiv - Genetics 2020Quote: ... Co-transfection was performed with ViaFect (Promega) reagent using previously optimized conditions 16 ...
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bioRxiv - Cell Biology 2022Quote: ATP levels were estimated using the ATP detection reagent of the Mitochondrial ToxGlo Assay kit (Promega). 50 μg mitochondria from WT and deletion strains grown at 30°C in YPG media were lysed by two times snap freezing the mitochondria in liquid nitrogen followed by thawing on ice ...
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bioRxiv - Biochemistry 2021Quote: ... 11S protein was detected with a rabbit polyclonal antibody from Promega, and Tom40 with a rabbit polyclonal antibody produced by Cambridge Research Biochemicals (Billingham ...
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bioRxiv - Immunology 2024Quote: Recombinant antibodies were purified using protein G magnetic beads (Promega #G7472), HiTrap protein G HP columns (Cytiva #17040401) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cytotoxicity and ATP Production were measured with the use of a Mitochondrial ToxGlo™ Assay Kit (Promega), as described in (Weatherly et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Reporters were co-transfected with Renilla luciferase (Promega) reporter as a transfection efficiency control ...
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bioRxiv - Cancer Biology 2023Quote: ... and then co-cultured with Bright-Glo (Promega). Fifteen minutes later ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell viability was determined at 72 h post-seeding in 96-well round-bottom ULA cluster plates using CellTiter-Glo® (Promega). Western blot analysis was performed on adherent cell lysates at 72 h post-seeding to confirm DUSP4 knockdown.
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bioRxiv - Molecular Biology 2024Quote: ... The level of ATP under different conditions was determined using the Mitochondrial ToxGlo™ Assay from Promega (UK), following the protocol provided by the manufacturer.
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were obtained in carrier/ protein-free buffer or were purified using the Magne Protein A or G Beads (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... and co-transfection with a Renilla luciferase plasmid (Promega) served as a transfection control ...
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bioRxiv - Cell Biology 2022Quote: ... COS-7 cells were transiently transfected with FuGENE6 (Promega) with different StrepII- GFP-CSPP1 constructs for 24 hours ...
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bioRxiv - Genetics 2020Quote: ... Vectors were co-transfected with pRL-SV40 Renilla (Promega) in a 20:1 ratio (2ug pGL3 to 100ng pRL-SV40) ...
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bioRxiv - Plant Biology 2021Quote: ... Protein production was confirmed by western blot using an anti-HaloTag antibody (Promega). HaloTag-ligand conjugated magnetic beads (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... Total cell and mitochondrial free cholesterol and cholesterol ester contents were measured using the Cholesterol/Cholesterol Ester-Glo Assay Kit (J3190, Promega) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2021Quote: ... were then used to amplify a 443 bp region of the mitochondrial cox1 gene using GoTaq Hot Start Master Mix (Promega). Each 50 µL reaction contained 500 nM each primer and cycling conditions were as described above ...
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bioRxiv - Molecular Biology 2020Quote: ... using GoTaq qPCR Master Mix (Promega Co., Madison, WI, USA). Primers used in this study were as follows:
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bioRxiv - Immunology 2023Quote: ... cells were co-transfected with 10µg TK-Renilla (#E2241, Promega) and 10µg HRE-luciferase (a gift from Navdeep Chandel ...
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bioRxiv - Developmental Biology 2024Quote: ... 1x T4 DNA ligase buffer (Promega Co., Cat. No. C126B), 1.5 U T4 DNA ligase (Promega Co. ...
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bioRxiv - Developmental Biology 2024Quote: ... 1.5 U T4 DNA ligase (Promega Co., Cat. No. M180A), 5 U BsmBI (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... The standard curve was generated by cloning a short 100-bp sequence of mitochondrial 16S rRNA gene into pGEM®-T Easy Vector (Promega). The 16S_rRNA gene sequence was amplified with Taq Polymerase (Supreme NZYtech II master mix ...
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bioRxiv - Neuroscience 2021Quote: The remodeling of neuron isolated mitochondria was evaluated according to a published protocol,[54] by measuring the changes in the absorbance of the mitochondrial suspensions at 540 nm using a GloMax Discover multimode plate reader (Promega, Italy). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were also co-transfected with 15 ng pTK-Renilla (Promega) plasmids using Lipofectamine 3000 reagent (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... Each well was co-transfected with the pRL-TK plasmid (Promega) expressing the renilla luciferase as control ...
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bioRxiv - Biochemistry 2020Quote: Packaging cells (HEK-293T) were co-transfected using Fugene HD (Promega) according to the manufacturer’s instructions with the packaging vector psPAX2 ...
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bioRxiv - Systems Biology 2021Quote: ... HEK293 cells were co-transfected using Fugene 6 transfection reagent (Promega) with MAC-tag (600ng ...
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bioRxiv - Immunology 2022Quote: ... Cells were co-transfected using FuGENE® HD Transfection Reagent (Promega) with Opti-MEM® diluted plasmids (450 ng of pCAGGS-SARS-CoV-2 spike ...
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bioRxiv - Immunology 2019Quote: ... These constructs were co-transfected to 293 cells by FugeneHD (Promega). Transfected cells were attached in a 96-well plate and then 0 ...
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bioRxiv - Genetics 2021Quote: ... Plasmids were co-transfected into LF2 mESCs using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... 0.01 Unit of DNase I (Promega Biotech Co., Ltd, catalog#: 137017) was added and the reaction mixture incubated for additional 5 minutes ...
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bioRxiv - Genomics 2022Quote: ... cells were co-transfected with 15 fmol pRL-TK (Promega, E2241) and 150 fmol of either pGL4.27[luc2P/minP/Hygro] empty vector or pGL4.27[luc2P/minP/Hygro] containing either eSOX17 ...
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bioRxiv - Neuroscience 2023Quote: ... we used co-transfection experiments with the reporter plasmid pGL4.30 (Promega) that contains upstream to the Firefly luciferase sequence 3x a binding sequence for NFAT ...
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bioRxiv - Cancer Biology 2019Quote: Mitochondrial activity of C-33A cells was measured with the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (MTS) (Promega, Madison, WI). Cells were seeded in 96-well plates (5000 cells/well ...
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged or HA-tagged protein was detected using a commercially available mouse monoclonal antibody (Promega).
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bioRxiv - Developmental Biology 2020Quote: ... which was co-transfected with 3 ng phRG-TK Renilla vector (Promega) as normalization control ...