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Citations for Promega :
1 - 50 of 323 citations for Interleukin 35 IL 35 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 35 µL of OneGlo reagent (Promega) were added and luminescence was read ...
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bioRxiv - Biochemistry 2022Quote: Radiolabeled proteins were synthesized in the presence of 35S-methionine and 35S-cysteine using either coupled reticulocyte lysate system (TNT, Promega) or uncoupled reticulocyte lysate system (Promega) ...
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bioRxiv - Genetics 2020Quote: ... mixed with 35 µL of nuclease-treated RRL (Promega) and 0.5 µL of 1 mM amino-acid mixture lacking Leu and Met ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed with 35 μl passive lysis buffer (Promega) and subjected to three freeze-thaw cycles ...
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bioRxiv - Microbiology 2021Quote: ... 35 and 42 were extracted with Wizard® Genomic DNA purification kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Lumit Human IL-1β Immunoassay (Promega) was performed according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Human 26S proteasome purified from HEK293 cells was purchased from Promega. The proteasome chaperoning reaction tests were carried out as follows ...
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bioRxiv - Microbiology 2021Quote: ... after which the media were removed and 35 µl of Passive Lysis buffer (Promega) was added to each well ...
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bioRxiv - Microbiology 2020Quote: ... then labeled with 35S-Methionine (Amersham Pharmacia Biotech) in vitro transcription-translation system (Promega), respectively ...
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bioRxiv - Developmental Biology 2020Quote: ... [35S]-labeled Zc3h10 protein was produced by using TNT coupled transcription/translation kit (Promega). 20 ug of GST fusion proteins were incubated for 2 hrs at 4°C with in vitro translated Zc3h10 and glutathione sepharose beads ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μL of buffer containing 35 mM iodoacetamide and Trypsin/Lys-C (Promega, V5072) was added to each sample ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μL of buffer containing 35 mM iodoacetamide and Trypsin/Lys-C (Promega, V5072) was added to each sample ...
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bioRxiv - Cell Biology 2022Quote: ... Protein synthesis was performed in the presence of [35S]methionine in rabbit reticulocyte lysate (Promega). Coupled translation/translocation reactions of 20 μl contained 16.5 μl of reticulocyte lysate ...
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bioRxiv - Microbiology 2022Quote: ... Radiolabelled [35S]ORF3c-5M was synthesised in vitro in Flexi Rabbit Reticulocyte Lysate system (Promega) in the presence of 0.5 mM of KCl ...
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bioRxiv - Biochemistry 2023Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (T7, Promega). Translation products were partially purified by ammonium sulfate precipitation [87] ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were resuspended in 35 μl of reaction buffer (0.2 U/μl RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... The radio-labelled probe was synthesised replacing UTP with 35S UTP using Riboprobe® System (Promega) for 40 min at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... [35S]-labeled proteins were synthesized in vitro via a coupled transcription/translation rabbit reticulocyte kit (Promega) incubated at 30°C for 90 min with [35S]Met/Cys metabolic labeling reagent (MP Biomedical ...
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bioRxiv - Biochemistry 2021Quote: Mitochondrial 35S-labeled precursor proteins were synthesized using the TNT SP6-coupled transcription/translation kit (Promega) and plasmid-vectors pSP64 containing the genes of interest ...
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bioRxiv - Molecular Biology 2022Quote: ... were incubated with 35S labeled proteins made by using TNT Quick Coupled transcription/Translation System (Promega) with 35S labeled methionine ...
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bioRxiv - Genomics 2023Quote: ... Nuclei were resuspended in 35 µL of reaction buffer (0.2 U/µL RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega). Translation reaction products from 0.25 μg of plasmid DNA were incubated with GST-labeled proteins on glutathione-Sepharose beads in NETN-E buffer (50 mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei were resuspended in 35 µL of reaction buffer (0.2 U/µL RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: KNL-2-MBP fragments were [35]S labeled using TnT® Quick Coupled Transcription/Translation System (Promega). 10 μl of the in vitro translation lysate was incubated for 1hr at 4°C with 50 μg CENP-AHCP-3(1-109)-MBP-His (in 20 mM Tris [pH7.5] ...
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bioRxiv - Cell Biology 2021Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (SP6 or T7, Promega). Translation products were partially purified by ammonium sulfate precipitation (Bardwell & Shah ...
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bioRxiv - Immunology 2021Quote: ... and used to generate 35S-methionine labelled AIM2 protein by in vitro transcription and translation (IVTT) (Promega). Immunoprecipitations (IP ...
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bioRxiv - Cell Biology 2022Quote: ... [35S]-methionine-labelled proteins were transcribed and translated using a TnT™ coupled transcription–translation kit (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... 35S labeled proteins were synthesized in vitro using the TNT T7 Quick for PCR DNA kit (Promega, L5540). Labeled proteins were incubated with MBP-tagged proteins in TNEN50 (50mM Tris pH7.5 ...
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bioRxiv - Microbiology 2021Quote: ... 35S methionine-labeled full-length cGAS and E5 was generated with the TnT Coupled Reticulocyte Lysate System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... virus-containing media was removed and replaced with 35 μl of 1x Passive lysis buffer (Promega; Ref# E194A). Plates underwent three freeze-thaw cycles consisting of freezing on dry-ice for 15 minutes followed by thawing at 37°C for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: 35S-radiolabeled Tom40 precursor proteins were synthesized in vitro using the TNT Quick Coupled Transcription/Translation kit (Promega). Transcripts for MCO6 encoding 3 additional N-terminal methionine residues for radiolabelling of the Mco6 protein which lacks internal methionine residues ...
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bioRxiv - Biochemistry 2021Quote: ... An aliquot (35 μg) was subjected to enzymatic hydrolysis “in solution” either with trypsin (Sequencing Grade Modified Trypsin, Promega) or with chymotrypsin (Analytical Grade ...
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bioRxiv - Neuroscience 2022Quote: ... to a final urea concentration of 2 M for overnight Trypsin/Lys-C digestion at 35°C (1:100 protease:substrate ratio, Mass Spectrometry grade, Promega Corporation ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed once in PBS and lysed in 35 μl 1x Luciferase Cell culture lysis buffer (E1531, Promega) for 15min at room temperature and centrifuged for 10min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The antisense radio-labeled 35S-Vglut2 riboprobe was generated by in vitro transcription using a T7 RNA polymerase (Promega). 35S-labeled riboprobe was diluted in hybridization solution (50% formamide ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed once in PBS and lysed in 35 μL 1× Luciferase Cell culture lysis buffer (E1531, Promega) for 15 min at room temperature and centrifuged for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... ATFS-11-100(R/R)::GFP and Su91-69::GFP) were synthesized in reticulocyte lysate in the presence of [35S]methionine (T7 Quick for PCR DNA, Promega). Import into isolated mitochondria was performed in import buffer (3 % (w/v ...
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bioRxiv - Cancer Biology 2022Quote: ... a gene-specific amplicon was generated in a 20 μL reaction for 35 cycles with GoTaq G2 Green Master Mix (Promega). The PCR product was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 h followed by a second digest with trypsin at 35°C overnight at 1200 rpm (1:50 ratio w/w, Promega). Proteolysis was stopped by the addition of 1% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were incubated for 3 days at 35°C with 5% CO2 and cell viability was evaluated using the CellTiter Glo (Promega) post the 3-day incubation ...
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bioRxiv - Biophysics 2020Quote: ... Plasmids were transfected in cells (passage number < 35) seeded in 96-well plates at ~50 % confluence 2-4 days before the experiment with FuGene6 (Promega) or Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was extracted from about 35 mg of tissue using the Maxwell™ 16 Tissue DNA purification kit (Promega) and total DNA concentrations were normalized to 20 ng/µl ...
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bioRxiv - Immunology 2020Quote: ... The plate reader was programmed to inject 40 μl of Luciferase Assay Substrate re-suspended in Luciferase Assay Buffer II and 35 μl of 1X Stop&Glo Reagent (Dual-Luciferase Reporter Kit, Promega) per well ...
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bioRxiv - Biophysics 2022Quote: ... Plasmids were transfected in cells (passage number < 35) seeded in 96-well plates at ~50 % confluence 2-4 days before the experiment with FuGene6 (Promega) or Lipofectamine 2000/3000 following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Oct4 was transcribed and translated in vitro in the presence of 35S-methionine (TNT Coupled Reticulocyte Lysate System, Promega, USA) according to the manufactureŕs protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... one microliter volumes of Reverse Transcription reactions were used as template for amplification of internal cDNA fragments using in 20 μl volume PCR reactions (35 rounds of amplification using Promega 2X PCR Master Mix (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... were cloned into the pSP64 in vitro translation vector and translated with a 35S radiolabelled methionine label using the TNT coupled reticulocyte lysate system (Promega) following manufacturers guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... Heterologous expression of proteins was achieved by transfecting cells with 1 □g of plasmid DNA/35 mm using FuGENE HD (Promega; E2311), as previously described (Webb et al. ...
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bioRxiv - Cell Biology 2022Quote: The other in vitro translation was done in the presence of radioactive [35S]methionine using the TNT T7 Reticulocyte Lysate System (Promega 14610). In vitro–translated proteins were then precleared by incubation with empty glutathione Sepharose beads in NETN buffer (50 mM Tris ...
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bioRxiv - Genetics 2023Quote: ... and used for thermal cycling (35× 30sec at 95°C, 55°C and 72°C) with 1X GoTaq G2 Master Mixes (Promega, cat.no.M7823), 0.2µM CTNS and 0.2µM bactin1 primers (Supplementary Table S2) ...