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Citations for Promega :
1 - 50 of 390 citations for IL 33 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 33% RRL (Promega) and 1% nanoluciferase substrate (Promega) ...
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bioRxiv - Genetics 2020Quote: ... with 33 ng pRL-SV40 (Promega) for normalization and 33 ng of transient KRAB-ZFP expression vectors (in pcDNA3.1 ...
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bioRxiv - Immunology 2021Quote: ... then with trypsin (ratio 1:33 enzyme:lysate, Promega) overnight at 35°C ...
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bioRxiv - Molecular Biology 2022Quote: ... firstly with Endoproteinase Lys-C (ratio 1:33 enzyme:lysate) (Promega) for 1 hour at room temperature then with trypsin (ratio 1:33 enzyme:lysate ...
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bioRxiv - Systems Biology 2023Quote: ... TX)33 24 hours before analysis using CellTiter-Glo viability assay (Promega, Madison, WI). Response was normalized to a DMSO control.
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bioRxiv - Biophysics 2023Quote: ... 31-33 measurements were performed by the addition of furimazine (Promega, Wisconsin, USA) at a dilution of 1:200 using a Tecan SPARK® multimode plate reader at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by an overnight trypsin digestion (ratio 1:33 enzyme:lysate, Promega, Madison, WI, USA).
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bioRxiv - Molecular Biology 2022Quote: ... for 1 hour at room temperature then with trypsin (ratio 1:33 enzyme:lysate) (Promega) overnight at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme: lysate, Promega) overnight at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... Lumit Human IL-1β Immunoassay (Promega) was performed according to manufacturer instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The eluent was equally divided into three for digestion with either: 33:1 (w/w) trypsin gold (Promega), 25:1 (w/w ...
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bioRxiv - Genetics 2023Quote: ... DNA was extracted from pools of 33-37 adults using a Wizard® genomic DNA purification kit (Promega) and a region of ∼690bp spanning the gRNA cut sites for both vas2-5958 and zpg-7280 gene drives was amplified in two fragments either side of the gene drive insert ...
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bioRxiv - Microbiology 2022Quote: ... HEV) or at 33°C (HRV) until harvested by washing in PBS and lysis in Passive Lysis Buffer (Promega). Luciferase activity was determined using either the Renilla Luciferase Assay Kit (Biotium) ...
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bioRxiv - Genomics 2020Quote: ... Total RNA was purified using hot phenol–chloroform method as described previously (33) and reverse-transcribed to cDNA using M-MLV Reverse Transcriptase (Promega). A relative real-time quantitative PCR was then carried out for all the genes studied (Table S2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Alkylated proteins were then cleaved using a two-step digestion: first with Endoproteinase Lys-C (ratio 1:33 enzyme: lysate, Promega) for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme ...
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bioRxiv - Bioengineering 2020Quote: ... Unstimulated media (+CTRL) contained complete α-MEM medium only and stimulated media (+IL-1β) contained 20 ng/mL IL-1β (FHC05510, Promega, Madison, WI, USA) in complete α-MEM medium ...
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bioRxiv - Genomics 2020Quote: ... Next nuclei were pelleted and the transposition reaction was performed incubating the lysate for 30 min at 37 °C under agitation in the presence of Transposition mixture (Tris-HCl pH 7.6 10 mM, MgCl2 5 mM, dimethyl formamide 10%, Tn5 enzyme 100 nM – Illumina #20018704, digitonin 0.01% - Promega #G9441, Tween-20 0.1%, PBS 33%). The transposition reaction was purified (Qiagen MinElute PCR Purification kit ...
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bioRxiv - Cancer Biology 2021Quote: The coding sequence of the mature IL-18 was cloned into pET-20b plasmid (Promega) before being transduced into E ...
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bioRxiv - Cell Biology 2020Quote: ... or mouse (W4021; Promega) IgGs ...
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bioRxiv - Plant Biology 2023Quote: ... or anti-mouse (Promega) in 5% low-fat milk in TBS-T for 1 hour ...
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bioRxiv - Genomics 2023Quote: ... The IL experiment RNA isolation was conducted using the Maxwell® 16 LEV plant RNA kit (Promega) (35) ...
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bioRxiv - Molecular Biology 2021Quote: ... AntiHaloTag (mouse monoclonal, Promega G9211) blot (Figure 1B ...
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bioRxiv - Cell Biology 2023Quote: ... or anti-mouse HRP (Promega) was diluted in TBST containing 5% dried nonfat milk ...
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bioRxiv - Molecular Biology 2023Quote: ... mouse anti-HaloTag (Promega, G921A), rabbit anti-DDX6 (Bethyl ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-HaloTag (Promega, G9211); rabbit anti-CIMPR (made in house) ...
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bioRxiv - Cell Biology 2023Quote: ... Halo-Tag (Promega, G9211, mouse), and HSP90 (Santa Cruz Biotechnologies ...
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bioRxiv - Physiology 2024Quote: ... or anti-mouse HRP (Promega) was diluted in TBST containing 5% dried nonfat milk ...
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bioRxiv - Immunology 2021Quote: ... IL-12 and TNFα were analyzed by semiquantitative PCR (SYBR Green assay Go Taq qPCR Master Mix; Promega) with specific primers (Metabion ...
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bioRxiv - Microbiology 2021Quote: IL-18 truncations were generated through in vitro transcription/translation using pET-pro-IL-18 as a template in 10 µl reaction volumes following the manufacturer’s recommendations (TNT Coupled Reticulocyte Lysate; Promega) as previously [40] ...
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bioRxiv - Cell Biology 2019Quote: ... α-mouse IgG HRP conjugate (Promega W4021) were used as secondary antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-βIII-tubulin (G712A; Promega), rabbit anti-Cdc42 (07-1466 ...
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bioRxiv - Plant Biology 2020Quote: ... a rabbit-anti-mouse-IgG (Promega) coupled with an alkaline phosphatase was used.
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bioRxiv - Cancer Biology 2021Quote: ... Lumit anti-mouse antibody-LgBiT (Promega) and Lumit anti-rabbit antibody-SmBiT (Promega) ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-ßIII tubulin (Promega, G7121); mouse anti-NDUFA9 (abcam ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-LacZ (1/1000; Promega), mouse anti-βPS (1/10 ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-β-galactosidase (Promega, Z378B) 1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... goat α-mouse IgG antibodies (Promega) and donkey α-goat IgG (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-Lacz (1:200)(Promega), rabbit anti-CD4 (1:250)(Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... goat α-mouse IgG antibodies (Promega) and donkey α-goat IgG (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mouse IgG HRP conjugate (Promega) anti-rabbit rhodamine–conjugated tubulin (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... anti-Halo (1:1000, mouse, Promega), anti-Myc (1:100 ...
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bioRxiv - Biochemistry 2023Quote: ... anti-mouse IgG HRP conjugate (Promega, catalog no ...
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bioRxiv - Microbiology 2023Quote: ... goat α-mouse IgG antibodies (Promega) and donkey α-goat IgG (Abcam ...
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bioRxiv - Biophysics 2023Quote: ... mouse monoclonal anti-Halo (Promega, #G9211) diluted at 1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal anti-Halo (Promega, G9211), mouse monoclonal anti-HSP90 (BD Transduction Laboratories ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-mouse HRP conjugate (Promega, W4028) and anti-rabbit HRP conjugate (Promega ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were then stimulated with 1 ng/mL of IL-1β for 6 h and subsequently washed with ice-cold PBS and lysed in Passive Lysis Buffer (PLB; Promega). Luciferase activity was measured in a Clariostar plate reader (BMG Biotech ...
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bioRxiv - Immunology 2023Quote: ... The supernatants were used to determine lactate dehydrogenase activity and IL-1β level using LDH-GloTM Cytotoxicity Assay (Promega, J2380) and Mouse IL-1 beta DuoSet ELISA (R&D Systems ...
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bioRxiv - Physiology 2022Quote: ... 1:1000 mouse anti-ATP1A1 followed by 1:2500 goat anti-mouse HRP-conjugated secondary (catalog #: W4021; Promega). p-ATP1A1Y260 and ATP1A1 bands were visualized with SuperSignal Pico utilizing the ChemiDoc.
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies were either goat anti-mouse IgG HRP or goat anti-mouse IgG HRP (Promega; 1:10,000) respectively.