Labshake search
Citations for Promega :
1 - 50 of 420 citations for IFN γ Receptor II Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... CHO-TCRact-PDL1-scarlet cells were generated by transduction of CHO-TCRact cells (Promega J1191: aAPC/CHO-K1) with virus from pLVX-PDL1-mScarlet lentiviral vector (synthesized by GenScript ...
-
bioRxiv - Immunology 2024Quote: ... 3×106 ADCC and ADCP Bioassay Effector Cells expressing the human FcγRIIIa or FcγRIIa receptors (Promega) were added per well and the cells were incubated in at 37°C with 5% CO2 for 6 hours ...
-
bioRxiv - Molecular Biology 2023Quote: ... 15 mg of His-Tev-HaloTag-(3C)-human γ-TuNA was coupled to Halo Magne beads (Promega). Xenopus laevis egg extract was prepared by standard methods28 ...
-
bioRxiv - Pathology 2022Quote: ... The infectious BSMV RNAs were transcribed in vitro from linearized plasmids γ-TaPDS, γ-TaCBS, γ-TaGSTU6, γ, α, and β (Petty et al., 1989) using the RiboMAX Large Scale RNA Production System-T7 kit (Promega, USA) and the Ribo m7G Cap Analog (Promega) ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... were co-transfected with pcDNA5/FRT construct encoding haemagglutinin (HA)-tagged human CB1 receptor cDNA and pOG44 (Flp recombinase plasmid) using transfection reagent Fugene HD (Promega) as previously described for AtT-20 pituitary tumour cells [26] ...
-
bioRxiv - Microbiology 2020Quote: ... or human IFN-β (1000 U/ml) and firefly luciferase activity was determined using the dual luciferase reporter assay system (Promega) on Cytation 3 (BioTek ...
-
bioRxiv - Neuroscience 2020Quote: ... a caspase-1 selective inhibitor Ac-YVAD-CHO (1 μM; Promega) was added in parallel experiments ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293T cells were co-transfected in a 1:1 ratio with either human D1 or D2Long receptor and a split-luciferase based cAMP biosensor (GloSensor, Promega, Durham NC). The next day ...
-
bioRxiv - Microbiology 2022Quote: The extent of ADCC activation induced by human γ-globulins was evaluated with the use of an ADCC Reporter Bioassay (Core Kit; Promega, G7010) and the EnSpireMultimode Plate Reader (PerkinElmer) ...
-
bioRxiv - Microbiology 2020Quote: ... and pGL3-IFN-λ1-Luc (IFN-λ1 luciferase reporter) were constructed by inserting the promoter region into pGL3-Basic (Promega, USA) by standard molecular cloning methods as described in our previous publications.30-32 pISRE-Luc (the luciferase reporter of ISGs ...
-
bioRxiv - Biochemistry 2022Quote: ... and M75F receptor plasmids using FuGene HD (Promega). Cells were treated with DMSO or varying drug concentrations 24 hours after transfection ...
-
bioRxiv - Microbiology 2023Quote: ... 200 ng of the IFN-β reporter plasmid (Promega, USA) and 4 ng of Renilla luciferase plasmid ...
-
bioRxiv - Immunology 2019Quote: ... CHO-K1 cells (European Collection of Cell Cultures) were stably transfected with plasmids using FuGene (Promega), stable cell lines were created ...
-
bioRxiv - Neuroscience 2019Quote: CHO-K1 cells (ATCC, cultured as described above) were transfected with rat TRAAK-GFP with FugeneHD (Promega) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 1.5 μg receptor and 1 μg GloSensor-22F (Promega). After 24 hours ...
-
bioRxiv - Immunology 2022Quote: ... CHO-K1 or HEK-293F cells (European Collection of Authenticated Cell Cultures) were transfected with plasmids using FuGene (Promega), and Fc-secreting cells cloned ...
-
bioRxiv - Cell Biology 2022Quote: ... β and γ DNA was next PCR amplified with the Q5 polymerase (Promega) according to manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: ... 24 h following stimulation with IFN cells were harvested in 1 × passive lysis buffer (Promega) and stored at −20°C ...
-
bioRxiv - Biophysics 2021Quote: ... DNA constructs of full-length wt and T654A EGFR (1 μg) were transiently transfected into CHO-K1 cells using FuGENE HD Transfection Reagent (Promega, Madison, WI). For single-molecule measurements ...
-
bioRxiv - Microbiology 2023Quote: The production of NO in response to IFN-β was quantified using a Griess assay kit (Promega) following the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2022Quote: HEK293 T cells were co-transfected with the target receptor construct and GloSensor cAMP reporter (Promega) in DMEM supplemented with 10% FBS for overnight incubation ...
-
bioRxiv - Biochemistry 2024Quote: lrl 1μg of N-terminally FLAG-tagged receptor and 1μg of F22 (Promega, Cat. no: E2301) (for GloSensor assay)
-
bioRxiv - Molecular Biology 2020Quote: ... Using 100 ml of Luciferase Assay Reagent II (LAR II) (Luciferase Assay Reagent, Promega) and ...
-
bioRxiv - Microbiology 2021Quote: ... ImProm-II Reverse Transcriptase (Promega) was used to synthesize cDNA from total RNA ...
-
bioRxiv - Molecular Biology 2021Quote: ... was digested with the same enzymes and ligated with the amplified receptors using T4 DNA ligase (Promega).
-
bioRxiv - Immunology 2021Quote: ... Relative levels of IFNλs were estimated using the extracellular HiBiT split luciferase assay by virtue of their C-terminal HiBiT tag by incubating IFN preparations with assay reagents and measured by manufacturer’s instructions (Nano-Glo HiBiT Extracellular Detection system, Promega) using a luminometer.
-
bioRxiv - Biochemistry 2021Quote: ... HEK-293 cells were transfected with FLAG-tagged receptor (3.5 μg) and luciferase-based 22F cAMP biosensor construct (3.5 μg) (Promega). 14–16 h post transfection ...
-
bioRxiv - Cell Biology 2024Quote: ... HaloTag-receptor expressing mESCs were fluorescently labeled with either 1 µM of HaloTag AlexaFluor 488 ligand (Promega, G1001) or 3.5µM of HaloTag AlexaFluor 660 ligand (Promega ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: To measure ATP bound to detergent purified P2X1 receptor a Kinase-Glo® Luminescent Kinase Assay (ProMega, USA) was used ...
-
bioRxiv - Microbiology 2019Quote: ... and ImProm-II Reverse Transcriptase (Promega) was performed31 ...
-
bioRxiv - Microbiology 2021Quote: ... and Improm-II reverse transcriptase (Promega) according to the supplier’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... and ImProm II reverse transcriptase (Promega). Quantitative RT-PCR was performed using a Thermal Cycler Dice Real Time System II (Takara Bio Inc. ...
-
bioRxiv - Microbiology 2020Quote: ... post-transfection with or without OSM (10 ng/mL for 8 h) or IFN-α (1,000 U/ml for 16 hours) before lysis using Passive Lysis Buffer (Promega). Firefly and Renilla luciferase activity was then determined in a dual luciferase assay ...
-
bioRxiv - Neuroscience 2020Quote: ... The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (1.764 µg) were also transfected into HEK293T cells cultured on acid-washed coverslips using FuGENE HD (Promega) in Optimem (Gibco ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with 2 μg of FLAG-tagged β2 or β1 adrenergic receptor DNA using Fugene 6 (Promega) and seeded at a density of ∼70,000 cells.ml−1 on 18 mm glass coverslips 4-8 hours after transfection.
-
bioRxiv - Biophysics 2023Quote: ... HEK293T cells were co-transfected with FLAG-tagged receptor (3.5μg) and luciferase-based 22F cAMP biosensor construct (3.5μg) (Promega, Cat. no: E2301) using polyethyleneimine (PEI ...
-
bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
-
bioRxiv - Neuroscience 2020Quote: ... All designed probes were tested by ddPCR using human genomic DNA (Human male, Promega) as a negative control ...
-
bioRxiv - Biochemistry 2021Quote: ... WT or ADAR1-KO HEK293T cells in 12-well plates were transfected with 400 ng ml-1 of firefly luciferase under control of the IFN-β promoter (pIFN-Luc, Promega), 40 ng ml-1 of Renilla luciferase under a constitutive promoter (pRL-TK ...
-
bioRxiv - Microbiology 2020Quote: ... transfection experiments) or 4×105 cells (12 well format; IFN experiments) were lysed in 120 μl passive lysis buffer (Promega #E1941). Half the lysate was used to quantify luciferase protein (Promega Luciferase Assay System #E1501 ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were treated 8 h post-transfection with or without IFN-α (1000 U/ml) before lysis 16 h later using Passive Lysis Buffer (Promega). Firefly and Renilla luciferase activity was then determined in a dual luciferase assay ...
-
bioRxiv - Cancer Biology 2023Quote: ... at 3,000 cells/well in 50 μL media containing IFN-α for 72 h prior to cell lysis with 50 μL CellTiterGlo 2.0 (Promega, G9243). The reaction was incubated in the dark for 20 min at RT ...
-
bioRxiv - Molecular Biology 2022Quote: ... Human Genomic DNA (G1521, Promega) was used as positive control and synthetic DNA was used to validate the LAMP assay performance ...
-
bioRxiv - Neuroscience 2021Quote: ... or with ImProm-II reverse transcriptase (Promega). Real time qPCR was performed in duplex with Actin or Ubiquitin C as a reference gene ...
-
bioRxiv - Microbiology 2021Quote: ... The ImProm-II reverse transcriptase system (Promega) was used with random hexamers and 5 µL of extracted RNA to synthesize cDNA ...
-
bioRxiv - Microbiology 2022Quote: ... with the ImProm-II reverse transcriptase (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... The ImProm-II reverse transcription kit (Promega) was used to synthesize cDNA from 1 µg of total RNA using the included oligo dT primers in a volume of 20 µL per the manufacturer’s direction ...
-
bioRxiv - Biochemistry 2023Quote: ... and ImProm-II Reverse Transcriptase (Promega, #A3802) were used ...
-
bioRxiv - Biophysics 2021Quote: ... sapiens TACAN-eGFP or the M2 muscarinic receptor (Halo-M2R-eGFP) plasmid (previously established in this lab) was transfected into the cells using FugeneHD (Promega) following the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2022Quote: ... HEK293 cells were co-transfected with FLAG-tagged receptor constructs and luciferase-based 22F cAMP biosensor (Promega, Cat. no. E2301) using polyethylenimine (PEI ...