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Citations for Promega :
1 - 50 of 3815 citations for Human Tropomyosin alpha 4 chain TPM4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Polymerase chain reaction (PCR) was performed using GoTaq kit (Promega, Madison, WI) unless otherwise indicated.
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... Polymerase chain reaction (PCR) was performed using GoTaq kit (Promega, Madison, WI).
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bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
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bioRxiv - Microbiology 2021Quote: ... knowlesi elongation factor-1 alpha (pkef1-alpha) 5’ UTR were isolated from agarose gels and cloned into the pGEM-T vector (Promega, Madison, WI, USA). To replace the pfcam 5’ UTR sequence that drives luciferase transcription in the pD-pfcam-Luc ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Polymerase chain reactions were performed using 2 U Gotaq (Promega), 1X Gotaq buffer (Promega) ...
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bioRxiv - Genetics 2021Quote: ... All other polymerase chain reactions (PCR) were performed using the GoTaq (Promega) enzyme system as per manufacturer instructions (95°C for 2 min ...
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bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
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bioRxiv - Biochemistry 2023Quote: ... Bovine alpha-2-hs-glycoprotein was isolated from plasma (Fetuin, Promega, V4961), and monocyte differentiation antigen CD14 was recombinantly expressed in CHO cells (R&D systems ...
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bioRxiv - Neuroscience 2020Quote: ... and inserting epitope tags via polymerase chain reaction with Pfu DNA polymerase (Promega). Primers for HA insertion into pIRES2- EGFP-FL ...
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bioRxiv - Zoology 2021Quote: ... Polymerase chain reactions (PCR) were carried out using GoTaq Green Master Mix (Promega) in the following conditions ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative polymerase chain reaction (qPCR) was performed with the GoTaq qPCR Mastermix (Promega) and QuantStudio 5 Real Time PCR (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... Real time-polymerase chain reactions were performed with GoTaq qPCR Master Mix (Promega) according to the manufacturer’s instructions and completed on QuantStudio 6 Flex system (ABI) ...
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bioRxiv - Microbiology 2022Quote: Polymerase chain reaction was performed using the GoTaq PCR master mix (Promega, USA) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Triplicate biological replicates were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described68 ...
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bioRxiv - Cancer Biology 2022Quote: ... Quadruplicate biological replicates were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described 60 ...
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bioRxiv - Plant Biology 2021Quote: ... http://cshprotocols.cshlp.org/content/2009/2/pdb.prot5110.abstract) with trypsin (porcine, side chain protected; Promega). Briefly ...
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bioRxiv - Biophysics 2023Quote: ... followed by polymerase chain reaction (PCR) with GoTaq® G2 Master Mix (M7822, Promega) on Biometra TRIO Touch Thermocycler (207072X ...
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bioRxiv - Cell Biology 2024Quote: ... biological replicates (n=7) were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described (Hubner et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were resolved in reducing and non-reducing SDS-PAGE gels and membranes were incubated overnight at 4°C with the following antibodies: rabbit polyclonal anti-human p75 intracellular domain (1:1000, Promega); mouse monoclonal anti-HA (1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... Baseline fluorescence was measured in an ELISA reader (Promega) following an incubation of 30 min at 37°C ...
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bioRxiv - Biophysics 2022Quote: ... The variable and CH1 regions of the NbFab heavy chain were cloned into pTarget (Promega) following a N-terminal (MRPTWAWWLFLVLLLALWAPARG ...
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bioRxiv - Cancer Biology 2020Quote: ... Human cell lines were DNA fingerprinted for provenance using the Power-Plex 1.2 kit (Promega) and confirmed to be the same as the DNA fingerprint library maintained by ATCC ...
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bioRxiv - Biochemistry 2019Quote: ... a polymerase chain reaction using Pfu DNA polymerase (Thermo Fischer Scientific) or Pfu DNA polymerase (Promega), the template pNZ-SV10HnisT or pIL-SVnisT and the primer pair nisTH551Afor and nisTH551Arev (Supplementary File 4 ...
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bioRxiv - Immunology 2020Quote: ... Gel bands for heavy and light chains were excised and in-gel digested using trypsin (Promega), chymotrypsin (Promega) ...
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bioRxiv - Genetics 2020Quote: ... Cell growth after 4 days was measured using the CellTiterGlo 2 kit (Promega) and a GloMax luminometer (Promega) ...
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bioRxiv - Immunology 2023Quote: ... Three biological replicates for each condition were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) “on beads” as previously described [44] ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 24-48hrs in the blocking solution containing chicken anti-human NT-4/5 antibody (10 mg/ ml, Promega, Madison, WI) or rabbit anti-human NT-4/5 (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: Human Genomic DNA was extracted from the blood using the Wizard Genomic DNA Purification kit (Promega) as per the instructions.
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bioRxiv - Neuroscience 2021Quote: Genomic DNA (gDNA) was extracted from human frontal cortex using Wizard Genomic DNA Purification Kit (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... All DNA handles were made by polymerase chain reaction (PCR) using λ DNA cl857 Sam7 (Promega, D1501) as a template and a forward primer containing two digoxigenin labels at the 5’ end ...
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bioRxiv - Cancer Biology 2021Quote: ... Biological triplicates were digested with Lys-C (Alpha Laboratories, Eastleigh, UK) and trypsin (Promega, Madison, WI, USA) on beads as previously described (56) ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative real-time polymerase chain reaction (PCR) was performed for CGRP with GoTaq® qPCR Master Mix (Promega) using the DNA-binding dye ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK cells were transfected with both heavy and light chain BCR constructs using FuGENE HD transfection reagent (Promega) in a 1:3 ratio ...
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bioRxiv - Biophysics 2023Quote: ... ELISA plates were read on a GloMax plate reader (Promega, Wisconsin, US). Data were converted from concentration to total mass based on the volume of media/pellet ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was stored at −20°C until analysed by polymerase chain reaction using GoTaq®G2 DNA polymerase (Promega) according to the manufacturers protocol ...
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bioRxiv - Immunology 2021Quote: ... Confirmed heavy and light chain plasmid DNA were co-transfected in 293T cells (ATCC) using Fugene transfection reagent (Promega) in 24 well plates for preparing antibody supernatant for initial screening for their expression and antigen specificity as detailed in the following section ...
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
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bioRxiv - Neuroscience 2020Quote: ... All designed probes were tested by ddPCR using human genomic DNA (Human male, Promega) as a negative control ...
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bioRxiv - Molecular Biology 2022Quote: ... Human Genomic DNA (G1521, Promega) was used as positive control and synthetic DNA was used to validate the LAMP assay performance ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times in TBST for 5 minutes and further incubated with HRP-conjugated secondary anti-mouse-IgG-H&L chain (Promega) or anti-rabbit-IgG-F(ab’)2 (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... and real-time polymerase chain reaction (RT-PCR) conducted using the Techne Prime Pro thermal cycler with GoTaq qPCR Master Mix (Promega). RT-qPCR reactions were carried out in 10 μL volumes containing 3.5 mM MgCl2 ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times in TBST for 5 minutes and were then incubated with HRP-conjugated secondary anti-mouse-IgG-H&L chain (Promega) or anti-rabbit-IgG-F(ab’)2 (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: All isolates were heat-extracted at 95°C for 20 minutes for polymerase chain reaction (PCR) using GoTaq (Promega M7132) and 8F and 1492R primers to amplify the whole 16S rRNA gene (4) ...
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bioRxiv - Neuroscience 2019Quote: ... The quality of the cDNA was tested using GPDH primers (see below for sequence) and GoTaq endpoint polymerase chain reaction (PCR) protocol (Promega). Quantitative PCR was performed using a SYBR Green protocol (Applied Biosystems ...
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bioRxiv - Zoology 2021Quote: ... Polymerase chain reactions in a 15 μL solution containing 7.5 μL GoTaq® G2 Hot Start Green Master Mix (Promega), 0.4 μmol of each primer and 46 – 247 ng of genomic DNA were performed using a C1000 Touch Thermal Cylinder (BioRad) ...
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bioRxiv - Evolutionary Biology 2020Quote: To generate the pGl3-STK38-P luciferase reporter,1.2 kb of DNA upstream the STK38 TTS promoter region with ZNF611 binding motif was amplified by polymerase chain reaction (PCR) and cloned into the Firefly luciferase reporter plasmid pGL3-Basic (Promega) using KPN1 and XHO1 restriction sites.0.7 kb of DNA upstream the STK38 TTS promoter region without ZNF611 binding motif was cloned into pGL3-Basic using same restriction sites to generate the pGl3-STK38-ΔP luciferase reporter ...
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bioRxiv - Molecular Biology 2023Quote: ... was removed by evaporation using speed-vac and the residue was used to produce L-[2,3,4,5-3H]-proline labeled procollagen chains and the collagen-like proteins in the TnT® Coupled Reticulocyte Lysate Systems (Promega) using either SP6 or T7 promoters ...
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bioRxiv - Microbiology 2020Quote: ... pH 8.0 using Vivaspin columns (3 kDa) and digested separately overnight using trypsin chymotrypsin or alpha lytic protease (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...
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bioRxiv - Molecular Biology 2022Quote: ... sequence was amplified from human genomic DNAs and used as in vitro transcription template in the reaction using Riboprobe System-T7 Kit (P1440, Promega) in the presence of 0.4μL 5-(3-Aminoallyl)-uridine-5’-triphosphate labeled with ATTO 680(Aminoallyl-UTP-ATTO-680 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The 25 µL polymerase chain reaction (PCR) mixture was the same for both mtDNA genes using 5x Green GoTaq® Flexi buffer (Promega), 0.2 mM dNTPs ...