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Citations for Promega :
1 - 50 of 1480 citations for Human RPP25L shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and 1 μg shRNA plasmid using Fugene 6 (Promega). The next day ...
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bioRxiv - Neuroscience 2019Quote: ... HEK293T cells were transfected with control or shRNA-containing pFUGW (GFP-expressing) vector plasmids and helper plasmids PAX2 and VSVG using Fugene (Promega). Supernatant was collected 65 hr after transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... pLKO shRNA-DNA was transfected with psPAX2 packaging and pMD2.G envelop plasmid using Fugene reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: The plasmid pEGFP-C1-human Rab29 was generated by inserting human Rab29 sequence from pFN21A-Halo-Rab29 (Promega, #FHC08084) into Bgl II - Eco RI site of pEGFP-C1-rat Rab29 plasmid that was used previously11 ...
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bioRxiv - Cancer Biology 2023Quote: HEK293T cells were transfected with a FLAG-tagged human DHHC20-expressing plasmid and a corresponding HA-tagged substrate-expressing plasmid using FuGENE transfection reagent (Promega) and incubated overnight ...
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bioRxiv - Cell Biology 2023Quote: ... and 10μg of pLKO.1-mCherry-scrambled shRNA or pLKO.1-mCherry-IRE1α shRNA using FuGENE-HD reagent (Promega, Madison, WI, USA). After 24 h of transfection ...
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bioRxiv - Pathology 2019Quote: ... human primary aortic SMCs were transfected with pGL4.34 Vector plasmids (E1350; Promega, Madison, WI) using Effectene Transfection Reagent (301425 ...
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bioRxiv - Genetics 2022Quote: ... human POPDC1 and POPDC2 cDNA sequences were cloned into the pFC14K or pFC32K plasmids (Promega), which contain C-terminus sequences for HaloTag and NanoLuc tags ...
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bioRxiv - Neuroscience 2020Quote: ... and pCMVΔ8.9 plasmids were co-introduced into human embryonic kidney 293T cells using the FuGENE reagent (Promega). 48 h after transfection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were transfected with human RXFP1 pcDNA-Zeo-tetO and the GloSensor reporter plasmid using FuGENE (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with a control Flag or HA-GSK-3βWT human plasmid using Fugene 6 (Promega #E2693) in a 2:1 ratio and incubated for 3 hours in a CO2 incubator ...
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bioRxiv - Genomics 2022Quote: ADGRG6_3 and ADGRG_4 sequences were PCR amplified from human genomic DNA and cloned into the pGL4.23 plasmid (Promega, E8411). Human preadipocytes ...
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bioRxiv - Neuroscience 2021Quote: We constructed luciferase reporter plasmids by cloning an ∼900 bp region containing human 1b30 into the pGL4.24 vector (Promega) upstream of the minP ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were co-transfected with pcDNA5/FRT construct encoding haemagglutinin (HA)-tagged human CB1 receptor cDNA and pOG44 (Flp recombinase plasmid) using transfection reagent Fugene HD (Promega) as previously described for AtT-20 pituitary tumour cells [26] ...
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bioRxiv - Neuroscience 2022Quote: The scATAC-seq peaks that overlapped obesity-associated SNPs were PCR amplified from human genomic DNA (see primers in Supplementary Table 2) and cloned into the pGL4.23 plasmid (Promega, E84111). The associated SNPs were then introduced into these plasmids by PCR amplification with primers containing the associated variants ...
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bioRxiv - Cancer Biology 2021Quote: ... shRNA constructs were packaged in HEK 293T cells using FuGENE 6 Transfection Reagent (Promega E2691) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... standard curves were generated by preparing 10 fold dilution series of plasmid DNA containing parasite 18SrRNA gene54 and from human genomic DNA (Cat No. G304, Promega, Australia). Thermal cycling was performed on a Light Cycler 480 II (Roche ...
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bioRxiv - Microbiology 2022Quote: 0.5mg of plasmid DNA (purified pAdvantage plasmid, Promega), 1mg of bacterial genomic DNA (isolated from E ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293T cells were seeded in 10-cm cell culture dishes and transfected at ∼ 80% confluency with a packaging plasmid mixture (transgene plasmid; VSVG plasmid; PAX2 plasmid) using FuGENE HD transfection reagent (Promega, E2311). Twenty-four hours after transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... Standard curves for each primer set were generated using serially diluted plasmid (for gAAV) or Human and Mouse Genomic DNA (for host) (Promega G1521 and G3091, respectively) and used for quantification ...
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bioRxiv - Physiology 2020Quote: ... Plasmids were isolated using PureYield Plasmid Miniprep System (Promega) and sanger sequencing was performed (GATC Biotech AG ...
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bioRxiv - Synthetic Biology 2020Quote: ... gRNA plasmids and base-editor plasmids were purified using the PureYield Plasmid Miniprep System (Promega, A1223) and Plasmid Plus Maxi Kit (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... plasmids were extracted (PureYield Plasmid Midiprep System, Promega, Maddison, USA) and correct sequence of the shRNA was controlled with sanger sequencing (MWG Eurofins Genomics) ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasmids were isolated using the PureYield Plasmid Miniprep System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid DNA was extracted using PureYieldTM plasmid miniprep system (Promega). PCR products were purified using GFXTM PCR DNA and Gel Band Purification Kit (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... cAMP-dependent GloSensor reporter plasmid (−22F cAMP plasmid, Promega, E2301), and Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... 0.4 μg envelope vector (pMD2.G) and 1.6 μg hairpin-pLKO.1 vector (SHC016 control or gene specific shRNA. Fugene-6 (Promega) was used as transfection reagent ...
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bioRxiv - Immunology 2019Quote: ... 0.4 µg envelope vector (pMD2.G) and 1.6 µg hairpin-pLKO.1 vector (SHC016 control or gene specific shRNA. Fugene-6 (Promega) was used as transfection reagent ...
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bioRxiv - Microbiology 2019Quote: pSP72 plasmids (Promega) encoding the full-length genomic cDNA of HMPV B2/CAN98-75 or A1/C-85473 strains (GenBank accession numbers ...
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bioRxiv - Bioengineering 2021Quote: ... The plasmid was extracted with PureYield™ Plasmid Miniprep System (Promega) and quantified on a Quantus™ Fluorometer using the QuantiFluor(R ...
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bioRxiv - Genomics 2021Quote: ... with a firefly plasmid and the pRL-CMV plasmid (Promega, # E2261) containing the Renilla luciferase gene for normalization ...
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bioRxiv - Microbiology 2022Quote: ... plasmids were then isolated using the PureYield Plasmid Midiprep kit (Promega), and sequenced to confirm mutations ...
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bioRxiv - Molecular Biology 2019Quote: ... Plasmid DNA was isolated using PureYield™ plasmid miniprep system (Promega), according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... psPAX2 (packaging plasmid) and pMD2.G (envelope plasmid) using FuGENE (Promega) or Lipofectamine3000 (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmid purification was performed using the PureYieldTM Plasmid Miniprep System (Promega). Concentration and purity of DNA were checked on NanoDrop ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmid purification was performed using the PureYieldTM Plasmid Miniprep System (Promega). Concentration and purity of DNA were checked on NanoDrop ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA was isolated using Promega PureYield Plasmid Miniprep System (Promega) and sent to Genewiz (South Plainfield ...
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bioRxiv - Synthetic Biology 2023Quote: ... and plasmids were isolated using a PureYield Plasmid Mediprep kit (Promega).
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bioRxiv - Cancer Biology 2020Quote: ... viruses were generated in HEK 293T cells transfected with lentiviral packaging vectors along with vectors expressing pGIPZ-shRNA using Fugene6 (Promega). Two distinct hairpins were chosen for the experiments ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were transfected with packaging vectors pMD2.G and psPAX2 along with relevant shRNA using Fugene 6 transfection reagent (Promega). Viral particles were harvested after 48 hours in DEMEM/ 40% FBS ...
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bioRxiv - Biophysics 2020Quote: ... The plasmids were purified using the PureYield™ Plasmid Miniprep System (Promega).
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bioRxiv - Molecular Biology 2022Quote: ... Each plasmid wasprepared using plasmid DNA isolation kit (Promega, Cat No. # A1222) and 100 ng working stocks were prepared ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we extracted the plasmid using the PureYield Plasmid Midiprep System kit (Promega) and linearized it by restriction digest ...
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bioRxiv - Genetics 2020Quote: ... The firefly luciferase reporter plasmids and renilla luciferase control plasmid (Promega, E2241) were co-transfected into HepG2 cells with Lipoplus (Sagecreation ...
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bioRxiv - Microbiology 2019Quote: ... Plasmid purification was performed using PureYield ™ Plasmid Miniprep system kit (Promega) following instructions of the provider ...
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bioRxiv - Microbiology 2023Quote: The pABA plasmid was purified using PureYield™ Plasmid Miniprep System (Promega), starting with 5 mL of an overnight grown culture ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmids were isolated from bacterial cells using PureYield Plasmid Miniprep System (Promega) or QIAprep Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2021Quote: ... pRL-TK plasmid (Promega) expressing Renilla luciferase (internal control) ...
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Herpes simplex virus 1 entry glycoproteins form stable complexes prior to and during membrane fusionbioRxiv - Microbiology 2022Quote: ... All plasmids from Promega contained an HSV-TK promoter ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmid pRL-TK (Promega) was kindly provided by Dr ...