Labshake search
Citations for Promega :
1 - 50 of 311 citations for Human Ephrin B2 EFNB2 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2023Quote: ... His-tag proteins have been purified by using MagneHis system (Promega) and DynaMag spin magnet (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2022Quote: ... supernatant was collected and purification was done via His-tag using HisLink Protein Purification Resin (V8823, Promega) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... Rat Gβ1 was fused with a His-tag at the N terminus and with a SmBiT subunit (peptide 86, Promega)34 after a 15-amino acid linker at its C terminus ...
-
bioRxiv - Evolutionary Biology 2022Quote: Plasmid and B2 insert were ligated together using a rapid ligation kit (Promega). Ligation reactions contained 2.5µl 4x buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... 15 mg of His-Tev-HaloTag-(3C)-human γ-TuNA was coupled to Halo Magne beads (Promega). Xenopus laevis egg extract was prepared by standard methods28 ...
-
bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.5 µg (HEK293A, HEK293T, HEK293AΔARRB1/B2) or 2 µg (HEK293AΔGs, HEKS293AΔGi) Glo-22F cAMP plasmid DNA (Promega) and 2 µg MOCK DNA (HEK293A ...
-
bioRxiv - Microbiology 2021Quote: ... anti-Halo-tag (Promega), anti-FLAG (M2 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Halo-Tag from Promega, USA (G9211) ...
-
bioRxiv - Genomics 2019Quote: To generate recombinant p53 we in vitro transcribed/translated human p53 with a c-terminal HA tag using a rabbit reticulocyte system (Promega). To generate fragmented genomic DNA we tagmented 50ng of human genomic DNA from MCF7 cells using the MuSeq kit (Thermo ...
-
bioRxiv - Biochemistry 2022Quote: ... 1200 ng of ORs tagged with the first 20 amino acids of human rhodopsin (rho-tag) at the N-terminal ends53 in pCI (Promega) and 30 ng eGFP were transfected using Lipofectamine 2000 (Invitrogen ...
-
bioRxiv - Neuroscience 2023Quote: ... 1,200 ng of ORs tagged with the first 20 amino acids of human rhodopsin (rho-tag) at the N-terminal ends in pCI mammalian expression vector (Promega) and 30 ng eGFP were transfected using Lipofectamine 2000 (11668019 ...
-
bioRxiv - Cell Biology 2023Quote: ... Halo-Tag (Promega, G9211, mouse), and HSP90 (Santa Cruz Biotechnologies ...
-
bioRxiv - Plant Biology 2024Quote: ... Magne Halo Tag Beads (Promega) were used to purify and capture the produced protein ...
-
bioRxiv - Microbiology 2021Quote: ... n=4 vaginal) and rUTI (n=3 urine) cohorts belonging to B2 phylogenetic group was extracted (Wizard Genomic DNA Purification Kit, PROMEGA) and sequenced by Illumina NovaSeq 2×150 nt ...
-
Multimodal tubulin binding by the yeast kinesin-8, Kip3, underlies its motility and depolymerizationbioRxiv - Biophysics 2021Quote: ... An N-terminal HALO tag (Promega) for fluorescent labelling was fused to the coding sequence through a GGSGGSLQ linker ...
-
bioRxiv - Cell Biology 2022Quote: ... into pHTN Halo-Tag vector (Promega). mCherry-WT-p62 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Halo Tag Alexa Fluor 660 (Promega) was used ...
-
bioRxiv - Microbiology 2020Quote: ... the cells were transiently transfected with pUC57 constructs harboring 1.3-mer HBV genomes (genotypes A2, B2, C2, and D3) using FuGene 6 transfection reagent according to the manufacturer’s instructions (Promega, WI, USA). The generation of plasmids has been previously described and this transient expression system relies on the endogenous promoters of HBV for transcription (6) ...
-
bioRxiv - Immunology 2021Quote: ... and HHEX (NM_002729) attached to a FLAG tag at the 5’ end and HiBit tag (Promega, Madison, WI) at the 3’ end ...
-
bioRxiv - Biophysics 2021Quote: ... and Halo-Tag (O4) ligand (Promega, P6741). To avoid non-specific interaction the glass chambers were washed with blocking buffer (20 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2021Quote: ... JF646-conjugated Halo Tag ligand (Promega, GA1120) was added to culture at a final concentration of 200 nM and cells were incubated for 30 mins before live-imaging ...
-
bioRxiv - Biophysics 2022Quote: ... Then Halo-Tag (O4) ligand (Promega, P6741) were passed and incubated overnight at room temperature ...
-
bioRxiv - Bioengineering 2019Quote: ... Halo Tag Alexa Fluor 488 Ligand (Promega) was diluted with moderate amount of PBS ...
-
bioRxiv - Microbiology 2023Quote: ... Mouse anti-HiBiT tag mAb (30E5, Promega) and goat anti-mouse IgG H&L conjugated with Alexa Fluor 568 (Abcam ...
-
bioRxiv - Biochemistry 2023Quote: ... with the C-terminal HiBiT-Tag (Promega), using the In-Fusion cloning kit ...
-
bioRxiv - Microbiology 2023Quote: ... or the Magne-His purification system (Promega Corporation) for PlzARD-RD per manufacturer protocol ...
-
bioRxiv - Cancer Biology 2024Quote: ... were cloned into pcDNA3.1-myc-His plasmid (Promega). For sequencing we used a sequence analyser (ABI Prism 3100 Avant ...
-
bioRxiv - Molecular Biology 2020Quote: ... Recombinant Halo-tag PUM1 RBD (aa 828-1176) and Halo-tag PUM2 RBD (aa 705-1050) were expressed from plasmid pFN18A (Promega) in KRX E ...
-
bioRxiv - Cell Biology 2021Quote: ... with that of the Halo-tag protein (Promega), with the insertion of a 45-base linker (15 amino acids ...
-
bioRxiv - Cancer Biology 2022Quote: ... Halo tag TMR Direct Ligand (Promega, cat # G2991) was used for staining of Halo tag transfected fixed cells ...
-
bioRxiv - Cancer Biology 2022Quote: ... Halo-tag (G9211) antibody was purchased from Promega. The C11orf53 antibody was produced in rabbit in house by using full-length C11orf53 recombinant protein as antigen.
-
bioRxiv - Molecular Biology 2023Quote: ... rabbit polyclonal anti-Halo Tag antibody (G928A; Promega), and mouse monoclonal anti-GAPDH antibody (M171-3 ...
-
bioRxiv - Molecular Biology 2024Quote: ... with Halo-Tag TMRDirect ligand (Promega, WI, USA), as per the manufacturer’s guidelines ...
-
bioRxiv - Biochemistry 2021Quote: ... which contains a pRSET backbone with an N-terminal 6xHis tag and the insertion of a biotin tag from the PinPoint™ Xa-1 Vector (Promega, USA) in between the His tag and the first fluorescent protein ...
-
Cytoplasm localized ARID1B promotes oncogenesis in pancreatic cancer by activating RAF-ERK signalingbioRxiv - Cell Biology 2019Quote: Halo tag (Halo pulldown and labeling kit, Promega Corporations) and Streptavidin bead (GE Health care Bio-Science ...
-
bioRxiv - Cell Biology 2021Quote: ... with that of the Halo 7-tag protein (Promega), with the insertion of a 63-base linker (21 amino acids ...
-
bioRxiv - Zoology 2021Quote: ... 10μL Go-tag qPCR Master Mix (Promega, Wisconsin, USA), 0.2μL of forward primer ...
-
bioRxiv - Cell Biology 2021Quote: ... To detect HALO tag 0.5µM Janelia Fluor 549 (Promega) dye or HaloTag® TMR Ligand (Promega ...
-
bioRxiv - Biophysics 2023Quote: ... followed by Halo-Tag amine (O4) ligand (Promega, P6741). The chambers were then treated with a blocking buffer (20 mM Tris-HCl of pH 7.4 ...
-
bioRxiv - Microbiology 2019Quote: ... the His-VqmA was purified using MagneHis Ni-Particles (Promega).
-
bioRxiv - Molecular Biology 2022Quote: ... His-tagged proteins were purified using Ni-NTA resin (Promega); bound proteins were washed with binding buffer and eluted into elution buffer (4 M urea ...
-
bioRxiv - Cell Biology 2024Quote: ... HIS-PLK1 used in ADP-Glo was from Promega (V2841). HIS-PLK1 used in Fig ...
-
bioRxiv - Cell Biology 2021Quote: ... The Halo-Tag or NanoLuc genes were acquired from Promega and were genetically fused to the respective N- or C-termini.
-
bioRxiv - Biochemistry 2023Quote: Halo tags were stained with HaloTag® Ligands TMRDirect (Promega), and SNAP tags were stained with SNAP-Cell® 505-Star (NEB) ...
-
bioRxiv - Plant Biology 2020Quote: ... Magne® Halo Tag® Beads (Promega, USA, Catalog No G728A) were used to purify recombinant proteins according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... JF-549 or Halo-tag antibody (1:500, Promega, mouse monoclonal) and measuring the newly synthesized actin (in most cases ...
-
bioRxiv - Biochemistry 2020Quote: ... The plasmid expressing the Halo tag (pFN21A) was purchased from Promega. The expression plasmids His12_PreScission _C1_C2B_MUN_C2C_tev_Halo and His12_PreScission _C1_C2B_MUN_tev_Halo (ΔC2C Munc13 ...
-
bioRxiv - Plant Biology 2021Quote: ... Expressed proteins were directly captured using Magne Halo Tag Beads (Promega). The protein-bound beads were incubated with adaptor-ligated gDNA fragments ...
-
bioRxiv - Molecular Biology 2020Quote: ... Halo-tag containing proteins were purified using Magnetic HaloLink Resin (Promega) at 4°C ...