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Citations for Promega :
1 - 50 of 161 citations for Ebola Virus Envelope Glycoprotein GP1 Bundibugyo since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and 0.25 μg vesicular stomatitis virus G glycoprotein expression plasmids using the Fugene 6 transfection reagent (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2024Quote: ... Virus was concentrated using PEG virus precipitation (Promega # V3011). In brief ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... an envelope plasmid encoding VSV-G and pAdVAntage (Promega). Four days after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Bovine alpha-2-hs-glycoprotein was isolated from plasma (Fetuin, Promega, V4961), and monocyte differentiation antigen CD14 was recombinantly expressed in CHO cells (R&D systems ...
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bioRxiv - Cancer Biology 2021Quote: ... psPAX2 (packaging plasmid) and pMD2.G (envelope plasmid) using FuGENE (Promega) or Lipofectamine3000 (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... and envelope plasmid using calcium phosphate-mediated transfection method (Promega, E1200) and harvesting the supernatant 48 - 72 h after transfection ...
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bioRxiv - Microbiology 2023Quote: ... and 0.25 μg VSV-G envelope plasmid using Fugene 6 (Promega) transfection reagent ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.2 μg of pMD2.G envelope plasmid using FuGENE 6 (Promega). Cells were incubated up to 48 hrs and then lentivirus-containing media was collected and concentrated with Lenti-X concentrator (Clontech) ...
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bioRxiv - Molecular Biology 2023Quote: ... the psPax2 envelope and the vsv-G packaging plasmids using Fugene6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and 1 μg of a plasmid expressing the VSV G glycoprotein using the FuGene HD reagent (Promega). Forty-eight hours later ...
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bioRxiv - Biophysics 2020Quote: ... Virus infectivity was determined by luciferase production (Promega) after 48h using a Synergy2 Multi-Detection Microplate Reader (BioTek)
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bioRxiv - Microbiology 2020Quote: ... virus replication was measured by nLUC assay (Promega) using a SpectraMax plate reader (Molecular Devices).
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bioRxiv - Immunology 2020Quote: ... using Moloney murine leukemia virus reverse transcriptase (Promega) according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2021Quote: ... conducted with Moloney murine leukemia virus reverse transcriptase (Promega) and random hexamers (Roche Applied Science ...
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bioRxiv - Neuroscience 2023Quote: ... and Moloney murine leukemia virus MMLV reverse transcriptase (Promega) was performed according to the vendor’s protocol.
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bioRxiv - Bioengineering 2022Quote: ... Virus growth was measured with NanoGlo Luciferase Assay System (Promega) and cell viability was measured with CellTiter-Glo 2.0 Cell Viability Assay (Promega) ...
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bioRxiv - Microbiology 2019Quote: ... 200 U of Moloney murine leukemia virus reverse transcriptase (MMLV; Promega) in 30 mM Tris and 20 mM KCl (pH 8⋅3 ...
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bioRxiv - Cell Biology 2020Quote: ... Virus DNA liposomes were prepared using FuGENE HD Transfection reagent (Promega) and DMEM ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 unit/μl Avian Myoblastosis virus reverse transcriptase (Promega®) were added and incubated for 1h at 30°C ...
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bioRxiv - Microbiology 2023Quote: ... Virus infectivity was measured by β-galactosidase activity (Promega, Madison, WI). Virus stocks were concentrated (20X ...
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bioRxiv - Microbiology 2023Quote: Virus neutralization was measured using a β-galactosidase-based assay (Promega) with TZM.bl target cells (85) ...
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bioRxiv - Cell Biology 2023Quote: ... and reverse-transcribed using Moloney murine leukemia virus reverse transcriptase (Promega). cDNA was amplified in a thermal cycler (GeneAmp PCR System 9700 ...
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bioRxiv - Cancer Biology 2024Quote: ... Moloney Murine Leukemia Virus Reverse Transcriptase RNase H-Point Mutant (Promega) was used for cDNA synthesis ...
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bioRxiv - Microbiology 2019Quote: ... 625 ng of lentiviral packaging vector psPAX and 625 ng of envelope vector pMD.2G (both gifts from S. Karniely, Weizmann Institute, Israel) using transfection reagent FuGene6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... In vitro transcribed RNA derived from plasmid “pCI/SARS-CoV envelope” was synthesized using T7 RiboMAX Express Large Scale RNA production system (Promega), then purified by phenol/chloroform extractions and two successive precipitations with isopropanol and ethanol ...
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bioRxiv - Immunology 2020Quote: ... 293T cells were co-transfected with envelope-expressing plasmid and an env-deleted HIV-1 backbone plasmid (pSG3ΔEnv) using a FuGENE6 transfection kit (Promega Inc.). Cell supernatants containing pseudotyped viruses were harvested 48 h post transfection and used for infection in TZM-bl cells using DEAE-dextran (25 μg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μg of psPAX2 packing plasmid and 2 μg of pM2G envelope plasmid were transfected into HEK293 Lenti-X cells using 30 μL of Fugene-HD (Promega) in antibiotic-free DMEM ...
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bioRxiv - Cell Biology 2023Quote: Luciferase screening of Envelope subgenomic siRNA potentency and specificity was performed using the Dual-Luciferase Reporter Assay System (Promega, E19190). For initial screening of siRNA potency ...
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bioRxiv - Molecular Biology 2019Quote: ... and cDNA was generated using the Moloney Leukemia Virus Reverse Transcriptase (Promega), both according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2019Quote: ... and reverse transcribed using Moloney murine leukaemia virus reverse transcriptase enzyme (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... virus stocks were first treated with 50 units/ml RQ1-DNase (Promega) for 1 h to remove any plasmid DNA ...
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bioRxiv - Molecular Biology 2019Quote: ... and reverse transcribed with Moloney murine leukemia virus reverse transcriptase RNase H(−) (Promega) using oligoT primer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Each RNA sample was reverse transcribed using murine leukemia virus reverse transcriptase (Promega) and random DNA hexamer primers ...
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bioRxiv - Microbiology 2020Quote: ... Cells infected with Luciferase contaning virus were analyzed by Bright-Glo™ (Promega) using the manufacturers protocol ...
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bioRxiv - Immunology 2019Quote: ... cDNA was generated using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega) with random hexamer primers ...
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bioRxiv - Molecular Biology 2021Quote: ... and Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Promega, Madison, WI), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and Moloney murine leukemia virus M-MLV reverse transcriptase (Promega, Madison, WI, USA) was performed according to the vendor’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 8×106 HEK293T/17 cells were transfected with a proviral backbone plasmid and an envelope expression vector by using FuGENE HD (Promega, Madison, WI) and following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 10×106 HEK293T/17 cells were transfected with a proviral backbone plasmid and an envelope expression vector by using FuGENE6 (Promega, Madison, WI) and following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Pseudoviruses capable of single-cycle infection were prepared by transfecting 8×106 HEK293T/17 cells with a proviral backbone plasmid and an envelope expression vector by using FuGENE HD (Promega, Madison, WI) and following the manufacturer’s instructions as previously described46 ...
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bioRxiv - Neuroscience 2024Quote: ... or shRNA constructs with viral enzymes and envelope proteins (pMDLg/RRE, pRSV-REV and pVSV-G) using FuGENE6 transfection reagent (Promega Cat# E2692). HEK293T media was replaced with Neurobasal media (Thermo Fisher Scientific Cat# 21103049 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μg of pPAX packaging vector and 1 μg of VSV-G envelope vector using FuGENE® HD Transfection Reagent (Promega, cat.no. E2311), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Virus infectivity and neutralization was measured using a β-galactosidase-based luciferase assay (Promega) with TZM-bl target cells ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNAs were synthesised using Moloney Murine Leukemia Virus Reverse Transcriptase (Promega, www.promega.com). The quantification of gene expression was made by qRT-PCR ...
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bioRxiv - Genetics 2020Quote: ... Total RNA (0.5 µg) was reverse transcribed with mouse myeloblastosis virus reverse transcriptase (Promega) under standard conditions using hexanucleotide random primers according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... and then used for reverse transcription with avian myeloblastosis virus (AMV) reverse transcriptase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The virus-induced CPE was assessed using the Viral ToxGlo™ assay (Promega #G8943) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) system for RT-PCR (Promega) was used for cDNA synthesis using 500 ng extracted RNA from all samples ...
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bioRxiv - Molecular Biology 2024Quote: ... An equal amount of extension mix consisting of avian myeloblastosis virus reverse transcriptase (Promega), 5x AMV buffer and 40 μM dNTPs was added ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized with Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI). qRT-PCR was performed with the BioRad CFX96 Real-Time PCR System or Applied Biosystems 7000 ...