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Citations for Promega :
1 - 50 of 8248 citations for Dengue Virus Serotype 1 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... dengue protein NS2B/3/4A constructs were expressed in rabbit reticulocyte lysate (TNT coupled T7, Promega Bio Systems) programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag ...
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bioRxiv - Microbiology 2023Quote: ... 1×106 Sf9 insect cells were transfected with bacmid DNA using Fugene (Promega) and cultivated for three days ...
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bioRxiv - Synthetic Biology 2022Quote: ... insect Spodoptera frugiperda Sf21 cell line extract (Promega), and rabbit reticulocyte extract (Promega) ...
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bioRxiv - Developmental Biology 2021Quote: ... miPEPs sequences cloned into pF25A ICE T7 Flexi vector were expressed in vitro using TnT® T7 Insect Cell Extract Protein Expression System (Promega). For cells extracts and Drosophila extracts ...
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bioRxiv - Biophysics 2022Quote: ... Insect cells were transfected using Fugene HD transfection reagent (Promega, E2311). The virus was amplified in progressively larger cultures ...
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bioRxiv - Biophysics 2020Quote: ... coli and subsequently transfected into Sf9 insect cells using FuGENE (Promega) reagent ...
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bioRxiv - Microbiology 2022Quote: Cell viability of different 2D cultures at different time points of infection or exposure to virus proteins or HAE supernatant was determined using the CellTiter 96 AQueous One Solution cell proliferation assay (G3582; Promega), while HTO viability was determined by the CellTiter-Glo 3D cell viability assay (Promega G9681 ...
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bioRxiv - Microbiology 2021Quote: ... the cells were lysed with a 1:1 ratio of virus to Bright-Glo luciferase assay media (Promega, #E2610) and the contents were analyzed on a luminometer (LUMISTAR Omega ...
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bioRxiv - Genetics 2024Quote: ... Virus was concentrated using PEG virus precipitation (Promega # V3011). In brief ...
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bioRxiv - Cell Biology 2022Quote: ... Bacmids were transfected to Sf9 insect cells by lipofection (FuGENE 6 Transfection Reagent, Promega), to generate baculovirus ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... an envelope plasmid encoding VSV-G and pAdVAntage (Promega). Four days after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... obtained by amplification in DH10BacY cells were used to transfect Sf9 insect cells using FuGene transfection reagent (Promega). About 7 days after transfection the V0 virus was harvested and used to produce a V1 virus stock ...
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bioRxiv - Neuroscience 2024Quote: ... or shRNA constructs with viral enzymes and envelope proteins (pMDLg/RRE, pRSV-REV and pVSV-G) using FuGENE6 transfection reagent (Promega Cat# E2692). HEK293T media was replaced with Neurobasal media (Thermo Fisher Scientific Cat# 21103049 ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 unit/μl Avian Myoblastosis virus reverse transcriptase (Promega®) were added and incubated for 1h at 30°C ...
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bioRxiv - Biochemistry 2022Quote: ... Sf21 insect cells were transfected with PLP/DM20 bacmid using Fugene 6 transfection reagent (Promega Corp.) and baculoviruses were collected and used for preparation of a high-titer virus stock ...
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bioRxiv - Biochemistry 2023Quote: ... 2-4μg bacmid was transfected into Sf9 cells in 6 well plates using Insect-XPRESS (Promega E2311). Cells were incubated at 2°C for 5 days and bacmid YFP expression assessed by Leica DM IL LED Fluo microscope using a green fluorescent protein filter cube ...
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bioRxiv - Cancer Biology 2021Quote: ... psPAX2 (packaging plasmid) and pMD2.G (envelope plasmid) using FuGENE (Promega) or Lipofectamine3000 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and 0.25 μg VSV-G envelope plasmid using Fugene 6 (Promega) transfection reagent ...
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bioRxiv - Bioengineering 2023Quote: ... and envelope plasmid using calcium phosphate-mediated transfection method (Promega, E1200) and harvesting the supernatant 48 - 72 h after transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... WT and m6A-mut expressing cells in 24-well plates were infected with the indicated virus (MOI 1) and dual luciferase assay (Promega) was performed at 48 hours post-infection according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Cells infected with Luciferase contaning virus were analyzed by Bright-Glo™ (Promega) using the manufacturers protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μg of pPAX packaging vector and 1 μg of VSV-G envelope vector using FuGENE® HD Transfection Reagent (Promega, cat.no. E2311), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μg of psPAX2 packing plasmid and 2 μg of pM2G envelope plasmid were transfected into HEK293 Lenti-X cells using 30 μL of Fugene-HD (Promega) in antibiotic-free DMEM ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.2 μg of pMD2.G envelope plasmid using FuGENE 6 (Promega). Cells were incubated up to 48 hrs and then lentivirus-containing media was collected and concentrated with Lenti-X concentrator (Clontech) ...
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bioRxiv - Molecular Biology 2023Quote: ... the psPax2 envelope and the vsv-G packaging plasmids using Fugene6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 293T cells were co-transfected with envelope-expressing plasmid and an env-deleted HIV-1 backbone plasmid (pSG3ΔEnv) using a FuGENE6 transfection kit (Promega Inc.). Cell supernatants containing pseudotyped viruses were harvested 48 h post transfection and used for infection in TZM-bl cells using DEAE-dextran (25 μg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... Bacmid DNA was prepared by isopropanol precipitation and transfected into Sf9 (Spodoptera frugiperda) insect cells using FuGENE HD transfection reagent (Promega). After two rounds of virus amplification in Sf9 cells ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant viral DNA was extracted under alkaline conditions, followed by ethanol precipitation (Birnboim and Doly, 1979) and used for transfection into insect cells (Fugene HD, Promega). Baculovirus recombinant for COL6A3WT or COL6A3ΔC4 were propagated in Sf21 cells and supernatant (SN ...
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bioRxiv - Microbiology 2022Quote: In vitro translation assays of the wild type or mutant CrPV-1A RNAs were performed in Spodoptera frugiperda 21 (sf-21) insect cell extract (Promega). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 8×106 HEK293T/17 cells were transfected with a proviral backbone plasmid and an envelope expression vector by using FuGENE HD (Promega, Madison, WI) and following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 10×106 HEK293T/17 cells were transfected with a proviral backbone plasmid and an envelope expression vector by using FuGENE6 (Promega, Madison, WI) and following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Pseudoviruses capable of single-cycle infection were prepared by transfecting 8×106 HEK293T/17 cells with a proviral backbone plasmid and an envelope expression vector by using FuGENE HD (Promega, Madison, WI) and following the manufacturer’s instructions as previously described46 ...
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bioRxiv - Microbiology 2022Quote: In vitro cytotoxicity of SMKIs on mock and/or virus infected A549 cells was determined using CellTiter-Glo 2.0 (CTG) Cell Viability Assay (Promega) according to manufacturer protocols.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The viability of virus- and mock-infected cells were measured using Cell Titer Glow assay (CTG; Promega, Madison, USA). The luminescence was read with a PHERAstar FS plate reader (BMG Labtech ...
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bioRxiv - Microbiology 2022Quote: ... Virus was incubated with cells for 24 hours and cells were subsequently lysed with Renilla Luciferase Assay System (Promega) according to manufacturer instructions ...
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bioRxiv - Systems Biology 2019Quote: Synthesis of proteins was performed separately using the in vitro coupled transcription-translation system based on Insect Cell Extract (ICE) (TnT-T7-ICE, Promega, #L1102). In this ICE coupled transcription-translation system ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay Virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system (RNA1 qPCR primers GW194 and GW195)23.
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bioRxiv - Microbiology 2021Quote: All virus stocks were produced by plasmid transfection of HEK 293T cells with Fugene 6 (Promega). Supernatants were harvested at 48h and 72h ...
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bioRxiv - Microbiology 2020Quote: ... Cells transduced with luciferase expressing virus were assessed using Promega Steady-Glo system (Promega Madison, WI)
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bioRxiv - Immunology 2023Quote: ... Pseudotyped virus were prepared by transfection of HEK293T cells using the Fugene HD transfection system (Promega), as previously described(42) ...
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bioRxiv - Biochemistry 2021Quote: ... Reverse transcription was performed with 1 unit of Avian Myoblastosis Virus (AMV) reverse transcriptase (Promega®) at 42°C for 2 min ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: ... proteins from HL-1 cells were extracted with Passive Lysis Buffer (Promega France). Quantification of bioluminescence was performed with a luminometer (Centro LB960 ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins from HL-1 cells were extracted with Passive Lysis Buffer (Promega France). Bioluminescence was quantified with a luminometer (Centro LB960 ...
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bioRxiv - Microbiology 2019Quote: ... the cell viability of each infected virus variants was measured using CellTiter 96 Aqueous One solution Cell Proliferation Assay (Promega, USA). The relative cell viability was calculated by normalizing the absorbance value of treated virus samples against untreated virus samples ...
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bioRxiv - Biophysics 2020Quote: ... Virus infectivity was determined by luciferase production (Promega) after 48h using a Synergy2 Multi-Detection Microplate Reader (BioTek)
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bioRxiv - Microbiology 2020Quote: ... virus replication was measured by nLUC assay (Promega) using a SpectraMax plate reader (Molecular Devices).
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bioRxiv - Immunology 2020Quote: ... using Moloney murine leukemia virus reverse transcriptase (Promega) according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2020Quote: 1 µg of total RNAs were reverse transcribed with Moloney murine leukemia virus Reverse Transcriptase (M170B, Promega). 100 ng cDNA and FastStart Universal SYBR Green Master (04913850001 ...
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bioRxiv - Microbiology 2022Quote: ... virus was removed and cells were transfected with 2 µg of plasmid DNA pUCSP-A24Rcd using FuGeneHD (Promega) following manufacturer’s recommendations ...