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Citations for Promega :
1 - 50 of 696 citations for Cytomegalovirus Purified Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Purified EDIII antigens were biotinylated using HaloTag PEG biotin ligand (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2019Quote: ... Both constructs were carried by the same CytoMegaloVirus (CMV)-driven mammalian expression vector (pCI-neo derived, Promega) and were maintained at high copy number via the presence of the SV40 replication origin.
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bioRxiv - Cancer Biology 2022Quote: ... Biotinylated antigens were immobilized using streptavidin-coated magnetic beads (Promega). In each round ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... HaloTag-GST was used as a negative control antigen (Promega).
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bioRxiv - Cell Biology 2022Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer with Azure Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer with Azure Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... The following primary antibodies against the antigens were used: Tuj-1 (1:300, Promega), Synaptophysin (1:400 ...
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bioRxiv - Plant Biology 2020Quote: ... Antigen on the membrane was visualized with horseradish peroxidase-coupled anti-rabbit IgG (Promega) for VHA-a1 and VHA-a3 ...
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bioRxiv - Immunology 2021Quote: ... RBD and NTD antigens were site-specifically biotinylated using Halotag PEG biotin ligand (Promega), following the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... Antigen retrieval was performed by incubation with 20 μg/mL proteinase K (Promega, USA) for 20 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... selections were performed using biotinylated Fc fusion antigen captured on SA-coated magnetic beads (Promega). Prior to each selection ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 25% confluent wells were transfected with 2 µg plasmid vector expressing SV40 antigen using Fugene Transfection Reagent (Promega) as described by manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... incubating and chromogenic reaction generating were similar to the ACE2 assay instead of that primary antibody binding to antigen was not required and that HRP-conjugated goat anti-human IgG antibody (Promega) would be used as the secondary antibody to detect binding of CB6 antibody to the antigens.
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bioRxiv - Bioengineering 2020Quote: Phage selections were done according to previously established protocols.30 Selections were performed using biotinylated antigens captured with streptavidin-coated magnetic beads (Promega). In each round ...
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bioRxiv - Molecular Biology 2020Quote: ... NOCT antibodies were then purified from the verified serum by ammonium sulfate fractionation and then antigen affinity purification using recombinantly expressed HaloTag-NOCT (1-431) immobilized on HaloLink resin (Promega). The serum was purified over the immobilized NOCT column before elution with 5 M NaI and 1 mM ammonium thiocyanate ...
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bioRxiv - Cancer Biology 2019Quote: CHO K-1 cells overexpressing hPD-L1 and the recombinant TCR ligand (hPD-L1 Antigen Presenting Cells, hPD-L1 aAPCs, Promega) and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE ...
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bioRxiv - Genetics 2019Quote: ... Purified recombinant luciferase protein (Promega) was used to generate a standard curve.
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bioRxiv - Bioengineering 2021Quote: ... PCR product was purified (Promega) and digested using DpnI (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... with purified LgBiT Protein (Promega). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in a 6-well plate and transfected with 10 µg of linearised plasmid expressing the SV40 Large T antigen using the FuGene Transfection Kit (Promega, #E2691) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... PCR product was column purified (Promega) for subsequent in vitro transcription of nsp4 RNA using mMESSAGE mMACHINE T7 Transcription Kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... PCR product was column purified (Promega) for subsequent in vitro transcription of N RNA using mMESSAGE mMACHINE T7 Transcription Kit (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... or purified LgBiT-substrate cocktail (Promega). After incubation with appropriate HRP-tagged secondary antibodies and chemiluminescent substrate (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... or purified LgBiT-substrate cocktail (Promega), the blots were imaged and processed with a FluorChem E (Protein Simple).
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bioRxiv - Synthetic Biology 2024Quote: Purified NanoLuc protein (Promega REF G9711) was diluted to 10 µM in 0.1 mg/ml BSA solution ...
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bioRxiv - Molecular Biology 2022Quote: ... SEA (0.2 mg/ml) and soluble schistosomula antigens (0.2 mg/ml) was determined using the Caspase-Glo® 3/7 Assay System (Promega, Sydney, Australia) according to the manufacturers’ instructions.
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bioRxiv - Microbiology 2024Quote: ... the nLuc activity of each antigen was adjusted to comprise 107 RLU per second (RLU/s) in a volume of 50 µL (NanoGlow, Promega, Madison, Wisconsin, USA).
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bioRxiv - Molecular Biology 2019Quote: ... The natural templates include purified human (Promega), rhesus monkey (BioChain) ...
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bioRxiv - Neuroscience 2019Quote: ... and DNaseI-purified (Promega, Madison, WI, USA). cDNA was generated using the Superscript II protocol (Life Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... The PCR DNAs were gel purified (Promega Wizard SV Gel and PCR Clean-up System #A9282 ...
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bioRxiv - Genetics 2023Quote: ... Purified RNA was treated with DNase (Promega) at 37°C for 30 min ...
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bioRxiv - Genomics 2023Quote: ... purified Homo sapiens DNA (Promega, Madison, WI) with a microbial community standard (Zymo Research ...
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bioRxiv - Cell Biology 2020Quote: ... and purified using RNeasy MinElute Cleanup Kit (Promega) following standard protocols ...
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bioRxiv - Immunology 2021Quote: ... 400 µL PCR products were purified by Promega Wizard Gel/PCR purification columns ...
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bioRxiv - Neuroscience 2021Quote: ... purified with Wizard Genomic DNA Purification kit (Promega) and confirmed via sequencing (GeneWiz) ...
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bioRxiv - Bioengineering 2021Quote: ... and purified using the PureYield MidiPrep system (Promega).
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bioRxiv - Biophysics 2022Quote: ... and subsequently purified by gel purification kit (Promega).
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bioRxiv - Biophysics 2022Quote: ... the two purified flanking PCR fragments (Promega, USA) were assembled with BamHI and HindIII-digested PK18 (Gmr ...
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bioRxiv - Cell Biology 2020Quote: ... and purified using RNeasy MinElute Cleanup Kit (Promega) following the standard protocols ...
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bioRxiv - Biochemistry 2023Quote: ... purified PsVs were treated with DNase I (PROMEGA) to remove unencapsidated DNA associated with capsids ...
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bioRxiv - Microbiology 2022Quote: 0.5mg of plasmid DNA (purified pAdvantage plasmid, Promega), 1mg of bacterial genomic DNA (isolated from E ...
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bioRxiv - Neuroscience 2023Quote: ... Purified HaloTag protein (#G4491) was purchased from Promega. Pluronic F-127 (# P3000MP) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The purified RNA was treated with DNase I (Promega) to remove genomic DNA contaminants and then converted to cDNA using Applied Biosystems High Fidelity RT kit (ThermoFisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified DNA samples were quantified by Quantus (Promega Inc.) Fluorometer™ ...
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bioRxiv - Neuroscience 2021Quote: ... purified with phenol:chloroform:isoamyl alcohol (PCI) and Wizard resin (Promega), digested with XbaI and BamHI-HF in Cutsmart Buffer (NEB) ...
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bioRxiv - Genetics 2020Quote: ... Plasmids were purified with Wizard Plus SV Minipreps (Promega) and sequenced.
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bioRxiv - Genetics 2019Quote: ... purified and cloned into pGEM-T easy vector (Promega). Recombinant clones were then sent to Macrogen (South Korea ...
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bioRxiv - Genomics 2021Quote: ... and purified using SV Total RNA Isolation System (Promega). RNA samples were quantified ...