Labshake search
Citations for Promega :
1 - 50 of 4863 citations for Cow Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... elegans and mouse cDNA (PolyATtract® mRNA Isolation Systems, Promega and ProtoScript® First Strand cDNA Synthesis Kit ...
-
bioRxiv - Zoology 2020Quote: ... or pGL3-enhancer plasmid (negative control; Promega). The firefly luciferase readings were normalized by the Renilla luciferase reading to calculate the relative fold-change.
-
bioRxiv - Biochemistry 2022Quote: ... 0.01 % enhancer (ProteaseMAX™, V2071, Promega, USA) is predispensed into the wells ...
-
bioRxiv - Biochemistry 2024Quote: ... and 0.01% enhancer (ProteaseMAX, V2071, Promega, USA) and 1 % DMSO) ...
-
bioRxiv - Developmental Biology 2022Quote: ... All enhancer or mutant enhancer reporter constructs (200ng) were transfected with 20ng of pRL (Renilla luciferase control vector, Promega) and either empty vector (pcDNA3.1 ...
-
bioRxiv - Genetics 2020Quote: ... cloned into a pGL4.23 enhancer assay vector (Promega), and sequence-verified ...
-
bioRxiv - Genomics 2021Quote: ... Variant or variant haplotype fragments were amplified in African American heterozygous individuals carrying the variants of interest located in the middle of the fragments (Supplementary Table 2 and 3) and cloned into the enhancer reporter vector pGL4.24 (E8421, Promega). Subsequently ...
-
bioRxiv - Genomics 2020Quote: ... DNA was subcloned into the enhancer barrier vector28 (gift from Dr. Laura Elnitski, NHGRI) or pGL4.23 for enhancer assays (Promega, Madison, WI). Constructs were prepared using the Plasmid MidiPrep Kit (Qiagen ...
-
bioRxiv - Molecular Biology 2021Quote: ... one containing the SV40 promoter and enhancer (pGL3 Control, Promega, described as pSV40-luc in this manuscript ...
-
bioRxiv - Molecular Biology 2022Quote: ... one containing the SV40 promoter and enhancer (pGL3 Control, Promega, described as pSV40-luc in this manuscript ...
-
bioRxiv - Microbiology 2020Quote: ... 0.2% v/v ProteaseMax ™ surfactant trypsin enhancer (Promega, Madison, WI), 5mM dithiothreitol ...
-
bioRxiv - Microbiology 2020Quote: ... The standard was created with above-mentioned primers by amplifying mcrA genes from cow rumen fluid [19] and cloning them into the pGEM®-Teasy vector system (Promega, Mannheim, Germany). Amplicons for mcrA standard were generated with vector-specific primers sp6 and T7 and the resulting PCR product was cleaned with DNA purification kit (Biozym ...
-
bioRxiv - Microbiology 2024Quote: ... for 2 h at 25 °C and digested with trypsin (Promega, 37 °C, overnight) in TEAB buffer (50 mM ...
-
bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The enhancer constructs were transfected 24 h after seeding using FuGENE6 (Promega, US) as the transfection reagent ...
-
bioRxiv - Neuroscience 2022Quote: ... Purified and digested DNA fragment was cloned into a pGL3-Enhancer vector (Promega) and the correct sequence was checked by Sanger sequencing ...
-
bioRxiv - Biochemistry 2022Quote: ... 2) chymotrypsin and lysyl endopeptidase (lys-C) (Fujifilm Wako) 3) lys-C and trypsin (Promega) and 4 ...
-
bioRxiv - Microbiology 2019Quote: ... Proteins were digested for 2 h at 37°C with Lys-C and 16 h at 37°C with Trypsin Gold (both Promega). For TMT 6-plex labeling (Thermo Fisher Scientific) ...
-
bioRxiv - Systems Biology 2023Quote: ... and digested overnight at 37°C with a 2 μg Trypsin/Lys-C enzyme mix (Promega). The next day ...
-
bioRxiv - Genomics 2022Quote: ... containing a minimal CMV-promoter for enhancer-assays or pGL4.15[luc2P/Hygro] (Promega, E6701) w/o any promoter for promoter-assays were first digested using EcoRV (New England Biolabs ...
-
bioRxiv - Biochemistry 2020Quote: ... CellTiter-Glo 2 kit (Promega) using manufacturer’s instructions.
-
bioRxiv - Neuroscience 2021Quote: ... 2 μl of Trypsin/Lys-C mix (0.5μg/μl, Promega, V5073) was added to each sample and incubated for 3 hrs at RT in the dark ...
-
bioRxiv - Cell Biology 2022Quote: ... 2 µg Lys-C/Trypsin mix protease (Promega, Mass spec grade) were added to each sample (10 mM EPPS pH 8.5 ...
-
bioRxiv - Microbiology 2021Quote: ... 2 μg RNA was incubated at 30 °C for 2 hr in Spodoptera frugiperda (Sf21) extract (Promega) in the presence of [35S]methionine-cysteine (Perkin-Elmer ...
-
bioRxiv - Genomics 2023Quote: Reporter assays were conducted in HCT116 cells using the secreted NanoLuc enhancer activity reporter pNL3.3 (Promega) and normalized against a constitutively active firefly luciferase reporter vector ...
-
bioRxiv - Neuroscience 2022Quote: ... to a final urea concentration of 2 M for overnight Trypsin/Lys-C digestion at 35°C (1:100 protease:substrate ratio, Mass Spectrometry grade, Promega Corporation ...
-
bioRxiv - Bioengineering 2023Quote: ... and digested for 2 hours at 50°C using Trypsin Platinum (Promega) using 1:50 Trypsin to sample ratio by mass ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein was then digested with 2.5 μg Lys-C (Wako, Japan) for 4 h at 37°C and 2 μg trypsin (Promega, V5113) for an additional 4 h at 37°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... The pellet was resuspended in 100-μL of 0.2% Protease Max Surfactant Trypsin Enhancer (Promega Cat# V2071), 50-mM ABC ...
-
bioRxiv - Cancer Biology 2021Quote: Each enhancer or promoter region was amplified from human genomic DNA and cloned into pGL4.10 [luc2] (Promega) containing a SNP (rs718960 ...
-
bioRxiv - Cell Biology 2023Quote: ... the 200k pellets were digested for 2 h at 37°C with 0.4 µg of Trypsin/Lys-C (Promega CAT#: V5071) and then overnight by adding 0.4 µg of Trypsin/Lys-C ...
-
bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by 2 h Trypsin/Lys-C mix incubation (V5073, Promega) and collected ...
-
bioRxiv - Cell Biology 2019Quote: ... the proteins were digested with 2 μg of trypsin/Lys-C mix (Promega) for 16 h at 37°C ...
-
bioRxiv - Biochemistry 2023Quote: ... 10 µL of 2 ng/µL trypsin/Lys-C Mix (CAT# V5072, Promega) in 0.3% acetic acid was added to a 96-well plate (CAT# 0030128664 ...
-
bioRxiv - Molecular Biology 2021Quote: ... FASP was carried out in two 10 kDa MWCO filters with a 50 mM iodoacetamide alkylation step and proteins were digested in 2M urea with 2% wt/wt Lys-C (Wako) for 6 h and 2% modified trypsin (Promega) for 12 h at 37°C ...
-
bioRxiv - Developmental Biology 2022Quote: CEBPa promoter (−2kb) or enhancer region (Cooper et al., 2015) was cloned into the pGL3 basic backbone (Promega). The construct containing part of the Id1 promoter region (336 bp ...
-
bioRxiv - Molecular Biology 2019Quote: Candidate enhancer sequences were synthesized as dsDNA (IDT) and cloned into the pGL4.24 destabilized Luciferase reporter vector (Promega) using the HiFi DNA Assembly method (NEB) ...
-
bioRxiv - Genomics 2022Quote: ... HepG2 cells were seeded in 24-well plates at a density of 100,000 cells/well and transfected with 400 ng pGL4.23-enhancer vector + 40 ng pRL-TK renilla vector (Promega) with Lipofectamine 3000 reagent ...
-
bioRxiv - Cell Biology 2020Quote: ... Columns were washed with 90% methanol/100 mM TEAB 10 times prior to on-column digestion with 1 ug Trypsin/Lys-C in 50 mM TEAB (47° C, 2 hours; Cat. #V5073, Promega, Madison, WI). The resulting peptides were eluted with sequential washes of 50 mM TEAB ...
-
bioRxiv - Molecular Biology 2023Quote: ... Japan) at 37 C for 2 h before digestion overnight with 1 ug Trypsin/Lys-C mix (V5071, Promega, Madison, WI, USA) at 37 C ...
-
bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
-
bioRxiv - Biochemistry 2020Quote: ... Digestion solutions were brought to a final concentration of 2 M urea and 2 mM CaCl2 and a second digestion was performed overnight at 37 °C using trypsin (Promega, v5280) at 1:100 w/w ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 h at 37 °C with 2 μg trypsin (Promega). After digestion ...
-
bioRxiv - Molecular Biology 2020Quote: Prg4 enhancer/promoter-firefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector (Promega). Bovine genomic DNA was extracted from bovine chondrocytes and was used as the template to amplify Prg4 regulatory regions ...
-
bioRxiv - Molecular Biology 2020Quote: Prg4 enhancer/promoter-firefly luciferase reporters were co-transfected together with the pGL4.75 [hRlu/CMV] renilla luciferase reporter (Promega) into either superficial zone chondrocytes ...
-
bioRxiv - Neuroscience 2021Quote: Luciferase reporter constructs were constructed by cloning a candidate enhancer region into the pGL3 promoter vector (Promega; Madison, WI). Each region was inserted using standard restriction enzyme-based cloning techniques ...
-
bioRxiv - Genomics 2020Quote: ... Each well was transfected with 25ng of the corresponding putative enhancer-cloned pGL3-promoter luciferase reporter along with 1.5ng Renilla (pRL-TK Vector, Promega) control reporter vector using FuGENE HD transfection reagent as previously described [20] ...
-
bioRxiv - Genomics 2022Quote: MM001 and MM047 were seeded in 24-well plates and transfected with 400 ng pGL4.23-enhancer vector + 40 ng pRL-TK renilla vector (Promega) with Lipofectamine 2000 (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2022Quote: Luciferase reporter constructs were generated by cloning a candidate enhancer region into the pGL3 promoter vector (Promega; Madison, WI). Each region was inserted using standard restriction enzyme-based cloning techniques ...
-
bioRxiv - Genomics 2023Quote: ... Reference allele enhancer sequences for hZRS and hs737 were obtained via PCR cloning from human genomic DNA (Promega, G304A). Primers used for each enhancer sequence are outlined in Table S2 ...