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Citations for Promega :
1 - 50 of 5143 citations for Cow Four And A Half LIM Domains Protein 2 FHL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Half the lysate was used to quantify luciferase protein (Promega Luciferase Assay System #E1501) using a Spectramax L luminometer with reagent injectors in technical triplicate ...
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bioRxiv - Cell Biology 2022Quote: ... MYLK4 (MYLK4-NLuc) and TYK2(JH2 domain) (TYK2(JH2 domain)-NLuc) were kindly provided by Promega. NLuc (nanoluciferase ...
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bioRxiv - Cancer Biology 2019Quote: FAK1 kinase domain (AA393-698) (Promega) (25 μg ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were trypsinized and half re-plated and half harvested for a luciferase assay system (Promega). This luciferase activity was considered as the baseline activity ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were trypsinized and half re-plated and half harvested for a luciferase assay system (Promega). This luciferase activity was the baseline activity ...
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bioRxiv - Cell Biology 2022Quote: ... Trapped proteins were washed four times with methanol TEAB buffer followed by overnight Trypsin (1 μg, Promega) digestion at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... PGC-1α full-length and protein domain were translated in vitro in the T7 transcription and translation coupled reticulocyte system (Promega) supplemented with 35S-Methionine ...
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bioRxiv - Microbiology 2022Quote: ... the FLAG-APEX2 was fused in-frame upstream of the GBF1 truncated after HDS1 domain and containing a BFA-resistant Sec7 domain from ARNO (GARG-1060) in a mammalian expression vector pCI (Promega) generating a plasmid pCI-FLAG-APEX2-GARG- 1060 ...
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A DNA-based optical nanosensor for in vivo imaging of acetylcholine in the peripheral nervous systembioRxiv - Bioengineering 2020Quote: ... four pHAb fluorophores (Amine-reactive, Promega) and two BTX molecules (AF 647 conjugated ...
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bioRxiv - Microbiology 2020Quote: ... The standard was created with above-mentioned primers by amplifying mcrA genes from cow rumen fluid [19] and cloning them into the pGEM®-Teasy vector system (Promega, Mannheim, Germany). Amplicons for mcrA standard were generated with vector-specific primers sp6 and T7 and the resulting PCR product was cleaned with DNA purification kit (Biozym ...
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bioRxiv - Cancer Biology 2021Quote: All kinase assays for AURKA catalytic domain were performed with ADP-Glo™ Kinase Assay Kit (Promega©). For Alisertib the concentrations tested were ...
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bioRxiv - Cell Biology 2022Quote: ... The trapped proteins were washed four times with the methanol TEAB buffer and then digested using 1 µg Trypsin (Promega) overnight at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
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bioRxiv - Genetics 2020Quote: Yeast genomic DNA was prepared from 1 ml cultures grown in rich media from single colonies or Ade− half-sectors using the Wizard® Genomic DNA Purification Kit (Promega). Genomic DNA (150 ng ...
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bioRxiv - Molecular Biology 2021Quote: ... then diluted four times before overnight trypsin digestion (Promega) at a 1:100 ratio ...
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bioRxiv - Zoology 2022Quote: Four μl 100-bp DNA ladder (Promega, Southampton, UK) and 40 μl of the PCR products were loaded with 5 μl Promega 6× DNA loading dye on 1.5% agarose gels (peqGOLD universal agarose ...
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bioRxiv - Cancer Biology 2020Quote: ... Forty-eight hours post transfection (or twenty-four hours for promoter truncation/mutation) dual-luciferase reporter assay kit (Promega) was used to analyze bioluminescence on SpectraMax i3x microplate reader (Molecular Devices ...
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bioRxiv - Biochemistry 2020Quote: ... CellTiter-Glo 2 kit (Promega) using manufacturer’s instructions.
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bioRxiv - Biophysics 2023Quote: ... while half-DNA-CPC 1 was labelled with JF-549-Halo (Promega) in the same way ...
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bioRxiv - Microbiology 2020Quote: ... four serial 100-fold dilutions of pGem-T Easy (Promega) plasmids ...
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bioRxiv - Genomics 2021Quote: ... Tris(2-carboxyethyl)phosphine (TCEP) and BCA protein assay kit were from Pierce and sequence-grade trypsin was from Promega. Rapigest and Sep-Pak C18 columns were from Waters and C18 Zip tips were from Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... the protein suspension was digested with 2 µg trypsin (Promega) overnight at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... the protein suspension was digested with 2 µg trypsin (Promega) overnight at 37°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Luciferase activity was assayed in an Omega luminescence plate-reader (Fluostar) with four different gains following the instructions of the Nano Dual-Luciferase® Reporter kit (Promega). A further correction for luminescence bleed-through was applied using the software developed by Mauri et al ...
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bioRxiv - Systems Biology 2019Quote: ... Half of the cDNA library was PCR amplified using Pfu polymerase (Promega, M7745) using the P5 forward PCR oligonucleotide and barcoded BC reverse oligonucleotides (200 nM ...
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bioRxiv - Cell Biology 2020Quote: ... based on a mutated RIIB cAMP-binding domain from PKA (pGloSensor-20F, Promega), which produces rapid and reversible cAMP-dependent activation of luciferase activity in intact cells and is capable of detecting cAMP elevations in the absence of phosphodiesterase inhibitors ...
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bioRxiv - Genetics 2021Quote: ... and Pierce 660nm Protein Assay kit (Promega) was used for quantification ...
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bioRxiv - Immunology 2022Quote: ... Half the cells were then recovered and used for Cell Titer Glo assay (Promega). Half the cells were stained with Annexin V Apoptosis Detection Kit (Biolegend ...
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bioRxiv - Microbiology 2024Quote: Recombinant FIKK kinase domains activity was measured using the ADP-Glo kinase assay (Promega), which quantifies the amount of ADP produced during the kinase reaction ...
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bioRxiv - Molecular Biology 2020Quote: ... The bacterial surface proteins with attached plasma proteins were released by limited proteolysis with 2 μG trypsin (Promega) /37 °C ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... AccuMAP™ Low pH Protein Digestion Kit (Promega) according to the manufactures protocol ...
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bioRxiv - Systems Biology 2019Quote: ... Half of the cDNA (5 μl) was amplified by PCR using Pfu Polymerase (Promega, M7745) with the cycling conditions (95°C for 2 min ...
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bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
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bioRxiv - Systems Biology 2023Quote: ... Half of the lysis was treated with 5 µl of 10 mg/ml Rnase (Promega:527491) and both lystates (+/- RNase ...
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bioRxiv - Cell Biology 2023Quote: ... Top half was incubated in anti-Nup107 (1:1000; ab236634) and HaloTag (1:500; Promega G9211). Bottom half was incubated in anti-GAPDH (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Half of the sample was digested with 10 µg trypsin (Promega, 18 h at 37 °C). DOC was precipitated with 10 µL concentrated formic acid (conc ...
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bioRxiv - Cancer Biology 2023Quote: ... viable cells were quantified in a FLUOstar OPTIMA ELISA reader (550/590 nm) about 2 h after CellTiter-Blue staining (Promega, Cat No. G8081). Mean values +/-standard deviations (SD ...
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bioRxiv - Molecular Biology 2020Quote: ... The nanoluciferease sequence fused to a PEST domain was amplified from pNL1.2[NlucP] Vector Sequence (Promega) and was cloned into a modified CD813A vector (System Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... The nanoluciferease sequence fused to a PEST domain was amplified from pNL1.2[NlucP] Vector Sequence (Promega) and was cloned into a modified CD813A vector (System Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: Proteins bound to beads were reduced with 2 mM DTT in 2 M urea buffer and sequencing grade trypsin (Promega) was added to a final concentration of 5 ng/μl ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by 2 h Trypsin/Lys-C mix incubation (V5073, Promega) and collected ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were digested with 2 μg of trypsin/Lys-C mix (Promega) for 16 h at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were digested by 2 µg sequencing grade trypsin (Promega, Madison, WI) at 37°C with shaking overnight ...
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bioRxiv - Cell Biology 2020Quote: ... The protein concentration was estimated using a BCA kit (Promega). The blots were blocked using 5% NFDM (Santa Cruz ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using Bradford Assay Kit (Promega, USA) and equal amounts of proteins were denatured ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 h at 37 °C with 2 μg trypsin (Promega). After digestion ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were further digested by the addition of 2 μg trypsin (Promega #V5113) for 4 hours at room temperature on a shaker ...
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bioRxiv - Cell Biology 2022Quote: ... Baseline fluorescence was measured in an ELISA reader (Promega) following an incubation of 30 min at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... the plate was equilibrated at room temperature during half an hour and CellTiter-Glo® Reagent (Promega, G7570) was added according to manufacturer instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Half the cells were used for assaying luciferase activity using the Dual-Luciferase Reporter Assay System (Promega, E1980) to measure Firefly (Fluc ...