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Citations for Promega :
1 - 50 of 92 citations for Cone Arrestin Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... β-arrestin 2 fused at the N-terminus to LgBiT (LgBiT-β-arrestin 2; Promega, plasmid no. CS1603B118) was chosen as it has previously been used successfully with other class B GPCRs ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 0.05 μg LgBiT-β-arrestin-2 (Promega) plus 0.9 µg pcDNA3.1 for 24 hours ...
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bioRxiv - Cell Biology 2021Quote: HEK293 T-REx SNAPf-GLP-1R-SmBiT cells were seeded in 12-well plates and co-transfected with 0.1 μg β-arrestin-2 fused at the N-terminus to LgBiT (LgBiT-β-arrestin-2; Promega, plasmid no. CS1603B118) and either 0.1 μg mini-Gs-Venus or mVenus supplemented with 0.8 μg pcDNA 3.1.
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bioRxiv - Neuroscience 2021Quote: ... Cells used for growth cone analyses were stained for βIII-tubulin (1:1000, Promega), Phalloidin 568 conjugate (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plasmid for β-arrestin-2 fused at the N-terminus to LgBiT was obtained from Promega custom assay services (plasmid CS1603B118) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or with 0.05 µg GLP-1R-SmBiT and 0.05 µg LgBit-β-arrestin-2 (Promega, Hampshire, UK) plus 0.9 µg pcDNA3.1 for 24 hours ...
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bioRxiv - Biochemistry 2023Quote: The recruitment of PTH1R to β-arrestin was detected in HEK293 cells using the NanoLuc Binary System (NanoBiT; Promega). The Lgbit subunit was fused to the C-terminus of PTH1R and the SmBiT subunit was fused to the N-terminus of β-arrestin ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The plasmid for β-arrestin-2 fused at the N-terminus to LgBiT was obtained from Promega (plasmid no. CS1603B118). Construction of the GLP-1R-SmBiT plasmid was described previously (55 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plasmid for β-arrestin-2 fused at the N-terminus to LgBiT was obtained from Promega (plasmid no. CS1603B118); this configuration was used due to previous success with another class B GPCR (Shintani et al. ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293T cells were transiently transfected using Lipofectamine 2000 with 50 ng each of GLP-1R-SmBit and LgBit-β-arrestin-2 (Promega) diluted in pcDNA3.1 ...
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bioRxiv - Molecular Biology 2021Quote: ... SmBiT34 (peptide 86, Promega) subunit was added to the C terminus of Rat Gβ1 with a 15AA polypeptide linker between them ...
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bioRxiv - Biophysics 2021Quote: ... C1 peptide was from Promega (Madison, WI). Lyophilized ATP was from Expedeon (Cambridge ...
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bioRxiv - Systems Biology 2020Quote: ... and an external reference peptide mix (Promega) was spiked into the sample at the concentration of 0.5 pmol/µl ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were digested using trypsin/LysC (Promega) overnight with head-over-head mixing ...
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bioRxiv - Biochemistry 2024Quote: ... a K562 human peptide digest standard (Promega) and E.coli peptide standard (Waters ...
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bioRxiv - Molecular Biology 2020Quote: ... HRP Conjugate (Promega, 1:10000 in Blocking one), was added to the membrane ...
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bioRxiv - Cell Biology 2020Quote: ... alkylated and digested into peptides with trypsin (Promega). The resulting peptides were desalted and concentrated before mass spectrometry by the STAGE-TIP method using a C18 resin disk (3M Empore) ...
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bioRxiv - Zoology 2020Quote: After digestion into short peptides by trypsin (Promega), the samples were analyzed using a DiNa nanoLC system (KYA Technologies ...
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bioRxiv - Biochemistry 2022Quote: LC-MS/MS Peptide Reference Mix (V7491, Promega) in HeLa digest (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were eluted by on-bead trypsin (Promega) digest and acidified for MS analysis ...
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bioRxiv - Immunology 2022Quote: ... alkylated and digested into peptides using trypsin (Promega). Peptides were desalted and concentrated using Empore-C18 StageTips and eluted with 0.5% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... st-Ht31 inhibitor peptide was from Promega (Madison, WI).
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bioRxiv - Biophysics 2021Quote: ... 49 (peptide 86 and also named as HiBiT, Promega) with a 15-amino acid (15AA ...
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bioRxiv - Plant Biology 2023Quote: ... Peptides were digested using sequencing grade trypsin (V5113; Promega) diluted in 50 mM Triethylammonium bicarbonate buffer pH 8.5 (T7408 ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were digested using sequencing grade trypsin (V5113; Promega) diluted in 50 mM Triethylammonium bicarbonate buffer pH 8.5 (T7408 ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins/peptides were further digested with trypsin (Trypsin Gold, Promega) at 37 °C overnight ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were released by digestion with PNGase F (Promega, Madison, WI) overnight at 37 °C with vortexing ...
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bioRxiv - Systems Biology 2022Quote: ... peptides were separated were spotted on streptavidin-conjugated membranes (Promega #V2861) where they tightly associated via their C-terminal biotinylations ...
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bioRxiv - Biochemistry 2021Quote: ... A 15-amino acid linker and SmBiT subunit (peptide 86, Promega) were attached to the C-terminus of rat Gβ1 ...
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bioRxiv - Developmental Biology 2022Quote: ... Purified protein was then digested into peptides using Trypsin (Promega, V5111) and desalted using Pierce C18 Spin Columns (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... and then the proteins were digested to peptides using trypsin (Promega) at a trypsin/protein ratio of 1:50 (w/w ...
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bioRxiv - Biochemistry 2024Quote: ... and 10 units of Peptide N-glycosidase F (PNGase F, Promega) enzymes according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... PCR products for the peptide genes were subcloned into pGEM-T (Promega) and sequenced ...
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bioRxiv - Cell Biology 2020Quote: ... LAMP1-HaloTag transfected cells were incubated with the JF646 peptide (Promega, GA1121) at 200 nM for 15 min ...
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bioRxiv - Cell Biology 2023Quote: InCELLect™ AKAP St-Ht31 inhibitor peptide was from Promega (Chilworth, UK). Human fibrinogen was from Enzyme Research (Swansea ...
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bioRxiv - Immunology 2023Quote: ... Tissues were permeabilized and blocked in blocking buffer with 0.05% Tween 20 (H5152, Promega) and 5% BSA (A3059 ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were then digested to peptides with sequencing grade modified trypsin (Promega V5113), at 37 °C overnight.
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bioRxiv - Biochemistry 2023Quote: ... and peptides were generated by in-gel digestion using trypsin (sequencing-grade (Promega)) at 37°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... The peptides were spotted onto streptavidin-coated filter sheets (Promega SAM2 biotin capture membrane) and visualized by phosphorimaging on Typhoon FLA 7000 ...
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bioRxiv - Biochemistry 2020Quote: N-glycans were released from nMPO using Elizabethkingia miricola peptide-N-glycosidase F (Promega) (Jensen et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... Rat Gβ1 was cloned with a C-terminal SmBiT34 (peptide 86 or HiBiT, Promega) connected with a 15AA polypeptide linker ...
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bioRxiv - Bioengineering 2020Quote: ... Peptides were then produced by trypsin digestion (50 ng trypsin, Promega Sequence Grade, Modified) (Liu et al ...
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bioRxiv - Physiology 2020Quote: The protein solution samples were hydrolyzed into peptide segments by trypsin (V9012, Promega (Beijing) Biotech Co. ...
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bioRxiv - Biochemistry 2021Quote: The fused peptide–mRNA was subsequently reverse transcribed using MMLV RT RNase H- (Promega) for 1 h at 42 °C and 0.5 μL aliquot of the peptide-mRNA fusions was taken from the mixture and saved for the determination of the total amount of inputted mRNA ...
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bioRxiv - Physiology 2022Quote: ... Peptides contained C-terminal biotinylations and were spotted on streptavidin-conjugated membranes (Promega #V2861) and imaged with Typhoon FLA 7000 phosphorimager (GE ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were then digested into peptides with sequencing grade modified trypsin (Promega V5113) at 37 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... peptides were collected as described in ProteaseMax application note (Promega, TB373, Rev. 2/15), and purified using Pierce C18 Spin Tips (Thermo Fisher PI84850) ...
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bioRxiv - Biophysics 2024Quote: The fused peptide–mRNA was subsequently reverse transcribed using MMLV RT Rnase H-(Promega) for 1 h at 42 °C ...
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bioRxiv - Biophysics 2019Quote: ... Generation of tryptic peptides for mass spectrometry was performed with the aid of ProteaseMax (Promega) detergent ...
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bioRxiv - Biochemistry 2021Quote: ... chymotrypsin and peptide-N-glycosidase F (PNGase F) were obtained from Promega (Madison, WI, USA). Centrifugal filters (0.5 mL ...