Labshake search
Citations for Promega :
1 - 50 of 2589 citations for Collagen 17 alpha 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... knowlesi elongation factor-1 alpha (pkef1-alpha) 5’ UTR were isolated from agarose gels and cloned into the pGEM-T vector (Promega, Madison, WI, USA). To replace the pfcam 5’ UTR sequence that drives luciferase transcription in the pD-pfcam-Luc ...
-
bioRxiv - Molecular Biology 2020Quote: ... HRP Conjugate (Promega, 1:10000 in Blocking one), was added to the membrane ...
-
bioRxiv - Cancer Biology 2020Quote: ... 17 Synergy assessments were performed using CellTiter-Glo (Promega) as previously described26 with the following modifications ...
-
bioRxiv - Molecular Biology 2021Quote: ... SmBiT34 (peptide 86, Promega) subunit was added to the C terminus of Rat Gβ1 with a 15AA polypeptide linker between them ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... and thereafter digested with 17 ng/μl sequencing-grade trypsin (Promega) in 25 mM NH4HCO3 using a slightly modified in-gel digestion protocol (Shevchenko et al ...
-
bioRxiv - Molecular Biology 2019Quote: ... and finally digested with 17 ng/μl sequencing-grade trypsin (Promega) in 25 mM NH4HCO3 using a slightly modified in-gel digestion protocol (Shevchenko et al. ...
-
bioRxiv - Molecular Biology 2023Quote: ... and finally digested with 17 ng/μl sequencing-grade trypsin (Promega) in 25 mM ABC ...
-
bioRxiv - Bioengineering 2021Quote: ... and collagen content via a QuantiFlour dsDNA assay kit (Promega), a modified 1,9-dimethylmethylene blue (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2023Quote: ... The reaction was initiated by the addition of 1-250 nM acylated peptide (Vivitide) and a 1:10,000 dilution of LgBiT subunit of the NanoBiT luciferase (Promega) and read using an Envision plate reader (XCite 2105 ...
-
bioRxiv - Biochemistry 2023Quote: ... Bovine alpha-2-hs-glycoprotein was isolated from plasma (Fetuin, Promega, V4961), and monocyte differentiation antigen CD14 was recombinantly expressed in CHO cells (R&D systems ...
-
bioRxiv - Cell Biology 2020Quote: ... 293T/17 and HeLa cells were transfected with plasmids using FugeneHD (Promega) or Lipofectamine3000 (Thermo Fisher Scientific) ...
-
bioRxiv - Bioengineering 2022Quote: ... and collagen content were assessed via Quantifluor dsDNA assay kit (Promega), a modified 1,9-dimethylmethylene blue (DMMB ...
-
bioRxiv - Biophysics 2023Quote: ... Collagens were then digested using Sequencing Grade Modified Trypsin from Promega with a ratio of 1:50 (trypsin ...
-
bioRxiv - Immunology 2022Quote: ... Proteins were digested into peptides with 12μL of 1:10 Trypsin Gold (Promega, Madison, Wisconsin, V528A) in 50mM TEAB per sample ...
-
bioRxiv - Biophysics 2023Quote: ... the proteins were digested into tryptic peptides by incubation with 1□µg sequencing grade trypsin (Promega) overnight at 37□°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... The membrane was probed with α-Halotag mouse monoclonal antibody (Promega, diluted 1:1000 in Blocking Buffer), washed 3 times in TBS-Tween ...
-
bioRxiv - Molecular Biology 2020Quote: ... then probed with goat α-mouse HRP-conjugated secondary antibody (Promega, diluted 1:10000 in Blocking Buffer) and finally washed 3 times in TBS-Tween ...
-
bioRxiv - Biochemistry 2022Quote: ... the primary antibody was diluted in blocking buffer as follows: Anti-HaloTag (1:1000; Promega Corporation; G9281); anti-PHC1 antibody (1:500 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Triplicate biological replicates were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described68 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Quadruplicate biological replicates were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described 60 ...
-
bioRxiv - Immunology 2021Quote: ... After 17-20 h 50 μL/well of One-Glo-EX substrate (Promega) was added to the cells and incubated in the dark for 5-10 min prior reading on a Varioskan LUX plate reader (ThermoFisher) ...
-
bioRxiv - Biophysics 2021Quote: ... C1 peptide was from Promega (Madison, WI). Lyophilized ATP was from Expedeon (Cambridge ...
-
bioRxiv - Systems Biology 2020Quote: ... and an external reference peptide mix (Promega) was spiked into the sample at the concentration of 0.5 pmol/µl ...
-
bioRxiv - Cell Biology 2023Quote: ... Peptides were digested using trypsin/LysC (Promega) overnight with head-over-head mixing ...
-
bioRxiv - Biochemistry 2024Quote: ... a K562 human peptide digest standard (Promega) and E.coli peptide standard (Waters ...
-
bioRxiv - Cancer Biology 2020Quote: ... Pellets were reconstituted and digested with endoproteinase Lys-C (Alpha Laboratories, UK) for 1 hour at room temperature and trypsin (Promega, Madison, WI, USA) overnight at 35°C.
-
bioRxiv - Bioengineering 2023Quote: ... and collagen content were then determined via Quantifluor dsDNA assay kit (Promega), modified 1,9-dimethylmethylene blue (DMMB ...
-
bioRxiv - Cell Biology 2024Quote: ... biological replicates (n=7) were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described (Hubner et al. ...
-
bioRxiv - Immunology 2022Quote: ... After 17-20 hours VSV 40 μL/well of One-Glo-EX substrate (Promega) was added to the cells and incubated in the dark for 5-10 min prior reading on a BioTek plate reader ...
-
bioRxiv - Cancer Biology 2019Quote: ... ATP solution (5 μL of a 100 μM solution containing 0.1 μg 4:1 glycine:tyrosine peptide substrate (Promega)) was added and incubated at 37 °C for 20 min before the addition of ADP-Glo reagent (5μL ...
-
bioRxiv - Plant Biology 2023Quote: ... Total proteome peptide pools were generated by sample digestion overnight with 1:100 sequencing grade trypsin (V5113; Promega). Generated peptide pools were quantified by Nanodrop ...
-
bioRxiv - Cell Biology 2020Quote: ... alkylated and digested into peptides with trypsin (Promega). The resulting peptides were desalted and concentrated before mass spectrometry by the STAGE-TIP method using a C18 resin disk (3M Empore) ...
-
bioRxiv - Zoology 2020Quote: After digestion into short peptides by trypsin (Promega), the samples were analyzed using a DiNa nanoLC system (KYA Technologies ...
-
bioRxiv - Biochemistry 2022Quote: LC-MS/MS Peptide Reference Mix (V7491, Promega) in HeLa digest (Thermo Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... peptides were eluted by on-bead trypsin (Promega) digest and acidified for MS analysis ...
-
bioRxiv - Immunology 2022Quote: ... alkylated and digested into peptides using trypsin (Promega). Peptides were desalted and concentrated using Empore-C18 StageTips and eluted with 0.5% (v/v ...
-
bioRxiv - Neuroscience 2022Quote: ... The mixture was then incubated with 1 μg of AKT substrate II peptide (CKRPRAASFAE) as the Pak4 substrate (Promega) or 1 μg of GST-Raf13 as the Pak1 substrate with a total volume of 5 μL in a 384-well plate (PerkinElmer ...
-
bioRxiv - Cell Biology 2020Quote: ... st-Ht31 inhibitor peptide was from Promega (Madison, WI).
-
bioRxiv - Biophysics 2021Quote: ... 49 (peptide 86 and also named as HiBiT, Promega) with a 15-amino acid (15AA ...
-
bioRxiv - Plant Biology 2023Quote: ... Peptides were digested using sequencing grade trypsin (V5113; Promega) diluted in 50 mM Triethylammonium bicarbonate buffer pH 8.5 (T7408 ...
-
bioRxiv - Plant Biology 2024Quote: ... Peptides were digested using sequencing grade trypsin (V5113; Promega) diluted in 50 mM Triethylammonium bicarbonate buffer pH 8.5 (T7408 ...
-
bioRxiv - Immunology 2023Quote: ... Three biological replicates for each condition were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) “on beads” as previously described [44] ...
-
bioRxiv - Cancer Biology 2022Quote: ... luciferase activity was measured as previously described (17) using the Dual-Glo Luciferase assay system (Promega) using a Promega GloMax Explorer multimode plate reader.
-
bioRxiv - Biochemistry 2022Quote: ... Proteins/peptides were further digested with trypsin (Trypsin Gold, Promega) at 37 °C overnight ...
-
bioRxiv - Cancer Biology 2021Quote: ... Biological triplicates were digested with Lys-C (Alpha Laboratories, Eastleigh, UK) and trypsin (Promega, Madison, WI, USA) on beads as previously described (56) ...
-
bioRxiv - Zoology 2020Quote: ... rehydrated with 17 μl 25 mM ammonium hydrogen carbonate containing 4.5 ng/μl sequencing grade trypsin (Promega) and 0.025% Proteasemax (Promega ...
-
bioRxiv - Biochemistry 2020Quote: Human ClCN1 cDNA was expressed from pCDNA 3.1(+) expression vector in HEK293T/17 cells (ATCC) by transient transfection using Fugene 6.0 reagent (Promega) according to the manufacturer’s specifications ...
-
bioRxiv - Microbiology 2020Quote: ... Peptides were released by digestion with PNGase F (Promega, Madison, WI) overnight at 37 °C with vortexing ...
-
bioRxiv - Systems Biology 2022Quote: ... peptides were separated were spotted on streptavidin-conjugated membranes (Promega #V2861) where they tightly associated via their C-terminal biotinylations ...
-
bioRxiv - Biochemistry 2021Quote: ... A 15-amino acid linker and SmBiT subunit (peptide 86, Promega) were attached to the C-terminus of rat Gβ1 ...