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Citations for Promega :
1 - 50 of 927 citations for Coiled Coil Domain Containing 28B CCDC28B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the FLAG-APEX2 was fused in-frame upstream of the GBF1 truncated after HDS1 domain and containing a BFA-resistant Sec7 domain from ARNO (GARG-1060) in a mammalian expression vector pCI (Promega) generating a plasmid pCI-FLAG-APEX2-GARG- 1060 ...
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bioRxiv - Cell Biology 2022Quote: ... MYLK4 (MYLK4-NLuc) and TYK2(JH2 domain) (TYK2(JH2 domain)-NLuc) were kindly provided by Promega. NLuc (nanoluciferase ...
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bioRxiv - Cancer Biology 2019Quote: FAK1 kinase domain (AA393-698) (Promega) (25 μg ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were resolved in reducing and non-reducing SDS-PAGE gels and membranes were incubated overnight at 4°C with the following antibodies: rabbit polyclonal anti-human p75 intracellular domain (1:1000, Promega); mouse monoclonal anti-HA (1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ... based on a mutated RIIB cAMP-binding domain from PKA (pGloSensor-20F, Promega), which produces rapid and reversible cAMP-dependent activation of luciferase activity in intact cells and is capable of detecting cAMP elevations in the absence of phosphodiesterase inhibitors ...
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bioRxiv - Microbiology 2024Quote: Recombinant FIKK kinase domains activity was measured using the ADP-Glo kinase assay (Promega), which quantifies the amount of ADP produced during the kinase reaction ...
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bioRxiv - Molecular Biology 2020Quote: ... The nanoluciferease sequence fused to a PEST domain was amplified from pNL1.2[NlucP] Vector Sequence (Promega) and was cloned into a modified CD813A vector (System Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... The nanoluciferease sequence fused to a PEST domain was amplified from pNL1.2[NlucP] Vector Sequence (Promega) and was cloned into a modified CD813A vector (System Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: All kinase assays for AURKA catalytic domain were performed with ADP-Glo™ Kinase Assay Kit (Promega©). For Alisertib the concentrations tested were ...
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bioRxiv - Genomics 2021Quote: ... full-length BORIS and CTCF 11 ZF domain were translated in vitro using the TnT Quick Coupled Transcription/Translation System (Promega).
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bioRxiv - Cell Biology 2021Quote: ... while the sequences for NSD2 or NSD3 PWWP1 domain was cloned in-frame into a pNLF1-C [CMV/Hygro] vector from Promega using the In-Fusion HD Cloning kit (Takara) ...
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bioRxiv - Cell Biology 2020Quote: Identification of the β5-integrin carboxyl domain as Src substrate was determined using the ADP-Glo Kinase Assay (Promega, V6930) following protocols recommended by the manufacturer ...
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bioRxiv - Biochemistry 2021Quote: ... Halo-tagged ubiquitin-binding domains (UBDs) of TUBE (tandem-repeated ubiquitin-binding entities) was incubated with HaloLink resin (200 μL, Promega) in binding buffer (50 mm Tris⍰HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... PGC-1α full-length and protein domain were translated in vitro in the T7 transcription and translation coupled reticulocyte system (Promega) supplemented with 35S-Methionine ...
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bioRxiv - Physiology 2021Quote: ... A K13 donor template consisting of a 1.5 kb region of the K13 coding region including the entire propeller domain was amplified using the primer pair p3+p4 and cloned into the pGEM T-easy vector system (Promega). This donor sequence was subjected to site-directed mutagenesis in the pGEM vector to introduce silent binding-site mutations at the Cas9 cleavage site using the primer pair p5+p6 ...
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bioRxiv - Biophysics 2023Quote: ... insertion of the FUS N-terminal domain) were synthesized and sequenced by the Genewiz Company from a pFN22A vector (Promega) containing the insert HaloTag-p53 WT ...
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bioRxiv - Developmental Biology 2021Quote: A partial TWD1 fragment of 150 bp covering the TPR domain and a part of the calmodulin binding domains (aa 264-314) was PCR amplified and cloned into pGEM-T-Easy Vector (Promega Corp.) and verified by sequencing ...
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bioRxiv - Biophysics 2021Quote: ... and the twitchin kinase domain lacking flanking tails (Kin) was measured by monitoring ATP hydrolysis using the Kinase Glo™ luminescent assay system (Promega). Assays were performed using 10 µg of protein sample in a 50 µL reaction volume ...
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bioRxiv - Biophysics 2019Quote: ... MDA-MB-231 cells were stably transfected with the plasmids coding for 3-phosphoinositide-specific Akt-PH domain fused with CFP and the plasmids coding for Rac1 fused with YFP using FuGENE® HD transfection reagents (Promega). MCF-7 cells and MDA-MB-231 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... ligation of double digested fragment of either domain with double digested pAcGFP1-N1 was performed with the LigaFAst Rapid DNA Ligation System (Promega, M8226) following the manufacturer’s suggested protocol ...
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bioRxiv - Cell Biology 2020Quote: ... and the sequence encoding a genetically modified firefly luciferase into which a cAMP-binding domain has been inserted from the pGloSensor-20F (Promega, Cat #E1171).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... before re-suspending in 50 μl flow buffer containing anti-Nluc polyclonal primary antibody raised in rabbit (Kindly gifted by Promega) at 1:100 dilution and incubated at room temperature for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were blocked with PBS containing 5% skim milk and incubated with HRP-conjugated anti-human IgG1 antibodies (Promega, #W403B) overnight at 4 °C.
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 24-48hrs in the blocking solution containing chicken anti-human NT-4/5 antibody (10 mg/ ml, Promega, Madison, WI) or rabbit anti-human NT-4/5 (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... containing 100ng/μL trypsin (Promega) or 100ng/μL GluC (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... and dNTPs containing dUTP (Promega). A single 3’ “A” base was added to the end-repaired cDNA ...
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bioRxiv - Systems Biology 2023Quote: ... containing 0.5 µg Trypsin (Promega), 0.1 µg Lysyl Endopeptidase (FUJIFILM Wako ...
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bioRxiv - Developmental Biology 2023Quote: ... containing RNase inhibitor RNasinTM (Promega). FACS analysis and sorting were performed on a (BD FACS Aria III ...
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bioRxiv - Molecular Biology 2023Quote: ... containing 5mg/mL BSA(Promega) dissolved in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... containing 50mM DTT (Promega, #V3151), vortexed and heated up to 98°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... containing: Green 1X Go Taq (Promega) buffer ...
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bioRxiv - Neuroscience 2021Quote: ... were transfected into monolayer culture cells with pGL4.29 containing CRE-luciferase reporter gene or pGL4.33 containing SRE-luciferase reporter gene (9.0 μg/dish, Promega) and pGL4.74 containing the Renilla luciferase reporter gene (0.5 μg/dish ...
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bioRxiv - Microbiology 2022Quote: ... containing 13.94 µl RNase free water (Promega), 6.25 µl master mix (Eurogentec) ...
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bioRxiv - Microbiology 2020Quote: ... in water) containing Rapid Digestion Trypsin (Promega) using a protein/enzyme (w/w ...
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bioRxiv - Bioengineering 2020Quote: ... containing 0.8 U/μL RNasin Plus (Promega) and GAPDH or KRT19 primers (primer sequences listed in Table S1) ...
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bioRxiv - Microbiology 2021Quote: ... 1% SDS) containing protease inhibitor cocktail (Promega), Sodium fluoride (10 mM ...
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bioRxiv - Biochemistry 2022Quote: ... containing 500 ng trypsin/LysC (Promega V5073) and digested for 16 hours at 37 °C ...
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bioRxiv - Biochemistry 2019Quote: ... containing approximately 10 ng Trypsin Gold (Promega) and incubated overnight at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... containing 4 μg/ml RNase A (Promega) for 10 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... containing 1 mM ethylenediaminetetraacetic acid (EDTA, Promega). The culture was then induced with 2 mM ZnCl2 and a 1 mL-sample was collected at 5 ...
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bioRxiv - Genetics 2019Quote: ... pRL-TK containing Renilla luciferase (Promega, USA), was simultaneously transfected into the cells with expression plasmids as an internal control for assay-to-assay variability ...
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bioRxiv - Neuroscience 2020Quote: ... containing a cocktail of protease inhibitors (Promega). Following solubilization by 1% Triton X-100 ...
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bioRxiv - Microbiology 2023Quote: ... 1% SDS) containing protease inhibitor cocktail (Promega), Sodium fluoride (10 mM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... containing a 2× concentration of Endurazine (Promega) was added to each well ...
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bioRxiv - Genomics 2023Quote: ... containing 1mM D-Luciferin Potassium Salt (Promega).
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bioRxiv - Molecular Biology 2020Quote: ... 1mM EGTA) containing 1U/µL RNAsin Plus (Promega) and lysed adding 800 µl of cold lysis buffer (1% Triton X-100 ...
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bioRxiv - Cell Biology 2021Quote: ... containing 10 nM TMR-conjugated Halo-ligand (Promega) at 37°C for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... containing 1X GoTaq qPCR Master Mix (Promega Corporation), 300 nM CXR Reference Dye and final concentration 200nM of each SYBR green designed primers described in Supplementary Table 2 ...
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bioRxiv - Cell Biology 2021Quote: ... containing 1X GoTaq qPCR Master Mix (Promega Corporation), 300 nM CXR Reference Dye ...
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bioRxiv - Biochemistry 2022Quote: ... and rehydrated with a buffer containing trypsin (Promega). Samples were digested overnight at 37°C and acidified using ? ...