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Citations for Promega :
1 - 50 of 3692 citations for Breast Cancer Type 1 Susceptibility Protein BRCA1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2019Quote: The wild type and mutant 3’ UTR of BRCA1 were constructed into psi-check-2 vector (Promega, Madison, WI, USA). Cells were plated in 24-well plates and the plasmids (psi-check-2-WT ...
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bioRxiv - Microbiology 2021Quote: Drug susceptibility assays using CellTiter-Glo 2.0 (Promega) were performed as previously described [6 ...
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bioRxiv - Cancer Biology 2019Quote: ... Stable cell lines were generated by co-transfecting pOG44 with pCDNA5/FRT/TO vectors encoding BRCA1 tBRCT (aa 1620-1862) in a 9:1 ratio using FuGENE HD transfection reagent (E2311, Promega). After 10 days of selection using Blasticidin (15μg/ml ...
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bioRxiv - Biochemistry 2022Quote: Human cancer cell line digest standards K562 (Promega) and diluted to 100 microgram/mL in 50mM TEAB and labeled with TMTPro reagents according to manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Protein detection was done using primary antibody anti-HaloTag monoclonal antibody (1:1,000 dilution, Promega), primary antibody anti-MamE polyclonal antibody (1:3,000 dilution ...
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bioRxiv - Microbiology 2020Quote: The phenotypic drug susceptibility assays were performed as previously described using CellTiter-Glo v2.0 (CTG) (Promega, Madison, WI)44,49,63 ...
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bioRxiv - Biochemistry 2021Quote: Standard cancer digest K562 and HeLa were obtained from Promega and Pierce ...
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bioRxiv - Plant Biology 2022Quote: ... Protein expression was confirmed by Western blot with Anti-Halo Tag monoclonal antibody (1:2000) (G9211, Promega).
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were detected with horseradish peroxidase (HRP)-conjugated rabbit or mouse secondary antibody [1:1,000 dilution] (Promega). The chemiluminescence signal was imaged using an Odyssey XF Imager (LI-COR ...
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bioRxiv - Genetics 2022Quote: Type-1 quantitative bioluminescence resonance energy transfer (qBRET) experiments utilised the NanoBRET platform (Promega) and followed the general type-1 qBRET protocol reported by Felce et al.25 The assay was performed in HEK293 cells transiently expressing POPDC1 and POPDC2 constructs which possessed a C-terminal NanoLuc luciferase or HaloTag domains ...
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bioRxiv - Cancer Biology 2020Quote: ... Cancer Research UK Manchester Institute) and pCDH-EF1α-DGAT1-T2A-mApple viral vectors using Fugene (Promega) following standard protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... the viability of adherent cancer cells was measured by the CellTiter-Glo® Luminescent Assay (Promega, G9242).
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bioRxiv - Biochemistry 2021Quote: ... the sample was diluted 1:1 with 50 mM ammonium bicarbonate and proteins were digested with trypsin (protein to enzyme ratio 1:50, Promega, Mannheim, Germany) overnight at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... 11S protein was detected with a rabbit polyclonal antibody from Promega, and Tom40 with a rabbit polyclonal antibody produced by Cambridge Research Biochemicals (Billingham ...
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bioRxiv - Immunology 2024Quote: Recombinant antibodies were purified using protein G magnetic beads (Promega #G7472), HiTrap protein G HP columns (Cytiva #17040401) ...
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bioRxiv - Biophysics 2020Quote: CosM6 cells were transfected with ∼1 µg of wild type or mutant constructs using FuGENE6 (Promega) and were patched within 1-2 days after transfection ...
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bioRxiv - Cancer Biology 2021Quote: The transcriptional activation of cMyc in liver cancer cells was measured using a Dual-Luciferase Assay Kit (Promega) on a GloMax 20/20 luminometer (Promega ...
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bioRxiv - Cancer Biology 2021Quote: ... All cancer cell lines were authenticated by short tandem repeats profiling using PowerPlex 1.2 System (Promega, Cat# DC8942), and periodically tested for mycoplasma infection using a commercial kit (Lonza ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were digested with 1 µg trypsin (Promega) and subjected to liquid chromatography (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and the viability of the cancer cells was assessed by measuring luminescence with a GloMax Discover plate reader (Promega) or an IVIS Lumina III XR (Perkin-Elmer ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were obtained in carrier/ protein-free buffer or were purified using the Magne Protein A or G Beads (Promega) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... In-solution digestion was performed with LysC-Trypsin mix (1:100 enzyme: protein ratio) and trypsin (Promega; 1:50 enzyme: protein ratio). Peptides were desalted on C18 stage tips ...
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bioRxiv - Plant Biology 2021Quote: ... Protein production was confirmed by western blot using an anti-HaloTag antibody (Promega). HaloTag-ligand conjugated magnetic beads (Promega ...
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bioRxiv - Physiology 2019Quote: ... Proteins were co-precipitated with 1 µg trypsin (Promega) overnight at −20 °C in 1 ml methanol ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were digested with 1) chymotrypsin (Promega, Madison, USA), 2 ...
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bioRxiv - Neuroscience 2023Quote: ... then with trypsin (Promega, 1:50 (protease to protein)) for 6 hours on a 37 °C shaker.
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bioRxiv - Genetics 2024Quote: ... A 1:10 ratio (enzyme: protein) of Trypsin (Promega) and LysC (Wako ...
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bioRxiv - Cell Biology 2024Quote: ... containing trypsin (Promega; final 1/100 enzyme/protein ratio) and LysC (Wako ...
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bioRxiv - Molecular Biology 2023Quote: ... and digested with Lys-C (1/200 weight of protein; Fujifilm Wako) and sequencing grade modified trypsin (1/100 weight of protein; Promega, Madison, WI, USA) at 37 °C overnight in 0.1 % Rapigest ...
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bioRxiv - Biophysics 2020Quote: ... antibodies followed by a 1-hour incubation with the corresponding HRP-conjugated secondary antibodies (1:2000, Promega) at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used are anti-HaloTag monoclonal antibody (Promega, G921A, 1:1000), and polyclonal rabbit antibodies ...
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bioRxiv - Immunology 2022Quote: ... and digested over-night with Lys-C-Trypsin mix (1:100 enzyme to protein ratio) and trypsin (Promega; 1:50 enzyme to protein ratio). Following the digestion step ...
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bioRxiv - Cell Biology 2022Quote: ... other cell types were transfected using Fugene HD (Promega) or Lipofectamine 2000 (Life technologies ...
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bioRxiv - Immunology 2022Quote: ... Proteins were digested with 20:1 protein:trypsin (Trypsin Gold, Promega) in 50 mM ammonium bicarbonate for 3 hours at 47 °C before adding an additional 1 µg of trypsin and digesting for a further 1 hour at 47 °C ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then digested with 1:50 trypsin-protein (Promega) at 37°C overnight in the dark ...
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bioRxiv - Immunology 2022Quote: ... trypsin (protein to enzyme ratio 1:50, Promega, Mannheim, Germany) was added for overnight digestion at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested with endoproteinase-LysC (Promega, 1:100, w:w) for 4 h at 37 °C and then with trypsin (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were digested with trypsin (Promega; 1:100 (w/w) enzyme-to-protein ratio ...
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bioRxiv - Immunology 2023Quote: ... Proteins were cleaved overnight with 1 µg of trypsin (Promega) at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies used: ßgal (1:100, Promega), pMad (1:800 ...
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bioRxiv - Microbiology 2022Quote: ... Both cassettes were built by first cloning the wild-type locus and 1 kb flanking region in the pGEM-T plasmid (Promega) and subsequently modifying the plasmid to add the HA-tag coding sequence using the Q5 Site-Directed Mutagenesis kit (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... The partial frame of circ_0005962 containing the binding site or mutant binding site (wild-type or mutant-type) with miR-382-5p was amplified and cloned into the downstream of pGL4 vector (Promega, Madison, WI, USA) to generate circ_0005962-wt and circ_0005962-mut fusion plasmids ...
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bioRxiv - Molecular Biology 2019Quote: ... for 30 minutes at room temperature and then digested by LysC (Lysyl endopeptidase, #125-05061, Wako, ratio LysC/protein 1:100) and trypsin (Trypsin Gold, V5280, Promega, ratio trypsin/protein 1:20) overnight ...
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bioRxiv - Cell Biology 2022Quote: ... (ii) wild-type cells transfected with mEGFP using Fugene6 (Promega), (iii ...
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bioRxiv - Immunology 2019Quote: ... Proteins were digested with sequencing grade trypsin (1:100; Promega, USA) overnight at 37°C and peptides concentration was checked by spectrophotometry (Nanodrop 2000 ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were on-bead digested with 1 µg trypsin (Promega) for 4 hours at 37 °C under agitation ...
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bioRxiv - Plant Biology 2021Quote: ... and tryptic protein digestion (1:100 enzyme:protein, V5111, Promega, Madison, USA) was performed using Microcon-30 kDa filter units (Millipore ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were digested overnight with trypsin (1:50) (Promega, USA) in 1% (w/v) ...
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bioRxiv - Biochemistry 2023Quote: ... then digested with lysC/trypsin mixture (1: 50, enzyme: protein, Promega) at 37℃ overnight ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested by addition of 1 µg of Trypsin (Promega) and incubated overnight at 37 °C at 600 rpm on a thermoshaker ...