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Citations for Promega :
1 - 50 of 2629 citations for Borrelia burgdorferi C t Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... at 72° C for 10 min to add 3’-A overhangs for T/A cloning with pGEM-T Easy plasmid (Promega). A 610 bp DNA fragment upstream of flgV was amplified using PCR primers 85 and 86 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Proteins were digested with Lys-C/Trypsin (Promega) at 37 °C overnight with rotation ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were digested first by Lys- C (Promega, USA) at RT for 2 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were digested first by Lys-C (Promega, USA) at RT for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested using trypsin/ Lys-C mixture (Promega) and desalted on a 10 x 4.0 mm C18 cartridge (Restek ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested overnight at 37 °C with Trypsin/Lys-C Mix (Promega, Charbonnières, France) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested overnight at 37 °C with trypsin (Promega) with an enzyme ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested by Trypsin/Lys-C Mix (Promega). After the digestion ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight at 25L°C with trypsin (Promega) with an enzyme/substrate ratio of 1:250 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at 37°C with trypsin (Promega) and Lys-C (Wako,125-05061 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were then incubated with Lys-C/trypsin mixture (Promega) (200 ng for pull-down samples and 1 ug for total lysate samples ...
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bioRxiv - Microbiology 2022Quote: ... the modified region was amplified using primer SAM_Seq_Gen-23_FW (5’-GAT TTG AGG ACG ACT GGA CTG C) and SAM_Seq_Gen1116_RV (5’-GTC AAC TGA ACA ACC CCA AGG T) together with GoTaq polymerase (Promega), followed by cloning into pGEM T-easy vector system (Promega ...
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bioRxiv - Microbiology 2023Quote: ... the modified region was amplified using primer SAM_Seq_Gen-23_FW (5’-GAT TTG AGG ACG ACT GGA CTG C) and SAM_Seq_Gen1116_RV (5’-GTC AAC TGA ACA ACC CCA AGG T) together with GoTaq polymerase (Promega), followed by cloning into pGEM T-easy vector system (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... at 72°C for 20min and then TA cloned into the pGEM-T Easy vector (Promega, Madison, WI). The cloned PCR products were Sanger Sequenced with M13 Forward (5’-TGTAAAACGACGGCCAGT-3’ ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were digested with Trypsin/ Lys-C (Promega, WI, USA). According to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested overnight at 37°C with 1µg trypsin (Promega). Peptides were desalted on C18 StageTips ...
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bioRxiv - Neuroscience 2019Quote: ... Resins were transferred to a new tube and proteins were digested with 0.1 µg Lys-C for 2h at 37°C and 0.5 µg trypsin (Promega) overnight at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... In-gel digestion of proteins was performed at 37 °C overnight using 2.5 μg Trypsin/Lys-C (Promega). Samples were further digested with 2.5 μg Chymotrypsin (Promega ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were digested overnight at 37°C with 800 ng trypsin (Promega). Digestion was stopped by adding 5 μl TFA (Trifluoroacetic acid ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested with 1 μg trypsin/Lys-C mix (Promega) at 37 °C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were digested at 37°C for 18 hr with trypsin (Promega) at a concentration of 1:25 (w/w ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were digested over night at 37 °C with trypsin (V5111, Promega) at a 1:20 enzyme to protein mass ratio ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight with Sequencing Grade Modified Trypsin/Lys-C (Promega) at 370C ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested with 4 ug Trypsin/Lys-C mix (Promega V5073) for 3-4 hrs at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were cleaved via trypsin and Lys-C endoproteinase (Promega, Madison, WI), at a ratio of 1:50 enzyme to protein overnight (12-16 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at 37°C with 0.2 μg trypsin (Promega). Formic acid was then added to 2% and the samples were incubated at 37°C for 30 min to cleave RapiGest ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were digested using trypsin/Lys-C mixture (Mass Spec Grade, Promega) for 16 h according to the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2019Quote: ... or into the pGEM-T or pGEM-T Easy vector (Promega) and subsequently sequenced (Macrogen).
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bioRxiv - Developmental Biology 2023Quote: ... The modified proteins were digested with lysyl endopeptidase (Lys-C) (Wako) for 3h at 37° C and subsequently digested with trypsin (Promega) overnight at 37° C ...
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bioRxiv - Genomics 2020Quote: ... A double-stranded DNA adapter with a T-overhang was ligated for 1 h at 20°C and the resulting products were bead purified (ProNex, Promega), eluted and then split into two identical aliquots ...
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bioRxiv - Molecular Biology 2021Quote: ... The sequences ligated by the T4 ligase at 4 °C for 12 h were cloned in the pGEM T-easy vector (Promega). The competent Escherichia coli X10-Gold (Stratagene ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by 2 h Trypsin/Lys-C mix incubation (V5073, Promega) and collected ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were digested with 2 μg of trypsin/Lys-C mix (Promega) for 16 h at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... proteins were digested overnight at 37°C by the addition of trypsin (Promega) at a 1:100 enzyme:substrate ratio ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested with 0.7 μg Trypsin/Lys-C Mix (MS grade, Promega) in 50 mM ammonium bicarbonate overnight at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... destained and protein digested overnight at 37°C with trypsin (Promega, Madison, Wisconsin). The digested peptide samples were desalted and concentrated using C18 zip tips (Eppendorf ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were digested at room temperature with 0.5 μg Lys-C (Promega, # VA1170) for 4 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Proteins were digested with Lys-C (Wako Chemicals) and trypsin (Trypsin Gold, Promega).
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bioRxiv - Biochemistry 2021Quote: ... Proteins were subjected to digestion using 20 µg sequencing grade Glu-C (Promega) in 1 M urea at 37°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... the proteins were digested with 1.5 µg of Trypsin/Lys-C mix (Promega) for 16 h at 37°C (800 rpm) ...
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bioRxiv - Microbiology 2023Quote: ... proteins were digested using a trypsin and lys-C mix (Promega, Madison, USA) at a ratio of 100:1 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were then digested overnight at 37°C with Trypsin Gold (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were digested with Lys-C (Wako) and sequencing-grade trypsin (Promega, V5117) (1 μg of each in 20 μL of 50 mM TEAB ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were digested with 1 µg of trypsin/Lys-C mix (Promega) for 16 h at 37 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... or pGEM-T (Promega) vectors and eight positive colonies/gene transferred to 750 ul of ddH2O and heated to 90 C to liberate the plasmids ...
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bioRxiv - Microbiology 2019Quote: ... proteins were firstly digested for 5 h at 37°C with 250 ng rLys-C Mass Spec Grade (Promega, Madison, USA). Samples were then diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration of 2M urea and then re-incubated overnight at 37°C with 1 µg Sequencing Grade Modified Trypsin (Promega ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were first digested for 5 h at 37 °C with 500 ng rLys-C Mass Spec Grade (Promega, Madison, WI, USA) before being diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration below 2 M urea ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were precipitated with isopropanol50 and digested overnight at 37°C with 1:50 enzyme : protein ratio by Trypsin/Lys-C Mix (Promega GmbH, Germany). Resulting peptides were desalted on MicroSpin column (The Nest Group ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were digested with Lys-C (Wako Chemicals) overnight and trypsin (Trypsin Gold, Promega) for 6 hours.
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bioRxiv - Neuroscience 2020Quote: ... The proteins were digested overnight at 37°C with Sequencing Grade Modified Trypsin (Promega) and the reaction was stopped by acidification ...