Labshake search
Citations for Promega :
1 - 34 of 34 citations for Bordetella Pertussis Toxin Frozen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... cytotoxicity of Bordetella infection was measured as lactate dehydrogenase (LDH) release into cell culture media using CytoTox 96 assay (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... Toxin sensitivity was measured with CellTiter-Glo reagent (Promega) according to manufacturer protocol ...
-
bioRxiv - Plant Biology 2020Quote: ... Frozen root tissue was extracted in Reporter Lysis Buffer (Promega) and LUC activity was measured with the Luciferase Assay Reagent (Promega ...
-
bioRxiv - Plant Biology 2022Quote: ... beads were flash frozen in liquid nitrogen and trypsinized via standard protocols (Promega). Peptides were labeled with 8-plex iTRAQ66 ...
-
bioRxiv - Plant Biology 2020Quote: ... flash frozen and RNA was extracted using the ReliaPrep™ RNA Tissue Miniprep System (Promega) according to the manufacturer’s specifications ...
-
bioRxiv - Systems Biology 2019Quote: ... Enzymatic protein hydrolysis was initiated by the addition of trypsin (Sequencing Grade Modified Trypsin, Frozen, Promega) at a ratio of 1:50 ...
-
bioRxiv - Genomics 2021Quote: ... from freshly frozen brain tissues or the Wizard genomic DNA purification kit (Promega, Madison, WI, USA) from blood according to manufacturer instructions ...
-
bioRxiv - Plant Biology 2021Quote: ... tissue was snap frozen and RNA isolated with the Maxwell® RSC Plant RNA kit (Promega). 1 µg of RNA was reverse transcribed into cDNA with the High-Capacity cDNA Reverse Transcription Kit with RNase inhibitor (Applied BiosystemsTM) ...
-
bioRxiv - Cell Biology 2019Quote: HepG2 cells or frozen mouse liver were sonicated in PBS containing protease and phosphatase inhibitors (Promega) in an ice-water bath using a Branson 250 digital sonifier programmed to cycle at a 10% amplitude with 5 seconds on followed by 20 seconds off ...
-
bioRxiv - Molecular Biology 2020Quote: ... Total RNA was isolated from frozen cell pellets using a Maxwell RSC SimplyRNA Cells kit (Promega) using a 2x concentration of DNAse I ...
-
bioRxiv - Systems Biology 2019Quote: ... and enzymatic protein hydrolysis was initiated by the addition of trypsin (Sequencing Grade Modified Trypsin, Frozen, Promega) at a ratio of 1:50 ...
-
bioRxiv - Biochemistry 2020Quote: ... Total RNA was extracted from frozen pools with 10 eggs/larvae with Maxwell HT simplyRNA (Promega corporation) according to the manufacturer’s instructions using Biomek 4000 Automated liquid handler (Beckman coulter) ...
-
bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted from the frozen samples using the SV Total RNA Isolation kit (Promega, Beijing, China), and cDNA was synthesized using the PrimeScript RT reagent Kit with gDNA Eraser (Takara ...
-
bioRxiv - Immunology 2022Quote: DNA was extracted from snap frozen intestinal tissue samples with the Wizard® Genomic DNA Purification Kit (Promega) per manufacturer’s instructions for animal tissue.
-
bioRxiv - Plant Biology 2021Quote: ... Samples were frozen in liquid nitrogen and total RNA extracted using the SV Total RNA Isolation System (Promega). RNA concentrations were determined using a NanoDrop 8000 (Thermo Scientific) ...
-
bioRxiv - Synthetic Biology 2021Quote: Protoplasts were flash frozen in liquid nitrogen and lysed by adding 30 μl of Passive Lysis Buffer (Promega). NanoLuc activity was then measured using the Nano‐Glo® Luciferase Assay (Promega ...
-
bioRxiv - Microbiology 2022Quote: Total RNA was extracted from liquid nitrogen quick-frozen tissue using a Total RNA Extraction Kit (LS1040; Promega) as per the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2022Quote: RNA was harvested from snap frozen cell pellets using the Maxwell®ū RSC simplyRNA Cells kit (Promega), and the accompanying Maxwell®□ RSC 48 instrument ...
-
bioRxiv - Cancer Biology 2023Quote: ... RNA extraction was performed on the frozen cores using the Maxwell 16 LEV simplyRNA Tissue Kit (Promega, AS1280). Specimens were prepared for RNA-seq as described above and RNA quality was verified using Agilent Bioanalyzer 2100 (Agilent Technologies) ...
-
bioRxiv - Systems Biology 2020Quote: Frozen fly brains were resuspended in 100 μL of lysis solution containing 0.1% (w/v) ProteaseMAX Surfactant (Promega, USA), 50 mM ammonium bicarbonate ...
-
bioRxiv - Plant Biology 2019Quote: ... frozen and ground in liquid nitrogen and resuspended in 1x passive lysis buffer (Dual Luciferase Assay kit, E1910, Promega), using 2 volumes/mg of material ...
-
bioRxiv - Microbiology 2019Quote: ... genomic DNA was extracted from frozen samples by using the Wizard Genomics DNA purification kit (Promega, Madison, WI, USA). One microliter of purified DNA was analysed by gel electrophoresis to check for integrity ...
-
bioRxiv - Microbiology 2020Quote: gDNA was extracted from stationary phase evolved cocultures following revival from frozen stocks using a Wizard Genomic DNA purification Kit (Promega). DNA fragment libraries were constructed for samples from shaking WT-based cocultures A-F and NifA*-based cocultures M-R at generation ∼146 using NextFlex Bioo Rapid DNA kit ...
-
bioRxiv - Microbiology 2019Quote: ... Plates were frozen for at least 30 minutes at −80°C before thawing and reading on a GloMax Multi plate reader (Promega). Briefly ...
-
bioRxiv - Biochemistry 2022Quote: ... The 90% buffer was removed and protoplasts were flash-frozen in liquid nitrogen to quench the protein degradation process followed by luciferase activity assay (E1500, Promega) to determine the relative amount of RAP2.12(1-28)-luciferase chimera protein remaining at various time points ...
-
bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from frozen ground tissues using the innuPREP Plant RNA kit (Analytic Jena) and digested with RQ1 DNase (Promega). First-strand cDNA synthesis was conducted on 1 µg RNA using the SuperScript III Reverse Transcriptase (Invitrogen ...
-
bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from FACS purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively.
-
bioRxiv - Microbiology 2024Quote: ... cells were infected at MOI 1 for 24 h and cells were frozen dry at -80°C for 30 min and lysed in Passive Lysis Buffer (Promega). Bioluminescence was measured using the Nano-Glo® Luciferase Assay System (Promega ...
-
bioRxiv - Genetics 2021Quote: Total RNA was extracted from cultured cells or frozen adipose tissue using the Eastep Super Total RNA Extraction Kit (Promega (Beijing) Biotech Co. ...
-
bioRxiv - Physiology 2022Quote: ... DNA was isolated from snap-frozen ACA tissues with the Maxwell® 16 Tissue DNA Purification Kit (#AS1030, Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... culture supernatants were frozen at −s80°C and macrophages were processed according to the Promega E1500 Luciferase Assay System protocol (Promega, Madison, WI). Cells were lysed and incubated with D-Luciferin substrate for 5 min in white Lumitrac™ plates (Greiner Bio-one ...
-
bioRxiv - Plant Biology 2024Quote: ... RNA was isolated from frozen harvested materials using the SV Total RNA isolation kit with on-column DNA digestion (Promega, Madison, WI, USA). The purity of all RNA samples was confirmed using a NanoDrop2000 instrument (Thermo Scientific) ...
-
bioRxiv - Cancer Biology 2019Quote: ... DNA was extracted from the tumor tissue and blood samples previously frozen using Maxwell® RSC Tissue DNA Kit (cat# AS1610, Promega, Madison, WI, USA) and Maxwell® RSC Blood DNA Kit (cat# AS1400 ...
-
bioRxiv - Genomics 2019Quote: mRNA was isolated from frozen samples using the Maxwell® 16 AS2000 instrument with a Maxwell® 16 LEV simplyRNA Tissue Kit (both Promega Corporation, Madison, WI, USA). The mRNA was isolated according to protocol with a modified lysis step as described in Snoek et al ...