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Citations for Promega :
1 - 50 of 2545 citations for Astrovirus Antigen Type 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... The following primary antibodies against the antigens were used: Tuj-1 (1:300, Promega), Synaptophysin (1:400 ...
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bioRxiv - Cancer Biology 2022Quote: ... Biotinylated antigens were immobilized using streptavidin-coated magnetic beads (Promega). In each round ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... HaloTag-GST was used as a negative control antigen (Promega).
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bioRxiv - Genetics 2022Quote: Type-1 quantitative bioluminescence resonance energy transfer (qBRET) experiments utilised the NanoBRET platform (Promega) and followed the general type-1 qBRET protocol reported by Felce et al.25 The assay was performed in HEK293 cells transiently expressing POPDC1 and POPDC2 constructs which possessed a C-terminal NanoLuc luciferase or HaloTag domains ...
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bioRxiv - Molecular Biology 2020Quote: ... NOCT antibodies were then purified from the verified serum by ammonium sulfate fractionation and then antigen affinity purification using recombinantly expressed HaloTag-NOCT (1-431) immobilized on HaloLink resin (Promega). The serum was purified over the immobilized NOCT column before elution with 5 M NaI and 1 mM ammonium thiocyanate ...
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bioRxiv - Cancer Biology 2019Quote: CHO K-1 cells overexpressing hPD-L1 and the recombinant TCR ligand (hPD-L1 Antigen Presenting Cells, hPD-L1 aAPCs, Promega) and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE ...
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bioRxiv - Immunology 2024Quote: ... Purified EDIII antigens were biotinylated using HaloTag PEG biotin ligand (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer with Azure Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer with Azure Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Biophysics 2020Quote: CosM6 cells were transfected with ∼1 µg of wild type or mutant constructs using FuGENE6 (Promega) and were patched within 1-2 days after transfection ...
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bioRxiv - Plant Biology 2020Quote: ... Antigen on the membrane was visualized with horseradish peroxidase-coupled anti-rabbit IgG (Promega) for VHA-a1 and VHA-a3 ...
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bioRxiv - Immunology 2021Quote: ... RBD and NTD antigens were site-specifically biotinylated using Halotag PEG biotin ligand (Promega), following the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... Antigen retrieval was performed by incubation with 20 μg/mL proteinase K (Promega, USA) for 20 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... selections were performed using biotinylated Fc fusion antigen captured on SA-coated magnetic beads (Promega). Prior to each selection ...
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bioRxiv - Cell Biology 2022Quote: ... other cell types were transfected using Fugene HD (Promega) or Lipofectamine 2000 (Life technologies ...
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bioRxiv - Microbiology 2022Quote: ... Both cassettes were built by first cloning the wild-type locus and 1 kb flanking region in the pGEM-T plasmid (Promega) and subsequently modifying the plasmid to add the HA-tag coding sequence using the Q5 Site-Directed Mutagenesis kit (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... The partial frame of circ_0005962 containing the binding site or mutant binding site (wild-type or mutant-type) with miR-382-5p was amplified and cloned into the downstream of pGL4 vector (Promega, Madison, WI, USA) to generate circ_0005962-wt and circ_0005962-mut fusion plasmids ...
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bioRxiv - Cell Biology 2022Quote: ... (ii) wild-type cells transfected with mEGFP using Fugene6 (Promega), (iii ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 25% confluent wells were transfected with 2 µg plasmid vector expressing SV40 antigen using Fugene Transfection Reagent (Promega) as described by manufacturer ...
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bioRxiv - Synthetic Biology 2022Quote: ... verified for cell type identity using the GenePrint 24 system (Promega, B1870), and confirmed as mycoplasma-free using a commercial testing kit every three months.
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bioRxiv - Cancer Biology 2022Quote: ... verified for cell type identity using the GenePrint 24 system (Promega, B1870), and confirmed as mycoplasma-free every three months using a commercial testing kit ...
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bioRxiv - Cancer Biology 2020Quote: ... Each plate contained at least one wild-type DNA control (Promega, #G152A).
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bioRxiv - Cell Biology 2023Quote: ... verified for cell type identity using the GenePrint 24 system (Promega, B1870), and confirmed as mycoplasma-free every three months using a commercial testing kit.
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bioRxiv - Microbiology 2020Quote: ... incubating and chromogenic reaction generating were similar to the ACE2 assay instead of that primary antibody binding to antigen was not required and that HRP-conjugated goat anti-human IgG antibody (Promega) would be used as the secondary antibody to detect binding of CB6 antibody to the antigens.
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bioRxiv - Bioengineering 2020Quote: Phage selections were done according to previously established protocols.30 Selections were performed using biotinylated antigens captured with streptavidin-coated magnetic beads (Promega). In each round ...
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bioRxiv - Cell Biology 2023Quote: ... wild-type and mutant tepsin-myc constructs were transfected using Fugene 4K (Promega) at 1.5:1 Fugene:DNA ratio following manufacturer protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in a 6-well plate and transfected with 10 µg of linearised plasmid expressing the SV40 Large T antigen using the FuGene Transfection Kit (Promega, #E2691) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... SEA (0.2 mg/ml) and soluble schistosomula antigens (0.2 mg/ml) was determined using the Caspase-Glo® 3/7 Assay System (Promega, Sydney, Australia) according to the manufacturers’ instructions.
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bioRxiv - Neuroscience 2019Quote: ... genomic DNA from puromycin selected cells and wild-type HEK293 cells was extracted (Promega Wizard Genomic DNA Purification Kit #A1120 ...
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bioRxiv - Genetics 2020Quote: Full-length wild-type and mutant Csde1 5’UTRs were inserted pGL3 (Promega, E1751) plasmid in between EcoRI and NcoI sites upstream of Firefly luciferase gene ...
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bioRxiv - Microbiology 2024Quote: ... the nLuc activity of each antigen was adjusted to comprise 107 RLU per second (RLU/s) in a volume of 50 µL (NanoGlow, Promega, Madison, Wisconsin, USA).
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bioRxiv - Synthetic Biology 2019Quote: The wild type and mutant promoter of miR-146a-5p were constructed into pGL3 vector (Promega). The pGL3-WT or pGL3-mut were co-transfected with pRL-TK using lipofectamine® 3000 ...
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bioRxiv - Biochemistry 2019Quote: Wild-type and mutant AP2ΔµC complexes were expressed in BL21 (DE3) pLysS strain (Promega, Madison, WI) by induction at 23 °C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... wild type or mutant 3′-UTR of DKK3 was cloned into the psicheck-2 vector (Promega). HCT116 cells were transfected with and wild-type or mutant 3′-UTR-luc by using Lipofectamine 3000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The wild-type and mutants of MCHR1 and MCHR2 were constructed into the pcDNA6.0 vector (Promega) for functional assays.
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bioRxiv - Cell Biology 2020Quote: DNA was extracted from each cell type with the Wizard Genomic DNA Purification Kit (Promega, Southampton, UK) and send for STR profiling (Core Genomic Facility ...
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bioRxiv - Plant Biology 2020Quote: ... oryzae (Pso)-treated 9-WT wild type and hac701 with Maxwell 16 LEV Plant RNA Kit (Promega) run on the Maxwell 16 Instrument (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... The transfection of wild-type MCF10A cells and the mutated derivatives was carried out with FuGene (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was isolated from 4-day-old wild-type seedlings using a Maxwell RSC Plant Kit (Promega). cDNA was synthesized from total RNA (100 ng ...
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PPARγ is a tumor suppressor in basal bladder tumors offering new potential therapeutic opportunitiesbioRxiv - Cancer Biology 2019Quote: ... Cells were transfected 24 h later with 2.5 µg of pRP-PPARG2 wild-type or mutated vector (Vector Builder) in the presence of Fugene HD transection reagent according to manufacturer’s instructions (Promega). The cells were selected 24h after transfection with 4µg/mL of puromycin for 24h ...
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PPARγ is a tumor suppressor in basal bladder tumors offering new potential therapeutic opportunitiesbioRxiv - Cancer Biology 2019Quote: ... These cells were transfected 24 h later with 2.5 µg of pcDNA3-PPARγ2 (wild-type or mutated) in the presence of the Fugene HD transfection reagent (Promega). PPARγ2 transient overexpression in the UMUC3 ...
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bioRxiv - Cancer Biology 2021Quote: ... wild-type and mutant MMP2 3′-UTRs were synthesized and recombined into pmirGLO Luciferase vectors (Promega, Madison, WI, USA). Then pmirGLO-Wt/pmirGLO-Mt or miR-1299 mimics/miR-NCs were co-transfected into EC9706/KYSE30 cells ...
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bioRxiv - Immunology 2021Quote: ... and several sample types were evaluated for ADCC and ADCP functionalities using the corresponding Reporter Bioassay kit (Promega Corporation). ADCC and ADCP induction was expressed as induction ratio (IR) ...
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bioRxiv - Plant Biology 2020Quote: ... PCRs were used to assay for wild-type or T-DNA insertion alleles in 20 μL reactions using GoTaq Green Master Mix (Promega) using manufacturer’s instructions and 30 cycles as follows ...
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bioRxiv - Microbiology 2021Quote: ... The wild-type and mutant miRNA target sites were cloned downstream of the Firefly luciferase (luc2) reporter gene in pmirGLO vector (Promega) for luciferase reporter assays ...
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bioRxiv - Synthetic Biology 2019Quote: The wild type and mutant 3’ UTR of BRCA1 were constructed into psi-check-2 vector (Promega, Madison, WI, USA). Cells were plated in 24-well plates and the plasmids (psi-check-2-WT ...
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bioRxiv - Cell Biology 2022Quote: ... RPE1 wild type cells were transfected with PX459 plasmid containing the corresponding guide sequence for 24 hours using FuGENE 6 (Promega). Following 72 hours of 4 μM Puromycin selection ...
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bioRxiv - Microbiology 2022Quote: In vitro translation assays of the wild type or mutant CrPV-1A RNAs were performed in Spodoptera frugiperda 21 (sf-21) insect cell extract (Promega). Briefly ...