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Citations for Promega :
1 - 50 of 127 citations for Adenovirus Type 11 Particles Wild type since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... (ii) wild-type cells transfected with mEGFP using Fugene6 (Promega), (iii ...
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bioRxiv - Cancer Biology 2020Quote: ... Each plate contained at least one wild-type DNA control (Promega, #G152A).
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bioRxiv - Cell Biology 2023Quote: ... wild-type and mutant tepsin-myc constructs were transfected using Fugene 4K (Promega) at 1.5:1 Fugene:DNA ratio following manufacturer protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The partial frame of circ_0005962 containing the binding site or mutant binding site (wild-type or mutant-type) with miR-382-5p was amplified and cloned into the downstream of pGL4 vector (Promega, Madison, WI, USA) to generate circ_0005962-wt and circ_0005962-mut fusion plasmids ...
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bioRxiv - Neuroscience 2019Quote: ... genomic DNA from puromycin selected cells and wild-type HEK293 cells was extracted (Promega Wizard Genomic DNA Purification Kit #A1120 ...
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bioRxiv - Genetics 2020Quote: Full-length wild-type and mutant Csde1 5’UTRs were inserted pGL3 (Promega, E1751) plasmid in between EcoRI and NcoI sites upstream of Firefly luciferase gene ...
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bioRxiv - Biophysics 2020Quote: CosM6 cells were transfected with ∼1 µg of wild type or mutant constructs using FuGENE6 (Promega) and were patched within 1-2 days after transfection ...
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bioRxiv - Synthetic Biology 2019Quote: The wild type and mutant promoter of miR-146a-5p were constructed into pGL3 vector (Promega). The pGL3-WT or pGL3-mut were co-transfected with pRL-TK using lipofectamine® 3000 ...
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bioRxiv - Biochemistry 2019Quote: Wild-type and mutant AP2ΔµC complexes were expressed in BL21 (DE3) pLysS strain (Promega, Madison, WI) by induction at 23 °C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... wild type or mutant 3′-UTR of DKK3 was cloned into the psicheck-2 vector (Promega). HCT116 cells were transfected with and wild-type or mutant 3′-UTR-luc by using Lipofectamine 3000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The wild-type and mutants of MCHR1 and MCHR2 were constructed into the pcDNA6.0 vector (Promega) for functional assays.
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bioRxiv - Plant Biology 2020Quote: ... oryzae (Pso)-treated 9-WT wild type and hac701 with Maxwell 16 LEV Plant RNA Kit (Promega) run on the Maxwell 16 Instrument (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... The transfection of wild-type MCF10A cells and the mutated derivatives was carried out with FuGene (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was isolated from 4-day-old wild-type seedlings using a Maxwell RSC Plant Kit (Promega). cDNA was synthesized from total RNA (100 ng ...
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PPARγ is a tumor suppressor in basal bladder tumors offering new potential therapeutic opportunitiesbioRxiv - Cancer Biology 2019Quote: ... These cells were transfected 24 h later with 2.5 µg of pcDNA3-PPARγ2 (wild-type or mutated) in the presence of the Fugene HD transfection reagent (Promega). PPARγ2 transient overexpression in the UMUC3 ...
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bioRxiv - Cancer Biology 2021Quote: ... wild-type and mutant MMP2 3′-UTRs were synthesized and recombined into pmirGLO Luciferase vectors (Promega, Madison, WI, USA). Then pmirGLO-Wt/pmirGLO-Mt or miR-1299 mimics/miR-NCs were co-transfected into EC9706/KYSE30 cells ...
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bioRxiv - Plant Biology 2020Quote: ... PCRs were used to assay for wild-type or T-DNA insertion alleles in 20 μL reactions using GoTaq Green Master Mix (Promega) using manufacturer’s instructions and 30 cycles as follows ...
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bioRxiv - Synthetic Biology 2019Quote: The wild type and mutant 3’ UTR of BRCA1 were constructed into psi-check-2 vector (Promega, Madison, WI, USA). Cells were plated in 24-well plates and the plasmids (psi-check-2-WT ...
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bioRxiv - Cell Biology 2022Quote: ... RPE1 wild type cells were transfected with PX459 plasmid containing the corresponding guide sequence for 24 hours using FuGENE 6 (Promega). Following 72 hours of 4 μM Puromycin selection ...
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bioRxiv - Microbiology 2022Quote: ... Both cassettes were built by first cloning the wild-type locus and 1 kb flanking region in the pGEM-T plasmid (Promega) and subsequently modifying the plasmid to add the HA-tag coding sequence using the Q5 Site-Directed Mutagenesis kit (NEB ...
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bioRxiv - Microbiology 2022Quote: In vitro translation assays of the wild type or mutant CrPV-1A RNAs were performed in Spodoptera frugiperda 21 (sf-21) insect cell extract (Promega). Briefly ...
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bioRxiv - Genetics 2020Quote: ... Using the primers provided in Supplementary Table 3 we performed PCRs on 40 ng of genomic material extracted from wild type mosquitoes of the G3 strain using the Wizard Genomic DNA purification kit (Promega). The primers were modified to contain suitable Gibson assembly overhangs (underlined ...
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bioRxiv - Molecular Biology 2020Quote: ... or R602P) and Cbs-pCMV-sport6 (either wild type or I278T) were transfected into the cells with Fugene HD (Promega). Cells were incubated for 48 hours and then DMEM was removed ...
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bioRxiv - Pathology 2020Quote: Reporter vectors were generated by inserting the Fam210a 3’UTR fragments containing wild-type or mutated seed sequence into the miRNA reporter vector pmirGlo (Promega). All the primers used to generate the reporter vector were listed in supplemental information ...
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bioRxiv - Biochemistry 2022Quote: ... GS-mediated GS-cAMP accumulation assays were performed with HEK293T (ATCC CRL-11268) cells transiently expressing human D1R or D5R wild-type and mutant along with the cAMP biosensor GloSensor-22F (Promega). Cells were seeded (20 000 cells/35 μL/well ...
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bioRxiv - Developmental Biology 2022Quote: HEK293 cells cultured on 10cm dishes were transfected with 10 μg of pLVTHM or pHAGE2 vectors under the control of an EF1α promoter and containing the wild-type or mutant versions of Oct4 or Sox2 with Fugene6 (Promega) using a low volume protocol (Steffen et al. ...
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bioRxiv - Cell Biology 2022Quote: A full length TNY1 cDNA was amplified with primers TNY1-1F and TNY1-1R (Supplementary Table) from cDNA prepared using RNA from wild-type strain CC124 and inserted into pGEM-T easy vector (Promega) to generate pGEM-TNY1 ...
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bioRxiv - Biochemistry 2023Quote: ... stable HEK293T cells expressing wild type COMT were transiently transfected with pTagBFP-C expressing the KDEL marker using FugeneHD (Promega) following the manufacturer’s instructions with 100 ng plasmid ...
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bioRxiv - Molecular Biology 2023Quote: The sequences of wild-type (WT) F8 exons and 100-250 nucleotides flanking each exon were amplified from human genomic DNA (Promega) using WT PCR primers shown in Supplemental Table 1 ...
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bioRxiv - Cancer Biology 2021Quote: MAGI2-AS3 or FOXN3 3’UTR containing miR-452-5p wild-type (WT) or mutant (MUT) binding sites were inserted into psiCHECK vector (Promega, USA). 293T cells were transfected with the constructed luciferase plasmid together with miR-NC or miR-452-5p mimics using Lipofectamine 2000 ...
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bioRxiv - Developmental Biology 2020Quote: ... Required fragments were PCR-amplified from wild-type genomic DNA and used as template for preparation of DIG-labeled probe using T7 RNA Polymerase (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... the luciferase activity for wild type and mutant pseudoviruses were determined using Steady-Glo luciferase assay system (Promega Corp; Madison; WI, USA), and luminescence assessed with a Glomax Multi Detection System (Promega).
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bioRxiv - Molecular Biology 2021Quote: ... RPGR wild-type minigene construct was generated (Figure 2A) by amplification from a pool of human female genomic DNAs (Promega, Milan, Italy) using the following primers:
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bioRxiv - Neuroscience 2019Quote: ... genomic DNA from puromycin selected cells and wild-type HEK293 cells was extracted (Promega Wizard Genomic DNA Purification Kit #A1120; Promega, Madison, WI, USA) for PCR amplification of a 535 bp region flanking the sgRNA cleavage site ...
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bioRxiv - Systems Biology 2019Quote: ... The transfection (of wild-type HeLa cells with the HSF1:eGFP plasmid) was carried out using FuGENEï¿œ HD transfection reagent (Promega, Charbonnières, France) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The wild-type (wt) or mutant (mut) 3’-UTR sequences of XIST or SMAD2 was cloned into pmirGLO vector (Promega, Madison, WI, USA). The pGL3-XIST-wt or XIST-mut and pmirGLO-SMAD2-wt or SMAD2-mut reporter vector were co-transfected with miR-34a mimics and NC mimics into SRA01/04 cells using Lipofectamine 2000 (Invitrogen) ...
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Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2023Quote: HEK293 cells growing at 70% confluency in a 10 cm dish were transfected with wild-type human NOPR or NOPLight (3 µg DNA) and GloSensor-20F (Promega, 2.5 µg DNA) using 12 µL Lipofectamine 2000 (Thermo Fisher ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from overnight bacteria cultures from resistant and wild type parental strains using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, USA), quantified and measured for DNA quality by Qubit High Sensitive dsDNA assay (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... other cell types were transfected using Fugene HD (Promega) or Lipofectamine 2000 (Life technologies ...
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bioRxiv - Synthetic Biology 2022Quote: ... verified for cell type identity using the GenePrint 24 system (Promega, B1870), and confirmed as mycoplasma-free using a commercial testing kit every three months.
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bioRxiv - Cancer Biology 2022Quote: ... verified for cell type identity using the GenePrint 24 system (Promega, B1870), and confirmed as mycoplasma-free every three months using a commercial testing kit ...
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bioRxiv - Cell Biology 2023Quote: ... verified for cell type identity using the GenePrint 24 system (Promega, B1870), and confirmed as mycoplasma-free every three months using a commercial testing kit.
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bioRxiv - Genetics 2023Quote: High molecular weight DNA was isolated from spleen tissue of a single male from each of the 11 imported Nachman wild-derived inbred strains using the Wizard DNA Purification Kit (Promega) or the Monarch HMW DNA kit (NEB ...
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bioRxiv - Genetics 2022Quote: Type-1 quantitative bioluminescence resonance energy transfer (qBRET) experiments utilised the NanoBRET platform (Promega) and followed the general type-1 qBRET protocol reported by Felce et al.25 The assay was performed in HEK293 cells transiently expressing POPDC1 and POPDC2 constructs which possessed a C-terminal NanoLuc luciferase or HaloTag domains ...
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bioRxiv - Cell Biology 2020Quote: DNA was extracted from each cell type with the Wizard Genomic DNA Purification Kit (Promega, Southampton, UK) and send for STR profiling (Core Genomic Facility ...
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bioRxiv - Biochemistry 2024Quote: Streptavidin paramagnetic particles (Promega), pre-equilibrated in wash buffer (20 mM HEPES pH 7.4 ...
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PPARγ is a tumor suppressor in basal bladder tumors offering new potential therapeutic opportunitiesbioRxiv - Cancer Biology 2019Quote: ... Cells were transfected 24 h later with 2.5 µg of pRP-PPARG2 wild-type or mutated vector (Vector Builder) in the presence of Fugene HD transection reagent according to manufacturer’s instructions (Promega). The cells were selected 24h after transfection with 4µg/mL of puromycin for 24h ...
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bioRxiv - Immunology 2021Quote: ... and several sample types were evaluated for ADCC and ADCP functionalities using the corresponding Reporter Bioassay kit (Promega Corporation). ADCC and ADCP induction was expressed as induction ratio (IR) ...
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bioRxiv - Microbiology 2021Quote: ... The wild-type and mutant miRNA target sites were cloned downstream of the Firefly luciferase (luc2) reporter gene in pmirGLO vector (Promega) for luciferase reporter assays ...
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bioRxiv - Neuroscience 2021Quote: ... Streptavidin MagneSphere paramagnetic particles (Promega) were first washed in EMSA buffer 1X (EMSA buffer 2X ...