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Citations for Promega :
2901 - 2920 of 2920 citations for 7 Phenyl 1 heptanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Samples were diluted in 50 mM ammonium bicarbonate and normalized to 50 μg protein for overnight LysC/trypsin digestion (Promega, protein:enzyme ratio, 50:1). Trifluoroacetic acid (TFA ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary HRP-conjugated antibodies were added at a dilution of 1:10,000 in 5% milk in TBST and incubated for 60 minutes at room temperature (Anti-Mouse: Promega #W402B; Anti-Rabbit: Promega #W401B). The Pierce SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Scientific #34577 ...
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bioRxiv - Cell Biology 2023Quote: ... pH 8.5 to a final urea concentration of 2 M for Trypsin/Lys-C based overnight protein digestion at 37 °C (40 µg of protein used for global proteomics and the remainder for phosphoproteomics, 1:70 protease: substrate ratio, Mass Spectrometry grade, Promega Corporation, Cat No: V5072.) Digestions were acidified with trifluoroacetic acid (TFA ...
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bioRxiv - Immunology 2023Quote: ... stable HEK293 EBNA cell lines were generated using the sleeping beauty transposon system.91 Expression constructs were co-transfected with a transposase plasmid (10:1) into the HEK293 EBNA cells using FuGENE® HD transfection reagent (Promega GmbH, Madison, USA) in DMEM/F12 supplemented with 6% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted with ABC to a final concentration of 1 M urea and digested using trypsin (Promega, V5113; 3 μg/mL, 37°C, 16 h). After digestion ...
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bioRxiv - Plant Biology 2023Quote: ... The proteins were separated by 12% Tris–Glycine– SDS–PAGE and the gel was electroblotted to a PVDF membrane (0.2 µm) followed by immunodetection using Anti-HaloTag® Monoclonal Antibody (1:5000) (Promega, Madison, Wisconsin, United States) in Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2023Quote: ... the presence of other main circulating arboviruses was evaluated by RT-qPCR using GoTaq® 1-Step RT-qPCR System (Promega, Madison, WI, USA). Specific diagnosis primers were used for Zika [30] ...
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bioRxiv - Biochemistry 2024Quote: ... the amplified PCR products were digested using the corresponding restriction enzymes (Supplementary Table 1) and then ligated into similarly digested pYES2 using LigaFast Rapid Ligation System (Promega Corporation, Madison, WI, USA) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900 μL of 0.35 M KCl (10 mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
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bioRxiv - Cell Biology 2021Quote: ... 30 min at 60°C) an alkylation (IAA 0.5M, 30 min RT) microtubule-associated protein enriched fractions were digested using trypsin (Gold, Promega, 1 μg / sample, overnight at 30°C). Peptide clean-up was done using OMIX C18 (Agilent ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900μL of 0.35M KCl (10mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900μL of 0.35M KCl (10mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
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bioRxiv - Microbiology 2020Quote: ... alkylation was performed using 20 mM 2-chloroacetamide at room temperature in HEPES buffer for 30 min under exclusion of light. Samples were prepared according to the SP3 protocol (Hughes et al. 2019) and trypsinized (sequencing grade, Promega, enzyme to protein ratio 1:50) overnight at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Each organ was homogenized with a lysis solution containing a 1:5 dilution of Luciferase Cell Culture Lysis 5X Reagent (Promega, Madison, WI, USA, cat. no. E1531) in Millipore water supplemented with 1 % (v/v ...
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bioRxiv - Developmental Biology 2024Quote: ... The membranes were washed 3x 10 minutes in TBS-T before being incubated for 2 hours with the secondary anti-mouse-HRP- or anti-rabbit HRP antibody (1:5000, Promega anti-mouse, W4021; anti-rabbit W4011). The membranes were washed in TBS-T for 3x 10 minutes and developed with Western Lightning Plus-ECL reagent (Perkin Elmer NEL104) ...
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bioRxiv - Microbiology 2020Quote: ... for 3 hours before being diluted with 5 volumes of 40 mM NH4HCO3 and digested overnight with sequencing grade modified trypsin (Promega, Madison, WI, USA; 1/50 w/w). Digested samples were acidified to a final concentration of 0.5% formic acid and desalted with 50 mg tC18 Sep-Pak columns (Waters corporation ...
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bioRxiv - Cell Biology 2019Quote: ... Cell signalling) or rabbit anti-TgTOM40 (1:2000, (van Dooren et al., 2016)) antibodies coupled to secondary horseradish peroxidase (HRP)(Promega for mouse and rabbit, Abcam for rat) conjugated antibodies (1:10,000 ...
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bioRxiv - Biochemistry 2021Quote: ... The dried resin was resuspended with 50 mM Tris pH 7.5 (2x resin volume) and 200 ng of trypsin was added (Promega; made up to 100 ng·μL-1 in H2O), the samples were digested overnight shaking at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Light signals derived from reconstituted HiBiT/LgBiT association were measured using Tristar multiwell luminometer (Berthold Technology, Bad Wildbad, Germany) for 2.5 seconds by adding 20 µl of Nano-Glo® substrate (Promega Inc, WI, USA; diluted 1:40 in PBS). Mock-infected cells served as controls to determine basal luciferase activity for obtaining normalized relative light units in infected samples ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then incubated at 37 °C in tissue culture incubator for 4 hours or 24 hours before measuring reconstituted HiBiT/LgBiT activity using a Tristar multiwell Luminometer (Berthold Technology, Bad Wildbad, Germany) for 2.5 seconds by adding 20 µl of Nano-Glo® substrate (Promega Inc, WI, USA; diluted 1:40 in PBS). Individual unmixed populations of cells were treated identically and served as controls to determine basal luciferase activity to obtain normalized relative light units ...