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Citations for Promega :
2201 - 2250 of 4574 citations for 6 CHLORO 2 METHYLIMIDAZO 1 2 A PYRIDINE 3 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Apoptosis induction in response to SRRM1 silencing in leukemia cell lines (25,000 cells/well onto white-walled multiwell luminometer plates) was performed by using Caspase-Glo® 3/7 Assay (Promega Corporation, #G8091) as previously reported (67) ...
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bioRxiv - Cancer Biology 2019Quote: ... These were transfected into Tet-On HEK-293TLDcells [10] by incubating 3 µg plasmid DNA with 9 µL Fugene HD (Promega, Madison, WI) in 100 µL Optimem for 15 min ...
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bioRxiv - Biochemistry 2021Quote: The promoter fragment of luciferase reporter plasmid pGADD34-0.5k generated by Eurofins Japan (Tokyo, Japan) was subcloned into a pGL-3 vector (Promega, Madison, WI, USA) by restriction enzyme cutting site KpnI/HindIII ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 24 clones (4 holobionts x 3 replicates x 24 clones = three 96-well plates) from each amplicon were amplified directly with GoTaq polymerase (Promega, Lyon France) in a 25 μL reaction volume using the following PCR parameters ...
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bioRxiv - Cell Biology 2022Quote: ... Control and treated cells were cultured for 1 day in 96-well plates then treated with small molecules for 3 consecutive days before incubation with 120 μL fresh media containing 20 μL of CellTiter 96® AQueous One Solution (Promega, G3580) for 1 to 4 hours at 37°C in a humidified ...
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bioRxiv - Molecular Biology 2022Quote: ... SEA (0.2 mg/ml) and soluble schistosomula antigens (0.2 mg/ml) was determined using the Caspase-Glo® 3/7 Assay System (Promega, Sydney, Australia) according to the manufacturers’ instructions.
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bioRxiv - Microbiology 2023Quote: The quantification of viral RNA was carried out in one-step RT-qPCR using 3 µl of the eluted RNA with the GoTaq® Probe One-step RT-qPCR System (Promega, Poland). This procedure employed in-house primers and a probe specifically tailored for the HCoV-229E N sequence ...
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bioRxiv - Evolutionary Biology 2023Quote: The 419 bp sequence of 3’UTR containing predicted target sites for miR-252 in CcTRPM was cloned into the pmirGLO vector (Promega, Wisconsin, USA) downstream of the luciferase gene to construct the recombinant plasmid of CcTRPM-3’UTR-pmirGLO using the pEASY®-Basic Seamless Cloning and Assembly Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... tissues were incubated with primary antibodies diluted in 10% DS in PB1 for 3 days at 4°C followed by four washes over the following day with 0.2% Tween-20 (Promega UK Ltd, H5151) in PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... tissue was immediately incubated in 1% PFA (with 1 µL mL−1 RNasin Plus (Promega, cat. #N2611)) for 5 minutes at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... A polyclonal goat anti-mouse conjugated to horseradish peroxidase (1:5000, 1:10000 or 1:20000; Promega) was used as secondary antibody ...
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bioRxiv - Biophysics 2019Quote: ... DBNL(1-256) or DBNL(1-374) using ViaFect (Promega) and assayed 24 h later ...
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bioRxiv - Immunology 2020Quote: ... and mixed 1:1 v:v with BacTiter-Glo Reagent (Promega). The reaction was incubated for 5 min at room temperature on an orbital shaker ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM dNTP) and 1 μl of AMV RT (Promega) was added and the sample was incubated at 42 °C for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were lysed in 1:1 PBS:CellTiter-Glo 2.0 (Promega) reagent ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was diluted 1:1 with nuclease-free water (Promega). Quantitative real-time PCR reactions were carried out using the SYBR Green PCR master mix and ROX Passive Reference Dye (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... and a 1:1 mixture of CellTiter-Glo reagent (Promega) and PBS was added to each well ...
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bioRxiv - Developmental Biology 2021Quote: ... 1% Digitonin (Promega) and EDTA-free Protease Inhibitor cocktail (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... 1) Trypsin (Promega) and Lys-c (Wako) ...
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bioRxiv - Developmental Biology 2020Quote: ... 1/200 (Promega). The epidermis of WPP was dissected following fillet preparation protocols described in 32.
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bioRxiv - Developmental Biology 2020Quote: ... (1:2000, Promega). Secondary antibodies (diluted 1:200 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1:2,000 (Promega); anti-FtsZ ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% Digitonin (Promega) and 50x EDTA-free Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... The 3’ UTR fragment was digested with Xba I and inserted downstream of the firefly luciferase gene in pGL3-control (Promega, Madison, WI, USA), yielding pGL3covi-3UTR_Luc ...
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bioRxiv - Genetics 2019Quote: Buccal cell samples were collected and prepared as for the standard processing method with the exception that 3 μl 5 x AmpSolution™ (Promega Corporation, USA), was added to the PCR reagents in place of low TE buffer ...
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bioRxiv - Cell Biology 2021Quote: ... of SNHG16 sequence and the 3’-untranslated region (UTR) fragment of STARD9 containing miR-1301-3p binding site were subcloned into the pmirGLO vectors (Promega, Madison, WI, USA) to generate SNHG16-Wt/Mut vectors and STARD9-Wt/Mut vectors ...
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bioRxiv - Bioengineering 2019Quote: ... beads were equilibrated in 100 mM ammonium bicarbonate with 3 washes prior to on-bead digestion with trypsin (800 ng; Promega cat. nr. V5280) overnight at 37°C 800 rpm ...
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bioRxiv - Microbiology 2021Quote: ... The wild-type (wt) or mutant (mut) 3’-UTR sequences of XIST or SMAD2 was cloned into pmirGLO vector (Promega, Madison, WI, USA). The pGL3-XIST-wt or XIST-mut and pmirGLO-SMAD2-wt or SMAD2-mut reporter vector were co-transfected with miR-34a mimics and NC mimics into SRA01/04 cells using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... CDC was measured after incubation for 3 hours at 37 °C 5% CO2 with a luminometer using the CytoTox-Glo Cytotoxicity Assay (Promega; Cat. Nr.: G9291) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... 100 uL of the diluted lysate was added to each well along with an equal volume of Caspase Glo 3/7 assay reagent (Promega, Madison, Wisconsin, USA), according to the manufacturer’s directions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The amplicons generated from the 5’ and 3’ race PCRs were subjected to purification and subsequent cloning into the pGEM-T easy vector (Promega Corp., WI, USA). The constructed vectors were sequenced (Plasmidsaurus ...
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bioRxiv - Microbiology 2023Quote: ... The copy numbers of the control DNA/RNA were calculated based on the molecular weight and absorbance at 260 nm of RNA that had been serially diluted (≥3 steps) and/or fluorescence using the Quantus Fluorometer (Promega, Madison, WI, USA) (n = 3).
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bioRxiv - Microbiology 2023Quote: ... After five-fold dilution with 50-mM ammonium bicarbonate, the sample was digested using Lys-C (Wako, Osaka, Japan) at 37°C for 3 h following trypsin (Promega, Madison, WI, USA) at 37°C for 16 h ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... membrane integrity (CytoTox-OneTM homogeneous membrane integrity assay kit: lactate dehydrogenase (LDH release) and caspase activity/ apoptosis (Caspase-Glo® 3/7 assay system kit) (all from Promega, WI, USA), as described previously17 (see Supplementary data for detailed description).
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: HEK293 cells growing at 70% confluency in a 10-cm dish were transfected with wild-type human NOPR or NOPLight (3 μg DNA) and GloSensor-20F (Promega, 2.5 μg DNA) using 12 μL Lipofectamine 2000 (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: The Luciferase reporter vector were constructed via insertion of uc.110 conserved region and 3’UTR of MFRP downstream of Renilla luciferase stop codon in psi-CHECK2 dual luciferase vectors (Promega, Madison, WI, USA). The insertions were validated by sequencing ...
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bioRxiv - Microbiology 2020Quote: ... duplicate wells were transfected with 1 µg HDM_Spike_RBD_B7-1 plasmids and 1 µg of Transfection Carrier DNA (Promega, E4881) using BioT reagent (Bioland Sci ...
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bioRxiv - Molecular Biology 2023Quote: ... A polyclonal goat anti-mouse conjugated to horseradish peroxidase (1:5,000; 1:10,000; or 1:20,000; Promega, W4021) was used as the secondary antibody ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were treated with 1 μg ml-1 Proteinase K (Promega) between 1 and 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM CaCl2 and 1 ug of sequence-grade trypsin (Promega) were added and incubation continued overnight ...
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bioRxiv - Neuroscience 2020Quote: ... a caspase-1 selective inhibitor Ac-YVAD-CHO (1 μM; Promega) was added in parallel experiments ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM dNTP) and 1 μl of AMV reverse transcriptase (Promega) was then added and extension was carried out for 1 h at 42 °C ...
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bioRxiv - Microbiology 2024Quote: ... Subsequently Trypsin/LysC solution [100 ng/μL 1:1 Trypsin (Promega):LysC (FujiFilm ...
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bioRxiv - Cancer Biology 2023Quote: ... each well was treated with 1:1 CellTiter-Glo (Promega, UK) and incubated for 10mins at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... 1% RNAse-free BSA (Gemini) and 1:400 RNasin Plus (Promega) before storage at -80°C ...
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bioRxiv - Microbiology 2023Quote: ... 8μl of Trypsin/LysC solution (100ng/μl 1:1 Trypsin (Promega) and LysC (FujiFilm ...
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bioRxiv - Neuroscience 2023Quote: ... 8μl of Trypsin/LysC solution (100ng/μl 1:1 Trypsin (Promega) and LysC (FujiFilm ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 250 µL (1:1 volume) of One-Glo reagent (E6110, Promega) was added to each well and incubated for 10 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 75 µL (1:1 volume) of One-Glo reagent (E6110, Promega) was added to each well and incubated for 10 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... followed by three washes with TBS-T and 1 hr incubation in TBS-T + 1% milk + anti-mouse serum (1:10,000) (Promega). All steps were carried out at room temperature ...