Labshake search
Citations for Promega :
1051 - 1100 of 1295 citations for Recombinant Canine KITLG Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... Protein samples were precipitated with 0.1 M ammonium acetate in 100% methanol and the protein pellets were further digested with sequencing-grade trypsin (Promega). For the analysis involving the rpsAΔ6 mutant missing the last C-terminal OB fold domain of protein S1 ...
-
bioRxiv - Microbiology 2021Quote: ... Following the dilution of the sample with 100 mM ammonium bicarbonate to a final urea concentration of 800 mM the proteins were digested further with 0.2 µg/µl trypsin (Promega) (37 °C ...
-
bioRxiv - Microbiology 2021Quote: ... The SK-N-SH cell surface proteins with attached TBEV virions were digested off with 1.25 μg trypsin (Promega) and the supernatant collected ...
-
bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 spike protein (23) into HEK 293T cells using the FuGENE 6 Transfection Reagent (Promega). Spike sequences from the following global strains REF were used ...
-
bioRxiv - Microbiology 2021Quote: ... 0.025% deoxycholate and 200 µg.mL−1 RNase A at 37°C for 20 min to lyse the cells and Protein Precipitation Solution (Promega) was added ...
-
bioRxiv - Molecular Biology 2022Quote: ... Chromatin-bound proteins were solubilised by incubation with 1320 U of DNase I (RQ1 RNase-free DNase I, Promega) on ice for 20 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... The proteins in the sample were digested with 10 μg/mL sequence grade modified trypsin (Promega, Madison, WI, USA) at 37°C overnight ...
-
bioRxiv - Neuroscience 2020Quote: A total of 100 μg protein was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation methods [19] ...
-
bioRxiv - Genetics 2022Quote: ... Protein-bound beads were washed in 80% ethanol and proteins were digested off the beads by addition of 0.8ug sequencing grade modified trypsin (Promega) in 100mM Ammonium bicarbonate ...
-
bioRxiv - Cancer Biology 2022Quote: ... IAA was neutralized by adding 10mM DTT final concentration and proteins were digested with 300ng of trypsin (Promega V5280) for 18h ...
-
bioRxiv - Cell Biology 2019Quote: ... and proteins were finally subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega, Madison, WI). The digested proteins were desalted using AssayMap C18 cartridges mounted on a BRAVO liquid handling system (Agilent ...
-
bioRxiv - Biochemistry 2020Quote: ... N-glycans were released from the intact proteins using 0.5 U/ µL N-glycosidase F (PNGase F, Elizabethkingia miricola, Promega), overnight ...
-
bioRxiv - Cell Biology 2019Quote: ... the protein sample was digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) overnight at 37°C ...
-
bioRxiv - Biochemistry 2019Quote: ... All samples were further diluted with 20 mM HEPES pH 8.0 to a final urea concentration of 2 M and proteins were digested with 5 μg trypsin (V5111, Promega) (1/100 ...
-
bioRxiv - Cancer Biology 2019Quote: ... Indicated amounts of HMGA2 and mutant HMGA2 protein were incubated with 100ng of negatively supercoiled Renilla reporter plasmid (Promega) for 30mins at 37°C in a buffer containing 10mM Tris-HCl ...
-
bioRxiv - Plant Biology 2019Quote: ... In-gel protein digestion was performed overnight at 37°C with trypsin (Sequencing Grade Modified Trypsin, Promega, Madison, USA) at a final concentration of 0.005 µg/µL ...
-
bioRxiv - Molecular Biology 2020Quote: ... ZmCLA4 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260) (Promega), and then purified using Magne HaloTag Beads (G7281 ...
-
bioRxiv - Cell Biology 2020Quote: ... Alkylation was quenched by the addition of 3 mM DTT and proteins were digested overnight at 37°C with 1 μg trypsin (0.5 μg/μl; Promega). Following digestion ...
-
bioRxiv - Immunology 2020Quote: ... The following day the cells were transfected with plasmids encoding the indicated FLAG-tagged protein (2 μg) with FuGENE HD according to manufacturer’s instructions (Promega). After 48 h cells were harvested and washed 3 times with PBS ...
-
bioRxiv - Cell Biology 2021Quote: ... Alkylation was quenched by the addition of 3 mM DTT and proteins were digested overnight at 37°C with 1 μg trypsin (0.5 μg/μl; Promega). Following digestion ...
-
bioRxiv - Genetics 2020Quote: ... Protein extracts were obtained from in vitro coupled transcription/translation systems (TNT® T7 Coupled Reticulocyte Lysate Systems, Promega). 15 µl of Flag-tagged proteins from TNT assays were pre-incubated on ice for 10 minutes in modified DBB (DBB ...
-
bioRxiv - Cancer Biology 2020Quote: ... and the protein pellet was resuspended in 50 mM ammonium bicarbonate and digested with sequencing-grade modified trypsin (Promega) overnight at 37°C ...
-
bioRxiv - Physiology 2022Quote: ... The proteins were cleaved into tryptic peptides by addition of 25 μl of a 0.2 μg/μl trypsin (Promega) in 50 mM ammonium bicarbonate and incubation (18 h ...
-
bioRxiv - Plant Biology 2022Quote: ... ZmEREB57 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260) (Promega), and then purified using Magne HaloTag Beads (G7281 ...
-
bioRxiv - Cancer Biology 2024Quote: ... The identity of each cell line was confirmed by DNA fingerprinting via short tandem repeats at the time of mRNA and total protein lysate preparation using the PowerPlex 1.2 kit (Promega).
-
bioRxiv - Molecular Biology 2023Quote: ... Bands corresponding to the protein of interest were cut from the gel and in-gel digestion with trypsin (Promega) was performed essentially as described in 41 ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... we dissolved 10 μg of protein in 10 μl 10 M urea containing 0.1% ProteasMax (Promega. Madison, WI, USA). Cysteine residues were reduced with 5 mM DTT (30 min at 50 °C ...
-
bioRxiv - Systems Biology 2023Quote: ... Samples were diluted 1:3 with 50 mM HEPES pH 8.5 and then digested by a 1:50 (trypsin to protein) ratio of sequencing grade modified trypsin (Promega) for 16 hours at 600 rpm and 25°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Beads were rinsed using 80% EtOH and proteins were digested with 100 µL of trypsin in 50 mM TEAB pH 7.55 (Promega, protein:enzyme ratio 1:50 ...
-
bioRxiv - Molecular Biology 2023Quote: Electrophoretic Mobility Shift Assays were performed using proteins expressed in the TNT T7 Coupled Reticulocyte Lysate System (Promega, WI), according to the manufactureŕs recommendation ...
-
bioRxiv - Cancer Biology 2023Quote: ... Unlabeled proteins were cell-free-synthesized using the TNT T7 Quick Reaction system according to the manufacturer instructions (Promega) and incubated with purified GST fusion proteins coupled with glutathione sepharose 4B fast flow beads (GE healthcare ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were further diluted with 50 mM Tris pH 7.9 pH 8.0 to a final urea concentration of 2 M and proteins were digested with 5 µg trypsin (Promega) (1/100 ...
-
bioRxiv - Molecular Biology 2022Quote: ... 50 ng/well of chaperone RTP1S,105 50 ng/well of the G protein subunit Gαolf,106–107 olfactory G protein subunit Gγ13,108 and 50 ng/well of pGloSensorTM-22F (Promega, Madison, USA).109 The utilized pGloSensor™-22F is a genetically engineered luciferase with a cAMP-binding pocket ...
-
bioRxiv - Genetics 2023Quote: ... proteins were extracted by chloroform-methanol precipitation and digested with 2 μg trypsin/Lys-C mixture (Promega, Madison, WI). Samples were labeled with 10-plex tandem mass tag reagents (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... urea was diluted to 1 M and proteins digested overnight with modified sequencing grade trypsin (Promega, Madison, WI, USA) at a 1:50 enzyme:protein ratio ...
-
bioRxiv - Plant Biology 2024Quote: ... proteins that underwent in vitro reactions were digested in 50 mM ammonium bicarbonate (NH4HCO3) with 0.2 µg trypsin (Promega) at 37°C overnight ...
-
bioRxiv - Plant Biology 2024Quote: ... and αKNL2-CΔDNAb (1,2) fused with a FLAG tag were used for protein expression with TNT SP6 High Yield Wheat Germ (Promega) following the manufacture’s protocol ...
-
bioRxiv - Microbiology 2024Quote: ... The sample was processed according to the SP3 protocol36 and digested overnight with trypsin (Promega, enzyme/protein 1:50). Peptides were desalted using SBD-RPS tips as previously described37 ...
-
bioRxiv - Molecular Biology 2024Quote: ... pH 8.0 to an urea concentration of 4 M and proteins were digested with 40 µg endopeptidase LysC (Promega) (1/250 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Samples were further diluted with 20 mM HEPES pH 8.0 to a urea concentration of 2 M and proteins were digested with 50 µg trypsin (Promega) (1/200 ...
-
bioRxiv - Plant Biology 2020Quote: HALO-FDL1 and HALO-GST (negative control) DAP-seq samples were performed using protein expressed in the TNT rabbit reticulocyte expression system (Promega) as previously described (Bartlett et al. ...
-
bioRxiv - Biophysics 2020Quote: Plasmid DNAs containing Tol2 vectors comprising GV genes and fluorescent protein genes described above and transposase mRNA were introduced to KPL-4 cells by lipofection using FuGene HD (Promega) in OptiMEM (Gibco) ...
-
bioRxiv - Developmental Biology 2020Quote: In vitro translation (IVT) with 500 ng of mRNA was performed using the TnT SP6 High-Yield Protein Expression System (Promega) to synthesize tagged proteins ...
-
bioRxiv - Molecular Biology 2019Quote: ... sliced and dehydrated twice using 50% ACN followed by 100% ACN and the proteins digested with ~100 µl of Trypsin solution (10 µg/ml, Promega) in 25 mM ammonium bicarbonate by incubating overnight at 37° C ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells expressing HT-fusion proteins or HT-Ctrl were incubated in the mammalian lysis buffer supplemented with Protease Inhibitor cocktail (Promega) and RQ1 RNase-Free DNase (Promega ...
-
bioRxiv - Neuroscience 2019Quote: ... Luciferase assays (Firefly and Renilla) were done on the protein extracts according to manufacturer instructions (Dual-Luciferase Reporter Assay System, Promega). The signal was normalized to the amount of protein in the sample as determined by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2019Quote: ... SDC concentration was adjusted to 1% and 120 µg of proteins from each bacterial culture were digested by addition of trypsin (Promega) in a 1:50 (enzyme:protein ...
-
bioRxiv - Physiology 2019Quote: ... The concentrated urea was diluted 12 times with 50 mM ammonium bicarbonate buffer and proteins were digested for 18 hours at 36°C using trypsin/LysC mixture (Promega) in 1:50 (w/w ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cysteines were then alkylated with 0.5 M iodoacetamide in 100 mM NH4HCO3 (45 minutes in a dark room at room temperature) and proteins were digested overnight with 10 ng/μl trypsin (CAT NO V5280, Promega) in 25 mM NH4HCO3 77 ...
-
bioRxiv - Biochemistry 2019Quote: ... Cells between passage 10 – 30 grown in 40-mm wells were transiently transfected at 30 – 50% confluency with plasmids encoding for TRPM2DR and green fluorescent protein (GFP) using FuGene6 (Promega). Transfected cells were subcultured onto laminin (Sigma-Aldrich ...