Labshake search
Citations for Promega :
1001 - 1050 of 1438 citations for High mobility group protein B1 HMGB1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... and proteins were expressed with a TNT Quick Coupled Transcription/Translation System (Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2021Quote: Mitochondrial 35S-labeled precursor proteins were synthesized using the TNT SP6-coupled transcription/translation kit (Promega) and plasmid-vectors pSP64 containing the genes of interest ...
-
bioRxiv - Biophysics 2021Quote: Analysis of G-protein recruitment was performed by using a modified protocol of NanoBiT system (Promega) assay described previously5 ...
-
bioRxiv - Cell Biology 2022Quote: ... 5 mM iodoacetamide (IAA) and proteins were eluted by digestion with 5 µg/ml trypsin (Promega). Eluted proteins were fully digested overnight ...
-
bioRxiv - Neuroscience 2019Quote: ... 100 µg of protein was then added to 100 µl of prewashed HaloLink resin (Promega G1914), which was prepared by washing and pelleting for 2m at 800xg 3x in wash buffer (100 mM Tris pH 7.5 ...
-
bioRxiv - Molecular Biology 2019Quote: Dried protein samples were digested in 200 mM EPPS buffer pH 8.5 with trypsin (Promega #V5111). Digests contained 2% acetonitrile (v/v ...
-
Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... 30 μg of protein was taken for digestion with trypsin (2% (w/w)) (Promega, United States) and left overnight at 37°C (∼18-hours) ...
-
bioRxiv - Developmental Biology 2021Quote: Proteins were synthesized with the TnT SP6 Quick Coupled Transcription/Translation System (Promega, Madison, Wisconsin, USA).
-
bioRxiv - Microbiology 2019Quote: ... The gene that encodes the HaloTag protein was optimized for Magnetospirillum codons with permission from Promega Corporation (Madison ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins were subsequently digested off the beads by addition of 0.4 µg Trypsin/LysC (Promega, #V5072) for 1 h while shaking in a thermoshaker at 37°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... proteins were digested for 6 h at 37°C with 4ng.μL−1 of modified trypsin (Promega) dissolved in 50 mM NH4CO3 ...
-
bioRxiv - Cell Biology 2019Quote: ... the bead-bound protein was incubated with either 6.7 µM HaloTag-AlexaFluor660 or HaloTag-TMR (Promega), or SNAP-Surface Alex Fluor 647 (NEB) ...
-
bioRxiv - Neuroscience 2019Quote: ... Proteins were digested overnight at 37°C with 1 μg of mass spectrometry grade Trypsin (Promega). The resulting peptide samples were cleaned up for mass spectrometry in a Sep-Pak C18 column (Waters ...
-
bioRxiv - Physiology 2019Quote: ... SDS was removed by chloroform/methanol precipitation and the proteins were proteolytically digested with trypsin (Promega) and purified using OMIX C18 Mini-Bed tips (Agilent Technologies ...
-
bioRxiv - Developmental Biology 2020Quote: Tryptic digestion of bead-bound protein was performed in 5% w/w trypsin (Promega, cat. #V5280), 50mM triethylammonium bicarbonate buffer at 37 °C overnight ...
-
bioRxiv - Developmental Biology 2020Quote: Unlabelled in vitro translated protein was produced using rabbit reticulocyte lysates according to manufacturer’s protocol (Promega).
-
bioRxiv - Biochemistry 2022Quote: ... The protein from lysed cells were digested in 1 µL 10ng/µL of trypsin/LysC (Promega) in 100mM TEAB (ProtiFi) ...
-
bioRxiv - Biochemistry 2022Quote: ... aliquots of EPO proteins were digested respectively using a combination of trypsin and Glu-C (Promega), or Glu-C (Promega) ...
-
bioRxiv - Biochemistry 2022Quote: ... aliquots of EPO proteins were digested respectively using a combination of trypsin and Glu-C (Promega), or Glu-C (Promega) ...
-
bioRxiv - Developmental Biology 2022Quote: ... On-pellet protein digestion was performed in 50 mM ABC (pH 8) by adding TrypLysC (Promega) at an enzyme ...
-
bioRxiv - Microbiology 2022Quote: ... FLAG-tagged or HA-tagged protein was detected using a commercially available mouse monoclonal antibody (Promega).
-
bioRxiv - Molecular Biology 2022Quote: ... were incubated with 35S labeled proteins made by using TNT Quick Coupled transcription/Translation System (Promega) with 35S labeled methionine ...
-
bioRxiv - Systems Biology 2022Quote: ... In-solution digestions were performed on lysates containing 5 mg of protein using either Trypsin (Promega), Chymotrypsin (Promega) ...
-
bioRxiv - Molecular Biology 2023Quote: ... proteins were digested by 0.5 µg lysyl endopeptidase (LysC) (Fujifilm-Wako) and 0.5 µg Trypsin (Promega) at room temperature overnight ...
-
bioRxiv - Microbiology 2022Quote: ... Protein was then digested with 0.5 µg Sequencing Grade Modified Trypsin (V5111 - Promega, Madison, Wisconsin, USA). Following the overnight digestion at 37°C ...
-
bioRxiv - Plant Biology 2023Quote: ... proteins were alkylated with iodoacetamide and digested with Trypsin/Lys-C Mix (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2022Quote: ... The proteins were diluted to 0.1 M ammonium bicarbonate and digested overnight with Trypsin (Promega, V5113) at 37°C with a protein-to-enzyme ratio of 50:1 ...
-
bioRxiv - Cancer Biology 2024Quote: ... the fusion proteins were expressed using the cell-free transcription and translation system (Cat. # L4140, Promega) following the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein samples (15 µg) were digested overnight at 37 °C with trypsin (Promega, Madison, WI-USA) in a protein:trypsin ratio of 50:1 (w/w) ...
-
bioRxiv - Biophysics 2023Quote: ... the proteins were digested into tryptic peptides by incubation with 1□µg sequencing grade trypsin (Promega) overnight at 37□°C ...
-
bioRxiv - Biochemistry 2023Quote: ... and proteins were digested overnight with LysC (FUJIFILM Wako) and trypsin (sequence grade; Promega, Madison, WI) at the protein:enzyme ratio of 100:1 for each enzyme at 25 °C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein aggregation was initiated by addition of Factor Xa protease (SKU PR-V5581, Promega, Madison, WI) at 22◦C ...
-
bioRxiv - Neuroscience 2023Quote: ... Syp-Halo protein was incubated with 5 times molar excess halo ligand Alexa 488 (Promega, USA) overnight at 4°C ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Protein was quantified using the Nano-Glo® HiBiT Extracellular Detection System (N2420, Promega, Madison, WI) using the HiBiT Control Protein (20 µM ...
-
bioRxiv - Neuroscience 2023Quote: ... After the sequential digestion with LysC and trypsin (Promega, each protease to protein ratio 1:80), beads were retained with a magnetic rack and samples were filtered with Costar Spin-X spin filters (0·22 µm cut-off ...
-
Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: Proteins were digested by incubating each gel slice with 10 ng/µl of trypsin (V5111, Promega) in 40 mM NH4HCO3 ...
-
bioRxiv - Systems Biology 2024Quote: ... proteins were digested overnight at 37 °C and 800 rpm using sequencing-grade modified trypsin (Promega) at a protein-enzyme-ratio of 50:1 ...
-
bioRxiv - Cell Biology 2023Quote: ... 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega). Translation reaction products from 0.25 μg of plasmid DNA were incubated with GST-labeled proteins on glutathione-Sepharose beads in NETN-E buffer (50 mM Tris ...
-
bioRxiv - Biochemistry 2023Quote: ... 10 µg of reconstituted protein was digested in 0.05 N HCl with 1 µg pepsin (Promega) without prior reduction/alkylation at a volume of 200 µl 50 °C for 3 h ...
-
bioRxiv - Microbiology 2023Quote: ... the pcDNA3.1+ expression plasmids for spike proteins were co-transfected using Fugene (Promega, Madison, WI, USA) into HEK293T/17 cells ...
-
bioRxiv - Systems Biology 2024Quote: ... pH 8.0 were added and proteins were digested with 1.5 μl trypsin (0.5 μg/μl; Promega) with constant shaking at 1,100 rpm and incubation at 37 °C on a ThermoMixer C incubator for 4 h ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... LgBiT protein either purified in-house (His-LgBiT) or purchased from Promega (N401A, 200 µM stock) was diluted to 20 pM in PBS pH 7.4 solution and dispensed into white 96 well non-binding surface plate (Corning #3600 ...
-
bioRxiv - Immunology 2024Quote: ... PKA activation in H9c2 cells was measured using SignaTECT cAMP-dependent protein kinase assay system (Promega) according to the manufacturer’s instruction ...
-
bioRxiv - Biophysics 2024Quote: ... The purified fused protein was cleaved by treating it with Factor Xa (FXa; Promega, Madison, WI) at room temperature ...
-
bioRxiv - Biochemistry 2024Quote: ... Proteins were digested with Lys-C (Wako Chemicals) and trypsin (1:40 ratio, Trypsin Gold, Promega). After complete digestion had been confirmed by nanoLC-MS/MS (<7% missed cleavage sites) ...
-
bioRxiv - Cell Biology 2024Quote: ... Protein lysates from the single cells were digested with 2 ng of Trypsin (Promega, Cat. V5280), dissolved in 1 μl of 100 mM TEAB pH 8.5 containing Benzonase (Sigma ...
-
Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... 24h prior to imaging cells were co-transfected with human PEX31-42-GFP-Halo and either human HAP1-mCherry-eDHFR or mouse BICD21-572-mCherry-eDHFR using FuGENE 6 (Promega; 1 µg total DNA) and 48h prior to imaging with control siRNA using Lipofectamine RNAiMAX (Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Standard curves for each primer set were generated using serially diluted plasmid (for gAAV) or Human and Mouse Genomic DNA (for host) (Promega G1521 and G3091, respectively) and used for quantification ...
-
bioRxiv - Biochemistry 2024Quote: The upstream promoter region of the human ORAI1 gene with the putative G4-forming sequence ORAI1-Pu was amplified by PCR using human genomic DNA as a template and then cloned into the pGL4.72[hRlucCP] vector (Promega; Madison, USA; catalog no. E6901) at the KpnI/HindIII site ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were renatured and assayed according to the Nano-Glo® In-Gel Detection System protocol (Promega). Light produced by NLuc was captured using a Chemidoc MP Imager (Biorad).