Labshake search
Citations for Promega :
901 - 950 of 2516 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA quality and concentration were estimated via PicoGreen (Promega). Genome library preparations took place at the Broad Institute using a Nextera XT Library Preparation Kit (Illumina) ...
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bioRxiv - Cancer Biology 2021Quote: Genomic DNA from cell lines was isolated using Maxwell® 16 Instrument and the Maxwell® 16 DNA Purification Kit (Promega Cat.# AS1020) following the protocol provided ...
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bioRxiv - Genomics 2019Quote: Genomic DNA was isolated from 30 ul of chicken blood samples using the Promega Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... genomic DNA from puromycin selected cells and wild-type HEK293 cells was extracted (Promega Wizard Genomic DNA Purification Kit #A1120; Promega, Madison, WI, USA) for PCR amplification of a 535 bp region flanking the sgRNA cleavage site ...
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bioRxiv - Microbiology 2020Quote: ... for 20 min at 56°C before DNA was purified with Maxwell® RSC Blood DNA Kit (Promega Pte. Ltd., Cat. No. AS1400). DNA concentration was quantified using Qubit® 2.0 fluorometer ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA was extracted from leaf tissue using a modified protocol for Wizard(R) Magnetic 96 DNA Plant System from Promega (Madison, Wisconsin, USA). The copy number of integrated T-DNA in transgenic soybean plants was measured in duplex TaqMan assays ...
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bioRxiv - Plant Biology 2022Quote: Healthy young leaves from each line were collected and genomic DNA was extracted using a Maxwell RSC Plant DNA Kit (Promega, Madison, WI, USA). The presence or absence of the 5.46-kb fragment containing DAI was confirmed by PCR (primers 1 and 2) ...
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bioRxiv - Microbiology 2021Quote: ... A 2 ml aliquot of the overnight enrichment was centrifuged at 6000 RPM for 10 minutes and then DNA was extracted using the Maxwell RSC Cultured Cells DNA Kit (Promega, Madison, WI, USA). Extraction was performed according to manufacturer’s recommendations with the addition of RNase A after lysis ...
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bioRxiv - Microbiology 2020Quote: ... Single-cell clones were obtained and the genomic DNA was then isolated using a Wizard SV genomic DNA purification kit (Promega, Madison, WI, USA). The target gene was amplified by PCR using pMXs vector forward (5’GGTGGACCATCCTCTAGA3’ ...
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bioRxiv - Microbiology 2021Quote: ... DNA was eluted with 330 µl 1x TE buffer and 100 µl of DNA samples were pipetted into a 96-well microtiter plate where DNA concentration was determined using QuantiFluor® dsDNA System (Promega Corporation, ZDA) according to the manual ...
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bioRxiv - Microbiology 2020Quote: Samples from the human subject were extracted with a DNA extraction protocol adapted from the Maxwell RSC Buccal Swab DNA kit (Catalogue number AS1640: Promega Corporation, Madison WI). Briefly ...
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bioRxiv - Microbiology 2022Quote: 293A cells were seeded into 20-mm wells of 12-well cluster dishes with or without glass coverslips and the next day transfected with 500 ng DNA mixes/well containing expression vectors (250 ng) and pUC19 filler DNA (250 ng) using Fugene HD (Promega, Madison, WI, USA) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... an inhibition test was performed by mixing the soil DNA extracts with a control plasmid DNA (pGEM-T Easy Vector, Promega, Madison, WI, USA) or water ...
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bioRxiv - Microbiology 2024Quote: ... Six presumptive transconjugant colonies were randomly selected and grown overnight in 1 mL of LB prior to DNA extraction using the Wizard® Genomic DNA Purification kit (Promega, Madison, WI). The AMR genotypes of transconjugant colonies were determined by polymerase chain reaction (PCR ...
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bioRxiv - Microbiology 2023Quote: DNA from 1 ml of harvested phage stock was extracted using Wizard PCR DNA Purification Resin and Minicolumns (both Promega, Madison, WI, USA) as described previously [49] ...
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bioRxiv - Evolutionary Biology 2023Quote: ... senegalensis DNA was extracted from muscle tissues in thoraxes using Maxwell® 16 LEV Plant DNA Kit (Cat. No. AS1420, Promega, WI, USA). Details on extraction and library preparation protocols are provided in the Supporting Text 1.
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bioRxiv - Genomics 2024Quote: ... Both obtained telomeric DNA and 18S ribosomal DNA (rDNA) were then purified by Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) and quantified by Qubit 3.0 fluorometer.
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bioRxiv - Synthetic Biology 2024Quote: Genomic DNA was isolated from plant cells using the Promega Wizard® SV 96 Genomic DNA Purification System (Promega Corporation, Madison, WI, USA) following the manufacturer’s protocol using the Tecan Fluent (Supplementary Figure S2E).
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: HEK293 cells growing at 70% confluency in a 10-cm dish were transfected with wild-type human NOPR or NOPLight (3 μg DNA) and GloSensor-20F (Promega, 2.5 μg DNA) using 12 μL Lipofectamine 2000 (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Individual genotyping at every generation was performed via PCR on genomic DNA extracted from larvae pseudopods (Wizard® Genomic DNA Purification Kit, Promega, Madison, WI, USA) using gene-specific primers (forward ...
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bioRxiv - Microbiology 2022Quote: ... The resulting suspension was then centrifuged at 14,000 rpm at 4°C to remove cellular fragments and the genomic DNA finally was extracted using the Wizard® Genomic DNA Purification Kit reference A1125 (Promega, Madison, WI, USA) following the bulla guidelines ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from each sample taken from captured birds and carcasses using the Wizard Genomic DNA Purification kit (Promega Corp., Madison, Wisconsin, USA) according to the manufacturer’s instructions and eluted in 125 µl of DEPC-treated water ...
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bioRxiv - Microbiology 2022Quote: ... Primers flanking the upstream and downstream regions of each gene of interest were amplified from PA14 genomic DNA (Promega Wizard Genomic DNA Purification Kit) and extracted with GeneJet Gel Extraction Kit (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... Colonies were collected and subjected to total DNA extraction using the Maxwell® 16 Tissue DNA Purification Kit in the Maxwell 16 Research Instrument (Promega, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA from the bacterial strains used as positive controls was extracted using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, U.S.) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA and RNA were extracted from cell pellets using the Maxwell® RSC Cultured Cells DNA and RNA Kit (Promega, AS1620 and AS1390, respectively) with the Maxwell® RSC 48 Instrument (Promega ...
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bioRxiv - Genomics 2023Quote: ... DNA extraction from pre-enrichments and selective enrichments was done using the Promega Maxwell® RSC cultured cells DNA kit (Promega, WI, USA, AS1620) according to the manufacturer’s specifications on the Promega Maxwell® RSC 48 Instrument ...
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bioRxiv - Immunology 2021Quote: ... and cleaned from excess DNA with DNAse 1 enzyme (Promega). RNA samples were reverse transcribed to cDNA and an anchor sequence at the variable part of the TCR was added using single strand ligation ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The reaction contained 1 unit of GoTaq DNA polymerase (Promega), 1X enzyme Flexi Buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... DNA fragments were cloned into pGEM-T Easy Vector (Promega). DNA templates for in vitro transcription were amplified using M13F and M13R primers ...
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bioRxiv - Microbiology 2020Quote: ... Contaminating genomic DNA was depleted by two DNase treatments (Promega), according to manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2021Quote: PCR (RT-PCR) was conducted using GoTaq DNA polymerase (Promega). PCR programs were the following ...
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bioRxiv - Developmental Biology 2021Quote: ... and spiked-in with 0.3% of unmethylated Lambda DNA (Promega). Bisulfite conversion was performed using EZ DNA Methylation-Gold Kit (Zymo Research ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA quantification was performed using the Quantifluor dsDNA system (Promega). The samples were pooled in a single library ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.02ng of unmethylated lambda phage DNA (Promega, Madison, WI, USA) and 0.0001ng of pUC19 plasmid methylated at 100% of CpG sites (New England BioLabs ...
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bioRxiv - Genomics 2020Quote: ... Extracted DNA was quantified with the Quantus dsDNA system (Promega) and quality was assessed by FEMTOpulse (Agilent) ...
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bioRxiv - Immunology 2021Quote: ... Ligation was performed using T4 DNA Ligase (Promega; Cat.# M1801) using manufacturer’s protocol for staggered end ligation ...
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bioRxiv - Molecular Biology 2021Quote: The CD19 minigene was amplified from human genomic DNA (Promega) with the primers 5’-catAAGCTTgaccaccgccttcctctctg-3’ and 5’-catGAATTCNNNNNNNNNNNNNNNGGATCCttcccggcatctccccagtc-3’ ...
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bioRxiv - Microbiology 2019Quote: ... DNA concentration was measured using the Quantus fluorimeter (Promega, USA).
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bioRxiv - Genetics 2019Quote: ... 0.2 µl of GoTaq® DNA Polymerase (Promega, Madison, WI), 5 µl of GoTaq® Reaction Buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR was performed using GoTaq G2 DNA Polymerase (Promega, USA) according to manufacturer’s instructions.
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bioRxiv - Pathology 2019Quote: ... 0.5 U GoTaq™ DNA Polymerase (Promega Corp., Madison, Wisconsin), 1× Colorless GoTaq™ PCR Buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... ~80 % confluent cells were transfected with DNA: FuGENE HD (Promega) ratio of 1:3 for all transient transfection presented ...
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bioRxiv - Microbiology 2019Quote: ... DNA concentration was measured using the Quantus fluorimeter (Promega, USA).
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bioRxiv - Biophysics 2019Quote: ... expression plasmids were supplemented with empty vector DNA (pSI; Promega) to maintain the same ZP subunit/total DNA ratio.
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bioRxiv - Microbiology 2019Quote: ... The Wizard genome DNA purification kit (Promega Corp., Madison, WI) was used for isolation of bacterial genomic DNA (gDNA) ...
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bioRxiv - Genomics 2019Quote: ... the DNA quality was analyzed using QuantiFluor dsDNA system (Promega). Nextera XT kit (Illumina ...
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bioRxiv - Genomics 2019Quote: ... spiked with 0.5% unmethylated Lambda DNA (Promega, Madison, WI, USA), was sheared to 250 bp fragments with a Covaris S2 sonicator (Covaris) ...
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bioRxiv - Genetics 2020Quote: ... The PCR is carried using GoTaq DNA polymerase (Promega, UK) and the following PCR conditions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 U/μl GoTaq Flexi DNA Polymerase (Promega, WI, USA), and 1 μl of DNA template ...