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Citations for Promega :
851 - 900 of 1637 citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... the C terminus of MC1R was fused with a 15-amino-acid polypeptide linker (GSSGGGGSGGGGSSG) and a LgBiT (Promega).
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bioRxiv - Molecular Biology 2021Quote: ... Digestion was performed for 8 h at 37°C using 70 ng modified porcine trypsin (Promega, Fitchburg, WI, USA). Tryptic peptides were extracted using 70% acetonitrile and dried using a SpeedVac vacuum concentrator.
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bioRxiv - Neuroscience 2021Quote: ... all DNA constructs were prepared from bacterial cultures grown at 37 °C using PureYield™ Plasmid Midiprep System (Promega) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Samples were digested initially with LysC (WAKO) at 37°C for 2.5 h and subsequently with 10 μg trypsin (modified sequencing grade, Promega) overnight ...
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bioRxiv - Microbiology 2020Quote: ... was then diluted into fresh 50mM ammonium bicarbonate and incubated overnight at 37°C either with 0.056 μg of trypsin (Promega), chymotrypsin (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Alkylation was quenched by the addition of 3 mM DTT and proteins were digested overnight at 37°C with 1 μg trypsin (0.5 μg/μl; Promega). Following digestion ...
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bioRxiv - Genomics 2021Quote: ... ligation was performed at 15°C for 16 hours using 100 ng of the digested DNAs and 4.5 U of T4 DNA ligase (Promega) in a final volume of 100 μl ...
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bioRxiv - Cancer Biology 2022Quote: ... Precipitates were solubilised in 8M urea (20mM HEPES, pH 8) and digested with Lysine C (1:50 ratio; Promega) for 4h at 37C in 6M urea followed by an overnight trypsin digestion (1:25 ratio ...
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bioRxiv - Molecular Biology 2022Quote: ... The human Gβ1 with a C-terminal 15-amino acid polypeptide linker was followed by a HiBiT (peptide 86, Promega), and the scFv16 was modified with an N-terminal GP67 signaling peptide and a C-terminal 8× histidine tag ...
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bioRxiv - Molecular Biology 2023Quote: ... A second digestion was performed o/n at 37°C while shaking at 1,400 rpm using 500 ng of sequencing grade modified trypsin (Promega). Trypsin digested peptides were separated from Ni-NTA beads by filtering through a 0.45 μm filter Ultrafree-MC-HV spin column (Merck-Millipore).
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bioRxiv - Developmental Biology 2024Quote: ... Washes were performed 6x with 200 µL each and digestion was performed with 0.75 µg Trypsin/Lys-C Mix (Promega) per 10 µg total protein ...
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bioRxiv - Microbiology 2024Quote: ... Poly-C-tails were added to 1 µg of each end-repaired sample in duplicate using Terminal Transferase (Promega) with a ratio of 9.5 mM dCTP to 0.5 mM ddCTP to limit chain length and DNA Duplicate reactions were combined and purified with SPRIselect beads ...
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bioRxiv - Systems Biology 2023Quote: ... cooled on ice to room temperature for 5 min and digested overnight at 37 °C with 0.5 μg of sequencing-grade trypsin (Promega). Peptide mixtures were acidified to pH 3 with 1.5 µl 5% FA ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated for 30 min at 4 °C slowing rotating with Passive Lysis Buffer (Promega Cat#E1941). After incubation with the lysis buffers ...
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bioRxiv - Molecular Biology 2023Quote: ... Full-length GID4 N or C-terminal NanoLuc-fusion was transfected into HEK293T cells using FuGENE HD (Promega, E2312) according to manufacturer’s instructions and proteins were allowed to express for 20 hours ...
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bioRxiv - Microbiology 2022Quote: ... Samples were digested for 2 hr at 25°C with constant agitation with a Trypsin/LysC mix (V5071, Promega), and overnight at 25°C with proteomics-grade porcine trypsin (T6567 ...
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bioRxiv - Systems Biology 2023Quote: ... Digestion was performed overnight in 300 μL of 50 mM 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES) buffer at pH 7.5 supplemented with endoproteinase Lys-C (Wako) and trypsin (Promega) at room temperature ...
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bioRxiv - Pathology 2023Quote: ... Overnight digestion was performed at 37 °C with 2 μL of freshly prepared trypsin at 0.1 μg/μl (V5111, Promega) in 50 mM ammonium bicarbonate (per 100 μl of sample) ...
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bioRxiv - Plant Biology 2023Quote: ... Sample C was treated with 1% Triton X-100 (TX100) followed by treatment with 1μg mL-1 trypsin (Promega). For the detergent treatment ...
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bioRxiv - Systems Biology 2023Quote: ... Plates were incubated at 37°C for 96 hours and lysed using CellTiter-Glo Reagent (Promega, catalog. no. G7570). Luminescence was measured using a PHERAstar FS instrument and growth inhibition was calculated relative to DMSO-treated wells.
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bioRxiv - Biochemistry 2023Quote: ... samples were washed twice with S-Trap buffer before overnight digestion at 37° C with Trypsin (Promega Cat# V511X) in digestion buffer (50 mM ammonium carbonate ...
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bioRxiv - Cell Biology 2023Quote: DNA was prepared from bacterial cultures grown at 37°C using a Midiprep kit (PureYield Plasmid Midiprep System, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... at 10,000 x g and 4°C for 2 min and digested by addition of RNAse-free DNAse I (Promega) for 10 min on ice ...
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bioRxiv - Biochemistry 2023Quote: ... each sample was digested overnight at 37 °C with one or more of the following enzymes separately: trypsin (Promega), Lys-C (Roche) ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were initially digested for 1 hour at 47°C with sequencing grade trypsin (Promega, San Luis Obispo, CA) dissolved in 50 mM TEAB (pH 7 ...
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bioRxiv - Genetics 2023Quote: ... proteins were extracted by chloroform-methanol precipitation and digested with 2 μg trypsin/Lys-C mixture (Promega, Madison, WI). Samples were labeled with 10-plex tandem mass tag reagents (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Beads were resuspended in 500 µL of 100 mM Tris-HCl pH 8.0 containing 2 mM CaCl2 and digested at 37 °C for 1 h with 0.2 μg of trypsin/LysC (#V5073 Promega). The samples were then loaded onto homemade SepPak C18 Tips packed by stacking three AttractSPE disk (#SPE-Disks-Bio-C18–100.47.20 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Probe synthesis reactions were performed at 37 °C for 3 h and then were treated with DNase I (Promega) at 37 °C for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... Plates were then incubated at 37°C overnight before luciferase activity was quantified using the Luciferase Assay System (Promega) on SoftMax Pro v.7.0.2 (Molecular Devices) ...
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bioRxiv - Microbiology 2023Quote: ... OD600nm was monitored every hour at 37°C for 24 h by using a GloMax Explorer plate-reader (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... beads were reconstituted in 5 μL 50 mM HEPES pH 8.0 buffer containing trypsin/rLys-C enzyme mix (Promega) at a 1:25 enzyme to protein ratio ...
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bioRxiv - Cell Biology 2024Quote: ... we isolated individual clones and screened for expression using the C-terminal nanoluc fusion (Nanoluc Lytic Detection Kit, Promega), followed by additional characterization of SMO agonist-induced ciliary accumulation via microscopy ...
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bioRxiv - Cell Biology 2024Quote: ... reduced (6.5mM DTT, 1 hour at 60°C), alkylated (54mM Iodoacetamide, 30min, RT) and digested with 1µg of Trypsin (Promega) (96) ...
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bioRxiv - Genomics 2024Quote: ... The column was washed twice with 150 µL of loading buffer and gently centrifuged before overnight digestion at 37°C with 0.1 µg/µL of trypsin (Promega) in 50 mM ammonium bicarbonate ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were then divided in two to obtain tryptic and Glu-C peptides and diluted to final concentration of 0.1 mg/mL with 50 mM NH4HCO3 for Trypsin digestion and with PBS for Glu-C (Promega) digestion ...
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bioRxiv - Biochemistry 2024Quote: ... The translation mixtures were incubated at 30°C for 90 mins followed by the dual-luciferase reporter assay (Promega). The 2μl translation products were added into a white 384-well microplate (PerkinElmer) ...
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bioRxiv - Cancer Biology 2024Quote: Purified rRNA (1 to 3 µg) was digested overnight at 37 °C with 270 units of Nuclease S1 (Promega) using the supplied buffer ...
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bioRxiv - Biophysics 2020Quote: ... of the different TALE-TF using staining with 1.25 μM HaloTag-TMR ligand following the protocol of the manufacturer (Promega).
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bioRxiv - Biophysics 2020Quote: ... we stained the cells directly before the measurement with 1.25 μM Halo-TMR-ligand according to the protocol of the manufacturer (Promega). We supplemented the medium for labelling and imaging with 200 μM Auxinole (Aobious ...
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bioRxiv - Biophysics 2022Quote: ... eGFP was purified and labeled with halotag Succinimidyl Ester (O4) ligand as previously described (Huang et al., 2017) (Promega).
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bioRxiv - Biophysics 2019Quote: ... the fluid chambers were exposed to a PBS pH 7.4 solution containing 1% glutaraldehyde to subsequently immobilize the reference beads (Spherotec) and the HaloTag ligand (Promega).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... NanoBRET™ Nano-Glo® Substrate - furimazine (#N1663) and HaloTag® NanoBRET™ 618 Ligand (#G9801) were from Promega. Dimethylsulfoxide (DMSO ...
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bioRxiv - Microbiology 2023Quote: ... 300 µl of eluant (at 1.5 mg/ml) was treated with 6.5 µl of 1 mM HaloTag Alexa Fluor 488 Ligand (Promega PAG1001). Both labeled and unlabeled proteins were subject to size exclusion chromatography for further purification using Superose 6 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... penicillin 100 U/ml and streptomycin 100 μg/ml) +/-HaloTag® NanoBRET™ 618 Ligand (0.5 μl/ml, Promega). 4 h later ...
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bioRxiv - Bioengineering 2023Quote: HaloTagged AP-2 was visualized in the SUM159 cells through the addition of membrane-permeable JaneliaFluor646-HaloTag ligand (Promega) at a concentration of 125 nM ...
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bioRxiv - Cell Biology 2023Quote: ... 4 hours post-transfection media was replaced with fresh complete DMEM containing JF646 HaloTag ligand (20 nM; GA112A; Promega) when required ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Target engagement and intracellular availability for VHL ligands were assessed using the NanoBRET TE Intracellular E3 ligase assay (Promega). HEK293 cells were transfected with the VHL-NanoLuc fusion constructs using FuGENE HD (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 4% FBS and seeded in 96-well plates at a density of 3.5×105 cells/mL in the presence or absence of 0.1 mM HaloTag NanoBRET™ ligand (Promega). Following overnight incubation ...
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bioRxiv - Developmental Biology 2023Quote: ... HEK293 culture medium was replaced by imaging medium containing either Janelia Fluor 646 (JF646) ligand (10 nM; Promega, #GA1120) or OregonGreen ligand (50 nM ...
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bioRxiv - Biophysics 2024Quote: ... we stained the cells with 1 ml of a 1:2,000 TMR Halo-ligand solution (Promega, Cat. No.: G8251) to fluorescently label the Halo-tagged proteins ...