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Citations for Promega :
851 - 900 of 4906 citations for 7 8 Dimethoxy 2 3 4 5 tetrahydro 1H benzo e 1 4 diazepine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Plasmid DNA was combined at a 3:1 ratio of FuGENE® 6 Transfection Reagent (Promega) to DNA and incubated 20 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... the cells were co-transfected using Fugene HD transfecting reagent at a 1:3 ratio (Promega) along with pRL-TK Renilla (Renilla Luciferase ...
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bioRxiv - Plant Biology 2023Quote: ... The PHN-SpCas9-4×BsaI was then digested with BsaI and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). The annealed oligos FaPDSF1/R1 and FaPDSF2/R2 were mixed and ligated to the purified pHN-SpCas9-4×BsaI to form the final pHN-SpCAS9-FaPDSsgRNAs construct.
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bioRxiv - Cancer Biology 2021Quote: ... 50μl of either caspase-8 reagent or caspase-9 (Promega) reagent was then added with MG132 proteosome inhibitor ...
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bioRxiv - Biochemistry 2022Quote: ... 50 mM Tris pH 8 overnight at 37 °C (Promega) at an estimated protein:enzyme ratio 1:100 ...
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bioRxiv - Biochemistry 2021Quote: ... treated with 3 mM dithiothreitol (DTT) (molecular grade, Cat. 3483-12-3, Promega), and incubated for 30 min at 37°C in 5% CO2 in serum-free RPMI media ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was diluted either 1:5 or 1:10 for all qPCR experiments and GoTaq qPCR Master Mix (Promega) was used to amplify cDNA ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay Virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system (RNA1 qPCR primers GW194 and GW195)23.
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bioRxiv - Biochemistry 2023Quote: ... All samples were then diluted 8-fold with 100 mM NH4HCO3 and 1 mM CaCl2 and digested with sequencing-grade modified porcine trypsin (Promega, WI, USA) provided at a 1 ...
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bioRxiv - Microbiology 2022Quote: Various KSHV mRNA 5’-UTRs containing uORFs were cloned up stream of the Renilla luciferase gene in a psiCHECK™-2 vector (Promega) using a Gibson Assembly® Cloning Kit (New England Biolabs) ...
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bioRxiv - Microbiology 2019Quote: ... 5 μl of 5× Gotaq buffer (Promega M792A), 1μl of 10μM forward primer ...
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bioRxiv - Plant Biology 2021Quote: The optimised HaloTag®-7 sequence (298 amino acids) from Promega (https://www.promega.de/) was genetically split on position 155/156 aa into the N-terminal fragment “NHalo” (aa 1-155 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cleaved caspase 7 was measured using a Caspase Glo assay (Promega, Madison, WI) as described by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... 7 and 10 were either cloned with the pGEM®-T kit (Promega) to assay individual sequences and/or sequenced by Illumina (Centre d’expertises et de services Genome Québec) ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were rinsed 5 times for 8 min in PBS-tween 0.2% and incubated with secondary antibodies for 2 h at room temperature under agitation (HRP anti-mouse, 1:10000, Promega cat. # W4021). Membranes were further rinsed 5 times for 8 min in PBS-tween 0.2% ...
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bioRxiv - Evolutionary Biology 2024Quote: ... for 30 minutes then with 5 units/1 L RQ1 RNase-free DNase (Promega) for 10 minutes at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... 5 x 106 cells were electroporated with 1 μg of NanoLuc pNL3.1 (Promega, N1031) vector and 0.2 μg of control vector ...
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bioRxiv - Developmental Biology 2021Quote: ... abdomens were washed with phosphate-buffered saline with 0.1% Tween (PBST) four times for 15 minutes before RNase (Promega, original concentration of 4 mg/mL) was added in a concentration of 10 μL/mL for 20 minutes ...
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bioRxiv - Genetics 2021Quote: Senescence was measured using 2 assays: 1/ Beta-Glo Assay kit (Promega # E4720), according to the manufacturer’s instructions and utilizing a luciferin-galactoside substrate (6-O-β galactopyranosylluciferin) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transiently transfected with 1 or 2 µg HaloTag-Ubiquitin (N2721, Promega) or the control vector pHTN HaloTag CMV-neo (G7721 ...
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
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bioRxiv - Genomics 2021Quote: ... cells were transfected in a 3:1 ratio of FuGENE® HD Transfection Reagent (Promega cat # E2311) and plasmid DNA (pcDNA3.1-HMGB1P1-3xHA ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 μg of purified RNA was incubated with 3 units of RQ1 DNase (Promega) at 37°C for 30 mins ...
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bioRxiv - Molecular Biology 2021Quote: ... protein digestion was performed by 1 µg of Lys-C (Wako) at 37 °C for 3 h following 1 µg of trypsin (Promega, Madison, WI, USA) at 37 °C for 16 h ...
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bioRxiv - Immunology 2023Quote: ... samples were diluted 1:1 with water and digested for 1.5 hours at 37 °C with 1 µg of LysC and overnight at 37 °C with 1 µg trypsin (Promega). The peptide mixture was acidified with trifluoroacetic acid (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... UDP (at concentrations ranging from 1 nM to 1 mM) was then applied with a final concentration of 5 μM furimazine (NanoLuc Luciferase Assay, Promega). Luminescence was subsequently measured using a Victor X5 multi-label plate reader (PerkinElmer).
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bioRxiv - Biochemistry 2021Quote: ... Tryptic samples were subsequently diluted to 2M urea in 100mM Tris/HCl pH 8 and digested over night at 37°C (Promega, enzyme:protein 1:100). Samples were adjusted to pH 2 using 10% trifluoroacetic acid (TFA ...
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bioRxiv - Biochemistry 2022Quote: ... 3 units/mL RNasin (Promega). Depending on the experiments (see results) ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized via reverse-transcription of 2-5 μg of RNA using M-MLV Reverse Transcriptase (200 U, Promega, Wisconsin, USA), random primers (10 ng/μl ...
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bioRxiv - Biochemistry 2019Quote: ... The samples were then diluted with 150 μL PBS and to this was added 1 mM CaCl2 (2 μL of a 200 mM stock in water) and trypsin (2 μg, Promega, sequencing grade, V5111) and the digestion was allowed to digest overnight at 37 °C with shaking ...
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bioRxiv - Immunology 2022Quote: ... Caspase-Glo® 8 and Caspase-Glo® 9 (all Promega) kits were used as previously described (57) ...
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bioRxiv - Microbiology 2020Quote: ... 8 hpi cells were lysed in Cell Culture Lysis Reagent (Promega). Lysates were combined with Luciferase Substrate (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... or using Caspase-Glo® 8 Assay Systems® (Promega, G8200) to measure cleaved Caspase 8 activity.
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bioRxiv - Genetics 2024Quote: ... Proteins were immediately solubilized in 8 M urea and tryptic (Promega) digestion was performed overnight at 37°C with shaking followed by desalting ...
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bioRxiv - Molecular Biology 2020Quote: ... 150 mM NaCl, 1 mM MgCl2, 2 mM EDTA, 1% NP-40, 1 mM DTT, 100 U/mL RNasin [Promega], 1X protease inhibitor) at 7 dpi ...
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bioRxiv - Cancer Biology 2022Quote: ... After 7 days of culturing 100 μl of CellTiter-Glo 3D (Promega Cat# G9681) was added to each well and incubated for 5 minutes on a shaker ...
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bioRxiv - Molecular Biology 2019Quote: ... gDNA was isolated after 7 days using Wizard® Genomic DNA Purification Kit (Promega) for preparation of 16S Metagenomic Sequencing Library.
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bioRxiv - Molecular Biology 2021Quote: ... probes listed in Supplementary Table 7 were cloned in pGEM®-T Easy (Promega) for T7 polymerase transcription.
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bioRxiv - Cell Biology 2024Quote: ... biological replicates (n=7) were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described (Hubner et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... terminated by incubation on ice and diluted 1:5 in Glo Lysis Buffer (Promega # E2661). 25 μL of prepared Nano-Glo reagent (Promega # N1120 ...
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bioRxiv - Biochemistry 2023Quote: ... with 25 μL of diluted reactions (diluted 1:5 in Glo Lysis Buffer; Promega # E2661) and incubated at room temperature for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... for Calu-3 cells and CellTiter-Glo 3-D Cell Viability Assay Kit (Promega, Germany) for hBAECs according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... A DENV-1 3’UTR specific probe was generated by PCR reaction with GoTaq Polymerase (Promega, Wisconsin, USA) containing DIG DNA-labelling mix (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were transfected with the Piggybac plasmid plus transposase at a 3:1 ratio using Fugene HD (Promega) and selected with G418 (300 µg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... Elution was conducted in 3 beads volume of proteasome buffer containing 1 μL of TEV protease (Promega, PRV6101) for 1 hr at 37°C.
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bioRxiv - Systems Biology 2024Quote: ... followed by a 3:1 dilution with 100mM ammonium bicarbonate and addition of 2µg sequence-grade trypsin (Promega). Samples were digested at room temperature overnight and acidified with formic acid (final concentration 1%) ...
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bioRxiv - Microbiology 2021Quote: ... diluted 1:2 with PBS and read on a GloMax Multi+ Detection System (Promega). CSV files were exported onto a USB flash drive for analysis.
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bioRxiv - Systems Biology 2020Quote: ... The transfections were performed with 1 μg DNA: 2 μl Fugene HD (Promega E2311) ratio ...
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bioRxiv - Microbiology 2022Quote: ... diluted 1:2 with PBS and read on a GloMax Multi+ Detection System (Promega).