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Citations for Promega :
701 - 750 of 3832 citations for Mouse Alpha ketoglutarate dependent dioxygenase FTO FTO ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... amplicons from three PCR runs for each root sample were combined (Clement et al., 1998) and then purified using a PCR purification kit (PCR Clean-up Kit; PROMEGA Inc., Wisconsin, USA).
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bioRxiv - Neuroscience 2020Quote: ... DNA and RNA were extracted from formalin-fixed paraffin-embedded (FFPE) tissue using the Maxwell 16 FFPE Plus LEV DNA Kit and Maxwell 16 LEV RNA FFPE Purification Kit (Promega, Madison, WI, USA), according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... membrane integrity (CytoTox-OneTM homogeneous membrane integrity assay kit: lactate dehydrogenase (LDH release) and caspase activity/ apoptosis (Caspase-Glo® 3/7 assay system kit) (all from Promega, WI, USA), as described previously17 (see Supplementary data for detailed description).
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times in TBST for 5 minutes and further incubated with HRP-conjugated secondary anti-mouse-IgG-H&L chain (Promega) or anti-rabbit-IgG-F(ab’)2 (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... washed three times (10 minutes each) with PBST before incubation for 1 h with α-Mouse IgG HRP Conjugate (Promega) secondary antibody (1:10 000 in PBST) ...
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bioRxiv - Neuroscience 2020Quote: 1μg of total RNA from the human and mouse brain specimen was reverse-transcribed using the M-MLV reverse transcriptase (Promega) for efficient synthesis of the first-strand cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-mouse HRP conjugate (W4021, 1:10,000) and Anti-Rabbit HRP Conjugate (W4011, 1:10,000) were purchased from Promega (Madison, WI). Anti-COPI antibody was a gift from Charles Barlowe (Dartmouth Univ ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times in TBST for 5 minutes and were then incubated with HRP-conjugated secondary anti-mouse-IgG-H&L chain (Promega) or anti-rabbit-IgG-F(ab’)2 (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: A DNA fragment bracketing the proximal promoter and transcription start site of the Iqsec3 gene (–280 to +36) was PCR-amplified from mouse genomic DNA and subcloned into the pGL3-Basic vector (Promega). Full-length mouse Npas4 cDNA was subcloned in pcDNA3 (Gibco-Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... antibodies before probing with horseradish peroxidase (HRP)-conjugated anti-rabbit (for H3) or anti-mouse (for anti-GFP and anti-TUB) as the secondary antibody (1:5000; Promega, Madison ...
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bioRxiv - Molecular Biology 2021Quote: ... Total genomic DNA extracted from liver mouse and HEK293 cells were used as templates for PCR-cloning of the mouse/human promoter/intron fragments into the KpnI/MluI sites of pGL3 basic luciferase reporter vector (Promega). The glutamic acid (E ...
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bioRxiv - Genomics 2021Quote: ... Membranes were washed three times in TBS-T before incubating with secondary antibodies (HRP-anti Mouse-IgG (H+L) (Promega)) for 45 min in TBS-T and 2% milk ...
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bioRxiv - Cell Biology 2020Quote: ... HRP Conjugate (W4011, 1:5000), and anti-Mouse IgG (H+L), HRP Conjugate (W4021, 1:5000) were purchased from Promega.
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bioRxiv - Neuroscience 2022Quote: DNA constructs with different WT and mutated 5’UTRs of mouse Sox2 mRNA were assembled in the psiCheck-2 vector (Promega) including synthetic Renilla luciferase gene (hRluc ...
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bioRxiv - Cancer Biology 2022Quote: ... The blots were incubated with secondary antibody (horseradish peroxidase-conjugated goat anti-mouse (Cat# W402B) or goat anti-rabbit IgG (W401B) from Promega, Madison ...
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bioRxiv - Microbiology 2020Quote: ... used at a 1:10,000 dilution and the secondary antibody was an Anti-Mouse IgG HRP conjugate antibody (Promega, #W4021) used at a 1:10,000 dilution.
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bioRxiv - Microbiology 2020Quote: ... the plates were washed with PBS-T five times and added with a 1:5000 dilution of HRP-conjugated goat anti-mouse IgG antibody (Promega) in blocking buffer at 37 °C for 45 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Reporter plasmids were constructed by inserting the mouse Olig2 gene mR2 and mR6 regions identified by the ChIP assays in situ into the pGL3-Basic vector (Promega), respectively ...
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bioRxiv - Cell Biology 2021Quote: The luciferase reporter plasmid was generated by cloning the mouse HDAC7 promoter into the NheI site of pGL4 Luciferase reporter plasmid (Promega). The mouse HDAC7 promoter was amplified using Platinum SuperFi II DNA Polymerase (12361010 ...
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bioRxiv - Immunology 2020Quote: ... were used as primary antibodies and horseradish peroxidase (HRP)-conjugated secondary antibodies were from Promega (Rabbit W401B and Mouse W402B), and the substrate ECL was detected by Pierce ECL2 solution (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: OCA2 enhancer region was cloned from mouse genomic DNA into KpnI and XhoI sites of the pGL3 promoter vector (E1751, Promega). MITF proximal promoter was cloned into SacI and HindIII sites of the PGL4 promoter vector (E6651 ...
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bioRxiv - Molecular Biology 2020Quote: ... we isolated the sequence-paired sites from the native mouse and human Hes1 and Hes5 genes and cloned these fragments into the promoterless pGL3-Basic vector (Promega). Our synthetic promoters ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were then incubated in species specific secondary HRP-conjugated antibody (anti-rabbit, Promega, W4011 and anti-mouse, Promega, W4021) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were then incubated in species specific secondary HRP-conjugated antibody (anti-rabbit, Promega, W4011 and anti-mouse, Promega, W4021) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the cDNA fragments corresponding to the coding region or 3′-UTR of mouse Inka2 cDNA were subcloned into a pGEM-3Z plasmid vector (Promega) for in vitro transcription ...
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bioRxiv - Cell Biology 2024Quote: ... the Deaf1 promoter region was first PCR-amplified from genomic DNAs of C2C12 mouse myoblasts and cloned into the pGL4.20 vector (Promega, E675A). ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed in 1×PBST and then incubated with the secondary antibody (anti-rabbit or anti-mouse IgG conjugated with horse radish peroxidase at 1:10 000, Promega). The membranes were then revealed using an ECL kit (GE Healthcare ...
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bioRxiv - Genomics 2023Quote: ... Primary antibodies were revealed using species-appropriate HRP-conjugated secondary antibodies (anti-rabbit or anti-mouse IgG (1:5000, Promega), or anti-guinea pig IgG (1:5000 ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from NMRI mouse cortical neuron cultures at DIV1 and DIV20 using the Reliaprep RNA Miniprep system (Promega) followed by deoxyribonuclease I (NEB ...
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bioRxiv - Genomics 2023Quote: Promoters were amplified from mouse genomic DNA using primers listed in table S1 and cloned into pGL3-Basic (E1751, Promega) using SacI and BglII.
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bioRxiv - Bioengineering 2024Quote: ... and incubated with the following primary antibodies diluted in TBS-T overnight at 4°C: mouse anti-HaloTag (1:5,000; Promega, G9211), mouse anti-Flag M2 (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... using CalPhos™ Mammalian Transfection Kit (Takara Bio or Promega) on DIV7 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the PCR products were cloned using the pGEMT kit (Promega): 3 μl PCR products were ligated to 50 ng pGEMT vector in the presence of 3 units T4 ligase overnight at 18°C (final volume 10 μL) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Locusta cDNA was synthesized using the cDNA Synthesis Kit (Promega). 2.5 ng of cDNA was used in each qPCR reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... An ImProm-II™ Reverse Transcriptase System kit (Promega, USA) was used to synthesize the first strand cDNA using 1.5 μg of the DNA-free RNA sample as a template and each reaction was carried out according to the supplier’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... cDNA was prepared using Promega kit (REF 017319, Promega, USA). Primers were designed according to the sequence information from the NCBI database using Primer3 Input (version V ...
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bioRxiv - Genomics 2020Quote: ... Assays were performed using the dualluciferase reporter assay kit (Promega) and a Veritas Microplate Luminometer (Turner Biosystems) ...
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bioRxiv - Immunology 2021Quote: The T Cell Activation Bioassay kit was purchased from Promega. The Jurkat-NFAT cells were thawed and cultured in RPMI (supplemented with 10% FBS and 5% pen/strep ...
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bioRxiv - Immunology 2021Quote: ... cDNA was then synthesized using AMV reverse transcriptase kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... coli S30 Extract System for Linear Templates Kit (Promega #L1030) as previously described (C ...
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bioRxiv - Cell Biology 2019Quote: ... purified chromatin was recovered using a PCR purification kit (Promega). Sequencing libraries were generated using standard methods and samples were sequenced on a MiniSeq instrument (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: A commercial kit (HDAC-Glo™ 2 Assays, Promega, G9590) was used to measure HDAC2 activity ...
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bioRxiv - Zoology 2019Quote: ... purified using Wizard SV Gel and PCR Cleanup kit (Promega), and sequenced directly (Sequetech ...
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bioRxiv - Microbiology 2019Quote: ... The Wizard genome DNA purification kit (Promega Corp., Madison, WI) was used for isolation of bacterial genomic DNA (gDNA) ...
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bioRxiv - Immunology 2019Quote: ... cDNA was generating using the GoScript Reverse Transcriptase kit (Promega). Quantitative-PCR was set up using the SensiFAST SYBR Hi-ROX kit (Bioline ...
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bioRxiv - Evolutionary Biology 2020Quote: ... using the Dual-Glo Luciferase Assay System Kit (Promega, US) as substrate ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was then synthesized using AMV reverse transcriptase kit (Promega) with Oligo-dT primer ...
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bioRxiv - Bioengineering 2021Quote: ... and collagen content via a QuantiFlour dsDNA assay kit (Promega), a modified 1,9-dimethylmethylene blue (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... the transfection was conducted using FuGENE6 kit (Cat#2619, Promega) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2020Quote: ... Luciferase assays were performed using Dual Luciferase Assay Kit (Promega) as described (Malecki et al. ...