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Citations for IBA Life Sciences :
1 - 50 of 112 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences), and SARS-CoV-2 spike-StreptTactin APC (StrepTactin APC from IBA Lifesciences).
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences), SARS-CoV-2 ΔRBM RBD-APC (streptavidin-APC from BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences), SARS-CoV-2 ΔRBM RBD-PE/Cy5.5 (streptavidin-PE/Cy5.5 from BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... and SARS-CoV-2 spike-StreptTactin APC (StrepTactin APC from IBA Lifesciences).
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bioRxiv - Molecular Biology 2022Quote: ... SARS-COV-2 NTD proteins were purified with Sepharose Strep-Tactin beads (IBA Life Sciences) as previously described [75].
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bioRxiv - Microbiology 2021Quote: ... Samples stained with trimeric SARS-CoV-2 RBD were incubated with primary antibody strepmabHRP (2-1509-001, IBA lifesciences) with specificity towards the TwinStrep-tag at the C-terminus of the trimeric protein for 120 hours in PBSGT with 0.1% saponin ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV S2P was further purified using a 2ml Strep-Tactin sepharose column (IBA Lifesciences Cat# 2-1201-002) and Strep-Tactin Purification Buffer Set (IBA Lifesciences Cat # 2-1002-001 ...
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bioRxiv - Molecular Biology 2021Quote: ... 2xStrep tagged SARS-CoV-2 ORFs were immunoprecipitated using 30 μl of Megastrep type 3 XT beads (IBA LifeSciences, Germany), and flag-tagged SARS-CoV-2 ORFs were immunoprecipitated using Anti-DYKDDDDK Magnetic Agarose (Pierce ...
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bioRxiv - Neuroscience 2021Quote: ... Twin-Strep-tag® (IBA Lifesciences; 2-1517-001; 1:1000); and Streptavidin 660 Conjugate (ThermoFisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... or anti-Strep-tag II antibody (IBA Lifesciences # 2-1507-001) and anti-mouse IgG1 antibody Alexa Fluor 647 (Thermo Fisher Scientific # A-21240) ...
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bioRxiv - Immunology 2023Quote: ... or anti-Strep-tag II antibody (IBA Lifesciences #2-1507-001) and anti-mouse IgG1 antibody Alexa Fluor 647 (Thermo Fisher Scientific #A-21240) ...
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bioRxiv - Biochemistry 2023Quote: 1 mL BioLock biotin blocking solution (cat# 2-0205-050, IBA Lifesciences) was added per 1.5 L of conditioned medium and incubated for 2 h gently stirring at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... StrepTactin XT beads (2-5010-025) and biotin (0-1016-002) were purchased from IBA Lifesciences (Germany). Dihydroartemisinin (DHA ...
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bioRxiv - Microbiology 2024Quote: ... 1mM EDTA and 2 mM biotin) and eluted with 3X volume of BXT buffer (IBA Life Sciences). Samples were run on 12% TGX gels (Biorad ...
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bioRxiv - Biochemistry 2023Quote: ... the SNAPf-EGFP-6His tag was replaced with a strep-II tag (IBA Lifesciences GmbH) using Q5 mutagenesis (New England Biolabs Inc. ...
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bioRxiv - Biochemistry 2023Quote: ... a biotin-blocking solution (IBA lifesciences) was added and incubated with the medium for 15 min at 4 °C under gentle stirring ...
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bioRxiv - Biochemistry 2022Quote: ... The spike ectodomains were purified using StrepTactin resin (IBA LifeSciences) and size exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2024Quote: ... The spike ectodomains were purified using StrepTactin resin (IBA LifeSciences) and size exclusion chromatography (SEC ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were used: StrepMAB-Immo (anti-Twin-Strep-tag®; IBA Lifesciences; 2-1517-001; 1:4000), FUS (1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... and spike was purified using Strep-Tactin XT beads (IBA Lifesciences), followed by size exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2024Quote: ... a Twin-Strep-tag (IBA Lifesciences, Göttingen) and ARID49-152 ...
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bioRxiv - Biochemistry 2021Quote: ... The spike ectodomain was purified using Strep-Tactin Superflow resin (IBA Lifesciences) followed by size exclusion chromatography using Superose 6 Increase column (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: Cells were analysed for receptor surface expression by flow cytometry using anti-Strep-tag II antibody Oyster 645 (IBA Lifesciences # 2-1555-050), or anti-Strep-tag II antibody (IBA Lifesciences # 2-1507-001 ...
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bioRxiv - Microbiology 2022Quote: ... incubated with anti-StrepMAB-Classic-HRP antibody that recognizes the strep tag at the C-terminus of the SFV Env ectodomain (7.5 μg/ml, IBA Lifesciences #2-1509-001) for 1 hour ...
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bioRxiv - Immunology 2023Quote: Cells were analysed for receptor surface expression by flow cytometry using anti-Strep-tag II antibody Oyster 645 (IBA Lifesciences #2-1555-050), or anti-Strep-tag II antibody (IBA Lifesciences #2-1507-001 ...
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bioRxiv - Immunology 2021Quote: ... or strep-tagged spike immobilized on Strep-Tactin XT magnetic beads (IBA Lifesciences). To select for non-RBD binders ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mL of BioLock biotin blocking solution (IBA Lifesciences) were added to 1.5 L conditioned medium ...
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bioRxiv - Cell Biology 2023Quote: ... pulldown buffer supplemented with 50mM biotin (IBA Lifesciences GmbH) formulated in elution buffer (1M Tris-Cl pH 8 ...
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bioRxiv - Immunology 2021Quote: ... The spike trimer was purified from filtered supernatant on Streptactin XT resin (IBA Lifesciences) or Ni-NTA resin and purified by size exclusion chromatography on a Superdex 200 ...
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bioRxiv - Immunology 2021Quote: ... The spike trimer was purified from filtered supernatant on Streptactin XT resin (IBA Lifesciences) or Ni-NTA resin and purified by size-exclusion chromatography on a Superdex 200 (Cytiva) ...
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bioRxiv - Immunology 2020Quote: ... The spike ectodomain was purified from filtered supernatant on Streptactin XT resin (IBA Lifesciences), followed by size-exclusion chromatography on a Superdex 200 in 5 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... Histones H2A.S2 (bearing a C-terminal StrepII-tag, IBA Lifesciences) and H2B were expressed individually ...
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bioRxiv - Cell Biology 2022Quote: ... StrepMAB-Classic DY-549 (IBA LifeSciences, FC 1:500), Strep-Tactin-DY649 (IBA LifeSciences ...
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bioRxiv - Molecular Biology 2022Quote: ... using α-strep-tag mouse antibodies 1:3000 (IBA Life Sciences). Subsequently ...
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bioRxiv - Immunology 2022Quote: ... pH 8.0 and supplemented with a biotin blocking solution (IBA Lifesciences). RBDs were purified by affinity chromatography on a Strep-Trap HP 5 ml column followed by elution with 50 mM biotin and buffer exchange into PBS ...
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bioRxiv - Bioengineering 2021Quote: ... Strep-tag and Strep-Tactin XT were purchased from IBA Lifesciences (Gottingen, Germany). Detergent compatible (DC ...
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bioRxiv - Biophysics 2022Quote: ... Mouse monoclonal antibody against the Strep tag (StrepMAB-Classic) was from IBA Lifesciences (Catalog # ...
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bioRxiv - Immunology 2020Quote: ... The (Twin)-Strep-tag /Strep-Tactin affinity purification system (IBA LifeSciences, Göttingen, Germany) was used to produce twin-strep-tagged fusion chemokines which were detected with an HRP-conjugated anti-Streptag mAb (IBA LifeSciences) ...
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bioRxiv - Microbiology 2024Quote: ... Spike proteins in culture medium were purified with gravity flow columns using Strep-Tactin XT (IBA Life Sciences), concentrated to 0.5 mg/ml with ultrafiltration tubes (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... Bound complex was eluted using Buffer BXT containing 50 mM biotin (IBA Lifesciences). Protein samples were diluted into SDS-PAGE lysis buffer and incubated at 99 °C for 10 min for separation by SDS-PAGE and Coomassie staining ...
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bioRxiv - Biochemistry 2019Quote: Fluorescently tagged Arc and StrepII tagged Arc were coexpressed in HEK293 cells and purified using Strep-Tactin Sepharose (IBA Lifesciences). Formation of complexes between the indicated Arc constructs was analyzed by immunoblotting following SDS electrophoresis ...
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bioRxiv - Immunology 2022Quote: ... Both S trimer and S2 trimer were expressed in the FreeStyle 293F cells and purified by Strep-Tactin Sepharose (IBA Lifesciences) followed by gel filtration chromatography (GE Healthcare) ...
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bioRxiv - Cell Biology 2024Quote: ... The Twin-Strep-tag® capture kit was from Iba Lifesciences (IBA GmbH, Germany). Peptides were purchased from Peptide 2.0 Inc (Chantilly ...
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bioRxiv - Biochemistry 2019Quote: ... 1 mM EDTA) and the kinase was eluted in 1x Biotin elution buffer BXT (IBA lifesciences). Plk1 was isolated from bacteria ...
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bioRxiv - Cell Biology 2022Quote: ... The Twin-Strep tag and Strep-Tactin XT protein fluorescently conjugated with DY-649 were purchased from IBA Lifesciences.
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bioRxiv - Biophysics 2022Quote: ... The proteins were purified via a C-terminal STREP II tag using Strep-tactin resin (IBA LifeSciences, Göttingen, DE). The solubilizing detergents were replaced by 0.06% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... the ZnF was expressed as a fusion construct with an N-terminal Twin-Strep-tag® (IBA Lifesciences, Göttingen) and purified as the other constructs.
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bioRxiv - Biochemistry 2021Quote: ... Protein detection on the blots was performed with an antibody against the Strep-tag II (StrepMAB-Classic, IBA Lifesciences, Germany).
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bioRxiv - Evolutionary Biology 2023Quote: ... Membranes were rinsed once with PBS-T (PBS with 0.01% Tween-20) and incubated with primary Strep-tag II antibody (Strep-MAB-Classic, IBA Lifesciences, Cat# 2-1507-001 ...
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bioRxiv - Biochemistry 2020Quote: ... LMNG and proteins were eluted in 5 CV of TBS supplemented with 0.01% (w/v) LMNG and 50 mM D-biotin (IBA Lifesciences). Eluates were concentrated using a 100-kDa molecular weight cutoff (MWCO ...