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Citations for IBA Life Sciences :
1 - 50 of 58 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Tumor cells were either kept untreated or were incubated with CMV pp65 antigen peptide (IBA Lifesciences) for 1 h at 37°C and washed once with medium ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike protein was expressed as described for RBD and NTD antigens in Expi293 cells and purified from mammalian cell culture supernatant using Strep-Tactin immobilized affinity resin (IBA Lifesciences). The bacterial expression construct for full-length SARS-CoV-2 nucleocapsid was a gift from Nicolas Fawzi (Addgene plasmid # 157867 ...
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bioRxiv - Neuroscience 2021Quote: ... Cell lysate was immunoprecipitated with Magstrep Type3 beads (IBA Lifesciences; 2-4090-002) overnight with end/end rocking at 4°C following the protocol provided by the manufacturer ...
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bioRxiv - Biochemistry 2021Quote: ... The cell lysate passed over a Strep-Tactin column (IBA Lifesciences, Goettingen, Germany) pre- equilibrated with a binding buffer (10 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Cancer Biology 2023Quote: ... Clarified cell lysates were incubated with prewashed Strep-Tactin beads (IBA Life Sciences) and allowed to bind for 2 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... the cell lysate was loaded onto preequilibrated Strep-Tactin XT 4Flow resin (IBA Lifesciences GmbH, Germany) and washed with wash buffer (500 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... Cell debris were pelleted and clear lysate was loaded on Strep-Tactin® resin (IBA Lifesciences) pre-equilibrated with Strep Buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... TWIN-Strep tagged proteins were then purified by incubating whole cell lysates from the transiently-transfected cell lines (0.5 ml) with 50 μl of MagStrep “type3” XT beads (IBA Life Sciences) for two-four hours at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: Strep tagged proteins were then purified by incubating whole cell lysates with MagStrep “type3” XT beads (IBA Life Sciences) for five-six hours at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates from cells transfected with Sb1 were directly loaded onto 100-300 µl of Streptactin superflow resin (IBA LifeSciences) to purify SBP-tagged sybodies ...
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bioRxiv - Molecular Biology 2020Quote: ... lysates were incubated with Strep-Tactin beads (IBA Lifesciences) at 4°C overnight ...
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bioRxiv - Cell Biology 2019Quote: ... clarified lysate was cycled over a Strep column (IBA Lifesciences) for 2 hr at 4 °C ...
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bioRxiv - Biophysics 2019Quote: ... The lysate was passaged on a Streptactin XT resin (IBA lifesciences) equilibrated with lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Lysate was loaded on Streptactin-XT resin (IBA Lifesciences, Göttingen, Germany) (bead volume ...
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bioRxiv - Cell Biology 2023Quote: Lysate was loaded on Streptactin-XT resin (IBA Lifesciences, Göttingen, Germany) (bead volume ...
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bioRxiv - Biochemistry 2024Quote: ... Lysate was loaded on Streptactin-XT resin (IBA Lifesciences, Göttingen, Germany) (bead volume ...
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bioRxiv - Systems Biology 2023Quote: ... the lysate was incubated with MagStrep ‘type3’ beads (30 μl; IBA Lifesciences) for 2 h at 4C ...
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bioRxiv - Biophysics 2021Quote: ... The TS-SUMO-SaCas9-sgRNA or TS-SUMO-SaCas9 cell lysate was applied to a 5 mL Strep-Tactin Superflow 50% suspension (IBA Life Sciences, 2-1206-025) gravity column equilibrated in Lysis Buffer containing 200 mM NaCl ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs from HLA-A*0201 Donors were either stimulated with 1 µg/mL CMV pp65 antigen peptide NLVPMVATV (HLA-A*0201) (IBA Lifesciences) for 1 h or not ...
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bioRxiv - Biochemistry 2020Quote: ... and lysates were incubated with 100 μl Streptactin-sepharose (IBA Lifesciences, Göttingen, Germany) for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Spin-cleared lysate was loaded into a Strep-Tactin Sepharose resin (IBA Lifesciences) and washed with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... the cleared lysate was incubated with 1.5 mL StrepTactin Sepharose beads (IBA Lifesciences) for 1 hour at 4°C on a rotator ...
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bioRxiv - Microbiology 2024Quote: ... Cleared lysates were then incubated with 1.5 mL of StrepTactin Sepharose beads (IBA Lifesciences) for 1 hour at 4°C on a rotator ...
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bioRxiv - Biochemistry 2023Quote: Lysate was loaded on 5 ml of Streptactin-XT resin (IBA Lifesciences, Göttingen, Germany) and passed through the resin by gravity flow ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was mixed with 2 mL Strep-Tactin XT resin (IBA Lifesciences, Göttingen, Germany). The resin was washed extensively with wash buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2019Quote: ... The lysate was mixed with 2 ml Strep-Tactin XT resin (IBA Lifesciences, Göttingen, Germany). The resin was washed with wash buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates cleared by centrifugation were incubated with Strep-Tactin® Sepharose resin (IBA Lifesciences). Purified proteins were then eluted by desthiobiotin using Buffer E (IBA Lifesciences ...
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bioRxiv - Cancer Biology 2023Quote: ... twin-strep tagged MKM containing lysates were incubated with Streptactin XT magnetic bead slurry (IBA Lifesciences) and purified according to manufacturer’s recommendation ...
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bioRxiv - Biophysics 2021Quote: ... Clarified lysate was placed on a Strep-Tactin Superflow 50% suspension (IBA Life Sciences, 2-1206-025) gravity column equilibrated in AcrIIA11 Lysis Buffer ...
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bioRxiv - Biochemistry 2020Quote: ... LMNG-solubilized lysates were added to 5 μg of TBS-prewashed MagStrep XT magnetic beads (IBA Lifesciences) for 1 h with mild shaking ...
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bioRxiv - Microbiology 2022Quote: ... and then 1mg of lysate was incubated with prewashed MagStrep “type 3” XT magnetic beads (IBA LifeSciences) overnight in IP buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2024Quote: ... then the soluble lysate was incubated with 1mL equilibrated Strep-Tactin® Sepharose® resin (IBA Lifesciences) rotating for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Viral proteins were affinity purified from 4mg lysates by tumbling with 40µl Strep-Tactin Sepharose (IBA Life Sciences) for 90min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Lysate was cleared by centrifugation and the supernatant was incubated with a Strep-Tactin Sepharose resin (IBA Lifesciences). Resin was thoroughly washed with lysis buffer and 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and SBP-tagged proteins were affinity purified using 5 ml of Streptactin superflow resin (IBA LifeSciences). The resin was washed with 40 column volumes (CV ...
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bioRxiv - Biochemistry 2023Quote: ... and the clarified lysate was then loaded onto a gravity flow column packed with Strep-tactin XT agarose beads (Iba Lifesciences). After binding ...
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bioRxiv - Biophysics 2021Quote: ... Cellular debris was pelleted by ultracentrifugation before placing lysate over a Strep-Tactin Superflow 50% suspension (IBA Life Sciences, 2-1206-025) gravity column equilibrated in Lysis Buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Insoluble materials were discarded and clear lysates were added to Strep-Tactin sepharose (400 μL 50% slurry; IBA Lifesciences #2-1201-010) loaded columns (Bio-Rad Laboratories #732-6008 ...
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bioRxiv - Microbiology 2024Quote: ... 0.5-1 mg of lysate was incubated overnight with 30 ul of Strep-Tactin Sepharose resin (IBA Life Science, 2-1201-002), rotating at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... The cleared lysate was loaded onto a gravity-flow column with Strep-Tactin® Superflow® high capacity resin (IBA Lifesciences GmbH) pre-equilibrated with Buffer W ...
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bioRxiv - Cell Biology 2024Quote: ... TwinStrep-tagged: The lysate was passed through chromatography columns containing 1.5 ml bed volume of Strep-Tactin® Sepharose® resin (IBA Lifesciences, Germany). Resin was washed with ∼20 resin volumes of 100 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... MEXi 293E cells (IBA Lifesciences) were cultured in MEXi culture medium (IBA Lifesciences ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli cells (5-1600-020, IBA Life Sciences, Göttingen). Transformants were screened by colony PCR and verified by sequencing (StabVida ...
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bioRxiv - Developmental Biology 2022Quote: ... the cell culture supernatants were applied to a Strep-tactin column (IBA Lifesciences GmbH, Göttingen, Germany) and eluted with 2.5 mM desthiobiotin ...
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bioRxiv - Cell Biology 2022Quote: Transfection of 3T3 cells was carried out using the MatraTM magnetic bead transfection system (IBA Lifesciences) to warrant nuclear membrane integrity for subsequent FRAP experiments ...
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bioRxiv - Immunology 2021Quote: ... before staining cells with DY-549-labeled strep-tactin (1:100 dilution, IBA lifesciences, Cat #2-1565-050) or Allphycocyanin-labeled streptavidin (1:200 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... After equilibration of the Strep-tactin XT Superflow resin (IBA lifesciences, 10 mL per liter of cell supernatant) in 0.1 M Tris-HCl pH 8 ...
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bioRxiv - Immunology 2024Quote: ... B cells were stained with rGP38 (IbAr10200) that had been tetramerized at 25 nM using Streptactin-PE (IBA Lifesciences) and Streptactin-APC (IBA Lifesciences) ...
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bioRxiv - Biochemistry 2019Quote: Fluorescently tagged Arc and StrepII tagged Arc were coexpressed in HEK293 cells and purified using Strep-Tactin Sepharose (IBA Lifesciences). Formation of complexes between the indicated Arc constructs was analyzed by immunoblotting following SDS electrophoresis ...
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bioRxiv - Immunology 2021Quote: Transfected cells were sorted for HDR+ by staining for Strep-Tactin-APC 1:100 (IBA lifesciences, Cat: 6-5010-001) and anti-hIgG AF488 1:100 (Jackson ImmunoResearch ...