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Citations for IBA Life Sciences :
1 - 50 of 107 citations for Angiotensin II receptor AT 2R Rabbit Polyclonal affinity purified since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and purified by StrepTactin affinity chromatography (IBA Lifesciences). To generate baculoviruses ...
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bioRxiv - Biophysics 2023Quote: ... the protein was purified using Streptactin affinity resin (IBA Life Sciences). Resin bound protein was washed as before and eluted using wash buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... The AQP7 protein was then purified by affinity chromatography using Strep-Tactin Superflow (IBA Lifesciences). The AQP7 was further purified with gel-filtration using superdex 200 10/300 Gel column (GE lifescience ...
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bioRxiv - Microbiology 2024Quote: ... ReoM-Strep was purified using affinity chromatography and Strep-Tactin® Sepharose (IBA Lifesciences, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Strep-tagged proteins were purified using affinity chromatography and Strep-Tactin® Sepharose (IBA Lifesciences, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The strep tagged protein was purified using affinity chromatography and Strep-Tactin Sepharose (IBA Lifesciences, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Viral proteins were affinity purified from 4mg lysates by tumbling with 40µl Strep-Tactin Sepharose (IBA Life Sciences) for 90min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were further purified by loading His60 eluate onto Strep-TactinXT affinity chromatography gravity columns (IBA Lifesciences, Germany). Final pure protein was eluted with Buffer BXT +10% glycerol (IBA Lifesciences ...
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bioRxiv - Immunology 2019Quote: Cells were analysed for receptor surface expression by flow cytometry using anti-Strep-tag II antibody Oyster 645 (IBA Lifesciences # 2-1555-050), or anti-Strep-tag II antibody (IBA Lifesciences # 2-1507-001 ...
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bioRxiv - Immunology 2023Quote: Cells were analysed for receptor surface expression by flow cytometry using anti-Strep-tag II antibody Oyster 645 (IBA Lifesciences #2-1555-050), or anti-Strep-tag II antibody (IBA Lifesciences #2-1507-001 ...
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bioRxiv - Biophysics 2023Quote: ... the tagged d1_d2 was purified from the supernatant by affinity chromatography using Strep-Tactin resin (IBA Lifesciences, Göttingen, Germany).
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bioRxiv - Biochemistry 2022Quote: ... the Ldh-EtfAB complex was purified by affinity chromatography on Strep-Tactin high-capacity material (IBA Lifesciences GmbH, Göttingen, Germany) using the elution buffer E (buffer W+ 5 mM desthiobiotin) ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike protein was expressed as described for RBD and NTD antigens in Expi293 cells and purified from mammalian cell culture supernatant using Strep-Tactin immobilized affinity resin (IBA Lifesciences). The bacterial expression construct for full-length SARS-CoV-2 nucleocapsid was a gift from Nicolas Fawzi (Addgene plasmid # 157867 ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were purified from each collected supernatant by affinity chromatography using StrepTactin XT 4Flow® 5 mL cartridges (Iba Lifesciences). Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck) ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and SBP-tagged proteins were affinity purified using 5 ml of Streptactin superflow resin (IBA LifeSciences). The resin was washed with 40 column volumes (CV ...
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bioRxiv - Microbiology 2023Quote: ... supernatant from cells incubated with 4 µM CdCl2 for 5 days was collected and soluble protein was purified by affinity chromatography using a Strep-Tactin XT 4Flow column (IBA Lifesciences) followed by size exclusion chromatography using a HiLoad 26/600 superdex 200 pg column (Cytiva ...
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bioRxiv - Biochemistry 2019Quote: The BBS2-7-9 complex was purified at 4°C by affinity purification using 4 mL of Strep-Tactin resin (IBA Life Sciences) packed in a 2 cm diameter column and equilibrated with equilibration buffer (20 mM HEPES pH 7.5 and 20 mM NaCl) ...
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bioRxiv - Biochemistry 2021Quote: ... with half immunoprecipitated for FLAG-ATF6 with 15 μL G1 anti-DYKDDDK resin and the other half affinity purified for nsp3.1-ST using 15 μL Strep-Tactin XT Superflow High Capacity resin (IBA Life Sciences, 2-4030-002) and rocked at 4°C overnight ...
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bioRxiv - Biophysics 2021Quote: ... followed by Streptactin affinity chromatography (IBA Lifesciences, Germany). Purified CKAP2 was eluted with BRB80 (80 mM PIPES-KOH pH 6.85 ...
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bioRxiv - Bioengineering 2022Quote: ... Strep-Tactin XT affinity beads (IBA Life Sciences) were spun down and washed twice with PBS at 300 rpm for 60 s ...
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bioRxiv - Biophysics 2022Quote: ... The proteins were purified via a C-terminal STREP II tag using Strep-tactin resin (IBA LifeSciences, Göttingen, DE). The solubilizing detergents were replaced by 0.06% (w/v ...
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bioRxiv - Biophysics 2019Quote: ... APC/C was expressed with a Twin-Strep(II)-tag on the C-terminus of APC4 and purified using Strep-Tactin Sepharose (IBA Life Sciences) affinity chromatography followed by ion exchange chromatography and SEC ...
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bioRxiv - Cell Biology 2023Quote: Affinity purification was performed with MagStrep type3 XT beads (IBA Lifesciences) as recommended by the manufacturer ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on Streptactin Superflow affinity resin (IBA Lifesciences) and incubated for 2 h under gentle agitation ...
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bioRxiv - Biochemistry 2024Quote: ... Affinity chromatography was performed with Strep-TactinTMXT 4FlowTM resin (IBA Lifesciences), and the protein eluted with 100 mM Tris/HCl buffer containing 150 mM NaCl and 50 mM biotin ...
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bioRxiv - Molecular Biology 2021Quote: ... and applied to Strep-Tactin affinity chromatography column (IBA Lifesciences, #2-1201-025) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... The (Twin)-Strep-tag /Strep-Tactin affinity purification system (IBA LifeSciences, Göttingen, Germany) was used to produce twin-strep-tagged fusion chemokines which were detected with an HRP-conjugated anti-Streptag mAb (IBA LifeSciences) ...
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bioRxiv - Biophysics 2023Quote: ... The solubilized supernatant was passed through Strep-Tactin affinity resin (IBA Life Sciences) column at a flow rate of ∼0.3-0.5 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity chromatography was performed with Strep-Tactin Sepharose slurry (1 mL/L) (IBA Lifesciences), using 1xTris buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Systems Biology 2021Quote: ... and Streptactin II beads (IBA Lifesciences) were used ...
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bioRxiv - Molecular Biology 2021Quote: ... and applied to Strep-Tactin affinity chromatography according to the manufacturer’s protocol (IBA Life Sciences). The protein was eluted with buffer D containing 5 mM d-Desthiobiotin ...
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bioRxiv - Biophysics 2023Quote: ... starting with affinity chromatography using either a Strep-Tactin column (Strep-Tactin Superflow, IBA Lifesciences) or an Ni-IMAC (Cytiva/ Ni-NTA Agarose ...
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bioRxiv - Immunology 2023Quote: ... Stepwise purification included affinity chromatography using StrepTrap HP (Cytiva) ran in 1X Buffer W (IBA Lifesciences) and eluting in 1X Buffer E (IBA Lifesciences) ...
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bioRxiv - Molecular Biology 2020Quote: ... High purity monomeric sE2 was generated by sequential affinity purification using StrepTactin-XT columns (IBA Lifesciences, Göttingen, Germany) and size-exclusion chromatography using the HiPrep™ 16/60 Sephacryl® S-200 HR gel filtration column (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... purified using StrepTactin resin (IBA LifeSciences) and size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was subjected to affinity chromatography using 1 mL buffer equilibrated Strep-Tactin XT 4Flow resin (IBA Lifesciences-GmbH) and flow through was collected ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... loaded onto a streptactin II column (IBA Life Sciences, Germany), washed with 10 column volumes to remove unbound protein ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: nProtein A Sepharose 4 Fast Flow resin (Cytiva) was used for affinity purification of adalimumab whilst Strep-Tactin XT 4Flow resin (IBA Lifesciences) was used for Strep-II tagged-tagged proteins ...
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bioRxiv - Cancer Biology 2022Quote: We transfected pStrep-ANKLE1 plasmid into HEK293T cells for 24 hours and performed affinity purification MagStrep3XTBeads and BXT elution buffer (IBA Lifesciences) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Affinity purification of Strep3-tagged proteins was performed using a Strep-Tactin® Superflow® high capacity FPLC column (IBA Lifesciences).
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bioRxiv - Immunology 2022Quote: ... and purified by Strep-Tactin Sepharose (IBA Lifesciences) followed by gel filtration chromatography (GE Healthcare) ...
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bioRxiv - Immunology 2019Quote: ... or anti-Strep-tag II antibody (IBA Lifesciences # 2-1507-001) and anti-mouse IgG1 antibody Alexa Fluor 647 (Thermo Fisher Scientific # A-21240) ...
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bioRxiv - Immunology 2023Quote: ... or anti-Strep-tag II antibody (IBA Lifesciences #2-1507-001) and anti-mouse IgG1 antibody Alexa Fluor 647 (Thermo Fisher Scientific #A-21240) ...
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bioRxiv - Immunology 2023Quote: ... were purified over Strep-Tactin Sepharose resin (IBA Lifesciences) in a gravity column ...
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bioRxiv - Molecular Biology 2024Quote: ... then purified by Strep-Tactin Sepharose resin (IBA Lifesciences), washed with 5 column volumes of 1X PBS ...
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bioRxiv - Biochemistry 2020Quote: ... Purification of Nth1-Strep was performed using Strep-Tactin II Superflow (IBA lifesciences). Clb2-Cdk1 was purified from yeast cells overexpressing Clb2-TAP as described previously (52,53).
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bioRxiv - Biochemistry 2022Quote: ... Proteins were purified using Strep-Tactin Superflow resin (IBA Lifesciences) employing four washing steps ...
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bioRxiv - Biochemistry 2022Quote: ... The spike ectodomains were purified using StrepTactin resin (IBA LifeSciences) and size exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2024Quote: ... The spike ectodomains were purified using StrepTactin resin (IBA LifeSciences) and size exclusion chromatography (SEC ...
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bioRxiv - Immunology 2023Quote: ... Fabs were purified using StrepTactin 4Flow resin (IBA Life Sciences) or Ni-NTA resin (Thermo Scientific) ...