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Citations for iNtRON Biotechnology :
1 - 34 of 34 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and reverse-transcribed using RT-Premix (Intron Biotechnology). PCR was performed with the following primers (the respective forward and reverse pairs are indicated) ...
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bioRxiv - Cell Biology 2022Quote: ... PCR product was obtained with Maxime PCR Premix (i-pfu) (Intron Biotechnologies). pLPCX vector was digested and then dephosphorylated before ligation using the Rapid DNA Dephos & Ligation Kit (Roche) ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA (1 µg) was used for cDNA synthesis with RT PreMix (Intron Biotechnology, Seoul, Korea) containing oligo dT primer according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized (from 5μg RNA) with a Maxime RT PreMix kit (iNtRON Biotechnology, South Korea) and real-time PCR was performed (from 1 μg of synthesized cDNA ...
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bioRxiv - Immunology 2023Quote: ... Extracted RNA was used for synthesizing complementary DNA (cDNA) using RT-premix (Intron Biotechnology, Seoul, Korea) containing an oligo-dT primer ...
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bioRxiv - Microbiology 2019Quote: ... (40) PCR amplification was carried out with Maxime PCR PreMix Kit (i-Taq) (iNtRON BIOTECHNOLOGY, Seongnam, Korea) and a PCR thermocycler (SensoQuest ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were verified as mycoplasma-free by PCR (e-Myco plus Mycoplasma PCR Detection Kit, iNtRON Biotechnology). Cells were seeded to achieve 80% confluence on the day they were infected with virus stocks listed in (23) ...
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bioRxiv - Immunology 2023Quote: ... The extracted RNA was used to synthesize complementary DNA (cDNA) using RT-premix (Intron Biotechnology, Seoul, Korea) containing an oligo-dT primer ...
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bioRxiv - Immunology 2019Quote: ... 1 μL (10 pmol) of each primer and 0.5 μL of Taq-polymerase 2,5 U (iNtRON Biotechnology, Inc). The program consisted of one cycle at 94°C 1 min ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNAs of PRRSV and CSFV were then prepared using Maxime™ RT PreMix Kit (iNtRON Biotechnology Inc., Korea). The genomic DNA of ASFV (1 pg) ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized from 1 μg of total RNA with a Maxime RT PreMix kit (iNtRON Biotechnology, Gyeonggi, Korea) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... The PCR products were visualized under UV illumination and excised from the gel and the PCR product was extracted using MEGAquick-spin PCR & Agarose Gel Extraction kit (iNtRON Biotechnology). PCR product was sequenced using forward and reverse primers (1st Base Co. ...
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bioRxiv - Molecular Biology 2023Quote: ... (e-Myco VALiD Mycoplasma PCR Detection Kit, iNtRON Biotechnology).
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bioRxiv - Molecular Biology 2023Quote: ... An e-Myco VALiD Mycoplasma PCR Detection Kit (iNtRON Biotechnology) was used to confirm that the culture was free of Mycoplasma.
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bioRxiv - Microbiology 2020Quote: ... The PCR was carried out using PreMix Mastermix kit (iNtRON Biotechnology) in 25 μL reaction mixture containing 13 μL distill water ...
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bioRxiv - Neuroscience 2019Quote: ... Contamination with mycoplasma was evaluated by PCR (eMyco kit, Intron Biotechnology). After 24h of transfection Doxycycline was added and experiments were performed.
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bioRxiv - Immunology 2019Quote: ... total RNAs were resuspended in nuclease-free water and cDNAs were synthesized from ~1 μg of RNAs using Maxime RT Premix (Intron Biotechnology, Seoul, Korea) according to the manufacturer’s instruction.
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were purified with MEGAquick-spinTM Total Fragment DNA Purification Kit (iNtRON Biotechnology) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were confirmed to be mycoplasma-negative (e-Myco Mycoplasma PCR Detection Kit, iNtRON Biotechnology). Passage was carried out using 0.25% trypsin-EDTA (GIBCO ...
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bioRxiv - Cell Biology 2023Quote: Cells were confirmed to be mycoplasma-negative (e-Myco plus Mycoplasma PCR Detection Kit, iNtRON Biotechnology).
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bioRxiv - Cell Biology 2020Quote: All cell lines were confirmed to be mycoplasma-negative (e-Myco Mycoplasma PCR Detection Kit, iNtRON Biotechnology). Passage was carried out using 0.25% trypsin-EDTA (GIBCO ...
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bioRxiv - Molecular Biology 2019Quote: ... Annealing temperature was adjusted using Maxime PCR PreMix Kit (i-Taq) 20 μl (INTRON Biotechnology, South Korea) on several runs of PCR ...
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bioRxiv - Physiology 2019Quote: ... The PCR amplification was performed with i-StarTaq DNA polymerase kit (iNtRON Biotechnology, Sungnam, Kyungki-Do, Korea) using 1 μl of a template (10 ng μl−1 ...
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bioRxiv - Molecular Biology 2023Quote: ... The Mycoplasma-free culture was confirmed by an e-Myco VALiD Mycoplasma PCR Detection Kit (iNtRON Biotechnology). A disposable cultivation chamber (DCC ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were then run on a 1% agarose gel containing RedSafe Nucleic Acid Staining Solution (Intron Biotechnology) and visualized with an EpiChem II Darkroom Transilluminator (UVP Laboratory).
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were detected by agarose gel electrophoresis using RedSafe™ Nucleic Acid Staining Solution (iNtRON Biotechnology, Inc.).
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bioRxiv - Molecular Biology 2021Quote: ... All cell lines were tested for mycoplasma contamination using e-MycoTM mycoplasma detection PCR kit (iNtRON Biotechnology, Inc., cat# 25235).
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bioRxiv - Molecular Biology 2023Quote: ... The cell culture was routinely tested for Mycoplasma contamination with the e-Myco VALiD Mycoplasma PCR Detection Kit (iNtRON Biotechnology) and confirmed to be negative.
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bioRxiv - Bioengineering 2021Quote: ... and Mycoplasma testing within 6 months of use with the e-Myco mycoplasma PCR detection kit (iNtRON Biotechnology Inc, Boca Raton, FL). Cell lines were maintained in culture at 37°C in 5% CO2.
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bioRxiv - Microbiology 2023Quote: ... and amplicons approximately 2–3 kb in size were excised from the gel and purified using the Quick-spin PCR Product Purification Kit (iNtRON Biotechnology, Korea). The amplicons were further cloned in pJET1.2 cloning plasmid (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Flies head were isolated as described in the qRT-PCR section and total protein was extracted using PRO-PREP kit (iNtRON Biotechnology, South Korea) according to the manufacturer protocol ...
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bioRxiv - Plant Biology 2022Quote: ... run on a Bio-Rad T100 Thermal Cycler (Hercules, CA, USA) with a PCR Master mix Solution i-MAX II (iNtRON Biotechnology, S Korea). PCR products were separated on a 1% agarose gel.
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bioRxiv - Cell Biology 2022Quote: ... All cultured cell lines were routinely tested for mycoplasma contamination every 3 months using a e-Myco VALID Mycoplasma PCR Detection Kit (iNtRON Biotechnology, Inc., cat# 25239).
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bioRxiv - Genomics 2023Quote: ... All cell lines were mycoplasma tested prior to freezing down stocks and prior to experiments using the e-Myco™ VALiD Mycoplasma PCR Detection Kit (iNtRON Biotechnology, Inc., CAT #25239).