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Citations for MP Biomedicals :
601 - 650 of 664 citations for Rat Activated Leukocyte Cell Adhesion Molecule CD166 ALCAM ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently washed with PBS and resuspended in serum- free RPMI with 3ug/mL Beta-2-microglobulin (MP Biomedicals) and the indicated concentration of peptide for 3 hours at room temperature followed by 3 hours at 37C in 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... MDCK cells expressing IAV-NS1 (MDCK-NS1 cells) were infected with IAVdelNS1 in EMEM containing 0.35% bovine serum albumin (BSA, MP Biomedicals), 4 mM L-glutamine ...
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bioRxiv - Biochemistry 2023Quote: ... Resuspended cells were placed in tubes containing 0.1-mm silica beads (Lysing matrix B tubes, catalog no. 6911-100; MP Biomedicals) and lysed in a bead beater (Bead Ruptor 24 Elite ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 500 µL of 0.5 mm diameter glass beads were added and cells were lysed by 8 runs of 40 sec of bead beating (6.5m/sec, FastPrep-24, MP Biomedicals) with cooling on ice between each round ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The cells were lysed mechanically by addition of 0.1 mm glass beads and using the Fastprep24 (MP Biomedicals, USA-CA) at a strength of 6.0 for 30 s for four cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... One mL of each TRIzol cell suspensions was transferred into 2 mL tubes with 0.5 mL zirconium beads (0.1 – 0.2 µm in diameter) and cells were disrupted by shaking employing a FastPrep-24 bead beating instrument (MP Biomedicals) 2 x 30 s at 6 m/s ...
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bioRxiv - Microbiology 2021Quote: ... Total genomic DNA was extracted using an MoBio PowerFecal® DNA Isolation Kit (Cat # 12830-50, MO BIO Laboratories, CA, USA) and FastPrep-24™ (MP Biomedicals, LLC, Irvine, CA, USA).
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bioRxiv - Cell Biology 2020Quote: ... a 50-μL aliquot of cells from sporulation culture was pelleted and resuspended in 1 mg/mL Zymolyase-20T (#320921, MP Biomedicals) dissolved in water and incubated at 30°C for 10 minutes.
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bioRxiv - Cell Biology 2020Quote: Yeast cells were grown either in rich medium (YPD) or in enriched complete synthetic medium (2x final concentration of CSM, MP Biomedicals) supplemented with 2% glucose or 2% galactose (wt/vol) ...
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bioRxiv - Developmental Biology 2021Quote: ... Membrane potentials (Vm) were monitored using the perforated whole-cell patch-clamp method with β-escin (MP Biomedicals, Santa Ana, CA) as the perforating agent (6 to 8 μM) ...
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bioRxiv - Cell Biology 2022Quote: ... The following day the media on the cells were replaced with media containing a combination of manganese chloride (MP Biomedicals, 155334) and Actinonin (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... A 0.5 mL aliquot of the cell suspension was transferred to a FastPrep Lysing Matrix B (MP Biomedicals, Santa Ana, CA) tube containing 0.5 mL of PBS (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: For protein extraction, cells were lysed (in 1 % sodium deoxycholate, 50 mM triethylammonium bicarbonate) using the FastPrep-96 Instrument (MP Biomedicals) for 1 min at 1600 rpm and the All FastDNA-96 (MP Biomedicals ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were transduced with lentiviral particles at MOI of 10 in a 150 μL of cell culture media supplemented with 10 μg/mL of protamine sulfate (#02194729, MP Biomedicals) and saved as ReN-CAG-BirA ...
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bioRxiv - Systems Biology 2020Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation using LSM Lymphocyte Separation Medium (MP Biomedicals, Santa Ana, CA). PBMCs were enriched for total monocytes using the Pan Monocyte Isolation Kit (Miltenyi Biotech ...
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bioRxiv - Microbiology 2022Quote: ... 600 μl of Trizol reagent was added and cells were then mechanically disrupted using a MP Biomedicals FastPrep Instrument and Lysing Matrix B tubes (MP Biomedicals). Black carbon particles were removed by centrifugation at 12 ...
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bioRxiv - Microbiology 2021Quote: ... pPol-I firefly Luc (NP gene; 100ng) and SV40 Renilla (50ng) reporter into HEK293T cells using polyethylenimine reagent (PEI, MP Biomedicals) at a ratio of 1:5 (DNA:PEI) ...
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bioRxiv - Microbiology 2021Quote: ... 1 x protease inhibitor (HALT cocktail, ThermoScientific) after the lysate was prepared following cell lysis via bead-beating (Fastprep Pro, MP Biomedicals). Immunoprecipitations were performed on 2-3 biological replicates.
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMC) were isolated from heparinized blood samples of human healthy donors using Ficoll-Paque density centrifugation (MP biomedicals), frozen and then stored in liquid nitrogen ...
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bioRxiv - Immunology 2020Quote: ... resuspended at 5×108 bacteria /mL and lysed using a FastPrep-24 Tissue and Cell Homogenizer in Lysing Matrix B tubes (MP Biomedicals) with a setting of 4.0 m/s for 20s ...
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bioRxiv - Immunology 2022Quote: ... Transduction was carried out two times for 24 hours and transduced cells were selected with appropriate antibiotics (5 μg/ml blasticidin, MP Biomedicals, Santa Ana ...
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bioRxiv - Molecular Biology 2022Quote: ... were added to the pellet and bead-beaten at 6m/s for 30 seconds using an MP Fast Prep-24 Tissue and Cell Homogenizer (MP Biomedicals).
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bioRxiv - Microbiology 2022Quote: ... Harvested cells suspended in 100 μl of lysis buffer (74) were mechanically disrupted in FastPrep Lysis Matrix A tubes (MP Biomedicals) at 30 Hz for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and lysis was carried out by 6 x 1min maximum speed cycles on a FastPrep-24™ cell homogeniser (MP Biomedicals), with the samples being returned to ice for 5min between each cycle ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were distributed to two screw-cap microtubes containing 300 µl glass beads and lysed in a FastPrep instrument (MP Biomedicals) at 3 x 45 s 6 m/s ...
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bioRxiv - Immunology 2024Quote: To further label viral RNA, cells were washed twice in Hybridization Wash Buffer (HWB) (2X SSC, 10% formamide (MP Biomedicals FORMD002), 0.2 mg/mL Bovine Seric Albumin (BSA ...
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bioRxiv - Microbiology 2022Quote: ... the larvae/embryos were mixed with 100 μL PBS in 1.5 mL tubes and homogenized with 3 cycles of 15 s at 6,500 rpm with rest intervals of 60 s by FastPrepTM 24-5G tissue-cell homogenizer (MP Biomedicals, USA). The homogenates were clarified by centrifugation at 9,000 × g for 5 min and the supernatants were used as the virus suspension for RT-qPCR analysis or further investigation (passaging of viruses by being injected to new zebrafish embryos ...
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bioRxiv - Cell Biology 2023Quote: ... 10 μM ZnSO4 and 2 mM PMSF and cells were lysed by glass bead lysis in the FastPrep-24 (MP Biomedicals). Pho8 activity was monitored by incubation of 200 μl lysate with 50 μl buffer 250 mM Tris–HCl ...
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bioRxiv - Microbiology 2023Quote: ... Cells were disrupted by bead beating for 40 seconds at 6.5m/s in a FastPrep-24 instrument (MP Biomedicals, United states). The supernatant was transferred to an Eppendorf tube and chloroform (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... propagation of the virus was carried out in Vero (African green monkey kidney epithelial – ATCC: CCL-81) cells cultured in MEM (MP Biomedicals), supplemented with 1 µg/mL TPCK-Trypsin (Trypsin-Worthington) ...
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bioRxiv - Cell Biology 2023Quote: ... 500 µl acid-washed glass beads were added and cells were mechanically lysed twice in a FastPrep device (6m/sec for 40 seconds; MP Biomedicals), with a 5-minute incubation on ice in between ...
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bioRxiv - Cell Biology 2023Quote: ... 300 µl acid-washed glass beads were added and cells were mechanically lysed twice with a FastPrep device (6m/sec for 40 seconds; MP Biomedicals) with a 5-minute incubation on ice in between ...
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bioRxiv - Cancer Biology 2023Quote: ... Stably transfected or lentivirally transduced cells were selected and maintained in the same medium supplemented with 0.5 to 1 µg/mL puromycin (MP Biomedicals 194539) or 4 µg/mL blasticidin (US Biological Life Sciences B2104-30) ...
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bioRxiv - Cell Biology 2023Quote: ... 200 µl of acid-washed beads were added and cells were mechanically lysed twice in a FastPrep device (6m/sec for 40 seconds; MP Biomedicals), with a 5-minute incubation on ice in between ...
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bioRxiv - Microbiology 2023Quote: ... Only the initial cell lysis step was carried out in a FastPrep-24 instrument in lysing matrix A tubes (both MP Biomedicals) under different settings for the supernatant and cellular fraction samples ...
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bioRxiv - Immunology 2023Quote: ... DMEM (for labeling HeLa cells) or RPMI minus leucine (RPMI without L-leucine, L-glutamine, and sodium pyruvate from MP Biomedicals, supplemented with Glutamax and 1mM sodium pyruvate ...
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bioRxiv - Cell Biology 2024Quote: ... Lysis was performed by six cycles of one minute at maximum speed on a FastPrep-24™ cell homogenizer (MP Biomedicals), with a 5 minutes cooling period on ice between each cycle ...
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bioRxiv - Cancer Biology 2022Quote: ... Viral infections were carried out over 72 hours and transduced cells were selected by their resistance to 2 μg/mL puromycin (MP Biomedicals 0219453910).
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bioRxiv - Neuroscience 2021Quote: ... 50,000/well freshly dispersed anterior pituitary cells were plated on poly-L-lysine-coated 8-well multitest slides (MP Biomedicals, Aurora, OH), bathed in Earle’s medium 199 supplemented with 2.2 g/L sodium bicarbonate ...
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bioRxiv - Cell Biology 2021Quote: ... for SA14-14-2 endogenous pools of cysteine and methionine were depleted by treating cells for 2 h with Cys− Met− MEM (MP Biomedicals #1641454) containing 7.5 μg/ml Actinomycin-D (AMD ...
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bioRxiv - Genetics 2022Quote: ... lysis of cells and subsequent isolation of total RNA was carried out using the lysing matrix B/FastPrep® sample preparation system (MP Biomedicals) and the miRNeasy Mini Kit (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: The zebrafish larvae collected in Lysing Matrix Y tubes were homogenized with 3 cycles of 15 s at 6,500 RPM with rest intervals of 60 s by FastPrep™ 24-5G tissue-cell homogenizer (MP Biomedicals, USA). The homogenates were clarified by centrifugation at 9,000 × g for 5 min and RNA was extracted using RNeasy Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: The cells were fixed in 4% paraformaldehyde (Wako) and then permeabilized using 0.3% Triton X-100 (MP Biomedicals; Santa Ana, CA, USA). After blocking the cells by incubation in PBS containing 1% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in 20 µL of [1 mg/mL] 100T zymolyase (cat. no. 08320932, MP Biomedicals or cat. no. 320932, VWR) and incubated at room temperature for 6-7 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... LNCaP and 22Rv1 cells were transduced at a multiplicity of infection (MOI) of 0.5 and selected with 1 µg/ml puromycin (MP Biomedicals Cat #194539) for 7 days ...
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bioRxiv - Cancer Biology 2022Quote: ... This negative selection approach results in isolation of untouched CD8 or CD4 T cells and stored frozen with 10% DMSO (MP Biomedicals, 196055). In total ...
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bioRxiv - Molecular Biology 2024Quote: ... and ectopic protein expression in Flp-In T-REx HeLa cells was induced with 1 μg/mL doxycycline (MP Biomedicals, 195044, Dox) minimum three hours post-seeding ...
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bioRxiv - Cell Biology 2024Quote: HEK293A or NG2-OSBP cells were seeded at approximately 50% confluence on 20 µg/ml entactin-collagen-laminin coated 8-well glass slides (MP biomedicals ICN6040805) and returned to the incubator ...
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bioRxiv - Neuroscience 2024Quote: ... A confluent 75 mm flask of PC12 cells was diluted 1:3 and plated in a 10 cm dish containing a multi-test 8-well slide (MP Biomedicals # 20190128). 24 hours later cells were transfected with 5 μg pacAd5CMV-CRECFP and
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bioRxiv - Physiology 2021Quote: For pulse labeling of newly translated proteins MIN6 cells were incubated in complete DMEM media but without cysteine and methionine (MP Biomedicals, #SKU 091646454) for 1hr ...